Bioprocess and Biosystems Engineering最新文献_第6页

Synergistic effects of ionic liquid-solvent systems on geniposide cinnamoylation catalyzed by whole-cell biocatalysts. 离子液体-溶剂体系对全细胞生物催化剂催化栀子苷肉桂酰化的协同作用。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-03-09 DOI: 10.1007/s00449-026-03309-7

Ziling Huang, Rongling Yang, Xiangjie Zhao, Chun Zhu, Yuli Tong, Xiaoyan Li, Hongzhen Luo

引用次数: 0

Editorial Expression of Concern: Cerium oxide nanoparticles: green synthesis using Banana peel, cytotoxic effect, UV protection and their photocatalytic activity. 编辑表达关注：氧化铈纳米颗粒：香蕉皮绿色合成，细胞毒性作用，紫外线防护及其光催化活性。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-03-04 DOI: 10.1007/s00449-026-03304-y

Abdolhossein Miri, Honeyeh Beiki, Ahmad Najafidoust, Mehrdad Khatami, Mina Sarani

引用次数: 0

Integrated one-pot ultrasound-assisted method for carotenoid extraction from Rhodosporidium toruloides. 超声辅助一体化一锅法提取桃红孢子虫类胡萝卜素。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-03-04 DOI: 10.1007/s00449-026-03299-6

Lachi Wankhede, Gaurav Bhardwaj, Carlos Saul Osorio-González, Satinder Kaur Brar

引用次数: 0

Solid state fermentation of Ginkgo Biloba leaves and its effects on antioxidant capacity and breast muscle quality of broilers. 银杏叶固态发酵及其对肉鸡抗氧化能力和胸肌品质的影响

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-03-01 Epub Date: 2026-01-10 DOI: 10.1007/s00449-025-03279-2

Qi Li, Shufeng Gao, Fuqiang Zhang, Ran Chen, Xuhui Zhang, Linguo Zhao

{"title":"Solid state fermentation of Ginkgo Biloba leaves and its effects on antioxidant capacity and breast muscle quality of broilers.","authors":"Qi Li, Shufeng Gao, Fuqiang Zhang, Ran Chen, Xuhui Zhang, Linguo Zhao","doi":"10.1007/s00449-025-03279-2","DOIUrl":"10.1007/s00449-025-03279-2","url":null,"abstract":"Ginkgo biloba leaves contain many kinds of active ingredients, which can affect the metabolism of animals to a certain extent, thus promoting the growth performance and disease prevention and resistance of animals. After microbial fermentation, Ginkgo biloba leaves can better retain and release their active ingredients, and achieve full utilization of biomass, making them suitable as feed additives. In this study, we conducted an orthogonal optimization experiment with 7 factors and 3 levels using Ginkgo biloba leaves as the substrate, employing two probiotic strains Bacillus licheniformis and B. natto as the fermentation strains, respectively. The results indicated that the fermented leaves were enriched with probiotics and demonstrated enhanced levels of flavonoids, total amino acids, crude protein and aroma substances. Specifically, the flavonoid content increased by 16.7% and 10.6%, total amino acids by 17.22% and 31.05%, total protein by 43.83% and 58.74%, and protease activities reached 1307 U/g and 1510 U/g, for B. licheniformis and B. natto, respectively. Furthermore, supplementation with 0.3-0.6% (mass fraction) of the Ginkgo biloba leaf fermentation product in broiler diets significantly improved the feed efficiency of brolier chickens at 21-42 d and 1-42 day. Through biological solid-state fermentation and conversion, the biological activity, nutritional value, and palatability of Ginkgo biloba leaves were significantly enhanced. When used as a feed additive for Ginkgo biloba leaves, it promoted the development of thymus and spleen, improved the antioxidant capacity of broiler chickens, significantly increased the pH of breast muscle for 24 h, reduced shear force, and significantly reduced the level of cooking loss of breast muscle, thus expanding a new way for efficient and high-value utilization of Ginkgo biloba leaves.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"651-665"},"PeriodicalIF":3.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145948555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of a low-adhesion metallic cell culture surface by nanosecond laser processing. 用纳秒激光加工制备低粘附金属细胞培养表面。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-03-01 Epub Date: 2025-12-20 DOI: 10.1007/s00449-025-03268-5

Kaisei Ito, Atsushi Ezura, Hideharu Shimozawa, Yoshikatsu Akiyama, Chikahiro Imashiro, Jun Komotori

{"title":"Fabrication of a low-adhesion metallic cell culture surface by nanosecond laser processing.","authors":"Kaisei Ito, Atsushi Ezura, Hideharu Shimozawa, Yoshikatsu Akiyama, Chikahiro Imashiro, Jun Komotori","doi":"10.1007/s00449-025-03268-5","DOIUrl":"10.1007/s00449-025-03268-5","url":null,"abstract":"In this study, we developed a reusable low-adhesion metallic cell culture surface having microscale structures using nanosecond pulsed laser processing. Titanium alloy disks were mirror-polished and laser-processed to create microstructures with a pitch of 15 μm, smaller than typical cell size. The cytocompatibility of the developed surfaces was confirmed, showing comparable viability to standard plastic dishes. On the other hand, the cells on the laser-processed surfaces exhibited suppressed lamellipodia formation and maintained a rounded morphology and the area of adhered cells was significantly inhibited compared to polished surfaces, indicating reduced adhesion. Further, by applying PBS jet flow to the culture surface, it has been demonstrated that the cells on the micro-structured surfaces formed significantly larger detachment zones under PBS jet flow, confirming weakened adhesion strength. Furthermore, intact cell sheets could be detached from the laser-processed surfaces by pipetting, whereas cells on polished surfaces remained adherent. These results suggest that the developed culture surface enables on-demand cell detachment through physical stimuli without enzymatic treatment, maintaining cell-cell junctions and extracellular matrix integrity. This technology offers potential for applications in cell sheet engineering and enzyme-free cell harvesting, contributing to cost-effective and sustainable cell-based applications. Future work should investigate cell proliferation and migration behavior to further validate its utility for industrial tissue engineering platforms.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"505-515"},"PeriodicalIF":3.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13021703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145793202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of iron-based materials on anaerobic digestion of thermally hydrolyzed sewage sludge: methane production and speciation of potentially toxic elements. 铁基材料对热水解污泥厌氧消化的影响：甲烷的产生和潜在有毒元素的形成。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-03-01 Epub Date: 2026-01-10 DOI: 10.1007/s00449-025-03280-9

Luiza Usevičiūtė, Tomas Januševičius, Vaidotas Danila, Mantas Pranskevičius, Aušra Mažeikienė, Alvydas Zagorskis, Eglė Marčiulaitienė

{"title":"Effects of iron-based materials on anaerobic digestion of thermally hydrolyzed sewage sludge: methane production and speciation of potentially toxic elements.","authors":"Luiza Usevičiūtė, Tomas Januševičius, Vaidotas Danila, Mantas Pranskevičius, Aušra Mažeikienė, Alvydas Zagorskis, Eglė Marčiulaitienė","doi":"10.1007/s00449-025-03280-9","DOIUrl":"10.1007/s00449-025-03280-9","url":null,"abstract":"This study investigated the effects of iron-based materials-microscale zero-valent iron (mZVI), nanoscale zero-valent iron (nZVI), and nanoscale magnetite (nano-Fe3O4, in two size ranges: 50[Formula: see text]100 nm and 14[Formula: see text]29 nm)-on the anaerobic digestion (AD) of thermally hydrolyzed sewage sludge (THSS). Batch experiments were conducted under mesophilic conditions with three dosages (5, 15, and 30 mg/g-TS) of each material. Methane production kinetics were analyzed using the modified Gompertz model. A sequential extraction procedure was used to assess the speciation of potentially toxic elements (PTEs), namely, Zn, Cu, Pb, Ni, and Cr, in the digestates. The results showed that both mZVI and nZVI enhanced cumulative CH4 production more than either size of nano-Fe3O4. The highest cumulative CH4 yields (223 mL/g-VSadded), approximately 9% higher than the control, were achieved at nZVI dosages of 5 and 15 mg/g-TS. Among iron-based materials, nZVI most effectively shortened the lag phase (1.6-fold decrease at 15 mg/g-TS), whereas both sizes of nano-Fe3O4 had minimal effect (maximum 1.06-fold decrease for the 50-100 nm Fe3O4 at 30 mg/g-TS). The addition of mZVI and nZVI increased the mobility of Zn, Cu, and Ni in the digested THSS samples, while both nano-Fe3O4 materials reduced mobility of all studied PTEs. Overall, the results indicate a trade-off between enhanced methane production and environmental risk; mZVI and nZVI improve AD but increase PTE mobility, whereas nano-Fe3O4 mitigates PTE mobility with little or no effect on CH4 production.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"667-690"},"PeriodicalIF":3.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13021819/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145948519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sterilization strategy determines degradation efficacy of diverse lignocellulosic biomasses by Irpex lacteus. 灭菌策略决定了乳酸菌对多种木质纤维素生物质的降解效果。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-03-01 Epub Date: 2026-01-03 DOI: 10.1007/s00449-025-03274-7

Ruling Yuan, Qingbai Wu, Jinping Li, Dongze Niu, Tingzhou Lei, Taoli Huhe

{"title":"Sterilization strategy determines degradation efficacy of diverse lignocellulosic biomasses by Irpex lacteus.","authors":"Ruling Yuan, Qingbai Wu, Jinping Li, Dongze Niu, Tingzhou Lei, Taoli Huhe","doi":"10.1007/s00449-025-03274-7","DOIUrl":"10.1007/s00449-025-03274-7","url":null,"abstract":"Biological pretreatment by white-rot fungi is a promising green technology for lignocellulose valorization, yet its industrial application is hampered by the high energy consumption of conventional steam sterilization. While a hurdle technology strategy combining low-temperature pasteurization and pH control has proven effective for wheat straw, its general applicability across diverse agricultural residues remains unclear. This study systematically compared the effects of low-temperature pasteurization (70 °C and 80 °C) with autoclaving (121 °C) on the pretreatment of wheat, rice, and rapeseed straw by Irpex lacteus at an initial pH of 4.5. The results revealed significant substrate-specific responses. For wheat and rapeseed straw, pasteurization achieved enzymatic saccharification yields comparable to or exceeding those of autoclaving, demonstrating excellent energy-saving potential. In contrast, rice straw required autoclaving at 121 °C to achieve maximum delignification (55.91% acid-insoluble lignin loss) and subsequent sugar release, likely due to its more recalcitrant native microbiota and structure. Pyrolysis-Gas Chromatography-Mass Spectrometry (Py-GC/MS) analysis confirmed that the S/G ratio decreased after pretreatment. Correlation analysis further established that the S/G ratio had a stronger negative correlation with glucose yield (r = - 0.94) than the total acid-insoluble lignin (AIL) content (r = - 0.90). These results suggest that the selective degradation of lignin structure, particularly the removal of S-units, is more closely associated with hydrolysis efficiency than total lignin removal. This work validates the viability of a low-temperature biological pretreatment strategy and underscores the necessity of tailoring protocols to specific feedstock characteristics to achieve efficient and economical biomass conversion.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"571-582"},"PeriodicalIF":3.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145896261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable biosynthesis of exopolysaccharides derived from agricultural byproducts with effective antioxidant properties. 从农业副产品中提取的具有有效抗氧化特性的外多糖的可持续生物合成。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-03-01 Epub Date: 2026-02-04 DOI: 10.1007/s00449-025-03273-8

Wenfeng Mo, Cuiwen Jian, Ailing Deng, Jiamin Xu, Lijia Liu, YunXia Bai, Shubo Li

{"title":"Sustainable biosynthesis of exopolysaccharides derived from agricultural byproducts with effective antioxidant properties.","authors":"Wenfeng Mo, Cuiwen Jian, Ailing Deng, Jiamin Xu, Lijia Liu, YunXia Bai, Shubo Li","doi":"10.1007/s00449-025-03273-8","DOIUrl":"10.1007/s00449-025-03273-8","url":null,"abstract":"Exopolysaccharides (EPS) are natural macromolecular carbohydrates with good functional activity and physiological activities, but their industrial application was limited by high production costs and unclear structure-function relationships. This study developed a circular economy strategy to produce EPS via microbial fermentation using two food processing wastes (cane molasses and soy sauce residue). After optimizing fermentation medium, the waste-based system achieved an EPS yield of 33.06 ± 0.54 g/L. Two heteropolysaccharides-EPS-2 and EPS-3 were successfully isolated and purified. Monosaccharide composition analysis revealed that EPS-2 primarily consisted of arabinose, glucose, and galactose in a ratio of 65.35:20.82:13.83. In contrast, EPS-3 exhibited a more complex profile containing rhamnose (33.54%), galactose (31.62%), fucose (17.93%), arabinose (13.47%), and glucose (3.44%). Notably, EPS-3 demonstrated higher antioxidant activity than EPS-2. This study successfully demonstrates an innovative waste-to-value conversion strategy that not only achieves high-value utilization of discarded resources but also establishes the fundamental theoretical framework for scalable production of renewable biopolymers.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"559-569"},"PeriodicalIF":3.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the biological mechanisms of biohydrogen production through dark fermentation using assembled genomes from metagenomic data. 利用来自宏基因组数据的组装基因组揭示通过暗发酵生产生物氢的生物学机制。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-03-01 Epub Date: 2025-12-27 DOI: 10.1007/s00449-025-03267-6

Patricia Bovio-Winkler, Esteban Orellana, Stefano Campanaro, José de Jesús Montoya-Rosales, Lucas Tadeu Fuess, Julián Carrillo-Reyes, Elena Castelló, Karla M Muñoz-Páez, Iván Moreno-Andrade, Germán Buitrón, Elías Razo-Flores, Claudia Etchebehere

{"title":"Unraveling the biological mechanisms of biohydrogen production through dark fermentation using assembled genomes from metagenomic data.","authors":"Patricia Bovio-Winkler, Esteban Orellana, Stefano Campanaro, José de Jesús Montoya-Rosales, Lucas Tadeu Fuess, Julián Carrillo-Reyes, Elena Castelló, Karla M Muñoz-Páez, Iván Moreno-Andrade, Germán Buitrón, Elías Razo-Flores, Claudia Etchebehere","doi":"10.1007/s00449-025-03267-6","DOIUrl":"10.1007/s00449-025-03267-6","url":null,"abstract":"Dark fermentation represents a sustainable and promising approach for biohydrogen generation. However, achieving high yields depends on understanding the complex microbial interactions driving the process. This study used genome-centric metagenomics to analyze microbial communities from 11 hydrogen-producing reactors. In total, 44 metagenome-assembled genomes (MAGs) were analyzed in detail. High-yield reactors demonstrated a strong synergy between hydrogen-producing bacteria (HPB) and lactic acid bacteria (LAB), particularly Clostridium butyricum and Clostridium beijerinckii. These species encode the electron-transferring flavoprotein-lactate dehydrogenase complex (EtfAB-ldh complex), enabling hydrogen production from lactic acid. In contrast, reactors with lower hydrogen yields exhibited a higher prevalence of hydrogenotrophic microorganisms, including homoacetogens and methanogens, which redirected electron flow toward competing pathways, thereby decreasing hydrogen output. These results emphasize the importance of promoting HPB while suppressing hydrogen consumers to maintain an optimal microbial community. By linking community composition with metabolic potential, this study provides a framework for improving reactor performance, increasing hydrogen yields, and advancing sustainable hydrogen production from organic waste streams.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"489-503"},"PeriodicalIF":3.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145843583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kinetic and thermodynamic evaluation of lipid and pigment extraction from Nannochloropsis oculata by ultrasound-assisted and conventional methods. 超声辅助和常规方法提取纳米绿藻脂质和色素的动力学和热力学评价。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-03-01 Epub Date: 2026-01-20 DOI: 10.1007/s00449-025-03271-w

Filipe Echart de Oliveira, Nauro da Silveira, Luiz Antonio de Almeida Pinto, Tito Roberto Sant'Anna Cadaval, Anelise Christ-Ribeiro, Débora Pez Jaeschke

引用次数: 0