Biochemistry and Biophysics Reports最新文献

{"title":"Ononin combined with Lactobacillus paracasei alleviates DSS-induced ulcerative colitis by ferroptosis via inhibiting JAK2/STAT3 signaling pathway","authors":"Mei Huang ,&nbsp;Feng Yang ,&nbsp;Ju-cai Song ,&nbsp;Xue Wang ,&nbsp;Pan-pan Jiao ,&nbsp;Meng-jie Kang ,&nbsp;Qian-qian Guo ,&nbsp;Liang-zhu Si ,&nbsp;Shu-han Zhang ,&nbsp;Lin-shan Luo ,&nbsp;Yong-wei Li ,&nbsp;Wei Zhang ,&nbsp;Yue-sheng Gong ,&nbsp;Lei Dong","doi":"10.1016/j.bbrep.2026.102482","DOIUrl":"10.1016/j.bbrep.2026.102482","url":null,"abstract":"<div><h3>Background</h3><div>Ulcerative colitis (UC) is a chronic inflammatory bowel disease with a complex pathogenesis and limited treatment options. This study aimed to evaluate the therapeutic effect of Ononin combined with <em>Lactobacillus paracasei</em> in a dextran sulfate sodium (DSS)-induced murine UC model and to elucidate the underlying mechanisms involving ferroptosis and the JAK2/STAT3 signaling pathway.</div></div><div><h3>Methods</h3><div>A UC model was established by administering 3% DSS in drinking water to male BALB/c mice. Therapeutic efficacy was assessed based on changes in body weight, disease activity index (DAI), colon length, and histopathological alterations. Serum levels of inflammatory cytokines (TNF-α, IL-1β) and oxidative stress markers (MPO, MDA, SOD, GSH) were quantified. Protein expression related to ferroptosis (ACSL4, GPX4), intestinal barrier integrity (Occludin, Claudin-1), and the JAK2/STAT3 pathway was analyzed in colon tissue.</div></div><div><h3>Results</h3><div>Combined treatment with Ononin and <em>Lactobacillus paracasei</em> significantly alleviated UC symptoms, as shown by improved body weight, colon length, DAI scores, and histopathology. It also reduced pro-inflammatory cytokine levels, suppressed oxidative stress and neutrophil infiltration, and enhanced antioxidant capacity. Mechanistically, the treatment downregulated ACSL4 expression, inhibited JAK2/STAT3 phosphorylation, and upregulated the expression of GPX4, Occludin, and Claudin-1.</div></div><div><h3>Conclusion</h3><div>The combination of Ononin and <em>Lactobacillus paracasei</em> effectively ameliorated DSS-induced colitis in mice. Its protective mechanism involves suppressing inflammation and oxidative stress, inhibiting ferroptosis (potentially through blocking the JAK2/STAT3 pathway), and enhancing intestinal barrier integrity. These findings provide a strong preclinical rationale for developing this combination as a potential therapeutic strategy for UC.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"45 ","pages":"Article 102482"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the bioactive potential of bovine pericardium membrane combined with hyaluronic Acid: characterization and cellular viability analyses","authors":"Dwi Wahyu Indrawati ,&nbsp;Ernie Maduratna Setiawatie ,&nbsp;Retno Pudji Rahayu ,&nbsp;Rizky Briliant Syah Manurung ,&nbsp;Mohammed Ahmed Aljunaid","doi":"10.1016/j.bbrep.2026.102490","DOIUrl":"10.1016/j.bbrep.2026.102490","url":null,"abstract":"<div><div>This study explores the bioactive potential of bovine pericardium membranes combined with hyaluronic acid (HA, 120 kDa) at concentrations of .5%, 1.0%, and 2.0% through physicochemical characterization and fibroblast cell viability analysis. A laboratory experimental design was employed to evaluate bovine pericardium membranes modified with hyaluronic acid at different concentrations. Material characterization was conducted employing Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and X-ray Diffraction (XRD) analyses. Fibroblast cell viability was assessed using the MTT assay, and statistical analysis was conducted using the ANOVA test. The results demonstrated that hyaluronic acid modification altered the physicochemical characteristics of the membrane, including increased surface hydrophilicity. The cell viability test revealed comparable fibroblast viability among HA-modified membranes, indicating no cytotoxic effects. FTIR and SEM analyses confirmed chemical interactions and morphological features associated with favorable cell–material interactions. These findings suggest that bovine pericardium membrane with hyaluronic acid 120 kDa exhibit favorable physicochemical characteristics and in vitro cytocompatibility, indicating their potential for tissue engineering applications. This research provides a scientific foundation for developing innovative biomaterials that support favorable cell–material interactions for tissue regeneration research, contributing to advancements in tissue regeneration therapy.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"45 ","pages":"Article 102490"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential role of circ_WHSC1 and miR-145-5p in breast cancer promotion","authors":"Maryam Abtin ,&nbsp;Asghar Hosseinzadeh ,&nbsp;Nahid Nafisi ,&nbsp;Ramesh Omranipour ,&nbsp;Leyla Sahebi ,&nbsp;Mohsen Ahmadi ,&nbsp;Soudeh Ghafouri-Fard ,&nbsp;Abbas Shakoori","doi":"10.1016/j.bbrep.2026.102472","DOIUrl":"10.1016/j.bbrep.2026.102472","url":null,"abstract":"<div><h3>Purpose</h3><div>This study aims to investigate the potential interplay between <em>circ_WHSC1</em> and <em>miR-145-5p</em> in breast cancer pathogenesis using <em>in silico</em> tools, assess their clinical relevance, and evaluate the diagnostic utility of <em>circ_WHSC1</em> in clinical samples as a biomarker for breast cancer.</div></div><div><h3>Materials and methods</h3><div>This multi-component study employed a combination of bioinformatic analyses and laboratory validation. First, <em>in silico</em> tools were used to investigate <em>miR-145-5p</em> and <em>circ_WHSC1</em> using public databases. Subsequently, their expression, correlation, and clinical relevance were experimentally assessed in a cohort of breast cancer patients.</div></div><div><h3>Results</h3><div><em>Circ_WHSC1</em> level was significantly higher in breast tumor compared with patient-matched adjacent normal tissues (4.17-fold change, p-value &lt;0.01). Additionally, significant reduction of <em>miR-145-5p</em> (9.11-fold downregulation, p-value &lt;0.01) level was detected in breast tumors compared with neighboring non-tumor tissues. A weak negative correlation was detected between levels of <em>circ_WHSC1</em> and <em>miR-145-5p</em> (r = −0.314, p-value &lt;0.05). <em>Circ_WHSC1</em> may serve as a weak biomarker for breast cancer (AUC = 0.683; p-value &lt;0.01) with 71 % specificity and 70 % sensitivity. Up-regulation of <em>circ_WHSC1</em> in breast tumor was linked with lymph node invasion (p-value = 0.005), HER2 negativity (p-value = 0.031) and positive family history (p-value = 0.012).</div></div><div><h3>Conclusion</h3><div>Cumulatively, <em>circ_WHSC1/miR-145-5p</em> can be suggested as a potential molecular axis contributing to the pathogenesis of breast cancer. However, further functional assays are needed to validate this hypothesis.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"45 ","pages":"Article 102472"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive multi-omics analysis reveals RUNX1's prognostic value, immune associations, and MUC13-Mediated mechanistic role in hepatocellular carcinoma pathogenesis","authors":"Tahani Ahmad ALMatrafi","doi":"10.1016/j.bbrep.2026.102446","DOIUrl":"10.1016/j.bbrep.2026.102446","url":null,"abstract":"<div><div>Hepatocellular carcinoma (HCC) is a biologically and clinically heterogeneous malignancy, posing major challenges for early diagnosis, prognostic prediction, and therapeutic intervention. The Runt-related transcription factor (RUNX) family, comprising RUNX1, RUNX2, and RUNX3, plays essential roles in cellular processes and contribute to the pathogenesis of numerous malignancies. This study aims to elucidate their role in tumor progression and its clinical implications. We performed an integrative pan-cancer multi-omics analysis across 33 cancer types to characterize RUNX family expression patterns, prognostic associations, and immune correlations using publicly available datasets. Expression and functional roles were experimentally validated in HCC through <em>in vitro</em> and <em>in vivo</em> assays. Relationships between <em>RUNX1</em> expression and clinical stage, immune subtypes, checkpoint gene expression, immunotherapy sensitivity, and immune-related co-expression networks were assessed. The prognostic significance of <em>RUNX1</em> was determined using Kaplan–Meier survival analysis, Cox regression, and ROC curve evaluation. Transcriptional regulation was investigated via bioinformatic tools, database interrogation, and chromatin immunoprecipitation (ChIP) assays. Functional studies were conducted to determine the effects of <em>RUNX1</em> knockdown on HCC cell proliferation, migration, and invasion. <em>RUNX1</em> was upregulated in multiple tumor types, including breast, lung, liver, and brain cancers, and its elevated expression correlated with poorer overall (OS) and disease-specific survival (DSS). In HCC, RUNX1 expression was significantly associated with advanced clinical stage, distinct immune subtypes, and altered checkpoint expression profiles, indicating a role in shaping the tumor immune microenvironment. Mechanistically, <em>MUC13</em> was identified as a direct transcriptional target of <em>RUNX1</em>, as confirmed by promoter-binding prediction, ChIP validation, and dual-luciferase assays. <em>RUNX1</em> knockdown suppressed HCC cell proliferation, migration, and invasion by attenuating Wnt/β-catenin/EMT signaling activity. These findings highlight RUNX1 as a potential prognostic biomarker and therapeutic target, offering mechanistic insight into HCC pathogenesis and avenues for improved precision oncology.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"45 ","pages":"Article 102446"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146163867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA binding protein HuD regulates the biosynthesis of glucagon-like peptide 1 in intestinal L-cells","authors":"Sukyoung Han ,&nbsp;Myeongwoo Jung ,&nbsp;Seungyeon Ryu ,&nbsp;Seongho Cha ,&nbsp;Hyosun Tak ,&nbsp;Seung Min Jeong ,&nbsp;Eun Kyung Lee","doi":"10.1016/j.bbrep.2025.102415","DOIUrl":"10.1016/j.bbrep.2025.102415","url":null,"abstract":"<div><div>We investigated HuD (ELAVL4), a neuronal RNA-binding protein with emerging endocrine functions, as a regulator of GLP-1 biogenesis in intestinal L-cells. Using absolute qPCR and immunofluorescence, we found that HuD was significantly expressed in the GLUTag cells derived from mice intestinal L-cell line and was co-localized with GLP-1-positive cells in the small intestine of mice. RNA interference-mediated HuD knockdown reduced both GLP-1 immunoreactivity and levels. Mechanistically, RNA immunoprecipitation and biotinylated 3′UTR pull-down demonstrated that HuD binds to the mRNAs of the <em>Gcg</em> and <em>Pcsk1</em> (PC1/3) genes. The levels of the proglucagon (∼17 kDa) and PC1/3 proteins decreased without significant changes in their mRNAs, suggesting post-transcriptional control. Metabolic stress converged on this module: palmitate decreased proglucagon/PC1/3 in GLUTag cells, and a high-fat diet reduced GLP-1 and PC1/3 signals in the mouse intestine. Interestingly, zinc sulfate partially restored proglucagon and PC1/3 levels following palmitate treatment. These findings reveal a HuD–<em>Gcg</em>/<em>Pcsk1</em> axis is crucial for GLP-1 biogenesis and which is susceptible to lipotoxic stress.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"45 ","pages":"Article 102415"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145797523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and analysis of small RNAs in exosome-like nanoparticles derived from onion (Allium cepa L.)","authors":"Zhaofeng Liang ,&nbsp;Yuning Gu ,&nbsp;Jiajia Song ,&nbsp;Wenhui Yang ,&nbsp;Xuezhong Xu","doi":"10.1016/j.bbrep.2026.102460","DOIUrl":"10.1016/j.bbrep.2026.102460","url":null,"abstract":"<div><div>Small RNAs (sRNAs) in plant-derived exosome-like nanoparticles (PELNs) have attracted considerable attention as biologically active substances that play a crucial role in cross-kingdom regulatory processes. However, there is insufficient research dedicated to identifying sRNAs within PELNs. We aimed to analyze the expression profile of sRNAs in onion-derived exosomes-like nanoparticles (OELNs) and explore their potential role in cross-kingdom regulation. In this study, OELNs were extracted, possessing an optimal particle size of approximately 139 nm and a Zeta potential of roughly −20 mV. Subsequently, high-throughput small RNA sequencing was conducted to analyze sRNAs in onion tissues and OELNs. The expression profile of sRNAs in OELNs was elucidated, and it was predicted that highly expressed microRNAs (miRNAs) in OELNs may exert potential regulatory effects on the human genome. By comparing with the reference genome of onion (<em>Allium cepa L.</em>), 11 known miRNAs and 248 novel miRNAs were identified in both onion tissues and OELNs. Target gene prediction analysis of the human genome has revealed that 20 miRNAs highly expressed in OELNs potentially participate in the regulation of human tumor and cardiovascular-related signaling pathways. Furthermore, differential analysis was conducted on small/short interfering RNA (siRNA), small nucleolar RNA (snoRNA), and transfer ribonucleic acid (tRNA) in OELNs. This study laid a theoretical foundation for further exploring the cross-kingdom role of non-coding RNAs in OELNs, and provided new ideas for the development of plant-derived functional components.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"45 ","pages":"Article 102460"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breast cancer susceptibility gene 2 upregulation alleviated cardiac hypertrophy in angiotensin II-treated mice","authors":"Kun Liu ,&nbsp;Xiao-Xuan Gong ,&nbsp;Yong Li ,&nbsp;Ming-Zhu Li ,&nbsp;Chen Si ,&nbsp;Lei Zhou","doi":"10.1016/j.bbrep.2026.102445","DOIUrl":"10.1016/j.bbrep.2026.102445","url":null,"abstract":"<div><div>Loss of breast cancer susceptibility gene 2 (BRCA2) function was found to exacerbate doxorubicin-mediated cardiomyocyte apoptosis and promote heart failure progression. We hypothesized that upregulation of BRCA2 may alleviate hypertrophic cardiomyopathy. Hypertrophic cardiomyopathy was established in mice via chronic angiotensin II (Ang II) administration (1.44 mg/kg/day) using osmotic minipumps. Cardiac BRCA2 expression was significantly downregulated in Ang II-treated mice. Cardiac hypertrophy triggered by Ang II in mice was significantly attenuated upon BRCA2 overexpression. Similarly, in cultured primary cardiomyocytes, Ang II-induced hypertrophic responses were suppressed by BRCA2 upregulation. The cardiac fibrosis was significantly attenuated after upregulation of BRCA2 in Ang II-induced hypertrophic cardiomyopathy. The myocardial inflammatory response to Ang II, characterized by elevated interleukin (IL)-1β, IL-6, and tumor necrosis factor alpha (TNF-α) levels, was markedly reduced with BRCA2 overexpression. The apoptotic biomarkers including Bax and cleaved caspase 3 (CC3) increased in the heart of hypertrophic cardiomyopathy, and attenuated after upregulation of BRCA2. These results indicated that upregulation of BRCA2 could improve hypertrophic cardiomyopathy. BRCA2 alleviated cardiac hypertrophy via attenuation of inflammation and apoptosis.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"45 ","pages":"Article 102445"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the molecular landscape of clear cell renal cell carcinoma through integrative transcriptomic analysis and validation using clinical samples","authors":"Amir Taherkhani ,&nbsp;Simindokht Sarvmeili ,&nbsp;Hamed Manoochehri ,&nbsp;Mahmoud Gholyaf","doi":"10.1016/j.bbrep.2025.102384","DOIUrl":"10.1016/j.bbrep.2025.102384","url":null,"abstract":"<div><h3>Background and aims</h3><div>Clear cell renal cell carcinoma (ccRCC) is the most prevalent subtype of renal malignancies, distinguished by its aggressive nature and poor prognosis in advanced stages. Identifying prognostic biomarkers and unraveling underlying molecular events contributing to ccRCC pathogenesis is crucial for developing precise prognostic models and tailored therapeutic interventions. The objective of this research was to identify differentially expressed genes (DEGs), construct protein-protein interaction networks, pinpoint hub genes and enriched pathways, assess the prognostic relevance of these hub genes, and predict upstream regulators, thereby providing insights into the onset and progression of ccRCC.</div></div><div><h3>Methods</h3><div>The GSE66270 dataset was reanalyzed to uncover DEGs between 14 ccRCC tumors and 14 normal tissues. Hub genes, clusters, and enriched functional categories were identified from the protein-protein interaction network. Survival analysis was performed to assess the prognostic relevance of the identified hub genes. An upstream transcription factor network was generated using the iRegulon plugin. Expression of hub genes in six cancer and six normal renal tissues was confirmed via real-time PCR.</div></div><div><h3>Results</h3><div>A prognostic signature comprising five genes—<em>FCGR1A</em>, <em>FOXM1</em>, <em>TOP2A</em>, <em>BIRC5</em>, and <em>CCNA2</em>—effectively stratified ccRCC prognosis. <em>FOXM1</em> was identified as the primary upstream regulator. A significant upregulation of <em>FOXM1</em> was observed in renal cancer samples compared with normal renal tissues (p-value &lt;0.001).</div></div><div><h3>Conclusion</h3><div>These findings shed light on implicated pathways and processes, prognostic biomarkers, and the crucial role of the immune system in ccRCC pathogenesis.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"45 ","pages":"Article 102384"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of activation of macrophages/microglia in diabetic retinopathy and Familial Exudative Vitreoretinopathy","authors":"Jiakai Li ,&nbsp;Ping Fei ,&nbsp;Xiang Zhang ,&nbsp;Yuqing Rao ,&nbsp;Jing Li ,&nbsp;Peiquan Zhao","doi":"10.1016/j.bbrep.2025.102396","DOIUrl":"10.1016/j.bbrep.2025.102396","url":null,"abstract":"<div><div>Familial Exudative Vitreoretinopathy (FEVR) and Diabetic Retinopathy (DR) are two prominent retinal diseases. The role of macrophages/microglia in the vascular dynamics of FEVR and DR is unknown and thus addressed in this study. FZD4 knockout mouse, a model for FEVR in human characterized by genetic mutations affecting angiogenesis, exhibited reduced b-wave amplitudes and decreased vascular density, replicating human FEVR symptoms. Conversely, STZ-treated C57/BL6 mouse developed heightened fasting glucose levels, reduced insulin content, and increased retinal vasculature, aligning with DR features. Further analysis revealed significant differences in macrophage/microglia populations between the two diseases. In DR, a marked increase in both number and M2-like polarization of retinal macrophages/microglia was observed, contrasting with FEVR. Moreover, DR induced substantial proinflammatory differentiation of macrophages/microglia, evidenced by elevated cytokines such as IL-1β, TNF-α, and IFNɣ. Both conditions significantly upregulated Ang-1 and IL-10, with a more pronounced IL-10 increase in DR, suggesting a more active role in tissue and vessel remodeling. Notably, DR induced higher levels of anti-inflammatory factors like bFGF, TIMP-1, TGFβ1, and VEGF-A compared to FEVR, suggesting a balance of inflammation initiation, progression and resolution. These findings highlight the distinct roles of macrophages/microglia in FEVR and DR, providing insights into their contributions to disease pathogenesis and potential therapeutic strategies through reprogramming macrophages/microglia.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"45 ","pages":"Article 102396"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of PLIN5 and lipid composition on lipid droplet contact sites with other organelles","authors":"Mahsa Mohammadian ,&nbsp;Shima Asfia ,&nbsp;Ralf Seemann","doi":"10.1016/j.bbrep.2025.102402","DOIUrl":"10.1016/j.bbrep.2025.102402","url":null,"abstract":"<div><div>Lipid droplets (LDs) maintain cellular lipid homeostasis through dynamic interactions with other organelles. Understanding how these contact sites form is crucial for uncovering the mechanisms of lipid exchange and signaling. In this study, we used an in vitro model to investigate how lipid composition and the LD-associated protein perilipin 5 (PLIN5) influence contact formation between an LD monolayer and a bilayer membrane. Artificial LDs consisting of triolein and coated with either a DOPE or DOPC monolayer containing PLIN5 or not were incubated with large unilamellar vesicles (LUVs) that mimic the bilayer membrane of the organelle. Using double fluorescence labeling of the LUV bilayer and the core, we can distinguish between fusion of the LUV bilayer with the LDs and stable attachment of LUVs to the LD’s surface. Our results show that the probability of fusion between LDs and LUVs is greatly increased for DOPE-coated LDs, while PLIN5 promotes the stable attachment of LUVs to the LD’s surface and prevents fusion. These observations illustrate how certain lipid and protein components can modulate contact formation between LDs and membranes in a controlled in vitro system, and provide a basis for future studies on the molecular mechanisms of organelle communication.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"45 ","pages":"Article 102402"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145748904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}