Ferritin-tagged ulva polysaccharide lyase for efficient degradation of biomass polysaccharides into reducing sugars.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2025-05-28 DOI:10.1007/s00449-025-03177-7

Qing Yang, Wenhui Jin, Hua Fang, Weizhu Chen, Quanling Xie, Hui Chen, Qian Liu, Xian Jiang, Shaohua Wang, Longtao Zhang, Yiping Zhang, Zhuan Hong

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Abstract

Enhancing the stability and the reusability of ulva polysaccharide lyase (UPL) is crucial for the efficient production of reducing sugars from ulva polysaccharides, which are vital for their broad applications in functional foods. In this study, we innovatively developed a self-immobilized UPL by fusing the enzyme with ferritin, leading to the spontaneous formation of micron-sized ulva polysaccharide lyase supraparticles (mUPLSPs). This novel system streamlines the enzyme purification and immobilization process into a single step, effectively circumventing the need for conventional, laborious chromatographic methods. The mUPLSPs exhibited superior stability and reusability, maintaining over 80% of their initial activity after five cycles of use. When compared to free UPLs, mUPLSPs displayed enhanced thermal and pH stability, resulting in a 252% increase in the yield of reducing sugars after a 40-hour reaction period. The ferritin-tagged, self-immobilization strategy not only provides a scalable and cost-efficient approach to the sustainable production of reducing sugars from ulva polysaccharides but also holds significant potential for industrial-scale applications.

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铁蛋白标记的ulva多糖裂解酶有效地将生物质多糖降解为还原糖。

提高ulva多糖裂解酶（UPL）的稳定性和可重复利用性，对于从ulva多糖中高效生产还原糖至关重要，这对其在功能食品中的广泛应用至关重要。在这项研究中，我们创新地开发了一种自固定的UPL，通过将酶与铁蛋白融合，导致自发形成微米级的ulva多糖裂解酶超颗粒（mUPLSPs）。这种新型系统将酶纯化和固定化过程简化为一个步骤，有效地避免了传统的、费力的色谱方法的需要。muplsp表现出优异的稳定性和可重用性，在5个循环使用后保持了80%以上的初始活性。与游离ups相比，mUPLSPs表现出更强的热稳定性和pH稳定性，在40小时的反应周期后，还原糖的产量增加了252%。铁蛋白标记的自固定策略不仅为从ulva多糖中可持续生产还原糖提供了一种可扩展且具有成本效益的方法，而且具有工业规模应用的巨大潜力。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.