Bioprocess and Biosystems Engineering最新文献_第6页

Research on the impact of polydopamine hydrogel electrodes with various doping methods on the performance of microbial fuel cells. 研究不同掺杂方式的聚多巴胺水凝胶电极对微生物燃料电池性能的影响。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-06-01 Epub Date: 2025-04-19 DOI: 10.1007/s00449-025-03154-0

Jinhu Ma, Ye Chen, Qing Wen

{"title":"Research on the impact of polydopamine hydrogel electrodes with various doping methods on the performance of microbial fuel cells.","authors":"Jinhu Ma, Ye Chen, Qing Wen","doi":"10.1007/s00449-025-03154-0","DOIUrl":"10.1007/s00449-025-03154-0","url":null,"abstract":"Microbial fuel cells (MFCs) have attracted considerable interest as a promising bioelectrochemical technology for directly converting chemical energy into electrical energy. However, their performance remains limited by the properties of anode materials and their interactions with microbial communities. In this study, PPy-MXene/PDA and PDA-PPy-MXene composite hydrogel electrodes (PMP and PPM) were fabricated on a conductive carbon felt substrate to systematically evaluate the influence of different PDA doping strategies on electrode performance. The PMP electrode exhibited a maximum power density of 3.62 W/m2, which represented a 34.6% increase compared to the PPM electrode (2.69 W/m2). Moreover, the protein content on the PMP electrode reached 38.05 ± 4.88 mg/cm2, 3.79 times higher than that on the PPM electrode (10.05 ± 3.05 mg/cm2). High-throughput sequencing of the 16S rRNA gene revealed that the relative abundance of Geobacter on the PMP electrode surface reached 73.66%, significantly higher than the 51.17% observed on the PPM electrode. These results are attributed to the PDA doping method involving secondary deposition on the electrode surface. This method optimizes the electron transfer pathways and significantly enhances the electrode's conductivity and electrochemical activity by altering the surface roughness of the electrode and increasing the content of hydrophilic functional groups. Consequently, it significantly promotes the enrichment of electroactive microorganisms and improves the efficiency of extracellular electron transfer. This study optimized PDA doping strategies to significantly enhance the electrochemical performance of MFCs, providing new insights and approaches for the rational design of high-performance bioelectrochemical electrodes.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"951-970"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-purpose Bacillus subtilis fermentation: enhanced nattokinase production via oxygen-enriched fed-batch cultivation and natto starter preparation from harvested biomass. 双重用途枯草芽孢杆菌发酵：通过富氧分批培养和从收获的生物质制备纳豆发酵剂来增强纳豆激酶的生产。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-06-01 Epub Date: 2025-05-01 DOI: 10.1007/s00449-025-03151-3

Jiawen Zheng, Yaping Sun, Yunyu Liao, Peng Qin, Rongzhen Che, Jing-Yi Zhao, Zijun Xiao

{"title":"Dual-purpose Bacillus subtilis fermentation: enhanced nattokinase production via oxygen-enriched fed-batch cultivation and natto starter preparation from harvested biomass.","authors":"Jiawen Zheng, Yaping Sun, Yunyu Liao, Peng Qin, Rongzhen Che, Jing-Yi Zhao, Zijun Xiao","doi":"10.1007/s00449-025-03151-3","DOIUrl":"10.1007/s00449-025-03151-3","url":null,"abstract":"Nattokinase (NK) is one of the most important functional components in natto, but its content is low. In this study, the fermentation conditions using Bacillus subtilis JZ08-02 for high-yield NK production were investigated, and the residual bacterial pellets were used to prepare a natto starter. Batch fermentation of NK was conducted using a 5 L fermenter, and soybean milk and glucose were used as the substrates. When the stirring speed was increased from 450 to 650 rpm with air supply at 1.0 vvm, NK was increased from 4859 ± 142 to 12,294 ± 226 IU/mL. When pure oxygen was supplied, 15,013 ± 550 IU/mL of NK was obtained. When fed-batch fermentation was conducted, the titer was further elevated to 18,014 ± 112 IU/mL, which was increased by about 76% compared with the previous result. The experimental findings revealed that aeration control and nutrient feeding regimens exerted pronounced effects on NK productivity during submerged fermentation. The crude enzyme supernatant was obtained by centrifugation and the precipitate was collected. With optimized protectant, the bacterial pellets were freeze-dried with 90.1% cell survival rate. Using economical and edible feedstocks, this study achieved a significant enhancement in NK fermentation yield via oxygen-enriched fed-batch cultivation. At the same time, a natto starter was prepared as a by-product using the residual cell waste.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1039-1046"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesized ZnO-based bimetallic nanocomposite for anticancer, antimicrobial, and photocatalytic applications. 生物合成zno基双金属纳米复合材料在抗癌、抗菌和光催化方面的应用。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-06-01 Epub Date: 2025-03-23 DOI: 10.1007/s00449-025-03150-4

Nidal M Hussein, Sobhan Mortazavi-Drazkola

{"title":"Biosynthesized ZnO-based bimetallic nanocomposite for anticancer, antimicrobial, and photocatalytic applications.","authors":"Nidal M Hussein, Sobhan Mortazavi-Drazkola","doi":"10.1007/s00449-025-03150-4","DOIUrl":"10.1007/s00449-025-03150-4","url":null,"abstract":"Industrial wastewater, particularly antibiotic-laden effluents, poses a significant environmental threat, necessitating efficient and sustainable remediation strategies. In this study, ZnO/Au nanocomposites were synthesized using Urtica dioica extract (ZnO/Au@UDE NCs), offering an eco-friendly alternative to conventional chemical methods. The NCs exhibited well-defined spherical and oval morphologies (40-50 nm), as confirmed by FTIR, TEM, and XRD analyses. Their photocatalytic efficiency in degrading penicillin G was optimized by adjusting key parameters, achieving rapid degradation within 130 min. The incorporation of gold nanoparticles significantly enhanced the electron-hole separation, thereby improving photocatalytic performance. Furthermore, ZnO/Au@UDE NCs demonstrated potent antibacterial activity against Escherichia coli and Pseudomonas aeruginosa (MIC: 125 µg/ml) and exhibited strong antioxidant and anticancer properties, with IC50 values of 72.49 µg/ml for MCF-7 and 23.63 µg/ml for AGS cancer cells. The combined photocatalytic and biological functionalities highlight the potential of these NCs for environmental remediation and biomedical applications, demonstrating a sustainable and multifunctional approach to nanomaterial development.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"913-926"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and bioactive functionalization of bioinks for 3D bioprinting. 用于3D生物打印的生物墨水的遗传和生物活性功能化。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-20 DOI: 10.1007/s00449-025-03180-y

Pawan Kumar, Jitender Sharma, Ravinder Kumar, Jan Najser, Jaroslav Frantik, Nagaraju Sunnam, Anil Sindhu, Seepana Praveenkumar

{"title":"Genetic and bioactive functionalization of bioinks for 3D bioprinting.","authors":"Pawan Kumar, Jitender Sharma, Ravinder Kumar, Jan Najser, Jaroslav Frantik, Nagaraju Sunnam, Anil Sindhu, Seepana Praveenkumar","doi":"10.1007/s00449-025-03180-y","DOIUrl":"https://doi.org/10.1007/s00449-025-03180-y","url":null,"abstract":"3D bioprinting is revolutionizing tissue engineering and regenerative medicine by enabling the precise fabrication of biologically functional constructs. At its core, the success of 3D bioprinting hinges on the development of bioinks, hydrogel-based materials that support cellular viability, proliferation, and differentiation. However, conventional bioinks face limitations in mechanical strength, biological activity, and customization. Recent advancements in genetic engineering have addressed these challenges by enhancing the properties of bioinks through genetic modifications. These innovations allow the integration of stimuli-responsive elements, bioactive molecules, and extracellular matrix (ECM) components, significantly improving the mechanical integrity, biocompatibility, and functional adaptability of bioinks. This review explores the state-of-the-art genetic approaches to bioink development, emphasizing microbial engineering, genetic functionalization, and the encapsulation of growth factors. It highlights the transformative potential of genetically modified bioinks in various applications, including bone and cartilage regeneration, cardiac and liver tissue engineering, neural tissue reconstruction, and vascularization. While these advances hold promise for personalized and adaptive therapeutic solutions, challenges in scalability, reproducibility, and integration with multi-material systems persist. By bridging genetics and bioprinting, this interdisciplinary field paves the way for sophisticated constructs and innovative therapies in tissue engineering and regenerative medicine.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental bioremediation of pharmaceutical residues: microbial processes and technological innovations: a review. 药物残留的环境生物修复：微生物过程和技术创新：综述。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-01 Epub Date: 2025-01-06 DOI: 10.1007/s00449-024-03125-x

Upeksha Gayangani Jayasekara, Tony Hadibarata, Dwi Hindarti, Budi Kurniawan, Mohammad Noor Hazwan Jusoh, Paran Gani, Inn Shi Tan, Adhi Yuniarto, Rubiyatno, Mohd Hairul Bin Khamidun

{"title":"Environmental bioremediation of pharmaceutical residues: microbial processes and technological innovations: a review.","authors":"Upeksha Gayangani Jayasekara, Tony Hadibarata, Dwi Hindarti, Budi Kurniawan, Mohammad Noor Hazwan Jusoh, Paran Gani, Inn Shi Tan, Adhi Yuniarto, Rubiyatno, Mohd Hairul Bin Khamidun","doi":"10.1007/s00449-024-03125-x","DOIUrl":"10.1007/s00449-024-03125-x","url":null,"abstract":"The ubiquitous presence of pharmaceuticals and personal care products (PPCPs) in the environment has become a significant concern due to their persistence, bioaccumulation potential in biota, and diverse implications for human health and wildlife. This review provides an overview of the current state-of-the-art in environmental bioremediation techniques for reducing pharmaceutical residues, with a special emphasis on microbial physiological aspects. Numerous microorganisms, including algae, bacteria or fungi, can biodegrade various pharmaceutical compounds such as antibiotics, analgesics and beta-blockers. Some microorganisms are capable of transferring electrons within the cell, and this feature can be harnessed using Bio Electrochemical Systems (BES) to potentiate the degradation of pharmaceuticals present in wastewater. Moreover, researchers are evaluating the genetic modification of microbial strains to improve their degradation capacity and expand list of target compounds. This includes also discuss how environment changes, such as fluctuations in temperature or pH, may affect bioremediation efficiency. Furthermore, the presence of pharmaceuticals in the environment is emphasised as a major public health issue because it increases the chance for antibiotic-resistant bacteria emerging. This review combines existing information and outlines needed research areas for improving bioremediation technologies in the future.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"705-723"},"PeriodicalIF":3.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic algorithm-optimized artificial neural network for multi-objective optimization of biomass and exopolysaccharide production by Haloferax mediterranei. 遗传算法优化的人工神经网络多目标优化地中海黄颡鱼生物量和胞外多糖产量。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-01 Epub Date: 2025-03-22 DOI: 10.1007/s00449-025-03143-3

Alaa M Al Rawahi, Mohd Zafar, Taqi Ahmed Khan, Sara Al Araimi, Biswanath Mahanty, Shishir Kumar Behera

{"title":"Genetic algorithm-optimized artificial neural network for multi-objective optimization of biomass and exopolysaccharide production by Haloferax mediterranei.","authors":"Alaa M Al Rawahi, Mohd Zafar, Taqi Ahmed Khan, Sara Al Araimi, Biswanath Mahanty, Shishir Kumar Behera","doi":"10.1007/s00449-025-03143-3","DOIUrl":"10.1007/s00449-025-03143-3","url":null,"abstract":"Microbial production of industrially important exopolysaccharide (EPS) from extremophiles has several advantages. In this study, key media components (i.e., sucrose, yeast extract, and urea) were optimized for biomass growth and extracellular EPS production in Haloferax mediterranei DSM 1411 using Box-Behnken design. In a multi-objective optimization framework, response surface methodology (RSM) and genetic algorithm (GA)-optimized artificial neural network (ANN) were used to minimize biomass growth while increasing EPS production. The performance of the selected ANN model for the prediction of biomass and EPS (R2: 0.964 and 0.975, respectively) was found to be better than that of the multiple regression model (R2: 0.818, 0.963, respectively). The main effect of sucrose and its interaction with urea appears to have a significant effect on both responses. The ANN model projects an increase in EPS production from 4.49 to 18.2 g l-1 while shifting the priority from biomass to biopolymer. The optimized condition predicted a maximum biomass and EPS production of 17.27 g l-1 and 17.80 g l-1, respectively, at concentrations of sucrose (19.98 g l-1), yeast extract (1.97 g l-1), and urea (1.99 g l-1). Based on multi-objective optimization, the GA-ANN model predicted an increase in the EPS to biomass ratio for increasing the EPS and associated biomass production. The extracted EPS, identified as Gellan gum through NMR spectroscopy, was further characterized for surface and elemental composition using SEM-EDX analysis.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"785-798"},"PeriodicalIF":3.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient enzymatic hydrolysis of sweet potato residue by fed-batch method to prepare high- concentration glucose. 用补料分批法高效酶解甘薯渣制备高浓度葡萄糖。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-01 Epub Date: 2025-03-25 DOI: 10.1007/s00449-025-03146-0

Shaoyu Wang, Jialong Xu, Shuai Xu, Yuxiang Li, Zhongbo Sun, Dahai Li, Yaohong Ma, Juanjuan Qian, Liping Tan, Tongjun Liu

{"title":"Efficient enzymatic hydrolysis of sweet potato residue by fed-batch method to prepare high- concentration glucose.","authors":"Shaoyu Wang, Jialong Xu, Shuai Xu, Yuxiang Li, Zhongbo Sun, Dahai Li, Yaohong Ma, Juanjuan Qian, Liping Tan, Tongjun Liu","doi":"10.1007/s00449-025-03146-0","DOIUrl":"10.1007/s00449-025-03146-0","url":null,"abstract":"China is the largest producer and exporter of sweet potato in the world. Sweet potato residue (SPR) separated after starch extraction account for more than 10% of the total dry matter of sweet potatoes. However, large amounts of unutilized SPR can cause environmental pollution. SPR is rich in starch and cellulose, both of which can be converted into glucose, making it a good carbon source for microbial fermentation. Therefore, an efficient SPR enzymatic process needs to be developed. The technological conditions of high-solid enzymatic hydrolysis of SPR by fed-batch was investigated in detail. Cellulase, amylase, and pectinase had synergistic effects on SPR enzymatic digestion. The experiments were first conducted to optimize the total enzyme addition of 15 mg enzyme protein/g substrate. The experiments were designed using Design-Expert (10.0) to optimize the enzyme proportions to 42%, 31.8%, and 26.2% for cellulase, amylase, and pectinase, respectively. The fed-batch enzymatic hydrolysis of SPR was investigated. The feed time and amount were optimized. The results showed that the initial SPR enzymatic hydrolysis concentration was 14% (w/v), 9% (w/v) was added at 3 h, 6 h and 12 h, respectively and the final substrate concentration was 41% (w/v). After 24 h of enzymatic hydrolysis, the glucose concentration obtained was 194.57 g/L and the glucan conversion was 63.58%. The fed-batch enzymatic hydrolysis of SPR described in this study has great potential for the whole chain utilization of sweet potato and in the microbial fermentation industry as it is environmentally friendly, economical and efficient.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"829-839"},"PeriodicalIF":3.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ synthesis of silver nanoparticles on silk: producing antibacterial fabrics. 在蚕丝上原位合成纳米银：生产抗菌织物。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-01 Epub Date: 2025-03-03 DOI: 10.1007/s00449-025-03138-0

Xiao-Qian Jin, Jia-Lei Li, Jie Liu, Liang-Liang Chen, Chan Liu, Ya-Qing Zhou, Wen-Pu Shi, Huan Liang, Wei-Hong Guo, Da-Chuan Yin

{"title":"In situ synthesis of silver nanoparticles on silk: producing antibacterial fabrics.","authors":"Xiao-Qian Jin, Jia-Lei Li, Jie Liu, Liang-Liang Chen, Chan Liu, Ya-Qing Zhou, Wen-Pu Shi, Huan Liang, Wei-Hong Guo, Da-Chuan Yin","doi":"10.1007/s00449-025-03138-0","DOIUrl":"10.1007/s00449-025-03138-0","url":null,"abstract":"Herein, we explored an effective method for preparing silver nanoparticles (Ag NPs)-coated antibacterial silk fabrics. In particular, using amino acids and cellulose from silk as reducing agents and silver nitrate as a precursor, Ag NPs were synthesised in situ on the surface of silk without requiring additional reducing agents and catalysts. The surface morphology and chemical composition of the involved samples were characterised using techniques such as scanning electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy. Notably, silk and silk precursors (silkworm cocoons, silk fibers and sericin) could be used for in situ Ag NPs synthesis. Furthermore, the antibacterial properties of the samples were evaluated against Escherichia coli-a Gram-negative bacterium-as a model, demonstrating an impressive antibacterial rate of up to 99.91%. In addition, we investigated the water absorption behaviour of the samples at 25 °C by assessing their moisture regain, water retention value and vertical wick height. The results indicated that the Ag NPs coating did not damage the water absorption performance of the involved silk. Finally, we compared the fabric performance before and after treatment using a universal testing machine and colorimeter. The results showed that the mechanical properties of the fabrics with the Ag NPs coating did not substantially change with treatment, but the fabrics became more yellowish. Overall, this research has notable application potential in the field of antibacterial fabrics.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"725-736"},"PeriodicalIF":3.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hybrid dynamic flux balance modeling approach for bioprocesses: an E. coli case study. 生物过程的混合动态通量平衡建模方法：大肠杆菌案例研究。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-01 Epub Date: 2025-03-25 DOI: 10.1007/s00449-025-03147-z

Zahra Negahban, Valerie Ward, Anne Richelle, Chris McCready, Hector Budman

{"title":"Hybrid dynamic flux balance modeling approach for bioprocesses: an E. coli case study.","authors":"Zahra Negahban, Valerie Ward, Anne Richelle, Chris McCready, Hector Budman","doi":"10.1007/s00449-025-03147-z","DOIUrl":"10.1007/s00449-025-03147-z","url":null,"abstract":"In this study, we present a hybrid dynamic flux balance analysis (DFBA) model, combined with Partial Least Squares (PLS) regression, to simulate cell culture behavior in response to variations in media composition. DFBA models typically incorporate a stoichiometric matrix representing metabolic reactions, leveraging the pseudo-stationarity assumption to reduce the number of parameters, which in turn minimizes the risk of overfitting. Here, PLS regression is employed to define kinetic rate constraints within the DFBA model, capturing the dynamic and non-linear nature of reaction rates over different culture phases. An optimization approach identifies the minimal number of kinetic constraints required, ensuring model accuracy without excessive complexity. Our hybrid model is validated through simulation case studies using an E. coli system, demonstrating its effectiveness in adjusting to changes in initial media composition. The case studies reveal that the model's accuracy improves with a more detailed stoichiometric matrix, particularly when larger networks or more varied metabolic environments are present. Additionally, the hybrid DFBA-PLS approach provides a robust and scalable modeling framework adaptable to other bioprocesses, offering insights into medium composition effects and highlighting its potential for bioprocess optimization.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"841-856"},"PeriodicalIF":3.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A tandem conversion process for the synthesis of polyitaconic acid from glucose in the Aspergillus terreus culture. 陆地曲霉培养中葡萄糖合成多衣康酸的串联转化工艺。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-01 Epub Date: 2025-03-11 DOI: 10.1007/s00449-025-03139-z

Ikumi Kinoshita, Tomonari Tanaka, Yuji Aso

{"title":"A tandem conversion process for the synthesis of polyitaconic acid from glucose in the Aspergillus terreus culture.","authors":"Ikumi Kinoshita, Tomonari Tanaka, Yuji Aso","doi":"10.1007/s00449-025-03139-z","DOIUrl":"10.1007/s00449-025-03139-z","url":null,"abstract":"In this study, a bio-based vinylidene monomer, itaconic acid (IA), produced by a fungus Aspergillus terreus NBRC 6123 was polymerized in the culture. The inhibition of IA polymerization by the culture components was eased by extraction with 4-methyltetrahydropyran (4mTHP). The extraction with 4mTHP for 4 days under aerobic condition resulted in IA production at 67.9 g/L (522 mM) in the organic phase from 180 g/L glucose. Interestingly, 4mTHP did not affect to the glucose consumption and respiration of A. terreus. IA polymerization in 4mTHP for 72 h using 400 mM IA and 1 mM 2,2'-azobis(4-methoxy-2,4-dimethylvaleronitrile) as a radical initiator under anaerobic condition by N2 gas purging resulted in that the IA conversion, and the weight-averaged molecular weight (Mw) and the molecular weight distribution (MWD) of the synthesized polyitaconic acid (polyIA) were 11.5%, 79.8 × 103 g/mol, and 1.26, respectively. The respiration by the IA producer itself was used to remove the residual oxygen in the flask by sealing after the IA production. Colorimetric evaluation with a redox indicator, resazurin, revealed that an anaerobic condition which is suitable for the IA polymerization can be accomplished by just incubation for 1 day after the sealing. The synthesis of polyIA from glucose in the same flask with 4mTHP was demonstrated, resulting in that the IA conversion, and Mw and MWD of the synthesized polyIA were 13.8%, 9.1 × 103 g/mol, and 1.24, respectively, when 47.1 g/L (362 mM) IA was produced by the extraction for 3 days. To our knowledge, this is the first demonstration of radical polymerization of vinylidene monomer in microbial cultures.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"737-748"},"PeriodicalIF":3.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0