Biochemistry and Biophysics Reports最新文献

{"title":"Induced volatolomics to uncover new enzymatic hallmarks of precancerous lesions: a proof of concept on gastric preneoplasia in mice","authors":"Rony Eid ,&nbsp;Estelle Blochouse ,&nbsp;Alban Giese ,&nbsp;Sébastien Papot ,&nbsp;Philippe Jay ,&nbsp;Christine Varon ,&nbsp;Pauline Poinot","doi":"10.1016/j.bbrep.2025.102062","DOIUrl":"10.1016/j.bbrep.2025.102062","url":null,"abstract":"<div><div>Induced volatolomics is an emerging field of research that offers new opportunities in biology by detecting volatile reporters released by activatable probes, enabling the exploration of oncogenic processes. Building on its proven efficiency in exploring the evolution of implanted tumours, we hypothesized that induced volatolomics could extend its application to detect precancerous conditions. As a proof of concept, we performed a longitudinal study and investigated glycosidase activity during the early stages of gastric carcinogenesis development induced by <em>Helicobacter felis</em> infection in mice, mimicking the gastric carcinogenesis cascade induced by chronic <em>Helicobacter pylori</em> infection in humans. We identified upregulated exoglycosidases linked to acute infections or inflammatory processes in tissues infected by Helicobacter. Specifically, α-mannosidase and β-galactosidase activities in stomach tissue were found to be strongly associated with the initial stages of <em>Helicobacter</em> infection. Additionally, the activities of β-Glucuronidase and β-N-acetyl-glucosaminidase increased during the progression to preneoplastic stages, potentially signalling the transition from infection to inflammation-driven carcinogenesis. These enzymes may serve as early biomarkers for detecting gastric carcinogenesis. Our study highlights the potential of VOC-based probes for real-time monitoring of gastric cancer progression through tissue biopsies. Therefore, this study demonstrates the potential of induced volatolomics for investigating biological processes and uncovering new therapeutic strategies.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102062"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary spectroscopic studies of the interactions between 9-fluoro-5-alkyl-12(H)-quino[3,4-b][1,4]benzothiazine chloride and DNA","authors":"Aleksandra Owczarzy ,&nbsp;Wojciech Rogóż ,&nbsp;Karolina Kulig ,&nbsp;Andrzej Zięba ,&nbsp;Małgorzata Maciążek-Jurczyk","doi":"10.1016/j.bbrep.2025.102067","DOIUrl":"10.1016/j.bbrep.2025.102067","url":null,"abstract":"<div><div>Cancer treatment is one of the challenges of modern medicine. The advancement of new anticancer drugs requires a comprehensive understanding of their mechanism of action and structure-activity relationships. 9-fluoro-5-alkyl-12(H)-quino[3,4-b][1,4]benzothiazine chloride (Salt2) is a newly synthesized substance exhibiting promising anticancer activity. Deoxyribonucleic acid (DNA) is a macromolecule of high biological importance. DNA is essential for cellular development, including DNA replication, transcription and translation. Binding of small molecules to DNA can inhibit or modify cellular DNA function and induces cell death. In turn, it can allow to alleviate or control the disease.</div><div>The objective of the research was to assess the interactions of a newly developed substance (Salt2) and calf thymus DNA (ctDNA) using UV-VIS, spectrofluorescence and circular dichroism spectroscopy. The UV-VIS and fluorescence analysis showed that Salt2 might form a strong, intercalative complex in the ground state with ctDNA.</div><div>Due to the fact that Salt2 changes ctDNA structure it can be assumed, that Salt2 can effect on DNA replication, transcription and translation processes and inhibits or modifies cellular DNA function. The results obtained for the Salt2-ctDNA complex, not only encourage further research, but may also prove useful in determining binding to human cellular DNA.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102067"},"PeriodicalIF":2.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the vestibulo-ocular reflex in head-tilt mutant mice","authors":"Shotaro Harada ,&nbsp;Yoshihisa Koyama ,&nbsp;Takao Imai ,&nbsp;Yasumitsu Takimoto ,&nbsp;Hidenori Inohara ,&nbsp;Shoichi Shimada","doi":"10.1016/j.bbrep.2025.102066","DOIUrl":"10.1016/j.bbrep.2025.102066","url":null,"abstract":"<div><div>The vestibulo-ocular reflex (VOR) is an involuntary reflex essential for maintaining clear vision and balance during head movement. It stabilizes gaze by generating compensatory eye movements in the opposite direction to head movement, ensuring that the visual image is stable on the retina. Maintaining visual stability leads to enhanced postural control and balance, which are crucial for coordinating daily activities, such as exercise and driving. The two types of balance organs, semicircular canals and otolith organs, are involved in the VOR; however, how they interact to contribute to the VOR remains unclear. This study aimed to investigate the interrelationship between semicircular canals and otolith organs in terms of balance function by performing semicircular canal and otolith organ function tests using head-tilt (Het) mutant mice that lack otoconia. Linear VOR results indicated that eye movements induced by linear acceleration stimuli were not observed in Het mutant mice, regardless of the degree of gravitational acceleration. Angular VOR was observed in Het mutant mice at 1 and 2 Hz, but a reduced VOR gain was observed at 0.3 Hz. In wild-type mice, both the linear and angular VOR were normal. The present study demonstrated that in Het mutant mice, the angular VOR decreased after 0.3-Hz semicircular canal stimulation, but normal responses were observed after 1- and 2-Hz semicircular canal stimulation. These results suggest that otolith organs are closely involved in the angular VOR during slow rotational stimulation.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102066"},"PeriodicalIF":2.3,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing carbonate mineral-forming bacterial strains and their carbonic anhydrase activities in two coastal sabkhas","authors":"Khaled Naja ,&nbsp;Sara H. Alhadidi ,&nbsp;Hadil Elsayed ,&nbsp;Jassim Abdulla A. Al-Khayat ,&nbsp;Fadhil Sadooni ,&nbsp;Hamad Al Saad Al-Kuwari ,&nbsp;Zulfa Ali Al Disi","doi":"10.1016/j.bbrep.2025.102064","DOIUrl":"10.1016/j.bbrep.2025.102064","url":null,"abstract":"<div><div>The enzyme carbonic anhydrase (CA) plays a key role in carbonate mineral formation by facilitating the interconversion between CO₂ and bicarbonate ions, thus influencing carbonate precipitation processes in natural environments. This study investigates the biomineralization potential of <em>Virgibacillus</em> strains isolated from two distinct coastal sabkhas in Qatar—Dohat Faishakh Sabkha (DFS) and Khor Al-Adaid Sabkha (KAS)—to better understand the enzymatic mechanisms driving carbonate formation in hypersaline environments. The isolated strains were evaluated for mineral formation and CA activity using three artificial media designed to simulate natural conditions: MD1, seawater-based with tryptone (SWTr), and evaporated seawater-based with tryptone (EWTr). While all strains demonstrated the ability to form minerals in MD1, only <em>Virgibacillus salarius</em> and <em>Virgibacillus marismortui</em>, both exclusive to DFS, exhibited robust mineral precipitation in SWTr and EWTr media. These strains also showed significantly higher CA activity compared to <em>Virgibacillus chiguensis</em> and <em>Virgibacillus dokdonensis</em>, which were present in both sabkhas but displayed limited mineralization and low enzymatic activity under saline conditions.</div><div>Statistical analyses, including ANOVA and principal component analysis (PCA), confirmed the significant role of CA activity and salinity in modulating biomineralization potential among these strains. This research underscores the potential of CA-driven biomineralization for environmental applications. The ability of <em>V. salarius</em> and <em>V. marismortui</em> to precipitate carbonates under high-salinity conditions positions them as promising candidates for bio-based carbon sequestration technologies.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102064"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interplay between tunneling nanotubes and Wnt Signaling: Insights into cytoskeletal regulation and therapeutic potential","authors":"Tengfei Feng ,&nbsp;Qi Xu ,&nbsp;Shuangshuang Wang ,&nbsp;Dongyu Hou ,&nbsp;Xunwei Wu","doi":"10.1016/j.bbrep.2025.102065","DOIUrl":"10.1016/j.bbrep.2025.102065","url":null,"abstract":"<div><div>Tunneling nanotubes (TNTs) are membranous structures that enable direct intercellular transfer of mitochondria, proteins, RNAs, and signaling molecules, playing key roles in tissue repair, immune coordination, and stress adaptation. Among their critical functions, TNT-mediated mitochondrial transfer rescues metabolically impaired cells, yet the regulatory mechanisms governing TNT formation and function remain incompletely understood. Recent studies highlight the Wnt signaling pathway—a conserved regulator of cell fate, polarity, and cytoskeletal remodeling—as a central modulator of TNT dynamics. Through its canonical (Wnt/β-catenin) and non-canonical (Wnt/PCP and Wnt/Ca²⁺) branches, Wnt signaling orchestrates actin filament organization, bundling, and turnover, all of which are essential for TNT biogenesis and stability. This review critically examines the mechanistic intersection between Wnt signaling and TNTs, with an emphasis on how Wnt-driven cytoskeletal remodeling supports intercellular connectivity. Beyond basic mechanistic insights, we also explore the physiological and pathological relevance of this crosstalk—including its roles in tissue regeneration, immune modulation, cancer progression, and neurodegeneration. While the Wnt–TNT axis offers therapeutic promise, its context-dependent effects demand careful consideration.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102065"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive risk model of disulfidoptosis-related lncRNAs predicts prognosis and therapeutic implications in bladder cancer","authors":"Zhixiong Zhang ,&nbsp;Jinghua Zhong ,&nbsp;Muhammad Sarfaraz Iqbal ,&nbsp;Zhiwen Zeng ,&nbsp;Xiaolu Duan","doi":"10.1016/j.bbrep.2025.102060","DOIUrl":"10.1016/j.bbrep.2025.102060","url":null,"abstract":"<div><h3>Background</h3><div>Disulfidoptosis is an emerging form of regulated cell death; however, the roles of its associated long non-coding RNAs (dr-lncRNAs) in bladder cancer (BLCA) remain poorly characterized. By leveraging the most comprehensive curated dataset of disulfidoptosis-related genes to date, we systematically developed and validated a novel dr-lncRNA signature that elucidates the prognostic significance and immune microenvironmental dynamics in BLCA.</div></div><div><h3>Methods</h3><div>The Cancer Genome Atlas (TCGA) database was utilized to extract significant clinical and RNA sequencing data of BLCA patients. Cox and Lasso regression with several variables was used to create a risk model. ROC, Kaplan-Meier, and nomogram analyses were carefully reviewed for validity. The validated study evaluated intricate interactions between functional enrichment, immune cell infiltration, cancer mutation load, and treatment sensitivity. Unsupervised consensus clustering identified subgroup patterns that reflected immune system alterations, medication susceptibility, and prognosis.</div></div><div><h3>Results</h3><div>Nine lncRNAs significantly correlated with prognosis were collectively identified, subsequently forming the basis for constructing a risk model consisting of seven lncRNAs. The model exhibited significant superiority in predicting patient outcomes, effectively distinguishing between high-risk from low-risk individuals. Functional enrichment analysis uncovered their potential involvement in immune-related biological pathways. Patients in the high-risk group exhibited higher tumor mutation burdens, more active immune functions and a higher sensitivity to chemotherapeutic drugs. Variations among BLCA subgroups were identified by consensus cluster analysis, including clinical characteristics, prognosis, lncRNA expression, immune cell infiltration, and immune checkpoint profiles.</div></div><div><h3>Conclusion</h3><div>The dr-lncRNAs-based risk model presents a promising tool for predicting prognosis and guiding personalized immunotherapy and treatment strategies in BLCA patients.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"42 ","pages":"Article 102060"},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spinal cord injury models: Advantages and disadvantages in the view of pathophysiology and clinical significance","authors":"Min-Qi Li ,&nbsp;Qing-Hua Wang ,&nbsp;Chuan-Ming Dong ,&nbsp;Long-Ju Qi","doi":"10.1016/j.bbrep.2025.102063","DOIUrl":"10.1016/j.bbrep.2025.102063","url":null,"abstract":"<div><div>Over recent decades, SCI research has advanced in understanding its pathophysiology and related mechanisms. Researchers developed many rodent SCI models to mimic injury processes, which are crucial for evaluating therapies and understanding pathology. But choosing the right model for specific research is challenging due to distinct pathological changes. This review overviews SCI pathophysiology and examines rodent models. It emphasizes model - related challenges, application value, simulation degrees, and replication of pathologies. It also discusses model advantages and limitations, and references recent reverse-engineering projects, which provide a brand-new perspective for promoting SCI research.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"42 ","pages":"Article 102063"},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144139339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value and immune infiltration analysis of a novel lactylation-related gene signature in endometrial cancer","authors":"Liqin Gu ,&nbsp;Chunnian Zhang ,&nbsp;Minjuan Xu ,&nbsp;Fang Peng ,&nbsp;Ruo-Hui Huang ,&nbsp;Deping Luo","doi":"10.1016/j.bbrep.2025.102056","DOIUrl":"10.1016/j.bbrep.2025.102056","url":null,"abstract":"<div><h3>Background</h3><div>Lactylation has been implicated in tumor growth, proliferation, and metastasis; however, its precise relationship with cancer remains poorly understood. This study aims to elucidate the role of lactylation-related genes (LRGs) in the development of endometrial cancer (EC).</div></div><div><h3>Methods</h3><div>We utilized data from The Cancer Genome Atlas (TCGA) database to analyze the expression and mutation patterns of LRGs in EC. Univariate Cox regression analysis and Lasso-Cox regression analysis were employed to identify prognosis-related genes and construct a risk model. EC samples were stratified into high-risk and low-risk groups based on the risk values derived from the model. These groups were validated using both training and validation cohorts. Additionally, we assessed differences in the immune microenvironment, tumor mutation burden (TMB), and drug response between the high-risk and low-risk groups.</div></div><div><h3>Results</h3><div>Differentially expressed genes (DEGs) between EC and control samples were identified, and their intersection with LRGs yielded differentially expressed lactylation-related genes (DLRGs). A total of six prognostic DLRGs (PFKM, H3C1, SIRT3, VIM, WAS, and LSP1) were selected and used to construct an EC risk model. Significant differences in prognosis, immune microenvironment, TMB, and drug sensitivity were observed between the high-expression and low-expression groups.</div></div><div><h3>Conclusion</h3><div>LRGs play a significant role in endometrial cancer by influencing cell growth, the immune microenvironment, and drug response. The six DLRGs included in the risk model may serve as potential prognostic markers and therapeutic targets for EC.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"42 ","pages":"Article 102056"},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidirectional therapeutic effects of synthesized HMGB1 peptide on liver cirrhosis in mice","authors":"Masaki Mito ,&nbsp;Atsunori Tsuchiya ,&nbsp;Soichi Ishii ,&nbsp;Takafumi Tonouchi ,&nbsp;Kaito Furuyama ,&nbsp;Ryo Jinbo ,&nbsp;Nobutaka Takeda ,&nbsp;Hiroyuki Abe ,&nbsp;Katsuto Tamai ,&nbsp;Shuji Terai","doi":"10.1016/j.bbrep.2025.102061","DOIUrl":"10.1016/j.bbrep.2025.102061","url":null,"abstract":"<div><h3>Aim</h3><div>Liver cirrhosis is a serious disease characterized by liver dysfunction and severe fibrosis; however, no breakthrough drugs have effectively improved fibrosis, making it an unmet medical need. We have previously reported that the HMGB1 peptide, synthesized from box A of the HMGB1 protein, ameliorates liver fibrosis and is a promising candidate for fibrosis-improving drugs against liver cirrhosis. In this study, we used spatial analysis to observe treatment-induced changes over time.</div></div><div><h3>Methods</h3><div>Liver cirrhosis was induced in C57BL/6J mice using carbon tetrachloride (CCl4) injections, followed by HMGB1 peptide treatment. To assess the temporal effects of HMGB1 on the liver in a CCl4-induced cirrhosis mouse model, we used GeoMx spatial analysis. We focused on αSMA-positive active hepatic stellate cells (HSCs), F4/80-positive macrophages, and CK8/18-positive hepatocytes to determine how each cell type was affected over time. Statistical analyses were conducted using GraphPad Prism9, with significance set at p &lt; 0.05.</div></div><div><h3>Results</h3><div>In cirrhotic mice, we first observed a decrease in the number of activated HSCs over time, two weeks after treatment initiation. Macrophage-associated genes ceased to induce fibrosis-related pathways early in the treatment. This suggests that the effect of macrophages on fibrosis was weakened by the treatment. We also confirmed that lipid metabolism of hepatocytes may be improved during treatment. Furthermore, <em>Cxcl12</em> and <em>Ccl25</em> expression were induced in the peptide-treated group, indicating possible cell migration to the liver.</div></div><div><h3>Conclusion</h3><div>Over time, macrophages followed by HSCs, showed the most notable changes with treatment, resulting in improved fibrosis. The HMGB1 peptide drug also affected lipid metabolism in hepatocytes, suggesting a positive therapeutic effect on steatohepatitis. Elevated factors that promote cell migration may have also enhanced the healing effect.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"42 ","pages":"Article 102061"},"PeriodicalIF":2.3,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The miR-146a-associated HDAC2 regulation of PI3K is involved in pancreatitis in vitro","authors":"Ding-wen Zhong ,&nbsp;Xiang-tian Zeng ,&nbsp;Wen-hui Chen ,&nbsp;Xian-yu Huang ,&nbsp;Jia-xin Liu ,&nbsp;Yong-hui Liao","doi":"10.1016/j.bbrep.2025.102057","DOIUrl":"10.1016/j.bbrep.2025.102057","url":null,"abstract":"<div><h3>Purpose</h3><div>To investigate the association between miR-146a/HDAC2 and their regulatory roles on the PI3K expression during pancreatitis.</div></div><div><h3>Methods</h3><div>Rat pancreatic AR42J cells were treated with LPS for simulating pancreatitis. Expression levels of inflammatory factors (IL-6, TNF-α) and miR-146a were d<strong>etected to</strong> determine the optimal LPS concentration for establishing an in vitro pancreatitis model<strong>.</strong> Cell proliferation and apoptosis were analyzed using CCK-8 and flow cytometry<strong>.</strong> Immunofluorescence was performed to assess co-localization of HDAC2 and PI3K. ELISA quantified TNF-α and IL-6 levels in cell supernatants. A dual-luciferase assay verified the targeting relationship between miR-146a and HDAC2.</div></div><div><h3>Results</h3><div>Compared to controls, the cell proliferation ability of the pancreatitis model group was decreased, whereas TSA and miR-146a mimic interventions restored proliferation. The expression of IL-6 and TNF-αin the LPS group was higher than that in the control group, and their expression decreases in the TSA and miR-146a mimic intervention group. Besides the dual luciferase detected the targeting relationship between miR-146a and HDAC2, the immunofluorescence showed co-localization of HDAC2 and PI3K.</div></div><div><h3>Conclusions</h3><div>TSA and miR-146a mimic enhance proliferation and reduce inflammation in pancreatitis cells. The miR-146a/HDAC2 axis may mediate therapeutic effects in pancreatitis by modulating the PI3K expression.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"42 ","pages":"Article 102057"},"PeriodicalIF":2.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}