诱导挥发组学揭示癌前病变的新酶标记:小鼠胃瘤前病变的概念证明

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Rony Eid , Estelle Blochouse , Alban Giese , Sébastien Papot , Philippe Jay , Christine Varon , Pauline Poinot
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引用次数: 0

摘要

诱导挥发组学是一个新兴的研究领域,通过检测可激活探针释放的挥发性报告因子,从而探索致癌过程,为生物学提供了新的机会。基于其在探索植入肿瘤进化方面的有效性,我们假设诱导挥发组学可以扩展其在检测癌前病变方面的应用。为了证明这一概念,我们进行了一项纵向研究,模拟人类慢性幽门螺杆菌感染引起的胃癌级联反应,研究了猫幽门螺杆菌感染诱导小鼠胃癌发生早期阶段的糖苷酶活性。我们发现,在被幽门螺杆菌感染的组织中,外糖苷酶的上调与急性感染或炎症过程有关。具体来说,胃组织中α-甘露糖苷酶和β-半乳糖苷酶活性与幽门螺杆菌感染的初始阶段密切相关。此外,β-葡糖苷酶和β- n-乙酰-葡糖苷酶的活性在肿瘤前期的发展过程中增加,可能标志着从感染到炎症驱动的癌变的转变。这些酶可以作为检测胃癌发生的早期生物标志物。我们的研究强调了基于voc的探针通过组织活检实时监测胃癌进展的潜力。因此,本研究证明了诱导挥发组学在研究生物过程和发现新的治疗策略方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Induced volatolomics to uncover new enzymatic hallmarks of precancerous lesions: a proof of concept on gastric preneoplasia in mice
Induced volatolomics is an emerging field of research that offers new opportunities in biology by detecting volatile reporters released by activatable probes, enabling the exploration of oncogenic processes. Building on its proven efficiency in exploring the evolution of implanted tumours, we hypothesized that induced volatolomics could extend its application to detect precancerous conditions. As a proof of concept, we performed a longitudinal study and investigated glycosidase activity during the early stages of gastric carcinogenesis development induced by Helicobacter felis infection in mice, mimicking the gastric carcinogenesis cascade induced by chronic Helicobacter pylori infection in humans. We identified upregulated exoglycosidases linked to acute infections or inflammatory processes in tissues infected by Helicobacter. Specifically, α-mannosidase and β-galactosidase activities in stomach tissue were found to be strongly associated with the initial stages of Helicobacter infection. Additionally, the activities of β-Glucuronidase and β-N-acetyl-glucosaminidase increased during the progression to preneoplastic stages, potentially signalling the transition from infection to inflammation-driven carcinogenesis. These enzymes may serve as early biomarkers for detecting gastric carcinogenesis. Our study highlights the potential of VOC-based probes for real-time monitoring of gastric cancer progression through tissue biopsies. Therefore, this study demonstrates the potential of induced volatolomics for investigating biological processes and uncovering new therapeutic strategies.
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来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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