Biochemistry and Biophysics Reports最新文献

{"title":"Role of Galanin system and insulin resistance parameters as predictive tools for diagnosis of Long-COVID patients","authors":"Wasim Talib Al Masoodi ,&nbsp;Sami Waheed Radhi ,&nbsp;Habiba Khdair Abdalsada ,&nbsp;Hussein Kadhem Al-Hakeim","doi":"10.1016/j.bbrep.2025.102068","DOIUrl":"10.1016/j.bbrep.2025.102068","url":null,"abstract":"<div><h3>Background</h3><div>COVID-19 patients may have long-lasting symptoms known as long-COVID (LC) without any underlying medical issues or obvious organ damage. Much research suggested that these issues are attributed to cytokine storm, lung and nerve injury, and glucose homeostasis disruption. Galanin (Gal), a neuropeptide in the peripheral and central nervous systems, has several physiological activities connected to illnesses. The current case-control research hypothesized the role of insulin resistance (IR) and the Gal system in LC pathophysiology.</div></div><div><h3>Methods</h3><div>This research included 30 healthy controls and 60 LC patients. Insulin, Gal, and GalR1 were determined using the enzyme-linked immunosorbent assay (ELISA). The HOMA2 calculator determined β-cell function (HOMA%B), insulin sensitivity (HOMA%S), and insulin resistance (HOMA2IR) by analyzing fasting serum insulin and glucose levels.</div></div><div><h3>Results</h3><div>LC patients showed higher Gal, GalR1, and Gal/GalR1 concentrations than controls, suggesting Gal system activation. LC patients likely have an IR state. The correlation study showed a negative link between Gal, GalR1, and SpO2. Gal level was positively correlated with insulin, insulin/glucose, and HOMA2IR and negatively correlated with HOMA%S. With an AUC-ROC of 0.939, artificial neural networks (ANN) predicted a sensitivity of 71.4 % and a specificity of 87.5 %. In LC, IR parameters and Gal system biomarkers were strongly correlated, suggesting they may contribute to disease.</div></div><div><h3>Conclusion</h3><div>Galanin system and IR parameters are altered in LC patients and can predict LC in suspicious subjects with 91.7 % sensitivity and 100.0 % specificity using the neural network model. The top five predictors were CRP, insulin/glucose, Gal, glucose, and GalR1. CRP had the greatest importance (100.0 %), indicating the importance of inflammation, IR, and Gal system biomarkers in the pathophysiology of LC.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102068"},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcranial electrical stimulation (TES) in human motor Optimization: Mechanisms, safety, and emerging applications","authors":"Jingfeng Wang ,&nbsp;Li Wu ,&nbsp;Mingming Sun ,&nbsp;Yuxiang Wu","doi":"10.1016/j.bbrep.2025.102055","DOIUrl":"10.1016/j.bbrep.2025.102055","url":null,"abstract":"<div><div>Non-invasive brain stimulation (NIBS) has emerged as a rapidly advancing field, offering promising therapeutic interventions for a range of neurological disorders while effectively bridging the gap between laboratory research and clinical applications. Among NIBS technologies, transcranial electrical stimulation (TES) stands out as a notable example, utilizing electrodes of varying sizes to deliver low-intensity electrical currents to specific regions of the cerebral cortex. This technique facilitates the modulation of neuronal excitability, regulation of brainwave activity, promotion of neural remodeling and repair, enhancement of cerebral blood flow, and improvement of brain-muscle connectivity. Despite its potential, current research on the effects of TES on motor function across diverse populations, particularly from a central nervous system perspective, remains limited. This review seeks to establish a theoretical framework for the future advancement of TES technology in sports science, elucidate the neurophysiological mechanisms underlying various TES modalities, and synthesize the most recent experimental findings from the past two decades regarding its impact on physical fitness, motor skill acquisition, and recovery in different populations.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102055"},"PeriodicalIF":2.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of oxidative stress in prediabetes development","authors":"Aarthi Yesupatham,&nbsp;Radha Saraswathy","doi":"10.1016/j.bbrep.2025.102069","DOIUrl":"10.1016/j.bbrep.2025.102069","url":null,"abstract":"<div><div>An imbalance between the generation of reactive oxygen species (ROS) and antioxidant defense systems is known as oxidative stress, and it is a major factor in diseases like atherosclerosis, vascular inflammation, endothelial dysfunction, and others that are largely caused by elevated oxidative stress. Interestingly, oxidative stress has also been implicated in the progression of prediabetes to type 2 diabetes (T2DM). Prediabetes, characterized by increased blood glucose levels than the diabetes threshold, impacts a substantial portion of the global population. In India alone, the estimated prevalence of prediabetes by 2023 is approximately 15.3 %. Hyperglycaemia, a hallmark of prediabetes, can promote vascular dysfunction by increasing ROS formation and upregulating chronic inflammatory markers. Additionally, oxidative stress contributes to insulin resistance and impaired beta cell function. Various risk factors of oxidative stress are associated with prediabetes development. This review focuses on ROS's role in prediabetes pathogenesis and its risk factors increasing the effect of oxidative stress. Various ROS scavengers were used as oxidative stress indicators, and ROS scavenging has a deleterious impact on disease progression. Lack of study has reported on role of oxidative stress in prediabetes development and this will be the first review covers pathogenesis and impact of oxidative stress biomarker in prediabetes. This review comprises managing the risk factors of oxidative stress would pave the way for the management of prediabetes progression to T2DM.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102069"},"PeriodicalIF":2.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induced volatolomics to uncover new enzymatic hallmarks of precancerous lesions: a proof of concept on gastric preneoplasia in mice","authors":"Rony Eid ,&nbsp;Estelle Blochouse ,&nbsp;Alban Giese ,&nbsp;Sébastien Papot ,&nbsp;Philippe Jay ,&nbsp;Christine Varon ,&nbsp;Pauline Poinot","doi":"10.1016/j.bbrep.2025.102062","DOIUrl":"10.1016/j.bbrep.2025.102062","url":null,"abstract":"<div><div>Induced volatolomics is an emerging field of research that offers new opportunities in biology by detecting volatile reporters released by activatable probes, enabling the exploration of oncogenic processes. Building on its proven efficiency in exploring the evolution of implanted tumours, we hypothesized that induced volatolomics could extend its application to detect precancerous conditions. As a proof of concept, we performed a longitudinal study and investigated glycosidase activity during the early stages of gastric carcinogenesis development induced by <em>Helicobacter felis</em> infection in mice, mimicking the gastric carcinogenesis cascade induced by chronic <em>Helicobacter pylori</em> infection in humans. We identified upregulated exoglycosidases linked to acute infections or inflammatory processes in tissues infected by Helicobacter. Specifically, α-mannosidase and β-galactosidase activities in stomach tissue were found to be strongly associated with the initial stages of <em>Helicobacter</em> infection. Additionally, the activities of β-Glucuronidase and β-N-acetyl-glucosaminidase increased during the progression to preneoplastic stages, potentially signalling the transition from infection to inflammation-driven carcinogenesis. These enzymes may serve as early biomarkers for detecting gastric carcinogenesis. Our study highlights the potential of VOC-based probes for real-time monitoring of gastric cancer progression through tissue biopsies. Therefore, this study demonstrates the potential of induced volatolomics for investigating biological processes and uncovering new therapeutic strategies.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102062"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary spectroscopic studies of the interactions between 9-fluoro-5-alkyl-12(H)-quino[3,4-b][1,4]benzothiazine chloride and DNA","authors":"Aleksandra Owczarzy ,&nbsp;Wojciech Rogóż ,&nbsp;Karolina Kulig ,&nbsp;Andrzej Zięba ,&nbsp;Małgorzata Maciążek-Jurczyk","doi":"10.1016/j.bbrep.2025.102067","DOIUrl":"10.1016/j.bbrep.2025.102067","url":null,"abstract":"<div><div>Cancer treatment is one of the challenges of modern medicine. The advancement of new anticancer drugs requires a comprehensive understanding of their mechanism of action and structure-activity relationships. 9-fluoro-5-alkyl-12(H)-quino[3,4-b][1,4]benzothiazine chloride (Salt2) is a newly synthesized substance exhibiting promising anticancer activity. Deoxyribonucleic acid (DNA) is a macromolecule of high biological importance. DNA is essential for cellular development, including DNA replication, transcription and translation. Binding of small molecules to DNA can inhibit or modify cellular DNA function and induces cell death. In turn, it can allow to alleviate or control the disease.</div><div>The objective of the research was to assess the interactions of a newly developed substance (Salt2) and calf thymus DNA (ctDNA) using UV-VIS, spectrofluorescence and circular dichroism spectroscopy. The UV-VIS and fluorescence analysis showed that Salt2 might form a strong, intercalative complex in the ground state with ctDNA.</div><div>Due to the fact that Salt2 changes ctDNA structure it can be assumed, that Salt2 can effect on DNA replication, transcription and translation processes and inhibits or modifies cellular DNA function. The results obtained for the Salt2-ctDNA complex, not only encourage further research, but may also prove useful in determining binding to human cellular DNA.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102067"},"PeriodicalIF":2.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the vestibulo-ocular reflex in head-tilt mutant mice","authors":"Shotaro Harada ,&nbsp;Yoshihisa Koyama ,&nbsp;Takao Imai ,&nbsp;Yasumitsu Takimoto ,&nbsp;Hidenori Inohara ,&nbsp;Shoichi Shimada","doi":"10.1016/j.bbrep.2025.102066","DOIUrl":"10.1016/j.bbrep.2025.102066","url":null,"abstract":"<div><div>The vestibulo-ocular reflex (VOR) is an involuntary reflex essential for maintaining clear vision and balance during head movement. It stabilizes gaze by generating compensatory eye movements in the opposite direction to head movement, ensuring that the visual image is stable on the retina. Maintaining visual stability leads to enhanced postural control and balance, which are crucial for coordinating daily activities, such as exercise and driving. The two types of balance organs, semicircular canals and otolith organs, are involved in the VOR; however, how they interact to contribute to the VOR remains unclear. This study aimed to investigate the interrelationship between semicircular canals and otolith organs in terms of balance function by performing semicircular canal and otolith organ function tests using head-tilt (Het) mutant mice that lack otoconia. Linear VOR results indicated that eye movements induced by linear acceleration stimuli were not observed in Het mutant mice, regardless of the degree of gravitational acceleration. Angular VOR was observed in Het mutant mice at 1 and 2 Hz, but a reduced VOR gain was observed at 0.3 Hz. In wild-type mice, both the linear and angular VOR were normal. The present study demonstrated that in Het mutant mice, the angular VOR decreased after 0.3-Hz semicircular canal stimulation, but normal responses were observed after 1- and 2-Hz semicircular canal stimulation. These results suggest that otolith organs are closely involved in the angular VOR during slow rotational stimulation.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102066"},"PeriodicalIF":2.3,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing carbonate mineral-forming bacterial strains and their carbonic anhydrase activities in two coastal sabkhas","authors":"Khaled Naja ,&nbsp;Sara H. Alhadidi ,&nbsp;Hadil Elsayed ,&nbsp;Jassim Abdulla A. Al-Khayat ,&nbsp;Fadhil Sadooni ,&nbsp;Hamad Al Saad Al-Kuwari ,&nbsp;Zulfa Ali Al Disi","doi":"10.1016/j.bbrep.2025.102064","DOIUrl":"10.1016/j.bbrep.2025.102064","url":null,"abstract":"<div><div>The enzyme carbonic anhydrase (CA) plays a key role in carbonate mineral formation by facilitating the interconversion between CO₂ and bicarbonate ions, thus influencing carbonate precipitation processes in natural environments. This study investigates the biomineralization potential of <em>Virgibacillus</em> strains isolated from two distinct coastal sabkhas in Qatar—Dohat Faishakh Sabkha (DFS) and Khor Al-Adaid Sabkha (KAS)—to better understand the enzymatic mechanisms driving carbonate formation in hypersaline environments. The isolated strains were evaluated for mineral formation and CA activity using three artificial media designed to simulate natural conditions: MD1, seawater-based with tryptone (SWTr), and evaporated seawater-based with tryptone (EWTr). While all strains demonstrated the ability to form minerals in MD1, only <em>Virgibacillus salarius</em> and <em>Virgibacillus marismortui</em>, both exclusive to DFS, exhibited robust mineral precipitation in SWTr and EWTr media. These strains also showed significantly higher CA activity compared to <em>Virgibacillus chiguensis</em> and <em>Virgibacillus dokdonensis</em>, which were present in both sabkhas but displayed limited mineralization and low enzymatic activity under saline conditions.</div><div>Statistical analyses, including ANOVA and principal component analysis (PCA), confirmed the significant role of CA activity and salinity in modulating biomineralization potential among these strains. This research underscores the potential of CA-driven biomineralization for environmental applications. The ability of <em>V. salarius</em> and <em>V. marismortui</em> to precipitate carbonates under high-salinity conditions positions them as promising candidates for bio-based carbon sequestration technologies.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102064"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interplay between tunneling nanotubes and Wnt Signaling: Insights into cytoskeletal regulation and therapeutic potential","authors":"Tengfei Feng ,&nbsp;Qi Xu ,&nbsp;Shuangshuang Wang ,&nbsp;Dongyu Hou ,&nbsp;Xunwei Wu","doi":"10.1016/j.bbrep.2025.102065","DOIUrl":"10.1016/j.bbrep.2025.102065","url":null,"abstract":"<div><div>Tunneling nanotubes (TNTs) are membranous structures that enable direct intercellular transfer of mitochondria, proteins, RNAs, and signaling molecules, playing key roles in tissue repair, immune coordination, and stress adaptation. Among their critical functions, TNT-mediated mitochondrial transfer rescues metabolically impaired cells, yet the regulatory mechanisms governing TNT formation and function remain incompletely understood. Recent studies highlight the Wnt signaling pathway—a conserved regulator of cell fate, polarity, and cytoskeletal remodeling—as a central modulator of TNT dynamics. Through its canonical (Wnt/β-catenin) and non-canonical (Wnt/PCP and Wnt/Ca²⁺) branches, Wnt signaling orchestrates actin filament organization, bundling, and turnover, all of which are essential for TNT biogenesis and stability. This review critically examines the mechanistic intersection between Wnt signaling and TNTs, with an emphasis on how Wnt-driven cytoskeletal remodeling supports intercellular connectivity. Beyond basic mechanistic insights, we also explore the physiological and pathological relevance of this crosstalk—including its roles in tissue regeneration, immune modulation, cancer progression, and neurodegeneration. While the Wnt–TNT axis offers therapeutic promise, its context-dependent effects demand careful consideration.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102065"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive risk model of disulfidoptosis-related lncRNAs predicts prognosis and therapeutic implications in bladder cancer","authors":"Zhixiong Zhang ,&nbsp;Jinghua Zhong ,&nbsp;Muhammad Sarfaraz Iqbal ,&nbsp;Zhiwen Zeng ,&nbsp;Xiaolu Duan","doi":"10.1016/j.bbrep.2025.102060","DOIUrl":"10.1016/j.bbrep.2025.102060","url":null,"abstract":"<div><h3>Background</h3><div>Disulfidoptosis is an emerging form of regulated cell death; however, the roles of its associated long non-coding RNAs (dr-lncRNAs) in bladder cancer (BLCA) remain poorly characterized. By leveraging the most comprehensive curated dataset of disulfidoptosis-related genes to date, we systematically developed and validated a novel dr-lncRNA signature that elucidates the prognostic significance and immune microenvironmental dynamics in BLCA.</div></div><div><h3>Methods</h3><div>The Cancer Genome Atlas (TCGA) database was utilized to extract significant clinical and RNA sequencing data of BLCA patients. Cox and Lasso regression with several variables was used to create a risk model. ROC, Kaplan-Meier, and nomogram analyses were carefully reviewed for validity. The validated study evaluated intricate interactions between functional enrichment, immune cell infiltration, cancer mutation load, and treatment sensitivity. Unsupervised consensus clustering identified subgroup patterns that reflected immune system alterations, medication susceptibility, and prognosis.</div></div><div><h3>Results</h3><div>Nine lncRNAs significantly correlated with prognosis were collectively identified, subsequently forming the basis for constructing a risk model consisting of seven lncRNAs. The model exhibited significant superiority in predicting patient outcomes, effectively distinguishing between high-risk from low-risk individuals. Functional enrichment analysis uncovered their potential involvement in immune-related biological pathways. Patients in the high-risk group exhibited higher tumor mutation burdens, more active immune functions and a higher sensitivity to chemotherapeutic drugs. Variations among BLCA subgroups were identified by consensus cluster analysis, including clinical characteristics, prognosis, lncRNA expression, immune cell infiltration, and immune checkpoint profiles.</div></div><div><h3>Conclusion</h3><div>The dr-lncRNAs-based risk model presents a promising tool for predicting prognosis and guiding personalized immunotherapy and treatment strategies in BLCA patients.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"42 ","pages":"Article 102060"},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spinal cord injury models: Advantages and disadvantages in the view of pathophysiology and clinical significance","authors":"Min-Qi Li ,&nbsp;Qing-Hua Wang ,&nbsp;Chuan-Ming Dong ,&nbsp;Long-Ju Qi","doi":"10.1016/j.bbrep.2025.102063","DOIUrl":"10.1016/j.bbrep.2025.102063","url":null,"abstract":"<div><div>Over recent decades, SCI research has advanced in understanding its pathophysiology and related mechanisms. Researchers developed many rodent SCI models to mimic injury processes, which are crucial for evaluating therapies and understanding pathology. But choosing the right model for specific research is challenging due to distinct pathological changes. This review overviews SCI pathophysiology and examines rodent models. It emphasizes model - related challenges, application value, simulation degrees, and replication of pathologies. It also discusses model advantages and limitations, and references recent reverse-engineering projects, which provide a brand-new perspective for promoting SCI research.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"42 ","pages":"Article 102063"},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144139339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}