Biochemistry and Biophysics Reports最新文献

{"title":"Antibacterial efficacy of combined atmospheric cold plasma and hydrogen peroxide treatment on a wound surrogate","authors":"Praj K. Patel ,&nbsp;Preisha Mishra ,&nbsp;Habiba K. Ashour ,&nbsp;Neil R. Mandar ,&nbsp;Safa Mbarki ,&nbsp;Yong Mao ,&nbsp;Suneel Kumar ,&nbsp;Francois Berthiaume ,&nbsp;Aaron D. Mazzeo","doi":"10.1016/j.bbrep.2025.102296","DOIUrl":"10.1016/j.bbrep.2025.102296","url":null,"abstract":"<div><div>This study aims to understand the potential combined effects of treating wound-like tissue surfaces with cold plasma (CP) and hydrogen peroxide. We assess how CP treatment generated by a surface dielectric barrier discharge (SDBD) device achieves bacterial inactivation on two test surfaces: agar plates, representing a surface with uniform topology, and muscle tissue from a thin-sliced chicken breast, representing a non-uniform topology mimicking a wound-like surface. A 10-min CP treatment inactivates <em>Escherichia coli (E. coli)</em> with up to 7 log reduction in colony-forming units (CFU) on a smooth agar surface; however, on chicken breast, the same treatment yields a 0.88 log reduction. By comparison, the common antiseptic H₂O₂ (3 %) yields a 1.06 log CFU reduction on chicken breast after 10 min of treatment. Simultaneous treatment with CP and H₂O₂ increases <em>E. coli</em> inactivation to 1.69 log CFU. Bacterial inactivation is less efficient on the chicken tissue than on smooth agar surfaces. Furthermore, the CP-H₂O₂ combination significantly improves bacterial inactivation, which can be further enhanced by extending treatment time. This work demonstrates an approach to evaluating the efficacy of combining CP with liquid antimicrobial treatments on an accessible wound surrogate with complex morphology and biochemistry. This approach has the potential to serve as a fast method to screen candidate treatments before performing animal studies.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102296"},"PeriodicalIF":2.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased production of humoral factor and PD-L1 expression induced by cytokine stimulation play a key role in the immunosuppressive effects of stem cells derived from human exfoliated deciduous teeth","authors":"Shin Tsunekawa ,&nbsp;Makoto Kato ,&nbsp;Kenta Iwasaki ,&nbsp;Yuko Miwa ,&nbsp;Yusuke Hayashi ,&nbsp;Takako Izumoto ,&nbsp;Akihito Yamamoto ,&nbsp;Tatsuhito Himeno ,&nbsp;Masaki Kondo ,&nbsp;Takaaki Kobayashi ,&nbsp;Hideki Kamiya ,&nbsp;Kohei Ishiyama","doi":"10.1016/j.bbrep.2025.102295","DOIUrl":"10.1016/j.bbrep.2025.102295","url":null,"abstract":"<div><h3>Background</h3><div>Recently, the efficacy of mesenchymal stem cells (MSC) targeting transplant immunosuppression has been reported, and their application to islet transplantation is also expected. However, since the stability of functional manifestation of MSC remains unclear, we have investigated the stability and efficacy of cytokine-stimulated stem cells from human exfoliated deciduous teeth (SHED) in the human immune system in order to establish a safety clinical application.</div></div><div><h3>Methods</h3><div>SHED were stimulated with TNF-α, IL-1β, and IFN-γ, which were elevated post-transplant in the liver. Flow cytometry was used to analyze surface antigen expression. Human peripheral blood mononuclear cells (PBMC) were co-cultured with stimulated SHED directly or indirectly to assess PBMC proliferation. Cytotoxicity assay evaluated PBMC-induced damage to induced pluripotent stem (iPS) cell-derived human pancreatic beta-like cells. ELISA measured immunomodulatory factor secretion.</div></div><div><h3>Results</h3><div>Programmed death-ligand 1 (PD-L1) expression was assessed, and PBMC were co-cultured with stimulated SHED in the presence of anti-PD-L1 antibody. SHED were also reaggregated with dissociated human pancreatic islets to generate islet-like organoids, and insulin secretion was measured. Stimulated SHED showed minimal change in cell surface markers but significantly inhibited PBMC proliferation and cytotoxicity, Stimulated SHED produced elevated levels of immunosuppressive factors and expressed PD-L1. The immunosuppressive effect was partially inhibited by inhibiting cell contact between SHED and PBMC or blocking the PD-1/PD-L1 pathway. Furthermore, insulin secretion was enhanced in reaggregated human pancreatic islets with SHED.</div></div><div><h3>Conclusions</h3><div>We demonstrated that the use of SHED in its activated state effectively suppresses immune response and maintains graft function at the time of islet transplantation.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102295"},"PeriodicalIF":2.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxia response in glioblastoma cells: effect of trehalose on macropinocytosis, autophagy and cell survival","authors":"Barbara Del Bello ,&nbsp;Cristina Ulivieri ,&nbsp;Emilia Maellaro","doi":"10.1016/j.bbrep.2025.102284","DOIUrl":"10.1016/j.bbrep.2025.102284","url":null,"abstract":"<div><div>In glioblastoma multiforme, the most malignant brain tumor in adults, the hypoxic milieu is believed to contribute to tumor aggressiveness and resistance to therapy. Here, human glioblastoma U373-MG and T98G cells were exposed to hypoxia (1 % oxygen) or normoxia (18 % oxygen), and treated with trehalose, a natural disaccharide increasingly studied for its therapeutic potential in cancer. In all samples under hypoxia, HIF-1α stabilization was accompanied by a decrease in Nrf2 and p62/SQSTM1 proteins; redox imbalance also occurred, as documented by increased levels of ROS and parallel lowering of glutathione. Trehalose treatment increased Nrf2 and p62 proteins under normoxia, an effect lost or downsized under hypoxia. Differently, under both oxygen concentrations, trehalose increased glutathione content, consistently with its antioxidant role. In U373-MG cells, trehalose induced remarkable macropinocytosis under hypoxia, albeit less than under normoxia; on the contrary, in autophagy-proficient T98G cells, trehalose further increased the autophagic process under hypoxia compared to normoxia. As regards long-term cell fate (evaluated as colony-forming capacity), hypoxia only proved to be a favorable condition for T98G cells. However, in both cell lines, trehalose treatment significantly and dose-dependently decreased clonogenic capacity under normoxia and hypoxia; in particular, the long-lasting stimulation of macropinocytosis in U373-MG cells induced extensive cell death by methuosis. Overall, our findings suggest that trehalose-induced macropinocytosis or autophagy could also play a tumour-suppressive role in the hypoxic tumor milieu that characterizes glioblastoma, making its synergy with conventional chemotherapy and radiotherapy worth exploring.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102284"},"PeriodicalIF":2.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The antioxidant effect of resveratrol on leukocytes from patients with Alzheimer is independent of SIRT1 signaling pathway","authors":"Filipe Nogueira Franco,&nbsp;Luciana de Cassia Cardoso,&nbsp;Bárbara Néllita Moura Silva,&nbsp;Glaucy Rodrigues de Araújo,&nbsp;Miriam Martins Chaves","doi":"10.1016/j.bbrep.2025.102291","DOIUrl":"10.1016/j.bbrep.2025.102291","url":null,"abstract":"<div><div>Alzheimer's Disease (AD) is the most prevalent dementia in aging. Among its aspects is cognitive and functional decline, resulting from an increase in Reactive Oxygen Species (ROS) and Nitrogen (RNS). Resveratrol (RSV) is a polyphenolic compound that has been recognized as a potent antioxidant. The objective was to verify the oxidative profile of AD in leukocytes, correlating the main oxidative parameters with the functionality of these elderly individuals and verify the antioxidant effect of RSV. For this, ROS and RNS, the antioxidant enzymes catalase and glutathione peroxidase (GPx), as well as the action of the SIRT1 on leukocytes of elderly people without and with AD, in the presence and absence of RSV, were evaluated. It was observed that RSV, despite acting in the AD group, had its antioxidant power reduced compared to the group without AD. RSV was able to increase GPx in both groups. Analyzing SIRT1, we observe the silencing of this signaling pathway in leukocytes from AD. AD was more dependent on the Katz index. Therefore, we observed that oxidative stress predisposes to an increased loss of autonomy and independence in AD and that the antioxidant effect of RSV is reduced.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102291"},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulphide and oleic acid synergism in accelerating mcl-PHA biopolymer production in Pseudomonas aeruginosa MCC 5300 by modulating electron transport system","authors":"Raghavendra Paduvari,&nbsp;Divyashree Somashekara","doi":"10.1016/j.bbrep.2025.102286","DOIUrl":"10.1016/j.bbrep.2025.102286","url":null,"abstract":"<div><div>Environmental concerns raised by petroleum-based plastics have sparked research on eco-friendly biodegradable polymers as alternatives. The medium chain length polyhydroxyalkanoates (mcl-PHA) are one such elastomeric polymer produced by a few bacteria that find various industrial, agricultural and biomedical applications. Besides its vast application, the low yield of wild-type bacterial strains and yield reduction due to reduced growth during prolonged cultivation in nutrient-limiting conditions limit industrial mcl-PHA production. In the present study, <em>Pseudomonas aeruginosa</em> MCC 5300 produced mcl-PHA of about 44.7 % CDW in tryptic soy broth (TSB) media containing oleic acid at 24 h of growth. The sulphide in media enhanced mcl-PHA content up to 86.5 % CDW in TSB media containing oleic acid at 24 h of growth. This is the first report of high mcl-PHA production at a short duration of 24 h in nutrient-enriched conditions. The oleic acid inhibited cytochrome <em>c</em> oxidase activity, shifting the electron flow from ubiquinol to cytochrome <em>c</em> pool. The sulphide increased the expression of bd-oxidase and enhanced electron flux through it, causing a rapid decline in the cellular NADH levels to maintain proton gradient and energy generation. The NADH reduction is compensated by excess mcl-PHA accumulated in bacteria. Hence, mcl-PHA maintains cellular redox-homeostasis during respiration using bd-oxidase.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102286"},"PeriodicalIF":2.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning and WGCNA reveal the PVT1/miR-143–3p/CDK1 ceRNA axis as a key regulator in NSCLC","authors":"Arash Safarzadeh,&nbsp;Setareh Ataei,&nbsp;Arezou Sayad,&nbsp;Soudeh Ghafouri-Fard","doi":"10.1016/j.bbrep.2025.102292","DOIUrl":"10.1016/j.bbrep.2025.102292","url":null,"abstract":"<div><div>Machine learning has provided novel tools for analysis of multi-omics data for subgroups recognition in cancer to reach a clinically meaningful classification of cancer and identification of potential biomarkers. In this work, we retrieved mRNA, lncRNA, miRNA and protein expression data of non-small cell lung cancer (NSCLC) samples and used different machine learning methods for biomarker selection, diagnostic validation, construction of competing endogenous RNA network, identification of the hub axes and drug prediction. Integration of multi-omics data and machine learning resulted in identification of CDK1, TOP2A, AURKA, TPX2, BUB1B, and CENPF as key biomarkers in NSCLC. We also identified the PVT1/miR-143–3p/CDK1 axis and its associated transcription factors (FOXC1, YY1, and GATA2) as a potential regulatory network for additional investigations. These findings increase the understanding of NSCLC molecular processes and provide a foundation for developing targeted therapies and diagnostic tools.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102292"},"PeriodicalIF":2.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-platform urinary metabolomics identifies candidate diagnostic biomarkers in Psoriatic Arthritis","authors":"Koteswari Peddi ,&nbsp;Satyanarayana Swamy Cheekatla ,&nbsp;Usharajeswari Davulury ,&nbsp;Prathyash Ushus Mj ,&nbsp;Satish Mutyam ,&nbsp;Siva Kumar kandula ,&nbsp;Nitalaksheswara Rao Kolukula ,&nbsp;Vishnu Vardhan Reddy Munagala ,&nbsp;Sivakumar Vallabhapurapu","doi":"10.1016/j.bbrep.2025.102289","DOIUrl":"10.1016/j.bbrep.2025.102289","url":null,"abstract":"","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102289"},"PeriodicalIF":2.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saturated fatty acid- and/or monounsaturated fatty acid-containing-phosphatidic acids selectively interact with and activate phosphoglycerate mutase 1","authors":"Kamila Dilimulati ,&nbsp;Naoto Yachida ,&nbsp;Fumi Hoshino,&nbsp;Fumio Sakane","doi":"10.1016/j.bbrep.2025.102285","DOIUrl":"10.1016/j.bbrep.2025.102285","url":null,"abstract":"<div><div>This study aimed to identify the target proteins of 16:0/16:0-phosphatidic acid (PA), which is produced by diacylglycerol kinases (DGKs) α, δ, and ζ. We identified phosphoglycerate mutase 1 (PGAM1), a key glycolytic enzyme that catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate, as a PA-binding protein with a stronger affinity for PA than for other phospholipids, including phosphatidylinositol, phosphatidylinositol 4-monophosphate, phosphatidylinositol 4,5-bisphosphate, cardiolipin, phosphatidylserine, phosphatidylglycerol, phosphatidylcholine, and sphingomyelin. PGAM1 preferentially binds to saturated fatty acid (SFA)- and/or monounsaturated fatty acid (MUFA)-containing PAs, such as 16:0/16:0-, 16:0/18:1-, 18:0/18:0-, 18:0/18:1-, and 18:1/18:1-PA, compared to polyunsaturated fatty acid-containing PAs, such as 18:0/20:4- and 18:0/22:6-PA. Notably, 16:0/16:0- and 16:0/18:1-PA altered the secondary conformation of PGAM1 and substantially enhanced its activity. Interestingly, PGAM1 interacted with DGKδ and ζ, but not with DGKα. These findings indicate that SFA- and/or MUFA-containing-PAs selectively interact with PGAM1, a promising therapeutic target for cancer, type 2 diabetes mellitus, and senescence, to regulate its activity.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102285"},"PeriodicalIF":2.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigating alcohol-induced liver oxidative stress and dysregulated autophagy with protein hydrolysates derived from rainbow trout by-products","authors":"Fahimeh Hasani Zangbar ,&nbsp;Ebrahim Najdegerami ,&nbsp;Mojtaba Hadian ,&nbsp;Ali Shalizar-Jalali","doi":"10.1016/j.bbrep.2025.102238","DOIUrl":"10.1016/j.bbrep.2025.102238","url":null,"abstract":"<div><h3>Background</h3><div>Chronic alcohol consumption causes irreversible liver damage. With 23 % waste from 29 million tons of annual production, rainbow trout is a valuable source of natural antioxidants. This study explores the hepatoprotective effects of rainbow trout protein hydrolysates in an alcohol-induced fatty liver disease (AFLD) rat model, focusing on autophagy, apoptosis, and oxidative stress pathways.</div></div><div><h3>Methods</h3><div>Twenty-four male rats were divided into four groups: Control (C), alcohol (A), protein hydrolysates (P), and alcohol + protein hydrolysates (AP). At the end of the experiment, liver tissue samples were collected for analysis. Oxidative stress markers, including malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH), were assessed. Additionally, the expression of genes related to apoptosis (p53) and autophagy (Beclin1, Atg7, P62) in the liver was evaluated. To validate the gene expression results, the protein expression levels of LC3 and p53 were also measured.</div></div><div><h3>Results</h3><div>Alcohol exposure elevated MDA levels while reducing SOD activity and GSH. Hydrolysate treatment restored antioxidant capacity by enhancing SOD and GSH and lowering MDA. Histology showed hepatic steatosis and reduced glycogen in group A, while groups P and AP exhibited significant improvement (p˂0.05). Additionally, hydrolysates inhibited alcohol-induced P53 upregulation and modulated autophagy-related genes (p˂0.05). Immunohistochemistry showed reduced P53 and increased LC3 in the AP group, indicating a shift from apoptosis to autophagy for cellular homeostasis.</div></div><div><h3>Conclusion</h3><div>These results suggest that protein hydrolysates derived from rainbow trout may have therapeutic potential as a dietary intervention for managing alcohol-induced liver injury, pending further validation.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102238"},"PeriodicalIF":2.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and characterization of a functional benzoylecgonine – bovine serum albumin conjugate for quantification of a humanized monoclonal anti-cocaine antibody","authors":"Rose P. Webster,&nbsp;Terence L. Kirley,&nbsp;Andrew R. Ray,&nbsp;Zhen-Hin Chan,&nbsp;Andrew B. Norman","doi":"10.1016/j.bbrep.2025.102282","DOIUrl":"10.1016/j.bbrep.2025.102282","url":null,"abstract":"<div><div>We have developed a humanized monoclonal anti-cocaine antibody (h2E2), as a potential solution for cocaine use disorder. A key milestone was the development of an assay to quantify this monoclonal antibody (mAb) in animal and human blood. Thus, we synthesized a novel benzoylecgonine-1,4-diaminobutane-BSA (BE-diab BSA) conjugate as an antigen for quantifying h2E2 using ELISA. We report here the method of synthesis of this conjugate, BE-diab BSA, and assessment of its binding to the h2E2 mAb using an ELISA and a fluorescence quenching assay. Compared with four commercial BE-BSA conjugates, BE-diab BSA demonstrated markedly stronger mAb binding in ELISA—three of the commercial conjugates showed less than 10 % relative binding. Fluorescence quenching assays confirmed this binding superiority, with the commercial conjugates showing minimal mAb interaction, while BE-diab BSA induced robust intrinsic fluorescence quenching. SDS-PAGE analyses identified structural differences consistent with binding results between our functional conjugate and the commercial preparations. This functional and reproducible in-house conjugation has been integrated into a GLP-validated ELISA, which is now in use for pharmacokinetic analyses and for qualifying antibody release lots for clinical deployment.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102282"},"PeriodicalIF":2.2,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}