Biochemistry and Biophysics Reports最新文献

{"title":"Pomegranate root extract possesses anti-metastatic potential by suppressing invasiveness and vasculogenic mimicry capability of cancer cells","authors":"Khajeelak Chiablaem ,&nbsp;Kriengsak Lirdprapamongkol ,&nbsp;Jisnuson Svasti","doi":"10.1016/j.bbrep.2025.102290","DOIUrl":"10.1016/j.bbrep.2025.102290","url":null,"abstract":"<div><div>Cancer metastasis is a serious problem in cancer treatment. Metastasis is driven by cancer invasiveness and facilitated by angiogenesis of endothelial cells. Collective evidence revealed that highly invasive cancer cells possess vasculogenic mimicry (VM) capability by forming non-endothelial capillaries that mimic blood vessels. Moreover, VM also resists anti-angiogenic drugs. Thus, anti-invasion and <em>anti</em>-VM are necessary approaches required for anti-metastasis therapy. Pomegranate plants (<em>Punica granatum</em> L.) have been used in traditional medicines since ancient times. Different parts of the pomegranate tree possess the ability to inhibit cancer cell invasion and migration, but there is no report for pomegranate roots. This study aimed to explore anti-metastatic and <em>anti</em>-VM effects of pomegranate root extract (PR) in a human lung cancer cell line (A549) and a VM-forming human hepatocellular carcinoma cell line (SK-Hep-1). At less cytotoxic concentrations (lower than IC₂₀ values), PR dose-dependently reduced invasion, migration, and MMPs production in both A549 and SK-Hep-1 cell lines. PR also inhibited VM formation in SK-Hep-1 cells. Mechanistic studies revealed that, in A549 cells, PR inhibited hepatocyte growth factor (HGF)-induced activation of MET and its downstream AKT and ERK pathways. During VM formation of SK-Hep-1 cells, PR downregulated AKT and ERK signaling pathways without affecting their upstream activator, FAK phosphorylation. Phytochemical profiling of PR analyzed by LC-MS revealed tannins and ellagic acid derivatives were the major classes of natural products present in PR. This research reveals a novel health benefit of pomegranate roots in cancer metastasis therapy.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102290"},"PeriodicalIF":2.2,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple roles of ANO6 in tumors, molecular mechanism and its potential therapeutic value","authors":"Ao Chen ,&nbsp;Chenyu Yang ,&nbsp;Jin Wang","doi":"10.1016/j.bbrep.2025.102230","DOIUrl":"10.1016/j.bbrep.2025.102230","url":null,"abstract":"<div><div>Anoctamin 6 (ANO6/TMEM16F) is a calcium-activated ion channel and phospholipid disruptor that plays a key role in maintaining cellular homeostasis. This review focuses on the role of ANO6 in various cancers. On one hand, ANO6 is highly expressed in pancreatic cancer, gastric cancer, and glioma, while its expression is downregulated in breast cancer, prostate cancer, cervical cancer, and ovarian cancer. This indicates its functional diversity across different cancer types, reflecting the complex regulatory mechanisms of ANO6 in the tumor microenvironment. In pancreatic cancer, ANO6 is highly expressed, and it promotes pancreatic cancer metastasis through the ERK signaling pathway. In melanoma, ANO6 is closely associated with poor patient prognosis, clinical-pathological characteristics, tumor immunity, and tumor heterogeneity. In breast cancer, ANO6 is a ferroptosis gene associated with prognosis, and its low expression is linked to poor outcomes, making it a key independent predictor of overall survival in breast cancer patients. Additionally, the relationship between ANO6 and immune cells highlights its potential role in cancer immune surveillance and therapy. Finally, we found that ANO6 may regulate immune cell exhaustion in the tumor microenvironment by influencing macrophage polarization and T cell recruitment and activation. This review highlights the diverse roles of ANO6 in different cancers and its potential as a diagnostic and therapeutic tool. Future studies should address the mechanistic details of ANO6 involvement in cancer, validate its clinical utility, and explore its therapeutic potential in combination with existing therapies.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102230"},"PeriodicalIF":2.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic model establishment and immune microenvironment analysis based on transcriptomic data of long-term survivors of pancreatic ductal adenocarcinoma","authors":"Lizhi Lin ,&nbsp;Ragnar Norrsell ,&nbsp;Roland Andersson ,&nbsp;Xian Shen ,&nbsp;Daniel Ansari","doi":"10.1016/j.bbrep.2025.102280","DOIUrl":"10.1016/j.bbrep.2025.102280","url":null,"abstract":"<div><div>Pancreatic cancer continues to be a major cause of cancer deaths worldwide. Characterizing the tumors of long-term survivors (≥5 years survival) would create opportunities in prognostic and therapeutic strategies. In this study, RNA sequencing data was used to identify differentially expressed genes (DEGs) in tumors of long-term survivors (LTS) vs short-term survivors (STS). Using LASSO-Cox regression, 4 prognostic DEGs, along with tumor stage, were utilized to develop a model for identifying high- and low-risk tumors. In Kaplan-Meier survival analysis, the high-risk group had significantly worse prognosis in both the training and validation cohorts. Using KEGG pathway gene signature sets, the high-risk group was found to have amplification of pathways, such as focal adhesion and ECM receptor interaction. The low-risk group, meanwhile, showed upregulation of specific metabolic pathways. Using ESTIMATE analysis, the high-risk group was found to have more stromal cell infiltration. Increased unpolarized macrophages and decreased inflammatory/anti-tumoral macrophages were also found in the high-risk group. Lastly, drug sensitivities were calculated and found to be generally higher in the high-risk group. This study reveals a model for predicting survival and drug sensitivity in pancreatic cancer. Genetic, molecular and tumor microenvironment characteristics of tumors from LTS and STS have been identified, highlighting opportunities for further research.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102280"},"PeriodicalIF":2.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated multi-omics analysis reveals TMEM147 as an immunosuppressive prognostic biomarker in LUAD","authors":"Lei Ding ,&nbsp;Yi Ding","doi":"10.1016/j.bbrep.2025.102281","DOIUrl":"10.1016/j.bbrep.2025.102281","url":null,"abstract":"<div><div>TMEM147, an endoplasmic reticulum (ER) membrane protein, is implicated in lung adenocarcinoma (LUAD) progression, although its precise role remains unclear. To elucidate its function, this study integrated bioinformatics analyses with experimental validation. First, TMEM147 expression was assessed using TCGA and GEO datasets, with validation performed in LUAD cell lines. Survival analysis evaluated its prognostic significance. Subsequently, Gene Ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses and single-sample gene set enrichment analysis (ssGSEA) were employed to identify associated functional pathways and interactions within the tumor immune microenvironment. Transcription factor binding predictions and in vitro functional assays (migration, invasion, proliferation) further characterized TMEM147's role. Results indicated that TMEM147 was significantly upregulated in LUAD and correlated with poor outcomes in patients. FLI1 was predicted as a key transcriptional regulator of TMEM147. Furthermore, TMEM147 expression influenced immune cell infiltration profiles and was associated with pathways involved in ribonucleoprotein biogenesis and oxidative phosphorylation (OXPHOS). Importantly, silencing TMEM147 significantly reduced cancer cell migration, invasion, and proliferation. These findings collectively suggest that TMEM147 promotes LUAD progression and holds potential as both a prognostic biomarker and a therapeutic target.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102281"},"PeriodicalIF":2.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Duchesnea indica (Andr.) Focke extracts exerted anti-inflammatory effect via inhibiting MAPK/ERK pathway in LPS-stimulated RAW264.7 cells","authors":"Haipeng Feng ,&nbsp;Qianqian Qiao ,&nbsp;Jingyan Zhang,&nbsp;Kang Zhang,&nbsp;Lei Wang,&nbsp;Jianxi Li","doi":"10.1016/j.bbrep.2025.102273","DOIUrl":"10.1016/j.bbrep.2025.102273","url":null,"abstract":"<div><div><em>Duchesnea indica</em> (Andr.) Focke (<strong>DIF</strong>), a medicinal plant from the Rosaceae family, possesses therapeutic properties such as heat-clearing and detoxification, dispersion of stasis detumescence, blood-cooling, and hemostatic effect. The latest research unveiled a diverse array of pharmacologically active compounds from DIF, showcasing a broad spectrum of pharmacological effects. However, there was limited attention given to the comprehensive investigation of its high molecular weight compounds, particularly polysaccharides. In this study, DIF extracts were prepared by water-extraction and alcohol-precipitation method and the pharmacological effect was detected. The results demonstrated that the total polysaccharide content, reducing sugar content, total flavonoid content, and total polyphenol content in DIF extracts were 32.62 ± 0.91 %, 13.41 ± 0.18 %, 1.07 ± 0.07 %, and 12.16 ± 0.27 %, respectively. Although the total antioxidant activity of DIF extracts were significantly low than that of the vitamin C group, its ability to scavenge ABTS, DPPH, and superoxide anion radicals was similar to that of the vitamin C group. Furthermore, DIF extracts demonstrated significant inhibition on NO and MDA levels while simultaneously enhancing SOD activity in LPS-stimulated RAW264.7 cells. Finally, DIF extracts significantly reduced the mRNA level of pro-inflammation cytokines interleukin (<strong>IL</strong>)-6, IL-1β, inducible nitric oxide synthase (<strong>iNOS</strong>), cyclooxygenase-2 (<strong>COX-2</strong>) and tumor necrosis factor (<strong>TNF</strong>)<strong>-α</strong>, and directly inhibited the phosphorylation level of extracellular regulated kinase and mitogen-activated protein kinase (<strong>ERK-MAPK</strong>) pathway. Taken together, these results indicated that DIF extracts exhibited an anti-inflammatory and antioxidant effect in LPS-induced RAW264.7 mouse macrophages by directly inhibiting ERK-MAPK signaling pathway. Based on these findings, DIF extracts provided new insights into the treatment and prevention of diseases related to oxidative stress and inflammation.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102273"},"PeriodicalIF":2.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the ABTS-based antioxidant potential and antiproliferative activity of pulp, skin and seeds extracts from Nephelium lappaceum, Manilkara zapota, and Meliccocus oliviformis on human prostate and colon cancer cells","authors":"Adelma Escobar-Ramírez ,&nbsp;José A. González Garrido ,&nbsp;Oswaldo Hernández Abreu ,&nbsp;Edgar Zenteno ,&nbsp;Carlos J. Solórzano Mata ,&nbsp;Yobana Pérez Cervera ,&nbsp;Tony Lefebvre ,&nbsp;Vanessa Dehennaut","doi":"10.1016/j.bbrep.2025.102257","DOIUrl":"10.1016/j.bbrep.2025.102257","url":null,"abstract":"<div><div>Despite significant advancements in cancer treatments, cancer remains the second leading cause of death worldwide, creating a pressing need for effective and non-toxic therapies. Plant bioactive compounds, particularly phenolic acids, have shown promising antioxidant and anticancer properties. In this study, we investigated the antioxidant potential and antiproliferative activity of methanolic extracts obtained from the skin, pulp, and seeds of three exotic fruits commonly consumed in Mexico: <em>Manilkara zapota</em>, <em>Nephelium lappaceum</em>, and <em>Meliccocus oliviformis</em>. The antioxidant activity was assessed using the ABTS radical scavenging assay, and total phenolic content was quantified by a modified Folin-Ciocalteu method. Most extracts exhibited significant ABTS radical scavenging capacity, with the highest phenolic content detected in the skin and seeds of <em>N. lappaceum</em>.</div><div>and <em>M. oliviformis</em>. Cytotoxic and antiproliferative effects were evaluated on human colon (HCT116) and prostate (DU145) cancer cell lines. The skin of <em>N. lappaceum</em> and the seeds of <em>M. zapota</em> and <em>M. oliviformis</em> showed strong antiproliferative activity. These findings highlight the therapeutic potential of underutilized fruit by-products and support further investigation into their bioactive constituents as complementary candidates for cancer treatment.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102257"},"PeriodicalIF":2.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of long intergenic non-protein coding RNA 312 in cancer: A bioinformatics and literature based study","authors":"Arash Safarzadeh,&nbsp;Setareh Ataei,&nbsp;Soudeh Ghafouri-Fard","doi":"10.1016/j.bbrep.2025.102283","DOIUrl":"10.1016/j.bbrep.2025.102283","url":null,"abstract":"<div><div>LINC00312 encodes an intronless transcript mainly functioning as a tumor suppressor. Expression of this transcript is downregulated in a variety of cancers, particularly lung cancers. It is regarded as a negative regulator of estrogen receptor signaling. Moreover, low expression of LINC00312 correlates with poor survival in sarcoma and stomach adenocarcinoma, suggesting its potential as a prognostic biomarker. In the current study, we performed a bioinformatics and literature based approach to find the importance of this long non-coding RNA in different cancers, its regulatory network and its interactions with different biomolecules and compounds. Taken together, LINC00312 represents a possible candidate for additional diagnostics and prognostics approaches.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102283"},"PeriodicalIF":2.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HOXA7 can be used for prognostic evaluation and molecular targeted therapy of gliomas: a multidimensional study based on a large sample size","authors":"Zhi Sha ,&nbsp;Zhendong Liu ,&nbsp;Yanzheng Gao","doi":"10.1016/j.bbrep.2025.102279","DOIUrl":"10.1016/j.bbrep.2025.102279","url":null,"abstract":"<div><div><em>HOXA7</em> has been reported to play an important role in various malignant tumors; however, its role in gliomas has not yet been elucidated. This retrospective study aimed to comprehensively investigate the expression patterns, clinical significance, and potential molecular mechanisms of HOXA7 in gliomas through multi-dimensional analysis. Based on a large sample size of 1946 gliomas from various databases, this study used a variety of statistical and bioinformatics methods to explore the role of <em>HOXA7</em> in gliomas at multiple levels (mRNA, cell signaling pathways, clinical characteristics, and prognosis). <em>HOXA7</em> has abnormally high expression in gliomas, and its expression level can be used as an independent prognostic factor for gliomas, as well as having some clinical diagnostic value. In addition, <em>HOXA7</em> may be involved in the regulation of some cancer-related cell signaling pathways, such as pantothenate and CoA biosynthesis. Abnormally high expression of <em>HOXA7</em> may be significantly correlated with multiple clinical features and cancer-related cell signaling pathways, and it may be used as a biomarker and molecular target for evaluating prognosis of gliomas.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102279"},"PeriodicalIF":2.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Usefulness of plasma and apolipoprotein B-depleted serum samples in paraoxonase 1 assessment","authors":"Rina Kawaguchi ,&nbsp;Akira Yoshimoto ,&nbsp;Takahiro Kameda ,&nbsp;Ryunosuke Ohkawa","doi":"10.1016/j.bbrep.2025.102274","DOIUrl":"10.1016/j.bbrep.2025.102274","url":null,"abstract":"<div><div>Paraoxonase 1 (PON1) is closely associated with antioxidant, anti-inflammatory, and antiatherosclerotic functions of high-density lipoprotein (HDL). Although many clinical studies have evaluated relationships between PON1 activity and various diseases based on its multiple functions, their results were contradictory because of the difference of sample preparation methods. Therefore, we investigated an optimal preanalytical method for PON1 analysis by measuring three different PON1 activities in various types of specimens. Samples were prepared from healthy human serum, plasma with or without calcium addition, HDL isolated by ultracentrifugation, and apolipoprotein B-depleted serum (BDS). Using these samples, PON1 protein concentration and activities using three substrate types (<em>p</em>-nitrophenyl acetate, paraoxon, and γ-thiobutyrolactone) were evaluated. PON1 distributions in HDL subfractions from serum and BDS were also investigated. Although PON1 activities in plasma were lower than those in serum, removing EDTA and adding calcium rescued PON1 activities in plasma similar to levels comparable to those in serum. In contrast, HDL isolated by ultracentrifugation had significantly lower PON1 activities and protein concentrations, indicating that many PON1 proteins were not bound to the HDL particle in the HDL fractions collected from serum and plasma by ultracentrifugation. PON1 protein concentration and distributions in BDS showed similar to those in serum sample than those in HDL sample. Furthermore, three types of PON1 activities were differentially affected by sample preparation procedures. The reduction of PON1 activity in BDS differed among individuals and by the activity type. Focusing on each of three different PON1 activities might further enhance the clinical significance of PON1 testing.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102274"},"PeriodicalIF":2.2,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced HBsAg positivity in systemic lupus erythematosus and Sjögren's syndrome: A retrospective comparative study","authors":"Man Li ,&nbsp;Qihang Zhu ,&nbsp;Zhong Fang ,&nbsp;Taihong Huang ,&nbsp;Lei Dai ,&nbsp;Sen Wang","doi":"10.1016/j.bbrep.2025.102275","DOIUrl":"10.1016/j.bbrep.2025.102275","url":null,"abstract":"<div><h3>Background</h3><div>Systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS) are two common systemic autoimmune diseases. Recent studies have suggested that the prevalence of hepatitis B surface antigen (HBsAg) may be lower in patients with SLE compared to the general population; however, the existing evidence remains limited and controversial. A systematic analysis of HBV infection rates and serological marker distributions in patients with SLE and SS may offer important insights into the interaction between autoimmune and antiviral immunity.</div></div><div><h3>Methods</h3><div>We retrospectively analyzed 2421 hospitalized patients with SLE and 2049 with SS at Nanjing Drum Tower Hospital from January 2019 to December 2024. Patients with other autoimmune diseases (including rheumatoid arthritis (RA), ankylosing spondylitis (AS), ulcerative colitis (UC), and Crohn's disease (CD)) and 5927 non-autoimmune hospitalized patients were included as controls. Hepatitis B virus (HBV) serological markers (HBsAg, HBsAb, HBeAg, HBeAb, and HBcAb) and anti-HCV antibodies were tested. We compared the positivity rates of HBV seromarkers across different groups and performed subgroup analyses based on sex, age, and autoantibody profiles.</div></div><div><h3>Results</h3><div>The HBsAg positivity rate was significantly lower in SLE and SS patients compared with non-autoimmune controls (1.2 % vs. 5.2 %, P &lt; 0.0001; 1.7 % vs. 5.2 %, P &lt; 0.0001, respectively). No significant differences were observed in patients with RA, AS, UC, or CD. Stratified analyses revealed consistently lower HBsAg positivity in SLE and SS patients across both sexes and all age groups above 20 years. Further analyses indicated that this phenomenon was not attributable to occult HBV infection, inpatient versus outpatient status, or immunosuppressant use. Notably, high ANA titers and the presence of antibodies such as anti-dsDNA, anti-SSA, and anti-ribosomal P were associated with lower HBsAg positivity. No significant differences in HBsAg positivity were found between patients with or without common complications.</div></div><div><h3>Conclusion</h3><div>SLE and SS patients exhibit consistently lower HBsAg positivity rates, independent of clinical factors, and linked to autoantibody status. These findings suggest disease-specific immune mechanisms that may promote HBV clearance or reduce susceptibility to infection.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102275"},"PeriodicalIF":2.2,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}