Biochemistry and Biophysics Reports最新文献

{"title":"Ozonated olive oil inhibits melanoma proliferation by inducing ferroptosis","authors":"Seong-Jin An ,&nbsp;Jun-Ichi Kashiwakura ,&nbsp;Akira Katsuyama ,&nbsp;Sumihito Togi ,&nbsp;Yuichi Kitai ,&nbsp;Ryuta Muromoto ,&nbsp;Toshiaki Miura ,&nbsp;Satoshi Ichikawa ,&nbsp;Tadashi Matsuda","doi":"10.1016/j.bbrep.2025.102267","DOIUrl":"10.1016/j.bbrep.2025.102267","url":null,"abstract":"<div><div>Although ozone is a potent oxidant that can damage lungs and skin after prolonged exposure, ozonated olive oil (OZO) exhibits antimicrobial, anti-inflammatory, and wound-healing effects. Here, we describe a novel application of OZO in melanoma therapy. Treatment with OZO markedly inhibited the proliferation of both human and murine melanoma cells, while sparing normal human keratinocyte. At the molecular level, OZO upregulated ferroptosis-related genes, decreased intracellular glutathione (GSH) and GPX4 protein levels and accelerated lipid peroxidation. Critically, OZO-induced growth inhibition in melanoma cells was prevented by ferroptosis inhibitors (ferrostatin-1 and deferiprone), but not by inhibitors of apoptosis or necroptosis. Taken together, these findings offer new therapeutics strategy for treating melanoma by inducing ferroptosis.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102267"},"PeriodicalIF":2.2,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of Senecavirus A using a colloidal gold lateral flow strip","authors":"Xiaomeng Pei ,&nbsp;Jing Wang ,&nbsp;Zhijing Wei ,&nbsp;Yuanying Li ,&nbsp;Meng Wu","doi":"10.1016/j.bbrep.2025.102240","DOIUrl":"10.1016/j.bbrep.2025.102240","url":null,"abstract":"<div><div>Senecavirus A (SVA) could cause vesicular lesions and other symptoms in pigs, which has resulted in substantial economic losses to the swine industry. To establish a rapid detection method for SVA, an immunochromatographic lateral flow strip was constructed. First, the recombinant SVA VP2 protein was purified, and the monoclonal antibodies were prepared. 4B10 was coated onto the test line (T line), and 1E3 was colloidal gold-labeled to assemble the colloidal gold test strip. The constructed LFA strip showed a detection limit of 0.5 ng/mL, and no cross-reactivity with FMDV, PRRSV, CSFV and PCV2. Clinical samples validation showed 90 % concordance with RT-PCR results, indicated the reliability of this method. The developed lateral flow assay (LFA) provided a rapid and simple method for point-of-care testing of SVA.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102240"},"PeriodicalIF":2.2,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and external validation of a prognostic signature based on myeloid-derived suppressor cells-related LncRNAs to evaluate survival prognosis and treatment efficacy in invasive breast carcinoma","authors":"Yun Zhong ,&nbsp;Tong Mu ,&nbsp;Shunyao Zhang ,&nbsp;Yuan Xiang ,&nbsp;Lei Xie ,&nbsp;Wenxiong Zhang ,&nbsp;Kang Wang","doi":"10.1016/j.bbrep.2025.102261","DOIUrl":"10.1016/j.bbrep.2025.102261","url":null,"abstract":"<div><h3>Background</h3><div>Originating in the hematopoietic tissue, myeloid-derived suppressor cells (MDSCs) significantly contribute to tumor-related immunological processes. However, their relationship with long noncoding RNAs (lncRNAs) and breast cancer remains incompletely understood. In this study, we introduced MDSCs-associated lncRNAs as novel prognostic biomarkers to assess outcomes in patients with invasive breast carcinoma (BRCA).</div></div><div><h3>Methods</h3><div>Information regarding BRCA cases, including clinical and genomic details, was obtained from the TCGA repository. Predictive indicators were discovered, and their reliability underwent thorough verification. A clinically useful nomogram was developed following application-based validation. Additional investigations encompassed functional analysis, TMB assessment, TME profiling, immunotherapy efficacy forecasting, and drug sensitivity testing along with target identification. Long non-coding RNA expression was measured using reverse transcription quantitative PCR.</div></div><div><h3>Results</h3><div>A risk stratification model incorporating eight MDSCs-related lncRNAs effectively predicted patient outcomes. Kaplan-Meier (K-M) survival analysis clearly indicated a much worse prognosis among patients classified as high-risk (p &lt; 0.001). The nomogram accurately forecasted overall survival (OS). Analysis of functional enrichment revealed that pathways associated with epithelial cells showed activity among patients at higher risk. Characterization of the tumor microenvironment showed increased immune cell presence in those classified as low-risk. Conversely, individuals with greater risk displayed higher tumor mutational burden. TIDE and IPS analyses indicated superior immunotherapy responsiveness in the low-risk BRCA subgroup. Among 47 drugs with notable IC50 variations, Ribociclib, PD173074, KU-55933, NU7441, and nutlin-3a exhibited lower IC50 values within the low-risk group, whereas Lapatinib demonstrated greater efficacy among the high-risk group. Moreover, 10 potential therapeutic agents and their targets were predicted for high-risk patients. RT-qPCR validation confirmed the robustness of the model.</div></div><div><h3>Conclusions</h3><div>We successfully verified a new model of molecular markers of MDSCs-related lncRNAs, offering critical insights for predicting outcomes and guiding therapeutic decisions in BRCA cases.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102261"},"PeriodicalIF":2.2,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LIN28-mediated gene regulatory loops synchronize transitions throughout organogenesis","authors":"Indhujah Thevarajan ,&nbsp;Maria F. Osuna ,&nbsp;Sonia Fuentes Lewey ,&nbsp;Eustolia Sauceda ,&nbsp;Sayra Briseno ,&nbsp;Caylah Griffin ,&nbsp;Bareun Kim ,&nbsp;R. Grant Rowe ,&nbsp;Edroaldo Lummertz da Rocha ,&nbsp;Jihan K. Osborne","doi":"10.1016/j.bbrep.2025.102226","DOIUrl":"10.1016/j.bbrep.2025.102226","url":null,"abstract":"<div><div>Control of the intervals between proliferation and differentiation of stem/progenitor cells is coordinated by developmental regulators, comprised of both microRNAs (miRNAs) and proteins, termed heterochronic genes. The heterochronic factors, Lin28-RNA-binding proteins (RBPs) and the miRNAs–<em>Let-7,</em> comprise a unique subset of evolutionarily conserved genes that regulate the developmental timing of metazoans, from worms to mammals. While there has been much investigation into the reciprocal negative feedback loop between LIN-28 and <em>Let-7</em> during fetal development and cancer. Few have investigated how positive regulatory loops between the mammalian Lin28-RBPs, and mRNAs order spatiotemporal transitions of progenitors from specification to organogenesis. Screening for factors that activated luciferase reporters of the human <em>LIN28A</em> and <em>LIN28B</em> promoters, in combination with genetic mouse models, we demonstrate positive feedforward loops between key developmental transcription factors such as B-Catenin, Sox2, Sox9, and Lin28-RBPs. Furthermore, we demonstrate heterochronic regulation of morphogenesis is not only genetically modulated but also molecularly fine-tuned via position-dependent sequences in the 5′ and/or 3’ untranslated regions.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102226"},"PeriodicalIF":2.2,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the effect of HMGB1 on immune cells isolated from SLE mice","authors":"Xin Qin,&nbsp;Jinglin Gao,&nbsp;Xiuhua Liu,&nbsp;Jie Chen,&nbsp;Xinghui Song","doi":"10.1016/j.bbrep.2025.102262","DOIUrl":"10.1016/j.bbrep.2025.102262","url":null,"abstract":"<div><div>Systemic lupus erythematosus (SLE) is a complex autoimmune disease, and its pathogenesis is related to the balance and dysfunction of dendritic cells (DCs). The therapy targeting dendritic cells has become an important means to treat SLE and autoimmune diseases. Besides, HMGB1 is highly expressed in the serum of patients with SLE. Therefore, this study intended to find the function of HMGB1 on immune cells in SLE. We used the MRL/lpr mice as SLE model animals, then we isolated the peripheral blood of SLE mice to obtain CD14⁺ cells, which was induced into monocyte-derived Dendritic cells (Mo-DCs). We also obtained CD14⁺ cells from peripheral blood to explore the effect of HMGB1 treated Mo-DCs. Then followed the HMGB1 treatment, flow cytometry, ELISA, WB and CCK8 assay. The results showed that HMGB1 can stimulate the maturation of DCs, and activated mTOR pathway, and affect the proliferation and differentiation of CD4⁺ cells. What's more, HMGB1 seemed to enter cells through the endocytosis of Toll-like receptor, and its function maybe related to endoplasmic reticulum stress. The further research found tunicamycin (endoplasmic reticulum stress inducer) and rapamycin (mTOR inhibitor) can inhibit the activation of Mo-DCs. Therefore, we thought that HMGB1 enters DCs by TLR6 and regulate the activation of DCs and the differentiation of CD4⁺ cells by influencing endoplasmic reticulum stress and the mTOR pathway.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102262"},"PeriodicalIF":2.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metformin interferes with urinary creatinine measurement using enzymatic method","authors":"Akira Yoshimoto ,&nbsp;Yoshifumi Morita ,&nbsp;Yukio Kume ,&nbsp;Naoyuki Yoshikawa ,&nbsp;Yoshikazu Ono ,&nbsp;Makoto Kurano ,&nbsp;Yutaka Yatomi ,&nbsp;Ryunosuke Ohkawa","doi":"10.1016/j.bbrep.2025.102264","DOIUrl":"10.1016/j.bbrep.2025.102264","url":null,"abstract":"<div><div>Abnormal reaction curves were observed in urinary creatinine tests in patients with diabetes undergoing metformin therapy. Therefore, we investigated whether metformin interferes with urinary creatinine measurements.</div><div>First, the reaction curves of urinary creatinine measurements from 328 patients were analyzed, focusing on the maximum reaction speed, final reaction speed, and their ratio. Next, the reaction curves of the creatinine solution with and without metformin were analyzed. To elucidate the mechanism of metformin interference, solutions of creatinine, creatine, sarcosine, and hydrogen peroxide with and without metformin were analyzed using a one-reagent measurement in which reagents 1 and 2 were pre-mixed.</div><div>As the results, in the 84 patients taking metformin, the maximum reaction speed significantly decreased, whereas the final reaction speed and the ratio of the two speeds significantly increased (p &lt; 0.001). Notably, the area under the curve of the ratio of the two speeds was 0.84 (95 % confidence interval: 0.78–0.89) for detecting metformin use, suggesting that metformin inhibits the series of reactions involved in creatinine measurement and that reaction curve analysis can identify metformin use. Similar results were obtained in experiments using metformin-containing creatinine solutions. Metformin did not interfere with the reactions involving sarcosine or hydrogen peroxide but did interfere with those involving creatinine and creatine, indicating that metformin inhibits creatinase activity.</div><div>In conclusion, metformin inhibits reactions involved in urinary creatinine measurement in urine samples, potentially leading to falsely low urinary creatinine values and an inaccurate assessment of kidney function in patients with diabetes.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102264"},"PeriodicalIF":2.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epitope analysis of an anti-mouse CCR1 monoclonal antibody S15040E using flow cytometry","authors":"Ayaka Okada ,&nbsp;Hiroyuki Suzuki ,&nbsp;Takao Arimori ,&nbsp;Tomohiro Tanaka ,&nbsp;Mika K. Kaneko ,&nbsp;Yukinari Kato","doi":"10.1016/j.bbrep.2025.102265","DOIUrl":"10.1016/j.bbrep.2025.102265","url":null,"abstract":"<div><div>The C–C motif chemokine receptor 1 (CCR1) is widely expressed in various immune cells and plays crucial roles in the maturation and migration of immune cells. CCR1 has been considered an attractive drug target for treating autoimmune diseases and tumors. An anti-mouse CCR1 (mCCR1) monoclonal antibody (clone S15040E) has been used in various <em>in vivo</em> studies to identify mCCR1-positive cells by flow cytometry. However, the binding epitope has not been determined. This study investigated the binding epitope of S15040E using flow cytometry. The mCCR1 extracellular domain-substituted mutant analysis showed that S15040E recognizes the extracellular loop 2 (ECL2, aa 172–197) of mCCR1. Next, alanine (or glycine) scanning was conducted in the ECL2 region. The results revealed that Trp176, Phe178, and Arg181 are essential amino acids for the recognition by S15040E. These results showed the involvement of the ECL2 of mCCR1 in the recognition by S15040E.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102265"},"PeriodicalIF":2.2,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chinese traditional medicine complex hemorrhoidal analgesic cream promotes wound healing in diabetic rats after anal fistula surgery via Keap1/Nrf2 signaling pathway","authors":"Jian Liu ,&nbsp;Zhitao Yin ,&nbsp;Xiaojun Tian ,&nbsp;Delong Liu ,&nbsp;Chunxiao Wang ,&nbsp;Hexue Yuan","doi":"10.1016/j.bbrep.2025.102258","DOIUrl":"10.1016/j.bbrep.2025.102258","url":null,"abstract":"<div><div>This study to elucidate how hemorrhoidal analgesic cream (HAC), grounded in the traditional Chinese medicine (TCM) ‘elevation and causation’ theory, promotes postoperative wound healing after anal fistula surgery in diabetic rats by modulating the Keap1/Nrf2 signaling pathway. We used high-sugar and high-fat chow combined with streptozotocin (STZ) to induce diabetic rats to establish a postoperative wound model of anal fistula. The rats were divided into a blank control group, model group, wound-only group, experimental group (HAC treatment), and positive control group (recombinant human epidermal growth factor gel). HAC's impact on wound healing was evaluated by glycated hemoglobin (HbA1c), serum inflammatory factors (TNF-α, IL-6), oxidative stress markers (SOD, MDA), and Nrf2 pathway proteins and mRNA. The results showed that the wound healing rate was significantly increased in the HAC group (92.85 ± 3.41 %, <em>P</em> &lt; 0.01), the levels of HbA1c were decreased (<em>P</em> &lt; 0.05), the levels of TNF-α, IL-6, and MDA were decreased (<em>P</em> &lt; 0.01), the activity of SOD was increased (<em>P</em> &lt; 0.05), and the Keap1/Nrf2 signaling pathway was activated. The study demonstrated that HAC could reduce oxidative stress and inflammatory responses by regulating the Keap1/Nrf2 signaling pathway, which validates its scientific value in promoting wound healing after diabetes mellitus (DM) fistula surgery.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102258"},"PeriodicalIF":2.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oenological tannins mitigate rotenone-induced mitochondrial impairments and oxidative stress, with concomitant detection of urolithin A in the brain","authors":"Olga Wojciechowska ,&nbsp;Michaël Jourdes ,&nbsp;Mirosław Andrusiewicz ,&nbsp;Małgorzata Pokrzywa ,&nbsp;Marta Karaźniewicz-Łada ,&nbsp;Jadwiga Jodynis-Liebert ,&nbsp;Pierre-Louis Teissedre ,&nbsp;Małgorzata Kujawska","doi":"10.1016/j.bbrep.2025.102263","DOIUrl":"10.1016/j.bbrep.2025.102263","url":null,"abstract":"<div><div>Mitochondria are key organelles that supply energy to the brain, and their dysfunction contributes to neurotoxicity induced by environmental toxins such as rotenone. Recently, oenological tannins (OTs) and their colonic metabolite, urolithin A (UA), have been emphasized due to their potential neuroprotective activity. However, their role in counteracting toxin-induced mitochondrial impairments remains unclear. Therefore, this study aimed to investigate the administration of OTs to rotenone (ROT)-induced mitochondrial dysfunction and oxidative stress, key contributors to neurotoxicity. We measured mitochondrial membrane potential (MMP), the activity of mitochondrial complex I (Grishchuk et al.), and aldehyde dehydrogenase 2 (ALDH2) to assess mitochondria and protein carbonyl (PC) levels. We also checked the presence of UA in the brain. Our results indicate that the OTs treatment restored MMP, increased MCI and ALDH2 activity, and decreased PC content in ROT-induced rats. Furthermore, we confirmed the presence of UA in the brains of the animals. While its exact contribution to the observed mitochondrial effects remains undetermined, this finding suggests a potential role of the gut-derived metabolite in neuroprotection. Thus, we conclude that OTs administration attenuates mitochondria-related neurotoxicity. We call for further mechanistic studies and the putative contribution of metabolites, including UA, to the demonstrated mitoprotective effect of OTs treatment.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102263"},"PeriodicalIF":2.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of IgG+ serum on Zika virus infection response in a model of neural precursor cells","authors":"Ismael Plentz ,&nbsp;Douglas Pazzin ,&nbsp;Thales Previato ,&nbsp;Fernanda Wagner ,&nbsp;Marina Boff ,&nbsp;Larissa Fernandes ,&nbsp;João Ismael B. Gonçalvez ,&nbsp;Daniel Marinowic ,&nbsp;Jaderson Costa da Costa","doi":"10.1016/j.bbrep.2025.102260","DOIUrl":"10.1016/j.bbrep.2025.102260","url":null,"abstract":"<div><div>The high frequency of Zika virus (ZIKV) infection prompted the World Health Organization (WHO) to declare it an emerging threat in 2016. Presently, countries in South America continue to report a significant number of Zika virus cases. Zika virus infection has been associated with neurological diseases. This article explores the impact of ZIKV infection on IgG + Zika-reactive conditions using a neurosphere model. For this investigation, neurospheres derived from Wistar rats were exposed to the Zika virus and to IgG + conditions reactive to Zika. The relative expression levels of the NOTCH-1, HES-1, and HEY-1 genes were assessed through quantitative Polymerase Chain Reaction (qPCR). Additionally, the concentrations of GCSF and IL-10 proteins were quantified using a Luminex immunoassay. Morphological evaluation of the neurospheres was conducted, with parameters such as area and irregularity rate being analyzed using Image Pro Plus 7 software.The irregularity rate of ZIKV neurospheres decreased at its lowest concentration; however, our morphology analysis was insufficient to fully elucidate the impact of ZIKV on neurospheres. Interestingly, IgG + serum exhibited neuroprotective effects against subsequent Zika virus exposure, restoring NOTCH-1 and HES-1 expression levels to normal. HEY-1 expression remained unaffected by Zika virus exposure but decreased with IgG + serum. Surprisingly, levels of the anti-inflammatory markers GCSF and IL-10 showed no significant changes. These findings highlight the complex interplay between ZIKV infection, immune response, and neurodevelopmental processes. Further research is necessary to elucidate the precise mechanisms underlying these observations and explore potential therapeutic interventions.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102260"},"PeriodicalIF":2.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}