印度杜鹃（Andr.）福克提取物通过抑制lps刺激RAW264.7细胞的MAPK/ERK通路发挥抗炎作用

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-09-25 DOI:10.1016/j.bbrep.2025.102273

Haipeng Feng , Qianqian Qiao , Jingyan Zhang, Kang Zhang, Lei Wang, Jianxi Li

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引用次数: 0

摘要

印度杜鹃（Andr.）福克是蔷薇科药用植物，具有清热解毒、散瘀消肿、凉血止血等治疗作用。最新的研究揭示了来自DIF的多种药理活性化合物，展示了广泛的药理作用。然而，对其高分子量化合物，特别是多糖的综合研究却很少。本研究采用水提醇沉法制备DIF提取物，并检测其药理作用。结果表明，DIF提取物的总多糖含量为32.62±0.91%，还原糖含量为13.41±0.18%，总黄酮含量为1.07±0.07%，总多酚含量为12.16±0.27%。虽然DIF提取物的总抗氧化活性明显低于维生素C组，但其清除ABTS、DPPH和超氧阴离子自由基的能力与维生素C组相近。此外，DIF提取物对lps刺激的RAW264.7细胞的NO和MDA水平有明显的抑制作用，同时提高了SOD活性。最后，DIF提取物显著降低促炎细胞因子白介素(IL)-6、IL-1β、诱导型一氧化氮合酶（iNOS）、环氧化酶-2 （COX-2）和肿瘤坏死因子(TNF)-α mRNA水平，并直接抑制细胞外调节激酶和丝裂原活化蛋白激酶（ERK-MAPK）通路磷酸化水平。综上所述，DIF提取物通过直接抑制ERK-MAPK信号通路，对lps诱导的RAW264.7小鼠巨噬细胞具有抗炎和抗氧化作用。基于这些发现，DIF提取物为氧化应激和炎症相关疾病的治疗和预防提供了新的见解。

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Duchesnea indica (Andr.) Focke extracts exerted anti-inflammatory effect via inhibiting MAPK/ERK pathway in LPS-stimulated RAW264.7 cells

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Duchesnea indica (Andr.) Focke extracts exerted anti-inflammatory effect via inhibiting MAPK/ERK pathway in LPS-stimulated RAW264.7 cells

Duchesnea indica (Andr.) Focke (DIF), a medicinal plant from the Rosaceae family, possesses therapeutic properties such as heat-clearing and detoxification, dispersion of stasis detumescence, blood-cooling, and hemostatic effect. The latest research unveiled a diverse array of pharmacologically active compounds from DIF, showcasing a broad spectrum of pharmacological effects. However, there was limited attention given to the comprehensive investigation of its high molecular weight compounds, particularly polysaccharides. In this study, DIF extracts were prepared by water-extraction and alcohol-precipitation method and the pharmacological effect was detected. The results demonstrated that the total polysaccharide content, reducing sugar content, total flavonoid content, and total polyphenol content in DIF extracts were 32.62 ± 0.91 %, 13.41 ± 0.18 %, 1.07 ± 0.07 %, and 12.16 ± 0.27 %, respectively. Although the total antioxidant activity of DIF extracts were significantly low than that of the vitamin C group, its ability to scavenge ABTS, DPPH, and superoxide anion radicals was similar to that of the vitamin C group. Furthermore, DIF extracts demonstrated significant inhibition on NO and MDA levels while simultaneously enhancing SOD activity in LPS-stimulated RAW264.7 cells. Finally, DIF extracts significantly reduced the mRNA level of pro-inflammation cytokines interleukin (IL)-6, IL-1β, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and tumor necrosis factor (TNF)-α, and directly inhibited the phosphorylation level of extracellular regulated kinase and mitogen-activated protein kinase (ERK-MAPK) pathway. Taken together, these results indicated that DIF extracts exhibited an anti-inflammatory and antioxidant effect in LPS-induced RAW264.7 mouse macrophages by directly inhibiting ERK-MAPK signaling pathway. Based on these findings, DIF extracts provided new insights into the treatment and prevention of diseases related to oxidative stress and inflammation.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.