Biochemistry and Biophysics Reports最新文献_第6页

HUC-MSC-derived exosomal miR-16-5p attenuates inflammation via dual suppression of M1 macrophage polarization and Th1 differentiation huc - msc衍生的外泌体miR-16-5p通过双重抑制M1巨噬细胞极化和Th1分化来减轻炎症

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-09 DOI: 10.1016/j.bbrep.2025.102078

Yuanjing Zheng , Yue Li , Zhengyang Wei , Yang Wang , Yuanlin Liu , Fengsong Liu , Xue Li , Yi Zhang

{"title":"HUC-MSC-derived exosomal miR-16-5p attenuates inflammation via dual suppression of M1 macrophage polarization and Th1 differentiation","authors":"Yuanjing Zheng , Yue Li , Zhengyang Wei , Yang Wang , Yuanlin Liu , Fengsong Liu , Xue Li , Yi Zhang","doi":"10.1016/j.bbrep.2025.102078","DOIUrl":"10.1016/j.bbrep.2025.102078","url":null,"abstract":"<div><div>Nowadays mesenchymal stem cell-derived exosomes (MSC-Exos) have emerged as a promising cell-free therapeutic alternative to MSC-based therapies, demonstrating efficacy in treating degenerative diseases, inflammatory disorders, and autoimmune diseases. MSC-Exos transport bioactive cargoes such as proteins, lipids, mRNAs, and microRNAs (miRNAs) to the recipient cells, mediating intercellular communication to regulate immunomodulation and tissue repair. However, the exosomal miRNA profile varies dynamically based on the culture conditions and tissue sources. Thus, elucidating the specific exosomal miRNA profile and regulatory targets is critical for the precise clinical applications and development of MSC-Exos-based cell-free therapies.</div><div>Here we established an optimized serum-free culture system for human umbilical cord-derived MSCs (hUC-MSCs) and determined the critical 48–72-h harvest window for exosome secretion. High-throughput sequencing identified miR-16-5p as the predominant exosomal miRNA, functioning as a core immunosuppressive effector by suppressing LPS/IFN-γ-induced M1 macrophage polarization and Th1 cell differentiation. Mechanistically, miR-16-5p was found to target key nodes in NF-κB and JAK-STAT pathways, validated via dual-luciferase assays. Additionally, miR-125b-5p and miR-34a-5p enhanced this immunosuppressive effect by co-targeting overlapping pathway components in NF-κB and JAK-STAT pathways, suggesting a multilayered regulatory network. Taken together, our findings highlight the potential of miRNA-engineered exosomes as standardized therapies for inflammatory disorders, emphasizing the importance of optimizing culture conditions and profiling miRNA expression over time in advancing clinical translation.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102078"},"PeriodicalIF":2.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Combination of AURKA inhibitor and MEK inhibitor strongly enhances G1 arrest and induces synergistic antitumor effect on KRAS or BRAF mutant colon cancer cells AURKA抑制剂和MEK抑制剂联合使用可增强G1阻滞，诱导KRAS或BRAF突变结肠癌细胞的协同抗肿瘤作用

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-09 DOI: 10.1016/j.bbrep.2025.102073

Masashi Sato , Yoshiyuki Yamamoto , Toshikazu Moriwaki , Kuniaki Fukuda , Kiichiro Tsuchiya

{"title":"Combination of AURKA inhibitor and MEK inhibitor strongly enhances G1 arrest and induces synergistic antitumor effect on KRAS or BRAF mutant colon cancer cells","authors":"Masashi Sato , Yoshiyuki Yamamoto , Toshikazu Moriwaki , Kuniaki Fukuda , Kiichiro Tsuchiya","doi":"10.1016/j.bbrep.2025.102073","DOIUrl":"10.1016/j.bbrep.2025.102073","url":null,"abstract":"<div><div>In colorectal cancer, <em>RAS</em> and <em>BRAF</em> are major mutation points in the RAS-MAPK signaling pathway. These gene mutations are known to be important causes of resistance to anti-EGFR antibody therapies. Recently, it has been reported that Aurora kinase A (AURKA), one of the mitotic kinases, interacts with the EGFR-RAS-MAPK signaling pathway. In this study, we examined whether the combination of MK-5108 (AURKA inhibitor) and trametinib (MEK inhibitor) enhanced the antitumor effect for colon cancer cell lines. The combination of MK-5108 and trametinib showed synergistic enhancements of antitumor effect in three colon cancer cell lines harboring <em>KRAS</em> or <em>BRAF</em> mutation. Cell cycle analysis showed induction of G2/M and G1 arrests by MK-5108 and trametinib, respectively, and the potential enhancement of G1 arrest with the two drug combination. The addition of MK-5108 to trametinib enhanced the suppression of <em>p</em>-ERK and other G1/S progression-related proteins expression. In HCT116 cells, harboring wild-type <em>TP53</em>, the combination therapy induced more potent cell proliferation suppression and apoptosis induction than in <em>TP53</em> knockout cells. These were related to potential enhancement of p53 expression and caspase activation. In conclusion, the combination of MK-5108 and trametinib may synergistically inhibit tumor cell division with or without <em>TP53</em> mutation, and with either <em>KRAS</em> or <em>BRAF</em> mutation. Furthermore, the combination therapy could be more effective in wild-type <em>TP53</em> cells.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102073"},"PeriodicalIF":2.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Blood perfusion through ventricular assist devices induces erythrocytes to interact with leukocytes 血液灌注通过心室辅助装置诱导红细胞与白细胞相互作用

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-08 DOI: 10.1016/j.bbrep.2025.102085

Kylie M. Foster , Ahmed M. El Banayosy , Zheila Azartash-Namin , Phillip Coghill , James W. Long , Edgar O'Rear , Hendra Setiadi

{"title":"Blood perfusion through ventricular assist devices induces erythrocytes to interact with leukocytes","authors":"Kylie M. Foster , Ahmed M. El Banayosy , Zheila Azartash-Namin , Phillip Coghill , James W. Long , Edgar O'Rear , Hendra Setiadi","doi":"10.1016/j.bbrep.2025.102085","DOIUrl":"10.1016/j.bbrep.2025.102085","url":null,"abstract":"<div><div>Implantations of Ventricular Assist Devices (VADs) have significantly improved quality of life and life expectancy of end-stage heart failure patients. However, despite the advancements in the VAD designs and patient management protocols, the VAD recipients remain at risk of attaining bleeding, infection, pump thrombosis, and stroke. Although blood trauma has been suggested as a critical factor in development of these adverse events, its consequences in inducing interactions between different types of blood cells are largely unknown.</div><div>Following our recent findings on erythrocyte and leukocyte trauma in VAD recipients, we decided to explore interactions between erythrocytes and leukocytes by perfusing human whole blood through two different types of VADs, HeartMate II (HMII) and HeartMate 3 (HM3), concurrently in their respective Blood Circulatory Loop (BCL). By using a flow cytometry assay, we found increasing association between erythrocytes and leukocytes as VADs propelled blood through their BCLs. This time-dependent intercellular association was shown by increasing concomitant events of stained CD235a (specifically expressed on erythrocytes) and stained CD45 (specifically expressed on leukocytes) in the CD235a<sup>+</sup> population. Compared to CentriMag (CM), which served as a control, VADs produced significantly higher concomitant signals. The findings described in this study have opened the need for further studies on a novel path for generation of adverse events that are commonly observed in VAD recipients, notably infection, pump thrombosis, and ischemic stroke.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102085"},"PeriodicalIF":2.3,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Cytoplasmic physicochemical factors drive malignant transformation by adapting bioenergetic settings 细胞质物理化学因素驱动恶性转化通过适应生物能量设置

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Biochemistry and Biophysics Reports Pub Date : 2025-06-07 DOI: 10.1016/j.bbrep.2025.102079

Tattym E. Shaiken

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Engineering bacterial cell morphology for the design of robust cell factories 工程细菌细胞形态设计稳健的细胞工厂

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-07 DOI: 10.1016/j.bbrep.2025.102076

Maarten Lubbers, Nova Jaspers, Dennis Claessen

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Novel and efficient synthesis of 5-chloro-6-methoxy-3-(2-((1-(aryl)-1H-1,2,3-triazol-4-yl)methoxy)ethyl)benzo[d]isoxazole derivatives as new α-glucosidase inhibitors 新型α-葡萄糖苷酶抑制剂5-氯-6-甲氧基-3-（2-（1-（芳基））- 1h -1,2,3-三唑-4-基）甲氧基)乙基)苯并[d]异恶唑衍生物的高效合成

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-05 DOI: 10.1016/j.bbrep.2025.102074

Ram Reddy Mudireddy , Rambabu Gundla , Chandra Prakash Koraboina , Vani Madhuri Velavalapalli , Venkata Veernjaneya Sarma Dhulipalla , Gowri Sankararao Burle , Sreekantha B. Jonnalagadda , Naresh Kumar Katari

{"title":"Novel and efficient synthesis of 5-chloro-6-methoxy-3-(2-((1-(aryl)-1H-1,2,3-triazol-4-yl)methoxy)ethyl)benzo[d]isoxazole derivatives as new α-glucosidase inhibitors","authors":"Ram Reddy Mudireddy , Rambabu Gundla , Chandra Prakash Koraboina , Vani Madhuri Velavalapalli , Venkata Veernjaneya Sarma Dhulipalla , Gowri Sankararao Burle , Sreekantha B. Jonnalagadda , Naresh Kumar Katari","doi":"10.1016/j.bbrep.2025.102074","DOIUrl":"10.1016/j.bbrep.2025.102074","url":null,"abstract":"<div><div>A new series of benzisoxazole derivatives (<strong>9a-o</strong>) were designed by using molecular hybridization approach and synthesized <em>via</em> click-chemistry. All the synthesized compounds were evaluated for their α-glucosidase enzyme inhibition and antibacterial activity. All tested compounds (<strong>9a-o</strong>) exhibited a promising α-glucosidase inhibitory activity with IC<sub>50</sub> range of 14.69–38.71 nmol in comparison with the positive drug <strong>Acarbose</strong> (IC<sub>50</sub> 35.91 nmol). Additionally, these compounds have found to be active against <em>B. cereus</em> and <em>E. coli</em>. The <em>in vitro</em> inhibition results supported to <em>in silico</em>. Additionally, the compounds were subjected to computational drug-likeness/ADME testing, which revealed that this all the compounds had good ADME profiles in addition to exhibiting drug-like qualities. SAR indicates that analysis revealed that electron-withdrawing substituents such as Br and CF<sub>3</sub> at specific positions significantly enhanced α-glucosidase inhibition, while unsubstituted and ortho-methoxy phenyl derivatives also showed potent activity, highlighting the benzo[d]isoxazole–triazole scaffold as a promising pharmacophore for developing novel anti-diabetic agents.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102074"},"PeriodicalIF":2.3,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Isolation, biochemical characterization, and primary structure and active site determination of Dioscorea opposita (‘Nagaimo’) oligopeptidase B 薯蓣寡肽酶B的分离、生化鉴定、一级结构和活性位点测定

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-04 DOI: 10.1016/j.bbrep.2025.102071

Sayaka Miyazaki-Katamura , Mami Chosei , Sota Tate , Tomohisa Sakaue , Takuya Yamane , Junko Suzuki , Shigeki Higashiyama , Iwao Ohkubo

{"title":"Isolation, biochemical characterization, and primary structure and active site determination of Dioscorea opposita (‘Nagaimo’) oligopeptidase B","authors":"Sayaka Miyazaki-Katamura , Mami Chosei , Sota Tate , Tomohisa Sakaue , Takuya Yamane , Junko Suzuki , Shigeki Higashiyama , Iwao Ohkubo","doi":"10.1016/j.bbrep.2025.102071","DOIUrl":"10.1016/j.bbrep.2025.102071","url":null,"abstract":"<div><div>A protease was purified to homogeneity from <em>Dioscorea opposita “</em>Nagaimo” using ion exchange, hydrophobic and gel filtration columns, and its biochemical characterization including molecular weight, substrate specificity and kinetic parameters were determined. Protease activity was strongly inhibited by AEBSF, DCI and TLCK. The enzyme moderately inhibited by NEM and HgCl<sub>2</sub>. The enzyme activity inhibited by NEM and HgCl<sub>2</sub> was restored with the addition of β-ME. These findings suggest that the enzyme is a trypsin-like serine protease, which is regulated by SH compounds. The N-terminal amino acid of this protease is blocked in an unknown manner. We determined the structure of the cDNA and deduced amino acid sequence of the protease from <em>D. opposita</em>. The cDNA was composed of 2420 nucleotides and encoded 751 amino acids in the coding region. The results indicated that this enzyme is an oligopeptidase B (OPB), consisting of a N-terminal region (M<sup>1</sup> ∼ T4<sup>7</sup>), a N-terminal β-propeller domain (A<sup>48</sup>∼ L<sup>465</sup>), a connecting domain (K<sup>466</sup> ∼ D<sup>527</sup>), a peptidase_S9 domain (P<sup>528</sup> ∼ D<sup>744</sup>) and C-terminal region (R<sup>745</sup> ∼ S<sup>751</sup>). The overall homology of amino acid sequences of <em>D. opposita</em> to <em>D. alata</em> and <em>D. rotundata</em> was 99.07 % and 97.07 %, respectively. The catalytically active amino acid sites [S<sup>599</sup>, D<sup>684</sup>, and H<sup>719</sup>] among these yam species were found to be highly conserved. Site-directed mutagenesis confirmed that these three the active center.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102071"},"PeriodicalIF":2.3,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Effects of carminic acid on gene expressions under epigenetic regulation carminic acid对表观遗传调控下基因表达的影响

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-03 DOI: 10.1016/j.bbrep.2025.102070

Kei-ichi Sugiyama , Petr Grúz , Kaoru Sato , Masamitsu Honma

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Meta-analysis study of the therapeutic impact of Mesenchymal stem cells derived exosomes for chronic kidney diseases 间充质干细胞衍生外泌体治疗慢性肾脏疾病的meta分析研究

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-03 DOI: 10.1016/j.bbrep.2025.102072

Himanshu , Gunjan , Ramendera Pati Pandey , Riya Mukherjee , Chung-Ming Chang

{"title":"Meta-analysis study of the therapeutic impact of Mesenchymal stem cells derived exosomes for chronic kidney diseases","authors":"Himanshu , Gunjan , Ramendera Pati Pandey , Riya Mukherjee , Chung-Ming Chang","doi":"10.1016/j.bbrep.2025.102072","DOIUrl":"10.1016/j.bbrep.2025.102072","url":null,"abstract":"<div><div>Mesenchymal stem cell-derived exosomes (EXOs) represent a promising avenue for treating chronic kidney diseases (CKD), though their precise impact remains somewhat elusive. To address this gap, we conducted a systematic analysis, scouring databases and clinical trial repositories for relevant studies from 2019 to 2023. Seventeen papers were meticulously selected for their focus on mesenchymal stem cell-derived exosomes (MSC-EXOs) and their potential in CKD treatment. Our comprehensive meta-analysis, incorporating 15 preclinical and 6 clinical studies, underscores the efficacy of MSC-EXOs in improving renal function while attenuating tubular injury, inflammation, apoptosis, collagen deposition, and renal fibrosis. Notably, post-treatment with MSC-EXOs exhibited significant associations with various CKD markers, with pooled proportions indicating a considerable impact on blood urea nitrogen (BUN) and serum creatinine (SCR) levels. Subgroup analyses based on animal models further elucidated heterogeneity within the studies. In conclusion, MSC-EXOs demonstrate promise in enhancing renal function and reducing CKD risk, as evidenced by both preclinical and clinical data. Their efficacy in lowering SCR and BUN levels while enhancing filtration rate suggests MSC-EXOs as a viable and secure alternative to cell-based therapies, thereby providing valuable insights for personalized CKD treatments despite inherent limitations.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102072"},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Fundamental mechanisms of cell death for polycystic ovary syndrome 多囊卵巢综合征细胞死亡的基本机制

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-03 DOI: 10.1016/j.bbrep.2025.102043

Ying-ying Li, Yi-qiu Peng, Yu-xi Yang, Ning Xu, Ting-juan Shi, Rui-xia Liu, Ying-yi Luan, Cheng-hong Yin

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