Biochemistry and Biophysics Reports最新文献_第3页

The impact of Fc glycosylation on IgG susceptibility to hinge region chemical reduction: implications for the development of immunoassays Fc糖基化对IgG对铰链区化学还原的敏感性的影响：对免疫测定发展的影响

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-25 DOI: 10.1016/j.bbrep.2025.102112

Vanessa Susini, Silvia Ursino, Chiara Sanguinetti, Alice Botti, Laura Caponi, Maria Franzini

{"title":"The impact of Fc glycosylation on IgG susceptibility to hinge region chemical reduction: implications for the development of immunoassays","authors":"Vanessa Susini, Silvia Ursino, Chiara Sanguinetti, Alice Botti, Laura Caponi, Maria Franzini","doi":"10.1016/j.bbrep.2025.102112","DOIUrl":"10.1016/j.bbrep.2025.102112","url":null,"abstract":"<div><div>Antibodies are glycoproteins, and Fc glycosylation plays a critical structural role in maintaining the proper folding of the C<sub>H</sub>2 domain. Deglycosylated IgGs exhibit an open C<sub>H</sub>2 domain conformation that structurally affects also the neighboring hinge region. Selective reduction of disulfide bonds in this region using mild reducing agents generates monovalent thiol-containing IgGs (rIgGs), which can be immobilized on modified surfaces to orient the Fab fragment outward, enhancing antigen binding efficiency and assay sensitivity. This study investigates the effect of Fc glycosylation on IgG chemical reduction and its implications for in-house ELISA and commercial immunoassays. IgGs were enzymatically deglycosylated with Endo S and then reduced to rIgGs by 2-mercaptoethylamine. Deglycosylation and reduction efficiency were verified by non-reducing SDS-PAGE. Glycosylated and deglycosylated rIgGs were tested in in-house ELISA and commercial immunoassays. Results showed that deglycosylation significantly improves rIgG production, probably by increasing hinge-region accessibility to reducing agents. This led to a 20-fold increase in ELISA sensitivity compared to glycosylated rIgGs. Deglycosylation also mitigates batch-to-batch variability in IgG reduction, enabling consistent rIgG yields. These findings highlight the capability of deglycosylation to standardize rIgG production, broadening its applications in diagnostic immunoassays and biosensing technologies.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102112"},"PeriodicalIF":2.3,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144470849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive method of isolating proteins from serum, cerebrospinal fluid, and hippocampal neurons in rats for proteomic profiling using the LC-MS/MS platform 利用LC-MS/MS平台从大鼠血清、脑脊液和海马神经元中分离蛋白质进行蛋白质组学分析的综合方法

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-25 DOI: 10.1016/j.bbrep.2025.102113

Pratibha Sharma, Rajinder K. Dhamija

{"title":"A comprehensive method of isolating proteins from serum, cerebrospinal fluid, and hippocampal neurons in rats for proteomic profiling using the LC-MS/MS platform","authors":"Pratibha Sharma, Rajinder K. Dhamija","doi":"10.1016/j.bbrep.2025.102113","DOIUrl":"10.1016/j.bbrep.2025.102113","url":null,"abstract":"<div><div>Neurology research largely utilizes rat brains due to their structural and functional similarities to humans, making them valuable models for studying various neurological conditions. There is growing interest in investigating diseases such as Alzheimer's disease (AD), Parkinson's disease (PD), cognition, and other mental health-related disorders. This has created a need for a comprehensive, combined, and easy-to-follow method to isolate serum, cerebrospinal fluid (CSF), and hippocampal neurons. The hippocampus, responsible for learning and memory, is affected by various neurological and psychiatric disorders. However, obtaining samples like CSF and hippocampal neurons is challenging, especially from small animals like rats. Currently, there is no efficient method for isolating these samples altogether from a single animal, and its use in downstream applications has not been thoroughly tested. We have developed a comprehensive and streamlined method for isolating serum, CSF, and hippocampal neurons from a single animal, suitable for downstream applications such as proteomics and biomarker research. This method involves using high-speed centrifugation instruments and density gradient centrifugation, which are easy to follow. The isolated proteins were identified through mass spectrometry. Our method has been successfully tested for high-throughput applications with small sample volumes, demonstrating its clinical utility. With our simplified approach, proteins in serum, CSF, and neural cells can be studied simultaneously. The method achieves ease of use, cost-effectiveness, and reproducibility, thereby facilitating a better understanding of neurological disorders.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102113"},"PeriodicalIF":2.3,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144470850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caloric restriction mimetics chlorogenic acid and fisetin as potential autophagy inducers targeting ATG101 热量限制模拟绿原酸和非西汀作为靶向ATG101的潜在自噬诱导剂

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-24 DOI: 10.1016/j.bbrep.2025.102081

Apoorv Sharma , Indu Kumari , Asimul Islam , Hridayesh Prakash , Amresh Prakash , Vijay Kumar

{"title":"Caloric restriction mimetics chlorogenic acid and fisetin as potential autophagy inducers targeting ATG101","authors":"Apoorv Sharma , Indu Kumari , Asimul Islam , Hridayesh Prakash , Amresh Prakash , Vijay Kumar","doi":"10.1016/j.bbrep.2025.102081","DOIUrl":"10.1016/j.bbrep.2025.102081","url":null,"abstract":"<div><div>Autophagy is an important cytoprotective process impaired in neurodegenerative diseases such as Alzheimer's disease. The initiation process is mediated by the protein kinase Unc-51-like kinase 1 (ULK1) complex. ATG101, a cytosolic protein, plays a pivotal role in initiating autophagy as a component of the ULK complex in mammalian cells. It is important to understand the regulatory processes of individual autophagy components under different conditions for the development of therapeutic interventions. The caloric restriction mimetics (CRMs) such as chlorogenic acid (CGA) and fisetin mimic the healthy outcomes of caloric restriction without decreasing caloric consumption, constituting promising therapeutic candidates for neuroprotection. We explored the ATG101 interactions of CGA and fisetin in this work. Molecular docking and molecular dynamics (MD) simulations were used to investigate the interactions of these CRMs with ATG101, evaluating binding stability and dynamics. To confirm these interactions, we conducted quantitative real-time PCR (qRT-PCR) in differentiated SHSY5Y cells, analyzing the effect of CGA and fisetin on ATG101 gene expression. Our results indicated that fisetin forms a more stable complex with ATG101 compared to CGA. Yet, at the transcriptional level, both CRMs stimulate the mRNA level of ATG101. Therefore, these CRMs can be responsible for their potential as autophagy inducers. These findings offer significant insights into the molecular processes through which CRMs may improve neurodegenerative diseases by triggering autophagy.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102081"},"PeriodicalIF":2.3,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melphalan improves toxicity of arsenic trioxide in adult T-cell leukemia/lymphoma cells 美法兰改善三氧化二砷对成人t细胞白血病/淋巴瘤细胞的毒性

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-24 DOI: 10.1016/j.bbrep.2025.102109

Faeze Khodadadi , Mohammad Hadi Sadeghian , Zahra Delbari , Mohadese Kazemi , Hamide Koohpaykar , Fatemeh B. Rassouli

{"title":"Melphalan improves toxicity of arsenic trioxide in adult T-cell leukemia/lymphoma cells","authors":"Faeze Khodadadi , Mohammad Hadi Sadeghian , Zahra Delbari , Mohadese Kazemi , Hamide Koohpaykar , Fatemeh B. Rassouli","doi":"10.1016/j.bbrep.2025.102109","DOIUrl":"10.1016/j.bbrep.2025.102109","url":null,"abstract":"<div><div>Adult T-cell leukemia/lymphoma (ATLL) is a hematologic neoplasm with poor prognosis. Melphalan is an alkylating anti-cancer agent, and arsenic trioxide (ATO) is routine chemotherapy drug for ATLL with low response rate. Due to the significant challenge that chemoresistance poses in treating ATLL, we aimed to investigate the potential of melphalan to enhance the effects of ATO as a combinatorial treatment approach for ATLL.</div><div>MT-2 cells were exposed to different concentrations of melphalan and ATO and viability was evaluated by alamarBlue assay. Upon IC<sub>50</sub> determination, cells were treated with 0.5 μg/ml melphalan and 2 μM ATO for 72 h, and changes induced on the cell cycle were analyzed by PI staining and flow cytometry, while the expression of candidate genes was assessed by quantitative PCR. For <em>in silico</em> analysis, the expression of <em>ABCG2</em> was assessed in MT-2 cells and ATLL subtypes using GEO database, and molecular docking was performed to predict the interaction of melphalan with this drug transporter.</div><div>Melphalan enhanced the cytotoxicity of ATO up to 32.05 %, and caused accumulation of cells in the sub G<sub>1</sub> phase of the cell cycle. Besides, combination of melphalan and ATO induced considerable reduction in <em>c-MYC, BMI-1</em>, <em>CD44</em> and <em>NF-κB</em> (<em>REL-A</em>) expression. Volcano plots revealed the overexpression of <em>ABCG2</em> in MT-2 cells and acute and smoldering ATLL subtypes, and molecular docking indicated favorable affinity of melphalan with ABCG2. Current findings provide valuable insights into the mechanism of action of melphalan and highlight the importance of targeting drug transporters in improving chemotherapy efficacy in ATLL.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102109"},"PeriodicalIF":2.3,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural product-based inhibitors of quorum sensing: A novel approach to combat antibiotic resistance 基于天然产物的群体感应抑制剂：对抗抗生素耐药性的新方法

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-24 DOI: 10.1016/j.bbrep.2025.102111

Esther Ugo Alum , Bashar Haruna Gulumbe , Sylvester Chibueze Izah , Daniel Ejim Uti , Patrick Maduabuchi Aja , Ikechuku Okorie Igwenyi , Christian Emeka Offor

{"title":"Natural product-based inhibitors of quorum sensing: A novel approach to combat antibiotic resistance","authors":"Esther Ugo Alum , Bashar Haruna Gulumbe , Sylvester Chibueze Izah , Daniel Ejim Uti , Patrick Maduabuchi Aja , Ikechuku Okorie Igwenyi , Christian Emeka Offor","doi":"10.1016/j.bbrep.2025.102111","DOIUrl":"10.1016/j.bbrep.2025.102111","url":null,"abstract":"<div><div>The global rise of antibiotic resistance (AR) presents a critical threat to public health, prompting the search for innovative antimicrobial strategies. Quorum sensing (QS), a bacterial communication system that governs virulence, biofilm formation, and resistance, has emerged as a compelling non-lethal therapeutic target. This review explores the potential of natural product-based quorum sensing inhibitors (QSIs) derived from plants, microbes, and marine organisms. These compounds disrupt QS pathways through various mechanisms, including inhibition of signal synthesis, receptor antagonism, enzymatic degradation of signaling molecules, and suppression of QS-regulated gene expression. Particular emphasis is placed on the molecular targets and synergistic potential of natural QSIs when combined with conventional antibiotics to enhance efficacy and curb resistance development. This narrative review explores the diverse sources of natural QSIs, including plant-derived phytochemicals, microbial secondary metabolites, and marine bioactive compounds. In this study, a thorough literature search was conducted using databases such as PubMed, Scopus, Web of Science, and Google Scholar. Studies selected were those published between 2013 and 2025 in peer-reviewed journals. The manuscript also examines translational challenges such as poor bioavailability, bacterial adaptability, and regulatory barriers, alongside innovative strategies including nanoparticle-based delivery and combination therapies. Finally, clinical and industrial applications of QSIs are discussed, reinforcing their promise as sustainable tools for combating resistant infections and biofilm-associated diseases. This review underscores QS inhibition as a transformative approach in the ongoing battle against antibiotic resistance.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102111"},"PeriodicalIF":2.3,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The duplexity of insulin: The integrated bioinformatics analysis and machine learning identified key genes for type 2 diabetes 胰岛素的多重性：综合生物信息学分析和机器学习确定了2型糖尿病的关键基因

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-24 DOI: 10.1016/j.bbrep.2025.102099

Nan Gao , Xiteng Chen , Jun Yang , Yuanfeng Jiang , Shaochong Bu , Xiaomei Bai , Zhenyu Kou , Chunjun Li , Fang Tian

{"title":"The duplexity of insulin: The integrated bioinformatics analysis and machine learning identified key genes for type 2 diabetes","authors":"Nan Gao , Xiteng Chen , Jun Yang , Yuanfeng Jiang , Shaochong Bu , Xiaomei Bai , Zhenyu Kou , Chunjun Li , Fang Tian","doi":"10.1016/j.bbrep.2025.102099","DOIUrl":"10.1016/j.bbrep.2025.102099","url":null,"abstract":"<div><h3>Background</h3><div>Insulin therapy is still the most important treatment for T2DM, but the discussion about whether insulin brings more benefits or harms to T2DM patients has not stopped. Therefore, we used high-throughput RNA sequencing to investigate the role of insulin in T2DM and its molecular changes.</div></div><div><h3>Method</h3><div>We collected peripheral blood samples from 16 patients with T2DM, and performed RNA-seq on peripheral blood mononuclear cells. Bioinformatics analysis and machine learning were uesd to identify the key differential genes and transcription factor networks. In addition, we performed the flow cytometry and staining to observe ROS level and endothelial-monocyte adhesion in PBMCs of both groups.</div></div><div><h3>Results</h3><div>A total of 529 differential genes were identified by bioinformatics analysis. 8 genes were identified as key genes, among which IL-6 had high importance in the random forest model. In transcription factor analysis, IL-6, RETN, CTSG and ELANE have abundant transcriptional regulatory relationships. Flow cytometry showed that ROS production, phagocytosis, leukocyte adhesion in insulin treatment group were lower than that in non-insulin treatment group.</div></div><div><h3>Conclusion</h3><div>Insulin therapy is bidirectional, it can cause islet B cell damage and vascular complications, but also can reduce the level of inflammation and oxidative stress.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102099"},"PeriodicalIF":2.3,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prophylactic role of omega 3 fatty acids in bisphenol F-induced sexual and erectile dysfunction is associated with penile redox homeostasis omega - 3脂肪酸在双酚f诱导的性功能和勃起功能障碍中的预防作用与阴茎氧化还原稳态有关

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-23 DOI: 10.1016/j.bbrep.2025.102103

Adeyemi Fatai Odetayo , Moses Agbomhere Hamed , Grace Edet Bassey , Oluranti Olayinka Titiloye , Samson Daniel Maduabuchi , Kazeem Bidemi Okesina , Luqman Aribidesi Olayaki

{"title":"Prophylactic role of omega 3 fatty acids in bisphenol F-induced sexual and erectile dysfunction is associated with penile redox homeostasis","authors":"Adeyemi Fatai Odetayo , Moses Agbomhere Hamed , Grace Edet Bassey , Oluranti Olayinka Titiloye , Samson Daniel Maduabuchi , Kazeem Bidemi Okesina , Luqman Aribidesi Olayaki","doi":"10.1016/j.bbrep.2025.102103","DOIUrl":"10.1016/j.bbrep.2025.102103","url":null,"abstract":"<div><h3>Introduction</h3><div>Bisphenol F (BPF) is an established environmental pollutant that has been shown to distort erectile function. However, the effect of BPF on penile redox homeostasis is not known. On the other hand, omega-3 fatty acids (O3FA) are known antioxidants with fertility-enhancing properties. Despite these abilities, the ameliorative effect of O3FA on BPF-induced penile redox imbalance is unknown. Hence, this study was designed to add to the existing body of knowledge by investigating the role of penile redox homeostasis on BPF-induced erectile dysfunction and the possible ameliorative effect of O3FA.</div></div><div><h3>Methodology</h3><div>Twenty male Wistar rats were randomly divided into four groups (n = 5/group) after two weeks acclimatization period as follows: control group (.5 ml of corn oil), O3FA group (300mg/kg of O3FA), BPF group (30mg/kg of BPF), and the BPF + O3FA group (30 mg/kg of BPF + 300mg/kg of O3FA).</div></div><div><h3>Results</h3><div>BPF exposure led to sexual and erectile dysfunction, which was accompanied by a disruption in erectogenic enzymatic activities and circulatory testosterone. These events were accompanied by the down-regulation of penile NO/cGMP signaling, oxido-inflammatory response, and penile histoarchitecture distortion. These observed BPF-induced distortions were ameliorated in animals that received O3FA co-treatment with BPF.</div></div><div><h3>Conclusion</h3><div>BPF-induced erectile dysfunction was associated with penile redox imbalance, and O3FA ameliorated BPF-induced penile toxicity.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102103"},"PeriodicalIF":2.3,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144364492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic plasmid clustering in Bacteria: Influence of transcriptional activity and host cellular states 细菌中的动态质粒聚集：转录活性和宿主细胞状态的影响

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-23 DOI: 10.1016/j.bbrep.2025.102108

Guan-Lin Wang, Yi-Ren Chang

{"title":"Dynamic plasmid clustering in Bacteria: Influence of transcriptional activity and host cellular states","authors":"Guan-Lin Wang, Yi-Ren Chang","doi":"10.1016/j.bbrep.2025.102108","DOIUrl":"10.1016/j.bbrep.2025.102108","url":null,"abstract":"<div><div>High-copy-number (hcn) plasmids exhibit distinct spatial organization within bacterial cells, yet the underlying mechanisms and functional implications remain poorly understood. In this study, we systematically investigated the role of transcriptional activity and host cellular states in plasmid clustering. Using fluorescence imaging and quantitative analyses, we observed that transcriptional repression induced transient plasmid clustering, while transcriptional enhancement influenced clustering in a promoter-dependent manner. Additionally, environmental cues, such as glucose addition and growth phase transitions, modulate plasmid clustering independently of transcriptional activity. Glucose supplementation led to rapid plasmid dispersion, suggesting a link between metabolic state and plasmid dynamics. The switching between dispersed and clustered plasmid distributions as growth phase changes correlate with alterations in nucleoid organization. These findings highlight the complex interplay between transcription, nucleoid structure, and cellular state in regulating plasmid spatial distribution, providing new insights into bacterial genome organization and adaptation.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102108"},"PeriodicalIF":2.3,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144364493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered dynamics of T cell subsets in peripheral blood impacts disease progression in newly diagnosed multiple myeloma 外周血T细胞亚群动态改变影响新诊断多发性骨髓瘤的疾病进展

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-23 DOI: 10.1016/j.bbrep.2025.102104

Mohini Vig , Lalit Kumar , Shweta Dubey , Ritu Gupta

引用次数: 0

Single-cell spatial transcriptomics reveals pathogenic mechanism of renal fibrosis in imiquimod-induced lupus nephritis in mice 单细胞空间转录组学揭示吡喹莫德致狼疮性肾炎小鼠肾纤维化的致病机制

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-23 DOI: 10.1016/j.bbrep.2025.102087

Yanan Xing , Yanru An , Tian Tian , Li Pu , Zhigang Lu , Ning Liang , Longqi Liu , Zhouchun Shang

{"title":"Single-cell spatial transcriptomics reveals pathogenic mechanism of renal fibrosis in imiquimod-induced lupus nephritis in mice","authors":"Yanan Xing , Yanru An , Tian Tian , Li Pu , Zhigang Lu , Ning Liang , Longqi Liu , Zhouchun Shang","doi":"10.1016/j.bbrep.2025.102087","DOIUrl":"10.1016/j.bbrep.2025.102087","url":null,"abstract":"<div><div>A deeper understanding of the cellular and molecular pathology pathways of renal fibrosis in lupus nephritis (LN) is essential for the accurate disease assessment and the development of novel therapeutic strategies. In this study, we employed advanced spatial transcriptomics technique to elucidate the underlying mechanism of renal fibrosis in mouse kidneys. By establishing single-nuclei and spatial transcriptomic maps for LN and control kidneys, we identified a significant activation and proliferation of fibroblasts predominantly in the inner stripe of outer medulla (ISOM) region and pinpointed a set of specific gene signatures associated with fibrosis. Furthermore, we discovered a class of pro-fibrotic macrophage subtype, Lyz2 macrophage, that promotes myofibroblast activation. We elucidated the intricate molecular interplay mechanisms involved in this process. Our study also delved into the glomerular region, revealed disease-induced alterations in gene expression and identified potential novel therapeutic targets.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102087"},"PeriodicalIF":2.3,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0