Essi Rytkönen , Janne Jänis , Anu Koivula , Juha Rouvinen
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引用次数: 0
Abstract
Unspecific peroxygenases (UPOs) are fungal enzymes capable of oxidizing hundreds of different organic compounds using hydrogen peroxide as the sole co-substrate. Aromatic ethers are among the less-studied substrates for UPOs, even though they offer a potential route to valuable phenolic compounds, such as those derived from the depolymerized lignin as a feedstock. Consequently, the oxyfunctionalisation potential of a panel of 30 UPO enzymes, along with Agrocybe aegerita UPO, was investigated using a simple aromatic ether, anisole. Anisole oxyfunctionalisation by the studied UPOs involved up to four consecutive reactions, resulting in the detection of 14 different products. Aromatic hydroxylation was the primary reaction pathway, leading to methoxyphenols or methoxybenzenediols. O-Demethylation also occurred, as phenol and benzenediols were identified among the products. As expected, different UPOs produced varying products and yields; however, guaiacol and 4-methoxyphenol were detected with every enzyme. The effect of ascorbic acid as a radical scavenger was also assessed, given that radical reactions leading to oligomerisation can occur with phenolic products. Generally, the presence of ascorbic acid broadened the product range, potentially due to the detection of trace products and a reduction in quinone formation. Owing to the broad product scope with anisole, biotransformations were also carried out with the identified phenolic intermediates to evaluate further oxidation routes. Based on these findings, a reaction pathway for anisole was proposed, offering insights into potential applications of UPOs in the tailored oxyfunctionalisation of phenolic substrates.
期刊介绍:
Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.