Biochemistry and Biophysics Reports最新文献

{"title":"Duchesnea indica (Andr.) Focke extracts exerted anti-inflammatory effect via inhibiting MAPK/ERK pathway in LPS-stimulated RAW264.7 cells","authors":"Haipeng Feng ,&nbsp;Qianqian Qiao ,&nbsp;Jingyan Zhang,&nbsp;Kang Zhang,&nbsp;Lei Wang,&nbsp;Jianxi Li","doi":"10.1016/j.bbrep.2025.102273","DOIUrl":"10.1016/j.bbrep.2025.102273","url":null,"abstract":"<div><div><em>Duchesnea indica</em> (Andr.) Focke (<strong>DIF</strong>), a medicinal plant from the Rosaceae family, possesses therapeutic properties such as heat-clearing and detoxification, dispersion of stasis detumescence, blood-cooling, and hemostatic effect. The latest research unveiled a diverse array of pharmacologically active compounds from DIF, showcasing a broad spectrum of pharmacological effects. However, there was limited attention given to the comprehensive investigation of its high molecular weight compounds, particularly polysaccharides. In this study, DIF extracts were prepared by water-extraction and alcohol-precipitation method and the pharmacological effect was detected. The results demonstrated that the total polysaccharide content, reducing sugar content, total flavonoid content, and total polyphenol content in DIF extracts were 32.62 ± 0.91 %, 13.41 ± 0.18 %, 1.07 ± 0.07 %, and 12.16 ± 0.27 %, respectively. Although the total antioxidant activity of DIF extracts were significantly low than that of the vitamin C group, its ability to scavenge ABTS, DPPH, and superoxide anion radicals was similar to that of the vitamin C group. Furthermore, DIF extracts demonstrated significant inhibition on NO and MDA levels while simultaneously enhancing SOD activity in LPS-stimulated RAW264.7 cells. Finally, DIF extracts significantly reduced the mRNA level of pro-inflammation cytokines interleukin (<strong>IL</strong>)-6, IL-1β, inducible nitric oxide synthase (<strong>iNOS</strong>), cyclooxygenase-2 (<strong>COX-2</strong>) and tumor necrosis factor (<strong>TNF</strong>)<strong>-α</strong>, and directly inhibited the phosphorylation level of extracellular regulated kinase and mitogen-activated protein kinase (<strong>ERK-MAPK</strong>) pathway. Taken together, these results indicated that DIF extracts exhibited an anti-inflammatory and antioxidant effect in LPS-induced RAW264.7 mouse macrophages by directly inhibiting ERK-MAPK signaling pathway. Based on these findings, DIF extracts provided new insights into the treatment and prevention of diseases related to oxidative stress and inflammation.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102273"},"PeriodicalIF":2.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the ABTS-based antioxidant potential and antiproliferative activity of pulp, skin and seeds extracts from Nephelium lappaceum, Manilkara zapota, and Meliccocus oliviformis on human prostate and colon cancer cells","authors":"Adelma Escobar-Ramírez ,&nbsp;José A. González Garrido ,&nbsp;Oswaldo Hernández Abreu ,&nbsp;Edgar Zenteno ,&nbsp;Carlos J. Solórzano Mata ,&nbsp;Yobana Pérez Cervera ,&nbsp;Tony Lefebvre ,&nbsp;Vanessa Dehennaut","doi":"10.1016/j.bbrep.2025.102257","DOIUrl":"10.1016/j.bbrep.2025.102257","url":null,"abstract":"<div><div>Despite significant advancements in cancer treatments, cancer remains the second leading cause of death worldwide, creating a pressing need for effective and non-toxic therapies. Plant bioactive compounds, particularly phenolic acids, have shown promising antioxidant and anticancer properties. In this study, we investigated the antioxidant potential and antiproliferative activity of methanolic extracts obtained from the skin, pulp, and seeds of three exotic fruits commonly consumed in Mexico: <em>Manilkara zapota</em>, <em>Nephelium lappaceum</em>, and <em>Meliccocus oliviformis</em>. The antioxidant activity was assessed using the ABTS radical scavenging assay, and total phenolic content was quantified by a modified Folin-Ciocalteu method. Most extracts exhibited significant ABTS radical scavenging capacity, with the highest phenolic content detected in the skin and seeds of <em>N. lappaceum</em>.</div><div>and <em>M. oliviformis</em>. Cytotoxic and antiproliferative effects were evaluated on human colon (HCT116) and prostate (DU145) cancer cell lines. The skin of <em>N. lappaceum</em> and the seeds of <em>M. zapota</em> and <em>M. oliviformis</em> showed strong antiproliferative activity. These findings highlight the therapeutic potential of underutilized fruit by-products and support further investigation into their bioactive constituents as complementary candidates for cancer treatment.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102257"},"PeriodicalIF":2.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of long intergenic non-protein coding RNA 312 in cancer: A bioinformatics and literature based study","authors":"Arash Safarzadeh,&nbsp;Setareh Ataei,&nbsp;Soudeh Ghafouri-Fard","doi":"10.1016/j.bbrep.2025.102283","DOIUrl":"10.1016/j.bbrep.2025.102283","url":null,"abstract":"<div><div>LINC00312 encodes an intronless transcript mainly functioning as a tumor suppressor. Expression of this transcript is downregulated in a variety of cancers, particularly lung cancers. It is regarded as a negative regulator of estrogen receptor signaling. Moreover, low expression of LINC00312 correlates with poor survival in sarcoma and stomach adenocarcinoma, suggesting its potential as a prognostic biomarker. In the current study, we performed a bioinformatics and literature based approach to find the importance of this long non-coding RNA in different cancers, its regulatory network and its interactions with different biomolecules and compounds. Taken together, LINC00312 represents a possible candidate for additional diagnostics and prognostics approaches.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102283"},"PeriodicalIF":2.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HOXA7 can be used for prognostic evaluation and molecular targeted therapy of gliomas: a multidimensional study based on a large sample size","authors":"Zhi Sha ,&nbsp;Zhendong Liu ,&nbsp;Yanzheng Gao","doi":"10.1016/j.bbrep.2025.102279","DOIUrl":"10.1016/j.bbrep.2025.102279","url":null,"abstract":"<div><div><em>HOXA7</em> has been reported to play an important role in various malignant tumors; however, its role in gliomas has not yet been elucidated. This retrospective study aimed to comprehensively investigate the expression patterns, clinical significance, and potential molecular mechanisms of HOXA7 in gliomas through multi-dimensional analysis. Based on a large sample size of 1946 gliomas from various databases, this study used a variety of statistical and bioinformatics methods to explore the role of <em>HOXA7</em> in gliomas at multiple levels (mRNA, cell signaling pathways, clinical characteristics, and prognosis). <em>HOXA7</em> has abnormally high expression in gliomas, and its expression level can be used as an independent prognostic factor for gliomas, as well as having some clinical diagnostic value. In addition, <em>HOXA7</em> may be involved in the regulation of some cancer-related cell signaling pathways, such as pantothenate and CoA biosynthesis. Abnormally high expression of <em>HOXA7</em> may be significantly correlated with multiple clinical features and cancer-related cell signaling pathways, and it may be used as a biomarker and molecular target for evaluating prognosis of gliomas.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102279"},"PeriodicalIF":2.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Usefulness of plasma and apolipoprotein B-depleted serum samples in paraoxonase 1 assessment","authors":"Rina Kawaguchi ,&nbsp;Akira Yoshimoto ,&nbsp;Takahiro Kameda ,&nbsp;Ryunosuke Ohkawa","doi":"10.1016/j.bbrep.2025.102274","DOIUrl":"10.1016/j.bbrep.2025.102274","url":null,"abstract":"<div><div>Paraoxonase 1 (PON1) is closely associated with antioxidant, anti-inflammatory, and antiatherosclerotic functions of high-density lipoprotein (HDL). Although many clinical studies have evaluated relationships between PON1 activity and various diseases based on its multiple functions, their results were contradictory because of the difference of sample preparation methods. Therefore, we investigated an optimal preanalytical method for PON1 analysis by measuring three different PON1 activities in various types of specimens. Samples were prepared from healthy human serum, plasma with or without calcium addition, HDL isolated by ultracentrifugation, and apolipoprotein B-depleted serum (BDS). Using these samples, PON1 protein concentration and activities using three substrate types (<em>p</em>-nitrophenyl acetate, paraoxon, and γ-thiobutyrolactone) were evaluated. PON1 distributions in HDL subfractions from serum and BDS were also investigated. Although PON1 activities in plasma were lower than those in serum, removing EDTA and adding calcium rescued PON1 activities in plasma similar to levels comparable to those in serum. In contrast, HDL isolated by ultracentrifugation had significantly lower PON1 activities and protein concentrations, indicating that many PON1 proteins were not bound to the HDL particle in the HDL fractions collected from serum and plasma by ultracentrifugation. PON1 protein concentration and distributions in BDS showed similar to those in serum sample than those in HDL sample. Furthermore, three types of PON1 activities were differentially affected by sample preparation procedures. The reduction of PON1 activity in BDS differed among individuals and by the activity type. Focusing on each of three different PON1 activities might further enhance the clinical significance of PON1 testing.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102274"},"PeriodicalIF":2.2,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced HBsAg positivity in systemic lupus erythematosus and Sjögren's syndrome: A retrospective comparative study","authors":"Man Li ,&nbsp;Qihang Zhu ,&nbsp;Zhong Fang ,&nbsp;Taihong Huang ,&nbsp;Lei Dai ,&nbsp;Sen Wang","doi":"10.1016/j.bbrep.2025.102275","DOIUrl":"10.1016/j.bbrep.2025.102275","url":null,"abstract":"<div><h3>Background</h3><div>Systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS) are two common systemic autoimmune diseases. Recent studies have suggested that the prevalence of hepatitis B surface antigen (HBsAg) may be lower in patients with SLE compared to the general population; however, the existing evidence remains limited and controversial. A systematic analysis of HBV infection rates and serological marker distributions in patients with SLE and SS may offer important insights into the interaction between autoimmune and antiviral immunity.</div></div><div><h3>Methods</h3><div>We retrospectively analyzed 2421 hospitalized patients with SLE and 2049 with SS at Nanjing Drum Tower Hospital from January 2019 to December 2024. Patients with other autoimmune diseases (including rheumatoid arthritis (RA), ankylosing spondylitis (AS), ulcerative colitis (UC), and Crohn's disease (CD)) and 5927 non-autoimmune hospitalized patients were included as controls. Hepatitis B virus (HBV) serological markers (HBsAg, HBsAb, HBeAg, HBeAb, and HBcAb) and anti-HCV antibodies were tested. We compared the positivity rates of HBV seromarkers across different groups and performed subgroup analyses based on sex, age, and autoantibody profiles.</div></div><div><h3>Results</h3><div>The HBsAg positivity rate was significantly lower in SLE and SS patients compared with non-autoimmune controls (1.2 % vs. 5.2 %, P &lt; 0.0001; 1.7 % vs. 5.2 %, P &lt; 0.0001, respectively). No significant differences were observed in patients with RA, AS, UC, or CD. Stratified analyses revealed consistently lower HBsAg positivity in SLE and SS patients across both sexes and all age groups above 20 years. Further analyses indicated that this phenomenon was not attributable to occult HBV infection, inpatient versus outpatient status, or immunosuppressant use. Notably, high ANA titers and the presence of antibodies such as anti-dsDNA, anti-SSA, and anti-ribosomal P were associated with lower HBsAg positivity. No significant differences in HBsAg positivity were found between patients with or without common complications.</div></div><div><h3>Conclusion</h3><div>SLE and SS patients exhibit consistently lower HBsAg positivity rates, independent of clinical factors, and linked to autoantibody status. These findings suggest disease-specific immune mechanisms that may promote HBV clearance or reduce susceptibility to infection.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102275"},"PeriodicalIF":2.2,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular phospho-signaling map of the enigmatic serine/threonine kinase MAST2","authors":"Isha Fathima ,&nbsp;Althaf Mahin ,&nbsp;Pahal Priyanka,&nbsp;Nazah Naurah Vattoth,&nbsp;Ayishath Nishana,&nbsp;Athira Perunelly Gopalakrishnan,&nbsp;Sowmya Soman,&nbsp;Rajesh Raju","doi":"10.1016/j.bbrep.2025.102277","DOIUrl":"10.1016/j.bbrep.2025.102277","url":null,"abstract":"<div><h3>Background</h3><div>MAST2 (Microtubule-Associated Serine/Threonine Kinase 2) is an enigmatic serine/threonine kinase that is considered to bridge microtubule-associated cytoskeletal architecture through its phospho-regulatory networks. Yet, MAST2 remains a dark horse in the human kinome, as the molecular details on its structure, upstream regulators, and downstream phosphorylation targets remain unknown.</div></div><div><h3>Methods and results</h3><div>To interpret MAST2-linked phospho-signaling dynamics, PubMed-indexed articles were curated based on predefined MeSH terms to obtain global cellular phosphoproteomics datasets. Among 105 class I phosphosites in MAST2 identified across cellular phosphoproteomics datasets, 2 phosphosites, S74 and S148, were represented more abundantly compared to other phosphosites, making them predominant and functionally significant. Expression coregulation analysis, aimed at interpreting phosphosites that consistently show either similar or opposite patterns of expression, was performed by computing the expression patterns of phosphosites in parallel with the predominant MAST2 phosphosite. Their frequencies were then ranked across differential datasets to ensure consistency, and Fisher's exact test was performed to assess the likelihood of the coregulation pattern. Potential biases within the datasets were mitigated using additional cutoffs. Interpreting these datasets, we identified the majority of high-confidence coregulated protein phosphosites of both predominant phosphosites to be involved in transcriptional regulation. This is consistent with reports that a nearby Arg89Gln mutation in MAST2 disrupts its transcriptional regulatory activity. Co-occurrence analysis of phosphosites within MAST2 revealed that these predominant sites tend to co-occur positively and share similarity in expression coregulation patterns with phosphosites in other proteins. Finally, novel upstream kinases that potentially phosphorylate the predominant phosphosites of MAST2, as well as potential downstream substrates that are phosphorylated by MAST2, were also identified from the high-confidence coregulation dataset.</div></div><div><h3>Conclusions</h3><div>We propose that phosphorylations at S74 and S148 of MAST2 are functionally similar, and that these phosphosites are candidate regulatory sites influencing the transcriptional regulatory activity of MAST2.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102277"},"PeriodicalIF":2.2,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Illuminating the therapeutic potential of Schisandrin a against rheumatoid arthritis by targeting ferroptosis: An integrated bioinformatics and experimental study","authors":"Zhifang Yang,&nbsp;Xiaojuan Yin,&nbsp;Sha Yang,&nbsp;Huimin Li,&nbsp;Huimin Wen","doi":"10.1016/j.bbrep.2025.102266","DOIUrl":"10.1016/j.bbrep.2025.102266","url":null,"abstract":"<div><div>Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation and hyperplasia of the synovial membrane. This study aimed to elucidate the anti-inflammatory mechanisms of Schisandrin A (SCHA), a bioactive component from Schisandra chinensis, on RA fibroblast-like synoviocytes (FLSs). Integrated bioinformatics analysis was performed on transcriptomic datasets from RA and normal FLSs as well as SCHA-treated RA FLSs. Enrichment analysis revealed that differentially expressed genes between groups were significantly enriched in the ferroptosis pathway, suggesting SCHA may exert anti-inflammatory effects by inhibiting ferroptosis. Protein-protein interaction network analysis identified Txnrd1, lpcat3 and slc7a11 as key hub genes with pivotal roles in mediating SCHA's effects, and molecular docking and dynamics simulations demonstrated that SCHA directly binds to these proteins with favorable binding affinities through hydrogen bonds and hydrophobic contacts, with stable complex formation confirmed over 100-ns molecular dynamics trajectories. In lipopolysaccharide (LPS)-induced inflamed RA FLSs, SCHA significantly decreased pro-inflammatory cytokines IL-6, TNF-α and IL-1β at both protein and mRNA levels, restored antioxidant enzymes GSH-Px and SOD levels, and reduced lipid peroxidation product MDA, Fe2+ and ROS levels in a dose-dependent manner Transmission electron microscopy confirmed SCHA's dose-dependent protective effects against ferroptosis-induced mitochondrial damage. Our study demonstrates inhibition of ferroptosis via targeting TXNRD1, LPCAT3 and SLC7A11 mediates the anti-inflammatory effects of SCHA on RA FLSs. These findings reveal a novel mechanism for SCHA's therapeutic potential against RA by modulating ferroptosis.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102266"},"PeriodicalIF":2.2,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of adalimumab Fab variants with the CH1 domain replaced by the Cα1 domain of IgA","authors":"Rara Sugimoto ,&nbsp;Hitomi Nakamura ,&nbsp;Masato Kiyoshi ,&nbsp;Akiko Ishii-Watabe ,&nbsp;Naoko Oda-Ueda ,&nbsp;Takatoshi Ohkuri","doi":"10.1016/j.bbrep.2025.102269","DOIUrl":"10.1016/j.bbrep.2025.102269","url":null,"abstract":"<div><div>Antibody constant domains (C domains) contribute to structural stability. However, studies focusing on Fab fragments with heterologous constant domains are limited. Here, we engineered an IgA-type Fab (Fab_CH1IgA) by replacing the CH1 domain of an IgG1-type adalimumab Fab with the corresponding Cα1 domain of IgA1. Fab_CH1IgA was expressed in CHO cells at approximately 50 mg L⁻¹ and purified to homogeneity. DSC showed comparable thermal stability, with <em>T</em>_m = 74.8 °C for Fab_CH1IgA and 75.2 °C for the IgG1-type Fab. SPR analysis showed similar antigen-binding kinetics, with <em>K</em>_D = 2.23 n M for Fab_CH1IgA and 1.77 nM for the IgG1-type Fab. Structural analysis identified Pro124 and Tyr211 in the C domain as part of the hydrophobic core-bridging variable and constant domains. Substitution of these residues with their IgG-type counterparts reduced thermal stability, underscoring the critical contribution of V–C domain interactions. Although the sequence identity between the IgG1 and IgA1 constant domains was not particularly high, the CH1 domain in adalimumab Fab could be replaced by the IgA Cα1 domain without markedly compromising stability or activity. These findings highlight cooperative packing at the V–C interface, offering important insights into Fab engineering to enhance function while preserving biophysical integrity, and supporting the design of IgG–IgA chimeric antibodies and novel chimeric Fab formats.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102269"},"PeriodicalIF":2.2,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterologous expression and foldase-assisted refolding of LipGoM, a Pseudomonas lipase from family I","authors":"Elena Lizbeth Garcia-Villegas ,&nbsp;Itzel Anahí Hidalgo-Manzano ,&nbsp;Liliana Pardo-López ,&nbsp;Enrique Rudiño-Piñera","doi":"10.1016/j.bbrep.2025.102256","DOIUrl":"10.1016/j.bbrep.2025.102256","url":null,"abstract":"<div><div>Lipases from <em>Pseudomonas</em> species are valuable biocatalysts, but their heterologous expression is often complicated by the need for different protocols to ensure proper folding and activity. In this study, we successfully produced a soluble and active form of LipGoM, a lipase derived from <em>Pseudomonas</em> sp., that employs a refolding strategy after solubilization in urea. Our findings indicate that refolding LipGoM in the presence of its native foldase, LifGoM, is essential for restoring its enzymatic activity. Furthermore, we identified that optimizing the refolding conditions, specifically pH, ionic strength, protein concentration, and the addition of glycerol, reducing agents, and ions, was critical for success. The lipase-lipid GoM mixture, Lip-Lif GoM, exhibited maximal hydrolytic activity toward <em>p</em>-nitrophenyl-octanoate at 55 °C and pH 8.0, demonstrating notable stability in the presence of detergents and organic solvents. These results demonstrate the indispensable role of its cognate foldase, LifGoM, in activating LipGoM, as well as the significance of buffer composition, particularly glycerol, for the effective refolding of the enzyme and its subsequent activity.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102256"},"PeriodicalIF":2.2,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}