9-氟-5-烷基-12(H)-基诺[3,4-b][1,4]苯并噻唑氯与DNA相互作用的初步光谱研究

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-05-29 DOI:10.1016/j.bbrep.2025.102067

Aleksandra Owczarzy , Wojciech Rogóż , Karolina Kulig , Andrzej Zięba , Małgorzata Maciążek-Jurczyk

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引用次数: 0

摘要

癌症治疗是现代医学面临的挑战之一。新型抗癌药物的开发需要对其作用机制和构效关系有全面的了解。9-氟-5-烷基-12(H)-基诺[3,4-b][1,4]苯并噻唑氯（Salt2）是一种新合成的具有良好抗癌活性的物质。脱氧核糖核酸（DNA）是一种具有重要生物学意义的大分子。DNA对细胞发育至关重要，包括DNA复制、转录和翻译。小分子与DNA结合可抑制或修饰细胞DNA功能并诱导细胞死亡。反过来，它可以减轻或控制疾病。研究的目的是利用紫外可见光谱、荧光光谱和圆二色光谱来评估一种新开发的物质（Salt2）与小牛胸腺DNA （ctDNA）的相互作用。紫外-可见和荧光分析表明，在基态下，Salt2可能与ctDNA形成强的插层络合物。由于Salt2能够改变ctDNA的结构，因此可以推测，Salt2可以影响DNA的复制、转录和翻译过程，抑制或修饰细胞DNA的功能。Salt2-ctDNA复合物的结果不仅鼓励了进一步的研究，而且可能在确定与人类细胞DNA的结合方面证明是有用的。

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Preliminary spectroscopic studies of the interactions between 9-fluoro-5-alkyl-12(H)-quino[3,4-b][1,4]benzothiazine chloride and DNA

Cancer treatment is one of the challenges of modern medicine. The advancement of new anticancer drugs requires a comprehensive understanding of their mechanism of action and structure-activity relationships. 9-fluoro-5-alkyl-12(H)-quino[3,4-b][1,4]benzothiazine chloride (Salt2) is a newly synthesized substance exhibiting promising anticancer activity. Deoxyribonucleic acid (DNA) is a macromolecule of high biological importance. DNA is essential for cellular development, including DNA replication, transcription and translation. Binding of small molecules to DNA can inhibit or modify cellular DNA function and induces cell death. In turn, it can allow to alleviate or control the disease.

The objective of the research was to assess the interactions of a newly developed substance (Salt2) and calf thymus DNA (ctDNA) using UV-VIS, spectrofluorescence and circular dichroism spectroscopy. The UV-VIS and fluorescence analysis showed that Salt2 might form a strong, intercalative complex in the ground state with ctDNA.

Due to the fact that Salt2 changes ctDNA structure it can be assumed, that Salt2 can effect on DNA replication, transcription and translation processes and inhibits or modifies cellular DNA function. The results obtained for the Salt2-ctDNA complex, not only encourage further research, but may also prove useful in determining binding to human cellular DNA.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.