Biochemistry and Biophysics Reports最新文献

{"title":"The potential of efferocytosis for the treatment of bronchial asthma: A review of current trends, mechanisms and prospects","authors":"Baohe Liu ,&nbsp;Tingting Zu ,&nbsp;Yaqi Lu ,&nbsp;Chaopin Xing ,&nbsp;Meng Gao ,&nbsp;Fuling Wu ,&nbsp;Mengqi Jiang","doi":"10.1016/j.bbrep.2025.102161","DOIUrl":"10.1016/j.bbrep.2025.102161","url":null,"abstract":"<div><div>As a chronic inflammatory disease of the lungs, bronchial asthma is closely associated with three key characteristics: airway inflammation, airway hyperresponsiveness, and airway remodeling. The long-term infiltration of multiple inflammatory cells into the airway interstitium results in damage to the airway epithelial barrier. Macrophages, as the primary immune cells responsible for the clearance of damaged and apoptotic cells from the asthmatic airways, phagocytose asthmatic cells through a series of efferocytosis phases, including the “find me,” “eat me,” and “digest” phases. This process, which involves the phagocytosis of apoptotic inflammatory and epithelial cells, serves to reduce airway epithelial damage and protect immune homeostasis. However, the cytosolic burial process of macrophages in asthma patients often exhibits dysfunctions, including reduced phagocytic efficiency, disorders of cytosolic burial signaling molecules, and the activation of airway inflammation. These impede the clearance process of inflammatory cells from the airway epithelium and necessitate the formation of temporary protective barriers. Barriers are formed through epithelial-mesenchymal transition, which impairs the regenerative capacity of the damaged epithelium and its barrier function, leading to an imbalance in epithelial-mesenchymal homeostasis. This, in turn, results in the occurrence of airway remodeling, which further exacerbates the process of asthma development. Intact and efficient efferocytosis serves as a critical regulatory mechanism in maintaining inflammatory homeostasis and suppressing epithelial-mesenchymal transition. Therapeutic modulation of macrophage-mediated efferocytosis represents a promising strategy for bronchial asthma intervention. In this paper, we present an overview of the specific stages of efferocytosis and the molecular mechanisms underlying the defects in efferocytosis.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102161"},"PeriodicalIF":2.3,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell RNA sequencing reveals altered immune and stromal landscape in primary and liver metastasis of gastric cancer","authors":"Bin Zhou ,&nbsp;Xiaolin Liu ,&nbsp;Huanhuan Hu ,&nbsp;Zhipeng Li ,&nbsp;Shanliang Zhong","doi":"10.1016/j.bbrep.2025.102163","DOIUrl":"10.1016/j.bbrep.2025.102163","url":null,"abstract":"<div><h3>Background</h3><div>We aimed to identify stromal cells associated with liver metastasis of gastric cancer (GC).</div></div><div><h3>Methods</h3><div>We downloaded single-cell RNA sequencing (scRNA-seq) datasets for GC tissues and adjacent normal tissues (NT), as well as liver metastases (LM) and healthy liver tissues (HL) from GEO. Additionally, we obtained RNA-Seq and clinical data from TCGA for the TCGA-STAD project. The datasets were analyzed using R software. Cell viability analysis were used to verify the potential drugs.</div></div><div><h3>Results</h3><div>We obtained 7 GC, 2 NT, 5 LM and 3 HL datasets, as well as 448 RNA-Seq files. We identified 13 cell types from a total of 107,875 cells, which were further re-clustered into 38 subclusters. Our analyses showed that both GC and LM exhibited increased angiogenesis, cancer-associated fibroblasts (CAFs) were enriched in primary tumors, CD8⁺ T cells in both GC and LM showed a declined proportion and increased necroptosis, NK cells were reduced in LM, and M2 macrophages (Mφ) exhibited high activities in both GC and LM. We found immune cells in HL and LM expressed more transcripts, suggesting the differences in tumor microenvironment (TME) between primary and metastatic tumors. Additionally, we identified 3 potential drugs that could be effective against both primary and metastatic tumors.</div></div><div><h3>Conclusion</h3><div>This study illustrated the heterogeneity of immune and stromal cells in TME of GC and LM, and identified several cell types associated with liver metastasis of GC. These cell types may serve as potential biomarkers or therapeutic targets in the future.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102163"},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoma-derived growth factor and non-coding RNA network in ovarian cancer patients","authors":"Reza Ganjali ,&nbsp;Setare Nassiri ,&nbsp;Narges Zamani ,&nbsp;Zeinab Shaker-ardekani ,&nbsp;Mohammad Elahimanesh ,&nbsp;Nazanin Hosseinkhan ,&nbsp;Mitra Nourbakhsh","doi":"10.1016/j.bbrep.2025.102168","DOIUrl":"10.1016/j.bbrep.2025.102168","url":null,"abstract":"<div><h3>Background</h3><div>Ovarian cancer (OC) remains the most lethal gynecologic malignancy due to late diagnosis and limited effective biomarkers. Hepatoma-derived growth factor (HDGF) has emerged as an oncogene implicated in tumor progression, yet its regulation and clinical potential in OC remains underexplored. Recent evidence suggests that long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) may modulate oncogenic pathways through competing endogenous RNA (ceRNA) networks.</div></div><div><h3>Methods and results</h3><div>Fifty ovarian cancer and fifty normal ovarian tissue samples were analyzed for HDGF, miR-345-5p, and LINC00839 expression using qRT-PCR. Bioinformatic tools were employed to predict RNA-RNA interactions and reconstruct a ceRNA network. Statistical analyses examined expression correlations and diagnostic performance via ROC curve. HDGF and LINC00839 were significantly upregulated, while miR-345-5p was downregulated in OC tissues (p &lt; 0.001). HDGF expression correlated positively with LINC00839 (r = 0.70) and inversely with miR-345-5p (r = −0.58), suggesting a regulatory axis where LINC00839 sponges miR-345-5p to derepress HDGF. Elevated HDGF levels were associated with larger tumor size, advanced FIGO stage, and metastasis. ROC analysis revealed that HDGF serum level (AUC = 0.88) has promising diagnostic potential, albeit lower than CA-125 and HE4.</div></div><div><h3>Conclusion</h3><div>Our study highlights the LINC00839–miR-345-5p–HDGF axis as a key regulatory network in ovarian cancer. HDGF may serve as a clinically relevant biomarker for disease progression, while LINC00839 and miR-345-5p represent promising therapeutic targets. These findings provide a foundation for future investigations into ceRNA-based diagnostics and treatments in OC.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102168"},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implications of lipoprotein (a) [Lp(a)] gene polymorphic sequence variations and its protein expression in venous thromboembolism (VTE)","authors":"Karan Happa ,&nbsp;Arshad A. Pandith ,&nbsp;Umar H. Khan ,&nbsp;Shayaq Ul Abeer Rasool ,&nbsp;Aabid M. Koul ,&nbsp;Qurat Ul Ain ,&nbsp;Mir Uzair ul Haq ,&nbsp;Usma Manzoor ,&nbsp;Adil Lateef ,&nbsp;Rafi A. Jan","doi":"10.1016/j.bbrep.2025.102152","DOIUrl":"10.1016/j.bbrep.2025.102152","url":null,"abstract":"<div><h3>Background</h3><div>Venous thromboembolism (VTE) is a significant global health concern, with an annual incidence of approximately 1–2 per 1000 individuals. VTE is a major cause of morbidity and mortality worldwide, leading to substantial healthcare costs due to hospitalizations, long-term anticoagulation therapy, recurrent thrombotic events and the need for lifelong clinical management. The burden of VTE is particularly pronounced in aging populations, with incidence rates increasing exponentially after the age of 50. Despite its significant impact, the genetic underpinnings of VTE remain incompletely understood. This study investigates the association of Lp(a) gene polymorphisms (93C &gt; T and 121G &gt; A) and Lp(a) expression with VTE risk.</div></div><div><h3>Methods</h3><div>A case-control study was conducted, enrolling 101 VTE cases and 110 healthy, age- and gender-matched controls. Genotyping was performed using PCR-RFLP, and serum Lp(a) levels were quantified using ELISA. Genotype distribution and their association with VTE risk was determined by statistical analyses.</div></div><div><h3>Results</h3><div>The +121 G &gt; A polymorphism exhibited a significant protective effect, with the GA genotype more prevalent in controls than cases (OR = 0.41, 95 % CI = 0.23–0.73, p = 0.002). Additionally, the combined GA + AA genotypes were significantly associated with a lower VTE risk (OR = 0.44, p = 0.003). Serum Lp(a) levels were elevated in VTE cases (mean VTE 36.41 mg/dl) compared to controls (mean 32.00 mg/dl, p &lt; 0.84). Notably, this difference was most pronounced in the 30–70 mg/dl subgroup (p &lt; 0.01). D-Dimer was significantly elevated in VTE (p &lt; 0.001) and GA + AA genotype was significantly more frequent in control subjects with D-Dimer levels &lt;500 ng/ml (OR = 0.23, 95 % CI = 0.05–0.95, p = 0.04. LP (a)+121 G &gt; A variant genotype was significantly higher in controls for males, smokers and BMI ≤25 than VTE cases (p &lt; 0.05). No significant difference was found in LP(a) +93C &gt; T with VTE risk.</div></div><div><h3>Conclusion</h3><div>Our study concludes the inverse role of Lp(a) polymorphic variation +121 G &gt; A in the pathogenesis of VTE. +93C &gt; T variant and serum lipoprotein (a) levels did not increase the risk of pathogenesis of VTE. However, gender specific impact of LP(a) on VTE is plausible.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102152"},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HbA1c levels in hemoglobin H disease","authors":"Pasiri Kerdsinchai ,&nbsp;Adisak Tantiworawit ,&nbsp;Worapaka Manosroi ,&nbsp;Piangrawee Niprapan ,&nbsp;Sirichai Srichairatanakool ,&nbsp;Teerachat Punnachet ,&nbsp;Nonthakorn Hantrakun ,&nbsp;Pokpong Piriyakhuntorn ,&nbsp;Thanawat Rattanathammethee ,&nbsp;Sasinee Hantrakool ,&nbsp;Chatree Chai-Adisaksopha ,&nbsp;Ekarat Rattarittamrong ,&nbsp;Lalita Norasetthada ,&nbsp;Kanda Fanhchaksai ,&nbsp;Pimlak Charoenkwan","doi":"10.1016/j.bbrep.2025.102165","DOIUrl":"10.1016/j.bbrep.2025.102165","url":null,"abstract":"<div><h3>Background</h3><div>Patients with beta-thalassemia have been shown to exhibit lower HbA1c levels, often correlating with reduced hemoglobin (Hb) concentrations. Similarly, individual with alpha-thalassemia, particularly those with hemoglobin H (HbH) disease, experience chronic hemolytic anemia, which may also influence HbA1c measurements. This study aimed to investigate the effect of alpha-thalassemia on HbA1c levels.</div></div><div><h3>Methods</h3><div>This cross-sectional study was conducted between September 2022 and April 2024 at the Tertiary care University Hospital. Non-diabetic patients with alpha-thalassemia (hemoglobin H disease) were enrolled and compared with age- and sex-matched control without thalassemia. Statistical analysis was performed using independent t-tests based on distribution of data. Linear regression analysis was used to assess the association between HbH disease and HbA1c levels.</div></div><div><h3>Results</h3><div>A total of 92 participants were enrolled, comprising 46 patients with HbH disease and 46 matched controls. The mean ± SD HbA1c levels were significantly lower in the HbH group (4.09 ± 0.64 %) compared to the control group (5.39 ± 0.50 %) (P &lt; 0.001). The mean difference in HbA1c levels between the two groups was −1.31 ± 0.12 % [95 % CI -1.54 to −1.07](P &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>Patients with HbH exhibit significantly lower HbA1c levels compared to control group. These finding highlight the need to establish specific HbA1c reference ranges for patients with thalassemia to avoid misinterpretation in the diagnosis and management of diabetes mellitus.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102165"},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of the TLR signaling pathway in the pathogenesis of glioblastoma multiforme with an emphasis on immunotherapy","authors":"Seyedeh Elham Norollahi ,&nbsp;Kosar Babaei ,&nbsp;Ali Rashidy-pour ,&nbsp;Bahman Yousefi ,&nbsp;Rasoul Baharlou ,&nbsp;Bahareh Farasati Far ,&nbsp;Amir Jalali ,&nbsp;Ali Akbar Samadani","doi":"10.1016/j.bbrep.2025.102149","DOIUrl":"10.1016/j.bbrep.2025.102149","url":null,"abstract":"<div><div>The most malignant brain tumor, glioblastoma multiforme (GBM), has a high mortality rate. Recently, translational elements in GBM therapy have emerged as novel therapeutic strategies in addition to conventional treatment methods. In this way, Toll-like receptor (TLR), PI3K/Akt/mTOR, MAPK/ERK, NOTCH, and other signaling pathways have recently become some of the main signaling pathways in brain tumors. The immunological reactions to brain tumors are mediated by these mechanisms. A family of proteins known as TLRs is essential to the natural defense mechanism because it can identify and react to infections and other danger signals. TLRs have dual functions in the glioma microenvironment including that they can initially activate the innate and adaptive immune responses that support antitumor activity and secondly, their activation can also contribute to tumor progression by promoting inflammation and immune evasion, as they are expressed on both immune cells and tumor cells. TLR agonists are receiving more attention in the treatment of glioma because some of them have demonstrated survival benefits in clinical studies when used in conjunction with immunotherapy, chemotherapy, radiation therapy, and immune checkpoint inhibitors. The most exciting use of TLR agonists is that they can be used as immunomodulators to avoid dose accumulation, boost the efficiency of other therapies, and, by upregulating PD-1, reinforce delayed immune checkpoint resistance against PD-1/PD-L1 inhibition. Therefore, the use of TLR agonists can lead to PD-L1 overexpression, which in turn enhances the efficacy of checkpoint inhibitors and triggers potent anticancer immune responses. In this article, we describe the function of the TLR signaling system, the cellular and molecular elements contributing to the etiology of glioblastoma multiforme, the connection between TLRs and glioma, and their significance for immunotherapy.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102149"},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alteration of enzyme activities and MYb10 gene expression in response to brown rot disease in two apple cultivars","authors":"Mohammad Reza Raji ,&nbsp;Sepideh Sanjari ,&nbsp;Fatemeh Derikvand ,&nbsp;Mitra Khademi ,&nbsp;Mostafa Farajpour","doi":"10.1016/j.bbrep.2025.102166","DOIUrl":"10.1016/j.bbrep.2025.102166","url":null,"abstract":"<div><div>Apples (<em>Malus domestica</em>) are widely enjoyed but are prone to fungal infections, notably brown rot caused by Monilinia spp., which significantly impacts postharvest quality. This study evaluated the activities of catalase (CAT), peroxidase (POD), phenylalanine ammonia lyase (PAL), and polyphenol oxidase (PPO), along with the expression of <em>MdMYB10</em> in two apple cultivars (Gala Imperial and Lebanese) after inoculation with <em>Monilinia laxa</em> over 1, 2, and 7 days. Results showed that enzyme activities and <em>MdMYB10</em> expression significantly increased post-infection, indicating their roles in the defense against brown rot. Notably, a strong positive correlation was found between <em>MdMYB10</em> upregulation and the activities of PAL, POD, and PPO. PAL activity peaked at 2 days post-inoculation, while <em>MdMYB10</em> expression, along with POD and PPO activities, reached their highest levels by day 7. These findings suggest that <em>MdMYB10</em> expression enhances the activities of these defense enzymes, with PAL initiating the response by producing phenolic compounds that serve as substrates for PPO and POD, aiding in disease resistance. Although PAL, POD, and PPO activities increased after <em>M. laxa</em> inoculation, no significant differences in PPO and POD activities were observed between cultivars, while Gala Imperial displayed higher PAL activity, enhancing its resistance to infection. The study highlights <em>MdMYB10</em> as a potential candidate for breeding programs aimed at improving brown rot resistance in apples.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102166"},"PeriodicalIF":2.3,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Up-regulation of NGEF via the BRAFV600E /ERK/AP1 pathway enhances invasion and migration abilities of BRAFV600E-mutant thyroid cancer","authors":"Zhao Fu ,&nbsp;Ye Wang ,&nbsp;Cong-Jun Wang ,&nbsp;Zhu Yu ,&nbsp;Jun-Qiang Chen","doi":"10.1016/j.bbrep.2025.102164","DOIUrl":"10.1016/j.bbrep.2025.102164","url":null,"abstract":"<div><div>The BRAF^V600E mutation is one of the most common genetic alterations in thyroid tumors, and intrinsic feedback mechanisms have limited the clinical application of BRAF^V600E-specific inhibitors. This study aims to investigate the potential biological function of the downstream overexpressed molecule NGEF following the BRAF^V600E mutation and its regulatory mechanisms. By integrating data from the CEO database, TCGA database, and clinical samples, we found that NGEF is highly expressed in thyroid cancer and is positively correlated with tumor size, local lymph node metastasis, clinical stage, and disease-free survival. Intriguingly, analysis of TCGA data revealed that NGEF expression is significantly higher in BRAF^V600E-mutant thyroid cancers. Subsequent validation demonstrated that NGEF expression is markedly elevated in BRAF^V600E-mutant cancer cell lines and BRAF^V600E-engineered cellular models compared to normal cells and BRAF^V600E-negative cancer cells. Functional experiments, pathway enrichment analysis, and investigations into phenotype-associated biomarkers further revealed that NGEF promotes invasion and migration of BRAF^V600E-mutant thyroid cancer cells through the epithelial-mesenchymal transition (EMT) pathway. To explore the regulatory relationship between the BRAF^V600E mutation and NGEF expression, we used bioinformatics tools to predict transcription factors, conducted pathway inhibition experiments, and performed dual-luciferase reporter assays. These studies confirmed that BRAF^V600E regulates NGEF expression via the ERK/AP1 pathway. These findings suggest that NGEF enhances the invasive and migratory abilities of BRAF^V600E-mutant thyroid cancer cells through BRAF^V600E/ERK/AP1 upregulation and may serve as a potential therapeutic target for BRAF^V600E-mutant thyroid cancer cells.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102164"},"PeriodicalIF":2.3,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-loaded simvastatin-ginsenoside Rh2 liposomes enhance cellular immune responses as vaccine adjuvants","authors":"Yinxiu Huang ,&nbsp;Qingqing Nie ,&nbsp;Zhijie Chen ,&nbsp;Wang Gong ,&nbsp;Wenqiang Cao ,&nbsp;Xue Wang ,&nbsp;Yanna Liu ,&nbsp;Ziyan Meng","doi":"10.1016/j.bbrep.2025.102159","DOIUrl":"10.1016/j.bbrep.2025.102159","url":null,"abstract":"<div><div>Vaccine adjuvants are pivotal in novel vaccine development, enhancing immunization and modulating immune responses. This study prepared liposomes loaded with simvastatin and ginsenoside Rh2 (LIPO-SIM-Rh2) via high-speed shearing, rotary evaporation, and high-pressure homogenization. Using C57BL/6J mice, six groups were established to explore its immune-enhancing effects and mechanisms. Following two-dose immunization at 0 and 21 days, IgG antibody titers (ELISA), immune cell responses (flow cytometry), and safety/function of antigen-presenting cells (pathological sections, immunofluorescence staining) were detected. Results showed LIPO-SIM-Rh2 had a stable dispersion and a near-100 % encapsulation efficiency. Compared with other groups, it significantly increased anti-OVA specific IgG titer, promoted cellular immune response, up-regulated MHC-I molecule antigen presentation pathway, activated macrophages, promoted dendritic cell homing, and caused no obvious damage or inflammation to major organs. In conclusion, LIPO-SIM-Rh2 adjuvant has a feasible preparation method and strong immune-enhancing potential for vaccines.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102159"},"PeriodicalIF":2.3,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An innovative compound that promotes oral wound healing via mobilizing gingival mesenchymal stem cell homing","authors":"Lei Yang ,&nbsp;Yue Song ,&nbsp;Yao Jiao ,&nbsp;Siyan Liu ,&nbsp;Yi Liu ,&nbsp;Lijia Guo ,&nbsp;Yitong Liu","doi":"10.1016/j.bbrep.2025.102154","DOIUrl":"10.1016/j.bbrep.2025.102154","url":null,"abstract":"<div><div>Oral wound healing is a complex biological process involving inflammation, proliferation, and tissue remodeling. Gingival mesenchymal stem cells (GMSCs) play a crucial role in this process due to their regenerative potential and immunomodulatory properties. This study investigated whether Purpurolide C (PC), a fungal-derived bioactive compound, could promote oral wound healing by promoting the migratory ability of GMSCs. In vitro, PC enhanced GMSC migration, pluripotency, and osteogenic differentiation. In vivo, a mouse palatal soft tissue defect model demonstrated that PC significantly accelerated wound closure and increased GMSC recruitment to the wound site. Furthermore, 16S rRNA sequencing revealed that PC administration did not disrupt gut microbiota homeostasis. These findings suggest that PC is a promising therapeutic agent for enhancing oral wound healing, paving the way for potential clinical applications in regenerative medicine.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102154"},"PeriodicalIF":2.3,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}