Biochemistry and Biophysics Reports最新文献

Radiation-induced rescue effect on human breast carcinoma cells is regulated by macrophages

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-02-08 DOI: 10.1016/j.bbrep.2025.101936

Spoorthy Pathikonda , Li Tian , Clement Manohar Arava , Shuk Han Cheng , Yun Wah Lam

{"title":"Radiation-induced rescue effect on human breast carcinoma cells is regulated by macrophages","authors":"Spoorthy Pathikonda , Li Tian , Clement Manohar Arava , Shuk Han Cheng , Yun Wah Lam","doi":"10.1016/j.bbrep.2025.101936","DOIUrl":"10.1016/j.bbrep.2025.101936","url":null,"abstract":"<div><div>The susceptibility of cancer cells to DNA damages is influenced by their microenvironment. For example, unirradiated neighbors of irradiated cells can produce signals that reduce DNA damages. This phenomenon, known as Radiation-Induced Rescue Effect (RIRE), has profound implications on the efficacy of radiotherapy. Using bystander cells co-cultured with mock-irradiated cells as a control, we demonstrated, for the first time, two types of RIRE. Conditioned medium from naïve by stander cells, i.e., cells not exposed to irradiated cells, could mitigate UV-induced DNA damages in human breast carcinoma MCF7 cells, as judged by phospho-H2AX and 53BP1 immunostaining. This protective effect could be further enhanced by the prior treatment of bystander cells with factors from UV-irradiated cells. We named the former effect “basal RIRE” and the latter “active RIRE” which were cell type-dependent. As bystanders, MCF7 showed a significant active RIRE, whereas THP1-derived macrophages showed a strong basal RIRE but no active RIRE. Interestingly, RIRE of macrophages could further be modulated by polarisation. The basal RIRE of macrophages was abolished by M1 polarisation, while M2 and Tumour Associated Macrophages (TAM) demonstrated pronounced basal and active RIRE. When mixtures of MCF7 cells and polarised macrophages were used as bystanders, the overall RIRE was dictated by macrophage phenotypes: RIRE was suppressed by M1 macrophages but significantly enhanced by M2 and TAM. This study shows a previously unappreciated role of the innate immune system in RIRE. Depending on polarised phenotypes, macrophages in the tumour microenvironment can interfere with the effectiveness of radiotherapy by adjusting the RIRE magnitudes.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101936"},"PeriodicalIF":2.3,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of differential mRNA and circRNA profiles in primary and metastatic pancreatic neuroendocrine tumors

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-02-08 DOI: 10.1016/j.bbrep.2025.101935

Gang Li , Jing Zhang , Bentuo Zhang , Dan Wang , Zequn Wang , Yan Pan , Lijie Ma

{"title":"Comprehensive analysis of differential mRNA and circRNA profiles in primary and metastatic pancreatic neuroendocrine tumors","authors":"Gang Li , Jing Zhang , Bentuo Zhang , Dan Wang , Zequn Wang , Yan Pan , Lijie Ma","doi":"10.1016/j.bbrep.2025.101935","DOIUrl":"10.1016/j.bbrep.2025.101935","url":null,"abstract":"<div><div>The diagnosis of primary pancreatic neuroendocrine tumors (pNETs) presents significant challenges, and metastatic pancreatic neuroendocrine tumors are associated with high mortality. Understanding the characteristics of these tumors, particularly the key molecules involved in metastasis, is essential. To address this, we utilized mRNA expression data from human pNET and metastatic pancreatic tumor tissues available in the GEO database and integrated this data with bioinformatics analyses. And then we collected clinical primary tumor and liver metastasis samples from patients with pNETs, we conducted a comprehensive analysis of circular RNAs (circRNAs) to identify key circRNAs associated with the onset and metastasis of pNETs. We found that in pNET development and metastasis, 11 genes and 14 circRNAs were notably upregulated, while 25 genes and 35 circRNAs were significantly downregulated, compared to nearby non-cancerous tissue. Our analysis of differentially expressed RNA and circRNA genes revealed that tumor cell adhesion and integrin activation, regulated by genes like PIEZO1, IFT74, SKAP1, GPX1, F7, VTN, and OMG, are strongly linked to pNET metastasis. We found that SKAP1 levels are positively associated with tumor progression in pNET patients. Overall, our research indicates that the SKAP1-mediated pathway is crucial in pNET development and metastasis.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101935"},"PeriodicalIF":2.3,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corallocarpus glomeruliflorus: Pharmacological potential revealed by phytochemical and in silico investigations

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-02-07 DOI: 10.1016/j.bbrep.2025.101940

Fatima Saleh Naji Bin-Asal , Adel A.M. Saeed , Abdul-Rahman Alawi Bin Yahia

{"title":"Corallocarpus glomeruliflorus: Pharmacological potential revealed by phytochemical and in silico investigations","authors":"Fatima Saleh Naji Bin-Asal , Adel A.M. Saeed , Abdul-Rahman Alawi Bin Yahia","doi":"10.1016/j.bbrep.2025.101940","DOIUrl":"10.1016/j.bbrep.2025.101940","url":null,"abstract":"<div><div><em>Corallocarpus glomeruliflorus</em> (CGP), a plant native to Yemen, has been traditionally used for the management of various health conditions, including cancer, inflammation, and diabetes. This study presents the first comprehensive phytochemical and pharmacological investigation of CGP, revealing novel molecular mechanisms and therapeutic potential. Phytochemical analysis of the CGP extract revealed the presence of diverse bioactive compounds, including phenols, flavonoids, and other secondary metabolites. Notably, this is the first report identifying maritimetin, assafoetidin, kaempferol 3-rhamnoside 7-xyloside, and lespenefril in CGP, compounds with significant therapeutic potential. The total phenolic content was 88.12 ± 4.48 mg GAE/g, significantly higher than previously reported for related species (63.78 ± 1.27 mg GAE/g), and the total flavonoid content was 22.1 ± 0.01 mg QE/g. The extract demonstrated superior antimicrobial activity against Pseudomonas aeruginosa compared to previous studies, with a zone of inhibition of 16.7 ± 1.53 mm at 200 mg/mL concentration. The CGP extract displayed strong antioxidant activity in DPPH, FRAP, and phosphomolybdenum assays, with an IC50 value of 48.39 ± 1.58 μg/mL in the DPPH assay, compared to 9.88 ± 0.54 μg/mL for the positive control ascorbic acid. Most significantly, the CGP extract exhibited more potent selective anticancer effects on human breast (MCF-7) and colon (HCT-116) cancer cell lines than previously reported for related Cucurbitaceae species, with IC50 values of 49.18 ± 2.81 μg/mL and 244.2 ± 9.86 μg/mL, respectively. Our novel molecular docking studies revealed previously unreported interactions between CGP compounds and key therapeutic targets, particularly Pim-1, PIK3CA, α-amylase, and Gyr-B, providing new insights into its mechanism of action.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101940"},"PeriodicalIF":2.3,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143276343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoptosis-inducing proteins with reduced expression in breast cancer: A review article

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-02-05 DOI: 10.1016/j.bbrep.2025.101931

Mohammad Mehdi Khaleghi , Faezeh Rouhi , Kourosh Eslami , Fatemeh Shafiee

引用次数: 0

Resiquimod induces a mixed Th1 and Th2 response via STAT1 and STAT3 signalling in chickens

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-02-04 DOI: 10.1016/j.bbrep.2025.101941

Deepthi Kappala , Saravanan Ramakrishnan , Patel Nikunjkumar Prakashbhai , Abinaya Kaliappan , Prasad Thomas , Jörg Linde , Mithilesh Singh , Sohini Dey , Madhan Mohan Chellappa

{"title":"Resiquimod induces a mixed Th1 and Th2 response via STAT1 and STAT3 signalling in chickens","authors":"Deepthi Kappala , Saravanan Ramakrishnan , Patel Nikunjkumar Prakashbhai , Abinaya Kaliappan , Prasad Thomas , Jörg Linde , Mithilesh Singh , Sohini Dey , Madhan Mohan Chellappa","doi":"10.1016/j.bbrep.2025.101941","DOIUrl":"10.1016/j.bbrep.2025.101941","url":null,"abstract":"<div><div>Resiquimod (R-848), a synthetic TLR7 agonist, modulates immune responses, primarily inducing Th1-biased immunity in mammals. In contrast, our previous studies revealed that R-848 stimulates both Th1 and Th2 responses in chickens. The current research investigates the molecular mechanisms underlying these immune responses in chickens. Pooled splenocytes harvested from chickens (n = 2/group) at 24 h post R-848 treatment were subjected to RNA sequencing and significant differentially expressed genes (DEGs) were identified compared to controls. Eight key genes associated with signal transduction (MAPK14, MAP3K8, PIK3CD, STAT1, STAT3, MAPK11, LRRK2, and GATA3) were validated via real-time PCR in chicken peripheral blood mononuclear cells (PBMCs) from six biological replicates. Pharmacological inhibitors or chloroquine were employed to elucidate the signalling pathways. SB202190, IC-87114, and fludarabine phosphate suppressed the R-848-induced expression of MAPK14, PIK3CD, and STAT1, respectively, while chloroquine decreased STAT3 expression. Intriguingly, chloroquine treatment enhanced the R-848-mediated expression of MAPK11, LRRK2, and GATA3. These results align with transcriptomic findings and highlight the upregulation of STAT1 and STAT3 as potential contributors to the induction of Th1 and Th2 immune responses by R-848 in chickens. These insights provide a foundation for optimizing R-848 as an immunomodulatory agent in avian species.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101941"},"PeriodicalIF":2.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of a high-fat diet and iron overload on erythropoiesis in mice

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-02-01 DOI: 10.1016/j.bbrep.2025.101919

Joe Varghese, Jithu James Varghese, Molly Jacob

{"title":"Effect of a high-fat diet and iron overload on erythropoiesis in mice","authors":"Joe Varghese, Jithu James Varghese, Molly Jacob","doi":"10.1016/j.bbrep.2025.101919","DOIUrl":"10.1016/j.bbrep.2025.101919","url":null,"abstract":"<div><h3>Background</h3><div>Insulin and iron availability stimulate and regulate erythropoiesis, respectively. The effects of hyperinsulinemia and/or iron overload on erythroid differentiation are unclear.</div></div><div><h3>Methodology</h3><div>Male C57Bl/6J wild-type (WT) mice were fed a high-fat diet (HFD) (to produce hyperinsulinemia) or a control diet (CD) for varying periods (4–24 weeks). Hepcidin knock-out (<em>Hamp1</em><sup><em>−/−</em></sup>) mice (which are iron-overloaded) were fed CD or HFD for 24 weeks. Terminal erythroid differentiation (TED) in the bone marrow (BM) from these mice was analyzed by flow cytometry. Hematological parameters were estimated in peripheral blood.</div></div><div><h3>Results</h3><div>HFD-feeding of WT mice did not significantly affect erythroid precursors in the BM or hematological parameters. However, these mice had a significantly higher reticulocyte population in the BM than those fed CD (at all time points studied). Values of hematological parameters were higher in <em>Hamp1</em><sup><em>−/−</em></sup> mice than WT mice, at 24 weeks of feeding (irrespective of diet type), indicating increased erythropoiesis. Early erythroid precursors in the BM were higher in HFD-fed <em>Hamp1</em><sup><em>−/−</em></sup> mice than those fed CD.</div></div><div><h3>Conclusions</h3><div>HFD-feeding in WT mice resulted in increases in the proportion of reticulocytes in the bone marrow; maturation of the early erythroid precursors was not significantly affected. In <em>Hamp1</em><sup><em>−/−</em></sup> mice, HFD-feeding increased the number of early erythroid precursors.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101919"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Identification of hub genes, non-coding RNAs and pathways in Renal cell carcinoma (RCC): A comprehensive in silico study

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-02-01 DOI: 10.1016/j.bbrep.2025.101942

Ahmad Golestanifar , Hengameh Khedri , Parisa Noorabadi , Mohammadreza Saberiyan

{"title":"Identification of hub genes, non-coding RNAs and pathways in Renal cell carcinoma (RCC): A comprehensive in silico study","authors":"Ahmad Golestanifar , Hengameh Khedri , Parisa Noorabadi , Mohammadreza Saberiyan","doi":"10.1016/j.bbrep.2025.101942","DOIUrl":"10.1016/j.bbrep.2025.101942","url":null,"abstract":"<div><h3>Backgrounds</h3><div>Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults. RCC begins in the renal tubule epithelial cells, essential for blood filtration and urine production.</div></div><div><h3>Methods</h3><div>In this study, we aim to uncover the molecular mechanisms underlying kidney renal clear cell carcinoma (KIRC) by analyzing various non-coding RNAs (ncRNAs) and protein-coding genes involved in the disease. Using high-throughput sequencing datasets from the Gene Expression Omnibus (GEO), we identified differentially expressed mRNAs (DEMs), miRNAs (DEMIs), and circRNAs (DECs) in KIRC samples compared to normal kidney tissues. Our approach combined differential expression analysis, functional enrichment through Gene Ontology (GO) and KEGG pathway mapping, and a Protein-Protein Interaction (PPI) network to identify crucial hub genes in KIRC progression.</div></div><div><h3>Results</h3><div>Key findings include the identification of hub genes such as EGFR, FN1, IL6, and ITGAM, which were closely associated with immune responses, cell signaling, and metabolic dysregulation in KIRC. Further analysis indicated that these genes could be potential biomarkers for prognosis and therapeutic targets. We constructed a competitive endogenous RNA (ceRNA) network involving lncRNAs, circRNAs, and miRNAs, suggesting complex regulatory interactions that drive KIRC pathogenesis.</div><div>Additionally, the study examined drug sensitivity associated with the expression of hub genes, revealing the potential for personalized treatments. Immune cell infiltration patterns showed significant correlations with hub gene expression, highlighting the importance of immune modulation in KIRC.</div></div><div><h3>Conclusion</h3><div>This research provides a foundation for developing targeted therapies and diagnostic biomarkers for KIRC while underscoring the need for experimental validation to confirm these bioinformatics insights.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101942"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling ferroptosis genes and inhibitors in diabetic retinopathy through single-cell analysis and docking simulations

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-01-31 DOI: 10.1016/j.bbrep.2025.101932

Md. Maqsood Ahamad Khan , Ananya Ganguly , Shubhrajit Barman , Chirasmita Das , Senthil Kumar Ganesan

{"title":"Unveiling ferroptosis genes and inhibitors in diabetic retinopathy through single-cell analysis and docking simulations","authors":"Md. Maqsood Ahamad Khan , Ananya Ganguly , Shubhrajit Barman , Chirasmita Das , Senthil Kumar Ganesan","doi":"10.1016/j.bbrep.2025.101932","DOIUrl":"10.1016/j.bbrep.2025.101932","url":null,"abstract":"<div><div>Diabetic retinopathy (DR) is a common microvascular complication of diabetes and a leading cause of vision loss worldwide. Although several mechanisms have been implicated in the pathogenesis of DR, emerging evidence suggests a link between ferroptosis and DR. Unfortunately, the exact mechanism underlying this connection is not clear. Therefore, investigating the role of ferroptosis in diabetic retinopathy holds promise for advancing our understanding of this complex disease and developing innovative treatments. We have identified differentially expressed genes (DEGs) and differentially expressed marker genes (DEMGs) from open-source single-cell RNA sequencing datasets by using in depth in silico approach. Subsequently, ferroptosis-associated DEGs (FA-DEGs), ferroptosis-associated DEMGs (FA-DEMGs), and ferroptosis-associated Hub Genes (FAHGs) were identified. The FDA-approved drugs for our target proteins were also identified, and their ADMET properties were assessed. Molecular docking and simulation were utilized to explore the interaction stability of the compounds with the target proteins. Overall, we identified 63 FA-DEMGs that were significantly enriched in Peroxiredoxin activity, Ferroptosis, Mitophagy, and Autophagy. Further analysis predicted that PRDX1 and UBC are candidate target proteins. Molecular docking results showed that dexamethasone has a high binding affinity for both PRDX1 and UBC. Additionally, molecular dynamics simulations revealed that dexamethasone (which showed the best hit in the docking analysis) exhibited a ‘stable effect’ on both PRDX1 and UBC. To summarize, this study showed that PRDX1 and UBC could be suitable therapeutic targets for dexamethasone, which might be helpful in the advance of DR treatments in the future.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101932"},"PeriodicalIF":2.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of epigallocatechin-3-gallate (EGCG) on cognitive functioning and the expression of APP and BDNF in the hippocampus of rats with streptozotocin -induced Alzheimer-like disease

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-01-31 DOI: 10.1016/j.bbrep.2025.101930

Farnaz Ghayour Babaei , Ehsan Saburi , Fatemeh Forouzanfar , Mohadese Asgari , Zakieh Keshavarzi , Vahid Hajali

{"title":"Effect of epigallocatechin-3-gallate (EGCG) on cognitive functioning and the expression of APP and BDNF in the hippocampus of rats with streptozotocin -induced Alzheimer-like disease","authors":"Farnaz Ghayour Babaei , Ehsan Saburi , Fatemeh Forouzanfar , Mohadese Asgari , Zakieh Keshavarzi , Vahid Hajali","doi":"10.1016/j.bbrep.2025.101930","DOIUrl":"10.1016/j.bbrep.2025.101930","url":null,"abstract":"<div><div>We aimed to investigate the potential therapeutic effects of the active substance of green tea, epigallocatechin-3-gallate (EGCG), on behavioral phenotypes and markers of neurogenesis in an Alzheimer disease (AD) rat model. The groups included sham, AD, and three AD groups receiving orally EGCG with different doses of 25, 50, and 100 mg/kg. The AD model was induced by intracerebroventricular (icv) injection of streptozocin (STZ) at a dose of 3 mg/kg. Spatial learning and memory were evaluated in the Morris water maze (MWM) test. Real-time PCR assay was used for evaluating the expression of beta-amyloid precursor protein (APP) and brain-derived neurotrophic factor (BDNF) in the hippocampus of animals. STZ disrupted the function of animals in MWM acquisition phase by almost 65 % and all doses of EGCG could return the learning parameters to those of control animals. STZ also impaired the memory function (P < 0.05) and a dose of 25 mg/kg EGCG could significantly return it to the control level (29 % vs 53 %, P < 0.01). Hippocampal APP gene expression was increased in the AD group and EGCG with dose 25 mg/kg decreased it significantly (P < 0.05). AD animals had decreased levels of hippocampal BDNF and treating with dose 25 mg/kg of EGCG could significantly increase it (P < 0.05). EGCG with dose 25 mg/kg can improve spatial memory deficits in AD model rats. It may be due to the impact on the expression of hippocampal factors involved in AD pathology. These findings could provide a beneficial insight for developing novel, safe, and efficient natural compounds for preventing or alleviation AD symptoms in humans.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101930"},"PeriodicalIF":2.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Ganoderma lucidum polysaccharide attenuates retinal ischemia-reperfusion injury by regulating microglial M1/M2 polarization, suppressing neuroinflammation and inhibiting JAK2/STAT3 pathway

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-01-29 DOI: 10.1016/j.bbrep.2025.101926

Guangyu Zhu , Yujie Liu , Shichun Luo , Chao Tang , Chunlin Zhao , Xuejing Lu

{"title":"Ganoderma lucidum polysaccharide attenuates retinal ischemia-reperfusion injury by regulating microglial M1/M2 polarization, suppressing neuroinflammation and inhibiting JAK2/STAT3 pathway","authors":"Guangyu Zhu , Yujie Liu , Shichun Luo , Chao Tang , Chunlin Zhao , Xuejing Lu","doi":"10.1016/j.bbrep.2025.101926","DOIUrl":"10.1016/j.bbrep.2025.101926","url":null,"abstract":"<div><div>Retinal ischemia-reperfusion (RIR) injury is implicated in the pathogenesis of numerous retinal degenerative disorders, resulting in visual impairment or even blindness in millions of individuals worldwide. In recent years, targeting the suppression of microglia-mediated neuroinflammation has emerged as a principal therapeutic strategy for RIR. Ganoderma lucidum polysaccharide (GLP), a pivotal bioactive extract of Ganoderma lucidum, has been demonstrated to possess efficacious anti-neuroinflammatory properties, while the precise impact of it on RIR injury remains incompletely elucidated. In this study, the RIR model was induced in Sprague-Dawley rats by elevating the intraocular pressure to 80 mmHg for 60 min. Our findings revealed that GLP significantly alleviated inflammatory processes by impeding the secretion of pro-inflammatory cytokines while facilitating that of anti-inflammatory cytokines. Moreover, the administration of GLP also promoted the polarization of microglia from the M1 phenotype to the M2 phenotype. Further investigation of the treatment mechanism indicated that the regulatory effects of GLP were presumably mediated by the inhibition of the JAK2/STAT3 signaling pathway. To summarize, we provided a novel insight into the mechanisms by which GLP ameliorated RIR injury, thereby indicating that it could be identified as a promising candidate for the management of RIR-related diseases.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101926"},"PeriodicalIF":2.3,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0