Phyllis C Pugh, Bijay R Khanal, Jenna L Lemons, Mario A Murillo, Jacika N Patel, Preeya K Boppana, Veera Padmanabhan
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引用次数: 0
Abstract
Complex, chemically-undefined media components are often used as nutrients in the production of biological products via mammalian cell culture. Variability in the compositions of these complex raw materials can significantly impact product yields. This paper investigates the influence of raw material quality on the cell culture process by developing data-based models to estimate final productivity in an industrial antibody production operation at AstraZeneca. Fourier Transform Infrared (FTIR) spectroscopy measurements of selected raw material components were obtained. These measurements were processed, derivatized, and used to create Partial Least Squares (PLS) regression chemometric models. The resulting models were then employed to predict the influence of such raw variability on the yields of biotherapeutic molecules.
期刊介绍：
Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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