Biochemistry and Biophysics Reports最新文献

{"title":"Effect of β-catenin on hypoxia induced epithelial mesenchymal transition in HK-2 cells by regulating Brachyury","authors":"Ping Sun ,&nbsp;Haihui Yang ,&nbsp;Binying Min ,&nbsp;Yongfu Li ,&nbsp;Jun Wang ,&nbsp;Mo Chen ,&nbsp;Diping Yu ,&nbsp;Wenjuan Sun","doi":"10.1016/j.bbrep.2024.101907","DOIUrl":"10.1016/j.bbrep.2024.101907","url":null,"abstract":"<div><h3>Background</h3><div>Chronic kidney disease (CKD) has become a worldwide health problem and the incidence rate and mortality of CKD have been rising. Renal fibrosis (RF) is the final common pathological feature of almost all kinds of CKD and Epithelial-mesenchymal transition (EMT) is the predominant stage of RF. β-catenin is a key component of the Wnt signaling pathway, which has been fully proven to promote EMT. However, the underlying mechanism of β-catenin in EMT during the pathogenesis of RF is yet to be determined.</div></div><div><h3>Objective</h3><div>This study was designed to investigate the effects of β-catenin on RF-related EMT and further investigate its underlying mechanism.</div></div><div><h3>Methods</h3><div>Human proximal tubular epithelial cell (HK-2) was treated with hypoxia to construct RF injury cell model. The viability of cells was determined by CCK-8 assay. Immunofluorescence was used to detect α-SMA content. Expressions of β-catenin, Brachyury and RF-related proteins were measured by Western blot. The correlation between β-catenin and Brachyury was detected by ChIP-qPCR and dual luciferase reporter assay.</div></div><div><h3>Results</h3><div>We found β-catenin was overexpressed in hypoxia-induced HK-2 cells. In the RF cell model, silencing of β-catenin weakened the EMT and fibrogenesis activity of HK-2 cells. Mechanistically, we found β-catenin binds to T-cell factor (TCF) to activate Brachyury, which is a positive player in EMT. Further studies clarified that Brachyury was responsible for β-catenin-promoted the EMT and HK-2 cell injury under hypoxia condition.</div></div><div><h3>Conclusions</h3><div>Herein, we demonstrated that β-catenin is overexpressed in hypoxia-induced HK-2 cells and promotes EMT and cell injury via activating Brachyury. These findings suggest that targeting β-catenin/Brachyury may be an effective new approach for treating RF.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101907"},"PeriodicalIF":2.3,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11741901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of platelet-rich plasma on ferroptosis of nucleus pulposus cells induced by Erastin","authors":"Shi-lin Lian ,&nbsp;Jie Huang ,&nbsp;Yan Zhang ,&nbsp;Yu Ding","doi":"10.1016/j.bbrep.2024.101900","DOIUrl":"10.1016/j.bbrep.2024.101900","url":null,"abstract":"<div><h3>Background</h3><div>Intervertebral disc degeneration (IVDD) has been linked to ferroptosis, a type of programmed cell death. The role of platelet-rich plasma (PRP) in mitigating ferroptosis in nucleus pulposus (NP) cells within IVDD remains unclear.</div></div><div><h3>Purpose</h3><div>This study aims to verify the effectiveness of PRP in reducing ferroptosis in NP cells induced by Erastin.</div></div><div><h3>Methods</h3><div>Primary NP cells were isolated from SD rats, and a ferroptosis model was established using Erastin. PRP was prepared and applied to assess its impact on ferroptosis-related markers, including reactive oxygen species (ROS), iron content, and glutathione peroxidase 4 (GPX4). The effects of PRP on the ultrastructure of NP cells were also observed using transmission electron microscopy (TEM).</div></div><div><h3>Results</h3><div>PRP treatment significantly restored GPX4 levels (431.47 ± 4.70 ng/L vs. 69.70 ± 4.06 ng/L, P &lt; 0.05), reduced ROS levels (45.06 ± 3.78 vs. 155.36 ± 3.56, P &lt; 0.05), and decreased iron content (32.24 ± 096 μg/L vs. 59.25 ± 3.72 μg/L, P &lt; 0.05) in ferroptotic NP cells compared to the sham group. Additionally, PRP significantly increased the expression levels of collagen Ⅱ (0.72 ± 0.02 vs. 0.33 ± 0.02, P &lt; 0.05) and aggrecan (0.81 ± 0.01 vs. 0.31 ± 0.02, P &lt; 0.05) compared to the sham group. TEM analysis also showed improvements in mitochondrial ultrastructure. These findings suggest that PRP can alleviate ferroptosis and promote cellular recovery.</div></div><div><h3>Conclusions</h3><div>The study demonstrates the potential of PRP as a therapeutic intervention in IVDD by mitigating ferroptosis in NP cells, offering a new theoretical basis for PRP treatment in degenerative disc diseases.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101900"},"PeriodicalIF":2.3,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heparin-binding of the human chitinase-like protein YKL-40 is allosterically modified by chitin oligosaccharides","authors":"Unnur Magnusdottir ,&nbsp;Finnbogi R. Thormodsson ,&nbsp;Lilja Kjalarsdottir ,&nbsp;Hordur Filippusson ,&nbsp;Johannes Gislason ,&nbsp;Kristinn Ragnar Oskarsson ,&nbsp;Jens G. Hjorleifsson ,&nbsp;Jon M. Einarsson","doi":"10.1016/j.bbrep.2024.101908","DOIUrl":"10.1016/j.bbrep.2024.101908","url":null,"abstract":"<div><div>The chitinase-like protein YKL-40 (CHI3L1) has been implicated in the pathophysiology of inflammation and cancer. Recent studies highlight the growing interest in targeting and blocking the activity of YKL-40 to treat cancer. Some of those targeting-strategies have been developed to directly block the heparin-affinity of YKL-40 with promising results. This study explores how short chain chitooligosaccharides (ChOS) affect the heparin-binding affinity of YKL-40. Our findings reveal that ChOS act as allosteric effectors, decreasing the heparin-binding affinity of YKL-40 in a size- and dose-dependent manner. Our results provide insights into the heparin affinity of YKL-40 and how ChOS can be used to target the heparin activity of YKL-40 in diseases. Since ChOS has many beneficial properties, such as being non-toxic and biodegradable, these results provide intriguing opportunities for applying them as allosteric effectors of the heparin-binding affinity of YKL-40.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101908"},"PeriodicalIF":2.3,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on metabolic disorders in rat liver induced by different times after scalds","authors":"Zhian Chen ,&nbsp;Hui Lin ,&nbsp;Xixiong Su ,&nbsp;Wenmang Xu ,&nbsp;Wei Fang ,&nbsp;Guangping Ruan ,&nbsp;Zhen Wang ,&nbsp;Guangchao Xu ,&nbsp;Rongqing Pang","doi":"10.1016/j.bbrep.2024.101904","DOIUrl":"10.1016/j.bbrep.2024.101904","url":null,"abstract":"<div><div>Previous studies have confirmed that burns and scalds can lead to metabolic disorders in the liver. However, the effects of severe burns at various time points on liver lipid metabolism disorders, as well as the relationship between these disorders and liver function, metabolism, and infection, have not yet been investigated.This study established a SD rat scald model, macroscopic observation of weight changes, histological staining, Western blot detection of fat browning and metabolic indicators, reverse transcription quantitative polymerase chain reaction analysis of the expression of liver new fat generation genes, determination of liver function and inflammatory indicators.The results show that steam scalding of 30 % of the back skin surface area of rats for 30, 20, and 10 s can result in severe skin scalds. Liver Oil Red O staining revealed fat deposition in the scald group, which became more pronounced with longer scald durations. The fat deposition was most evident on the fifth day post-scald and gradually returned to normal over time. This phenomenon is primarily attributed to elevated liver function indicators, including TBIL, ALT, and AST, in the scald group compared to the control group. Additionally, there was activation of peripheral blood inflammatory cells (WBC, MON, NEU,TNF-α, IL-6, and IL-10) and infiltration of inflammatory cells in the liver, along with liver cell edema. The honeycomb-like appearance of peripheral epididymal fat and the significant increase in the expression of lipolytic proteins (UCP1, ATGL, HSL, and P-HSL) were also observed, alongside abnormal expression of key genes (CES and SCD1) associated with liver neovascularization. The changes are caused by the combined effects of these factors.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101904"},"PeriodicalIF":2.3,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating combined hypoxia and stemness indices for prognostic transcripts in gastric cancer: Machine learning and network analysis approaches","authors":"Sharareh Mahmoudian-Hamedani ,&nbsp;Maryam Lotfi-Shahreza ,&nbsp;Parvaneh Nikpour","doi":"10.1016/j.bbrep.2024.101897","DOIUrl":"10.1016/j.bbrep.2024.101897","url":null,"abstract":"<div><h3>Introduction</h3><div>Gastric cancer (GC) is among the deadliest malignancies globally, characterized by hypoxia-driven pathways that promote cancer progression, including stemness mechanisms facilitating invasion and metastasis. This study aimed to develop a prognostic decision tree using genes implicated in hypoxia and stemness pathways to predict outcomes in GC patients.</div></div><div><h3>Materials and methods</h3><div>GC RNA-seq data from The Cancer Genome Atlas (TCGA) were analyzed to compute hypoxia and stemness scores using Gene Set Variation Analysis (GSVA) and the mRNA expression-based stemness index (mRNAsi). Hierarchical clustering identified clusters with distinct survival outcomes, and differentially expressed genes (DEGs) between clusters were identified. Weighted Gene Co-expression Network Analysis (WGCNA) identified modules and hub genes associated with clinical traits. Overlapping DEGs and hub genes underwent functional enrichment, protein-protein interaction (PPI) network analysis, and survival analysis. A prognostic decision tree was constructed using survival-associated shared genes.</div></div><div><h3>Results</h3><div>Hierarchical clustering identified six clusters among 375 TCGA GC patients, with significant survival differences between cluster 1 (low hypoxia, high stemness) and cluster 4 (high hypoxia, high stemness). Validation in the GSE62254 dataset corroborated these findings. WGCNA revealed modules linked to clinical traits and survival, with functional enrichment highlighting pathways like cell adhesion and calcium signaling. The decision tree, based on genes such as <em>AKAP6</em>, <em>GLRB</em>, and <em>RUNX1T1</em>, achieved an AUC of 0.81 (training) and 0.67 (test), demonstrating the utility of combined scores in patient stratification.</div></div><div><h3>Conclusion</h3><div>This study introduces a novel hypoxia-stemness-based prognostic decision tree for GC. The identified genes show promise as prognostic biomarkers, warranting further clinical validation.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101897"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The multifaceted role of SMAD4 in immune cell function","authors":"Xinmu Cui ,&nbsp;Yu Song ,&nbsp;Jianfeng Han ,&nbsp;Zhaoxin Yuan","doi":"10.1016/j.bbrep.2024.101902","DOIUrl":"10.1016/j.bbrep.2024.101902","url":null,"abstract":"<div><div>The Transforming Growth Factor-beta (TGF-β) signaling pathway, with SMAD4 as its central mediator, plays a pivotal role in regulating cellular functions, including growth, differentiation, apoptosis, and immune responses. While extensive research has elucidated SMAD4's role in tumorigenesis, its functions within immune cells remain underexplored. This review synthesizes current knowledge on SMAD4's diverse roles in various immune cells such as T cells, B cells, dendritic cells, and macrophages, highlighting its impact on immune homeostasis and pathogen response. Understanding SMAD4's role in immune cells is crucial, as its dysregulation can lead to autoimmune disorders, chronic inflammation, and immune deficiencies. The review emphasizes the significance of SMAD4 in immune regulation, proposing that deeper investigation could reveal novel therapeutic targets for immune-mediated conditions. Insights into SMAD4's involvement in processes like T cell differentiation, B cell class switch recombination, and macrophage polarization underscore its potential as a therapeutic target for a range of diseases, including autoimmune disorders and cancer.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101902"},"PeriodicalIF":2.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the prevalence of neutralizing antibodies (NAbs) to SARS-CoV-2 during three years of the COVID-19 pandemic","authors":"Marina dos Santos Barreto ,&nbsp;Ronaldy Santana Santos ,&nbsp;Eloia Emanuelly Dias Silva ,&nbsp;Deise Maria Rego Rodrigues Silva ,&nbsp;Pedro Henrique Macedo Moura ,&nbsp;Pamela Chaves de Jesus ,&nbsp;Jessiane Bispo de Souza ,&nbsp;Lucas Alves da Mota Santana ,&nbsp;Adriana Gibara Guimarães ,&nbsp;Lysandro Pinto Borges","doi":"10.1016/j.bbrep.2024.101903","DOIUrl":"10.1016/j.bbrep.2024.101903","url":null,"abstract":"<div><div>The development of COVID-19 vaccines has been an important step in the fight against the pandemic. However, it is still necessary to understand the influence of factors that can alter the immune response. In general, doses need to be updated frequently, and care must be taken to control the virus that is still circulating worldwide. In this study, we evaluated the neutralizing antibodies (NAbs) against SARS-CoV-2 in northeast Brazil. The study was divided into three phases (T1, T2, and T3) and included 297 participants. The three phases occurred in three different years of the pandemic (2021, 2022 and 2023). We obtained higher mean NAbs in T2 and T3 when most of the participants had already completed their vaccination program. No significant difference in the distribution of NAbs and sex was observed (p &gt; 0.05). It was shown that there is a difference in the expression of NAbs in the different amounts of doses, with individuals with no dose obtaining a significantly lower average than those who took the vaccine. Regarding age, the average NAbs were higher with increasing age. In this study, we assess the prevalence of NAbs in three pandemic phases, making it possible to understand the importance of vaccine updating in maintaining immunity.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101903"},"PeriodicalIF":2.3,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatic identification of signature miRNAs associated with fetoplacental vascular dysfunction in gestational diabetes mellitus","authors":"Yulan Lu ,&nbsp;Chunhong Liu ,&nbsp;Xiaoxia Pang ,&nbsp;Xinghong Chen ,&nbsp;Chunfang Wang ,&nbsp;Huatuo Huang","doi":"10.1016/j.bbrep.2024.101888","DOIUrl":"10.1016/j.bbrep.2024.101888","url":null,"abstract":"<div><h3>Background</h3><div>Intrauterine exposure to gestational diabetes mellitus (GDM) poses significant risks to fetal development and future metabolic health. Despite its clinical importance, the role of microRNAs (miRNAs) in fetoplacental vascular endothelial cell (VEC) programming in the context of GDM remains elusive. This study aims to identify signature miRNA genes involved in this process using bioinformatics analysis via multiple algorithms.</div></div><div><h3>Methods</h3><div>The dataset used in this study was acquired from Gene Expression Omnibus (GEO). Firstly, differentially expressed miRNA genes (DEMGs) were evaluated using limma package. Thereafter, an enrichment analysis of DEMGs was performed. Then, the least absolute shrinkage and selection operator (LASSO) and support vector machine (SVM) were used as the other algorithms for screening candidate signature miRNA genes. Genes from the intersection of limma, LASSO, and SVM genes were used as the final signature miRNA genes. The receiver operator characteristic curve (ROC), the nomogram diagram, gene set enrichment analysis (GSEA), and signature miRNAs-target genes interaction network were implemented further to explore the features and functions of signature genes.</div></div><div><h3>Results</h3><div>A total of 32 DEMGs, with 21 upregulated and 11 downregulated miRNA genes, were obtained from limma analysis. LASSO and SVM analyses identified 15 and 12 candidate signature miRNA genes, respectively. After the intersection of genes from limma, LASSO, and SVM analyses, MIR34A and MIR186 were found as the final signature genes related to fetoplacental VEC programming. MIR34A and MIR186 were highly expressed and were associated with an increased risk of fetoplacental VEC programming in GDM mothers. The area under the curve (AUC) of ROC for MIR34A and MIR186 were 0.960 and 0.935, respectively. GSEA analysis revealed that these signature genes positively participate in cellular processes related to VEC migration, cell differentiation, angiogenesis, programmed cell death, and inflammatory response. Finally, miRNAs-target genes interaction network analysis provides the interaction of signature miRNAs and their critical target genes, which may help further studies for miR-34a and miR-186 in GDM.</div></div><div><h3>Conclusions</h3><div>MIR34A and MIR186 are novel signature miRNA genes related to fetoplacental VEC programming that may represent critical genes associated with placental function and fetal programming under GDM conditions.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101888"},"PeriodicalIF":2.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11720096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectroscopic and calorimetric study of the interaction between Nile blue and double-stranded RNA","authors":"Md Dulal Hossain Khan,&nbsp;Ramya Ayyalasomayajula,&nbsp;Mare Cudic,&nbsp;Renjie Wang","doi":"10.1016/j.bbrep.2024.101899","DOIUrl":"10.1016/j.bbrep.2024.101899","url":null,"abstract":"<div><div>Nile blue has been widely used in histological staining, fluorescence labeling, and DNA probing, with its intercalation behavior into the DNA helix being well documented. Here, we present a comprehensive investigation to address a current knowledge gap regarding the binding properties of Nile blue to two types of double-stranded RNA (dsRNA): poly(A·U) and poly(I·C), using various biophysical techniques. Absorption and fluorescence spectroscopic studies suggest a significant binding interaction between Nile blue and the two designated dsRNAs, specifically indicating an intercalation binding mode with poly(A·U) and demonstrating a noticeably higher binding affinity compared to poly(I·C). The binding stoichiometry was further determined by Job's plot to be 0.47 for poly(A·U) and 1.0 for poly(I·C). The increased relative viscosity and changes in the circular dichroism (CD) ellipticity of dsRNA after interacting with Nile blue indicate the stacking of Nile blue dyes between the RNA duplexes. These changes suggest a conformational alteration of the dsRNAs and confirm the intercalation mode of binding. The thermal dynamic analysis demonstrates that both binding were favored by negative enthalpy and primarily driven by the hydrophobic effect.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101899"},"PeriodicalIF":2.3,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11714696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant expression and characterization of the family 5 cellulase from Bacillus velezensis in Escherichia coli BL21-CodonPlus (DE3)-RIPL","authors":"Dinh Minh Tran ,&nbsp;Thi Huyen Nguyen ,&nbsp;To Uyen Huynh ,&nbsp;Iuliia Pentekhina","doi":"10.1016/j.bbrep.2024.101898","DOIUrl":"10.1016/j.bbrep.2024.101898","url":null,"abstract":"<div><div>B. velezensis RB. IBE29 is a chitinolytic bacterium originally isolated from agricultural soil of Vietnam. Previous studies demonstrated this bacterium was a promising chitinase producer, biocontrol agent, and biofertilizer for crop production. The complete genome sequence of the bacterium was reported and possesses the gene encoding family 5 cellulase; however, role of this enzyme has not been experimentally characterized. This work aimed to express and biologically characterize family 5 cellulase of strain RB. IBE29. The ORF (without signal peptide) of the <em>celA</em> of strain RB. IBE29 was expressed in <em>E. coli</em> BL21-CodonPlus (DE3)-RIPL using the vector pColdII, and the corresponding product (rBvCelA, 55.17 kDa) was successfully purified using the HisTrap FF column. The purified rBvCelA showed the highest cellulase activity against CMC, followed by sugarcane bagasse and rice straw, and had optimal temperature and pH at 60 °C and 6.0, respectively. Metal salts ZnCl₂, FeCl₂, MgCl₂, CuSO₄, and MnCl₂ enhanced the cellulase activity by 103.85, 124.24, 109.38, 105.64, and 115.12 %, respectively. In addition, the supplementation of the purified rBvCelA in the feed enhanced the growth and improved the feed intake of broiler chickens by 5.88 and 5.19 %, respectively. These results indicated that family 5 cellulase of <em>B. velezensis</em> has a promising role in crop production and poultry breeding. As far as we know, this is the first report describing the contribution of family 5 cellulase from <em>B. velezensis</em> in broiler breeding.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101898"},"PeriodicalIF":2.3,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}