Biochemistry and Biophysics Reports最新文献

{"title":"Assessment of waterlogging-induced changes in enzymatic antioxidants and carbohydrate metabolism in peanuts genotypes","authors":"Shubhangani Sharma ,&nbsp;Upma Bhatt ,&nbsp;Garishma shah ,&nbsp;Vineet Soni","doi":"10.1016/j.bbrep.2024.101794","DOIUrl":"10.1016/j.bbrep.2024.101794","url":null,"abstract":"<div><p>Soil flooding, manifesting as submergence or waterlogging stress, significantly impacts plant species composition and agricultural productivity, particularly in regions with low rainfall. This study investigates the biochemical responses of two peanut (<em>Arachis hypogaea</em> L.) genotypes, DH-86 and GJG-32, under waterlogging stress. The experiment involved in-vivo pot trials where peanut plants were subjected to continuous waterlogging for 12 days at the flowering stage. Biochemical analyses of leaves conducted and revealed significant alterations in enzyme activities and metabolite concentrations. Key findings include variations in superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPOD), α-amylase, invertase, acid phosphomonoesterase activities, and changes in starch, proline, reducing sugars, and chlorophyll content. SOD, CAT, and GPOD activities exhibited differential responses between genotypes, highlighting DH-86's quicker recovery post-waterlogging. Notably, DH-86 demonstrated higher resilience, reflected in its rapid normalization of biochemical parameters, while GJG-32 showed prolonged stress effects. These findings underscore the importance of antioxidative enzyme systems in mitigating oxidative damage induced by waterlogging. This study enhances our understanding of the biochemical adaptations of peanut genotypes to waterlogging stress, offering valuable insights for breeding programs focused on improving flood tolerance in crops.</p></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"39 ","pages":"Article 101794"},"PeriodicalIF":2.3,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405580824001584/pdfft?md5=081045748aa36cd4ac0fe13c8b4d071b&pid=1-s2.0-S2405580824001584-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141960909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SUMO1 modification of 0N4R-tau is regulated by PIASx, SENP1, SENP2, and TRIM11","authors":"Harmony Wada,&nbsp;Takuma Maruyama ,&nbsp;Takako Niikura","doi":"10.1016/j.bbrep.2024.101800","DOIUrl":"10.1016/j.bbrep.2024.101800","url":null,"abstract":"<div><p>Tau is a microtubule-associated protein that contributes to cytoskeletal stabilization. Aggregation of tau proteins is associated with neurodegenerative disorders such as Alzheimer's disease. Several types of posttranslational modifications that alter the physical properties of tau proteins have been identified. SUMOylation is a reversible modification of lysine residues by a small ubiquitin-like modifier (SUMO). In this study, we examined the enzymes that regulate the SUMOylation and deSUMOylation of tau in an alternatively spliced form, 0N4R-tau. Among SUMO E3 ligases, we found protein inhibitor of activated STAT (PIAS)xα and PIASxβ increase the levels of SUMOylated tau. The deSUMOylation enzymes sentrin-specific protease (SENP)1 and SENP2 reduced the levels of SUMO-conjugated tau. SUMO1 modification increased the level of phosphorylated tau, which was suppressed in the presence of SENP1. Furthermore, we examined the effect of tripartite motif (TRIM)11, which was recently identified as an E3 ligase for SUMO2 modification of tau. We found that TRIM11 increased the modification of both 2N4R- and 0N4R-tau by SUMO1, which was attenuated by mutation of the target lysine residue to arginine. These findings suggest that the expression and activity of SUMOylation regulatory proteins modulate the physical properties of tau proteins and may contribute to the onset and/or progression of tau-associated neurodegenerative disorders.</p></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"39 ","pages":"Article 101800"},"PeriodicalIF":2.3,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S240558082400164X/pdfft?md5=07e46581f602cff841c8aac5475edc56&pid=1-s2.0-S240558082400164X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141953326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the enigmatic PilY1 of Acidithiobacillus thiooxidans: An in silico analysis","authors":"Araceli Hernández-Sánchez ,&nbsp;Edgar D. Páez-Pérez ,&nbsp;Elvia Alfaro-Saldaña,&nbsp;J. Viridiana García-Meza","doi":"10.1016/j.bbrep.2024.101797","DOIUrl":"10.1016/j.bbrep.2024.101797","url":null,"abstract":"<div><p>Thirty years since the first report on the PilY1 protein in bacteria, only the C-terminal domain has been crystallized; there is no study in which the N-terminal domain, let alone the complete protein, has been crystallized. In our laboratory, we are interested in characterizing the Type IV Pili (T4P) of <em>Acidithiobacillus thiooxidans</em>. We performed an <em>in silico</em> characterization of PilY1 and other pilins of the T4P of this acidophilic bacterium. <em>In silico</em> characterization is crucial for understanding how proteins adapt and function under extreme conditions. By analyzing the primary and secondary structures of proteins through computational methods, researchers can gain valuable insights into protein stability, key structural features, and unique amino acid compositions that contribute to resilience in harsh environments. Here, it is presented a description of the particularities of <em>At. thiooxidans</em> PilY1 through predictor software and homology data. Our results suggest that PilY1 from <em>At. thiooxidans</em> may have the same role as has been described for other PilY1 associated with T4P in neutrophilic bacteria; also, its C-terminal interacts (interface interaction) with the minor pilins PilX, PilW and PilV. The N-terminal region comprises domains such as the vWA and the MIDAS, involved in signaling, ligand-binding, and protein-protein interaction. In fact, the vWA domain has intrinsically disordered regions that enable it to maintain its structure over a wide pH range, not only at extreme acidity to which <em>At. thiooxidans</em> is adapted. The results obtained helped us design the correct methodology for its heterologous expression. This allowed us partially experimentally characterize it by obtaining the N-terminal domain recombinantly and evaluating its acid stability through fluorescence spectroscopy. The data suggest that it remains stable across pH changes. This work thus provides guidance for the characterization of extracellular proteins from extremophilic organisms.</p></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"39 ","pages":"Article 101797"},"PeriodicalIF":2.3,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405580824001614/pdfft?md5=fe5cbd11ca33f8710714c06d667f1e72&pid=1-s2.0-S2405580824001614-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141953099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane-anchored intracellular insulin receptor or insulin-like growth factor-1 receptor elicits ligand-independent downstream signaling","authors":"Akiko Sotozono ,&nbsp;Kazuhiko Namekata ,&nbsp;Xiaoli Guo ,&nbsp;Youichi Shinozaki ,&nbsp;Chikako Harada ,&nbsp;Takahiko Noro ,&nbsp;Tadashi Nakano ,&nbsp;Takayuki Harada","doi":"10.1016/j.bbrep.2024.101799","DOIUrl":"10.1016/j.bbrep.2024.101799","url":null,"abstract":"<div><p>Neurodegenerative diseases including glaucoma affect insulin signaling, and insulin treatment has been shown to reverse the neurodegenerative loss of dendritic complexity in retinal ganglion cells. Therefore, strategies for enhancing or maintaining insulin signaling are worth pursuing to establish new therapies for these diseases. In the present study, we generated constitutively active insulin receptor (F-iIR) and insulin-like growth factor-1 receptor (F-iIGF1R) using a system that forces membrane localization of the intracellular domains of these receptors by farnesylation. Immunohistochemistry and Western blot analysis revealed that F-iIR and F-iIGF1R caused the activation of ERK and AKT in the absence of ligands <em>in vitro</em>. Our results suggest that <em>in vivo</em> effects of F-iIR and F-iIGF1R on the progression of neurodegenerative diseases should be investigated in the future.</p></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"39 ","pages":"Article 101799"},"PeriodicalIF":2.3,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405580824001638/pdfft?md5=1d88575d36b244486e508d2e071d8d0e&pid=1-s2.0-S2405580824001638-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141953098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unconventional localization of PAI-1 in PML bodies: A possible link with cellular growth of endothelial cells","authors":"Pragya Gehlot ,&nbsp;Daniela Brünnert ,&nbsp;Vibha Kaushik ,&nbsp;Arpana Yadav ,&nbsp;Saloni Bage ,&nbsp;Kritika Gaur ,&nbsp;Mahesh Saini ,&nbsp;Jens Ehrhardt ,&nbsp;Gowrang Kasaba Manjunath ,&nbsp;Abhishek Kumar ,&nbsp;Neena Kasliwal ,&nbsp;Ajay Kumar Sharma ,&nbsp;Marek Zygmunt ,&nbsp;Pankaj Goyal","doi":"10.1016/j.bbrep.2024.101793","DOIUrl":"10.1016/j.bbrep.2024.101793","url":null,"abstract":"<div><p>Plasminogen activator inhibitor-1 (PAI-1/Serpin E1) is classically known for its antifibrinolytic activity via inhibiting uPA and tPA of the fibrinolytic pathway. PAI-1 has a paradoxical role in tumor progression, and its molecular functions are poorly understood. PAI-1 is a widely accepted secretory protease inhibitor, however, a study suggested the localization of PAI-1 in the cytoplasm and the nucleus. Besides the plethora of its biological functions as a secretory protein, intracellular localization, and functions of PAI-1 remain unexplored at the molecular level. In this study, using various <em>in silico</em> approaches, we showed that PAI-1 possesses a nuclear export signal. Using the CRM1-specific inhibitor leptomycin B, we demonstrated that PAI-1 has a functional CRM1-dependent NES, indicating the possibility of its nuclear localization. Further, we confirm that PAI-1 is localized in the nucleus of endothelial cells using fluorescence microscopy and immunoprecipitation. Notably, we identified an unconventional distribution of PAI-1 in the PML bodies of the nucleus of normal endothelial cells, while the protein was restricted in the cytoplasm of slow-growing cells. The data showed that the localization of PAI-1 in PML bodies is highly correlated with the growth potential of endothelial cells. This conditional nucleocytoplasmic shuttling of PAI-1 during the aging of cells could impart a strong link to its age-related functions and tumor progression. Together, this study identifies the novel behavior of PAI-1 that might be linked with cell aging and may be able to unveil the elusive role of PAI-1 in tumor progression.</p></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"39 ","pages":"Article 101793"},"PeriodicalIF":2.3,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405580824001572/pdfft?md5=b929507b1906f3ae6f9d430293b2109a&pid=1-s2.0-S2405580824001572-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141953325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell transcriptomic analysis of immune cell dynamics in the healthy human endometrium","authors":"Kaixing Chen ,&nbsp;Qiaoni Yu ,&nbsp;Qing Sha ,&nbsp;Junyu Wang ,&nbsp;Jingwen Fang ,&nbsp;Xin Li ,&nbsp;Xiaokun Shen ,&nbsp;Binqing Fu ,&nbsp;Chuang Guo","doi":"10.1016/j.bbrep.2024.101802","DOIUrl":"10.1016/j.bbrep.2024.101802","url":null,"abstract":"<div><p>The microenvironment of the endometrial immune system is crucial to the success of placental implantation and healthy pregnancy. However, the functionalities of immune cells across various stages of the reproductive cycle have yet to be fully comprehended. To address this, we conducted advanced bioinformatic analysis on 230,049 high-quality single-cell transcriptomes from healthy endometrial samples obtained during the proliferative, secretory, early pregnancy, and late pregnancy stages. Our investigation has unveiled that proliferative natural killer (NK) cells, a potential source of endometrial NK cells, exhibit the most robust proliferative and differentiation potential during non-pregnant stages. We have also identified similar differentiation trajectories of NK cells originating from proliferative NK cells across four stages. Notably, during early pregnancy, NK cells demonstrate the highest oxidative phosphorylation metabolism activity, and, in conjunction with macrophages and T cells, exhibit the strongest type II interferon response. With spatial transcriptome data, we have discerned that the most robust immune-non-immune interactions are associated with the promotion and inhibition of cell proliferation, differentiation and migration during four stages. Furthermore, we have compiled lists of stage-specific risk genes implicated in reproductive diseases, which hold promise as potential disease biomarkers. Our study provides insights into the dynamics of the endometrial immune microenvironment during different reproductive cycle stages, thus serving as a reference for detecting pathological changes during pregnancy.</p></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"39 ","pages":"Article 101802"},"PeriodicalIF":2.3,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405580824001663/pdfft?md5=6ad485d812ad13d77d73173bf99cebf2&pid=1-s2.0-S2405580824001663-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141953097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing cellular insights: Super-resolution STORM imaging of cytoskeletal structures in human stem and cancer cells","authors":"Anupam Bharadwaj ,&nbsp;Amalesh Kumar ,&nbsp;Sam Padalumavunkal Mathew ,&nbsp;Rumela Mitra ,&nbsp;Jina Bhattacharyya ,&nbsp;Bithiah Grace Jaganathan ,&nbsp;Bosanta R. Boruah","doi":"10.1016/j.bbrep.2024.101798","DOIUrl":"10.1016/j.bbrep.2024.101798","url":null,"abstract":"<div><p>Fluorescence microscopy is an important tool for cell biology and cancer research. Present-day approach of implementing advanced optical microscopy methods combined with immunofluorescence labelling of specific proteins in cells is now able to deliver optical super-resolution up to ∼25 nm. Here we perform super-resolved imaging using standard immunostaining protocol combined with easy stochastic optical reconstruction microscopy (easySTORM) to observe structural differences of two cytoskeleton elements, actin and tubulin in three different cell types namely human bone marrow-derived mesenchymal stem cells (MSCs), human glioblastoma (U87MG) and breast cancer (MDAMB-231) cells. The average width of the actin bundle obtained from STORM images of stem cells is observed to be larger than the same for U87MG and MDAMB-231 cells. No significant difference is however noticed in the width of the tubulin within the same cells. We also study the functional effect on the 2D migration potential of MDAMB-231 cells silenced for NICD1 and β-catenin. Although similar migration speed is observed for cells with the above two conditions compared to their control cells, easySTORM images show that widths of the actin in MDAMB-231 cells in β-catenin silenced is significantly lower than the same in control cells. Such minute differences however are not observable in widefield images. The outcome of our easySTORM investigation should benefit the researchers carrying out detailed investigations of the cellular structure and potential therapeutic applications.</p></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"39 ","pages":"Article 101798"},"PeriodicalIF":2.3,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405580824001626/pdfft?md5=87ea0d7421c8cff10c28655193391ba5&pid=1-s2.0-S2405580824001626-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141953324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NanoLuciferase technology-based detection of TMPRSS2 as attempt to develop anti-coronavirus agents","authors":"Yanwen Chen ,&nbsp;Yunqi Li ,&nbsp;Ye Zhao ,&nbsp;Lei Pei ,&nbsp;Ling Zhang ,&nbsp;Duowu Zou","doi":"10.1016/j.bbrep.2024.101783","DOIUrl":"10.1016/j.bbrep.2024.101783","url":null,"abstract":"","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"39 ","pages":"Article 101783"},"PeriodicalIF":2.3,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S240558082400147X/pdfft?md5=a2be8a7f9f049a6c72d2051b1eed1e04&pid=1-s2.0-S240558082400147X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141950729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changing the gravity vector direction by inverted culture enhances radiation-induced cell damage","authors":"Yuma Mizoguchi ,&nbsp;Masao Kamimura ,&nbsp;Kazuki Kitabatake ,&nbsp;Fumiaki Uchiumi ,&nbsp;Shin Aoki ,&nbsp;Mitsutoshi Tsukimoto","doi":"10.1016/j.bbrep.2024.101792","DOIUrl":"10.1016/j.bbrep.2024.101792","url":null,"abstract":"<div><p>In recent years, it has become clear that the cytotoxicity of γ-irradiation of cells is increased under microgravity conditions. However, there has been no study of the effect of the gravity vector direction, rather than the magnitude, on γ-ray-induced cytotoxicity. Therefore, in this study, we inverted cultures of human bronchial epithelium BEAS-2B cells and human lung cancer A549 cells in order to change the gravity vector direction by 180° with respect to the cells and observed the cellular response to radiation in this state. We found that cells in inverted culture showed increased irradiation-induced production of reactive oxygen species and decreased expression of the antioxidant protein thioredoxin-1 compared to cells in normal culture. Furthermore, the DNA damage response was delayed in γ-irradiated cells in inverted culture, and the number of unrepaired DNA sites was increased, compared to irradiated cells in normal culture. γ-Ray-induced cell death and the number of G₂-M arrested cells were increased in inverted culture, in accordance with the decreased capacity for DNA repair. Our findings suggest that the gravity vector direction, as well as its magnitude, alters the cellular response to radiation.</p></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"39 ","pages":"Article 101792"},"PeriodicalIF":2.3,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405580824001560/pdfft?md5=8e62de7d5a06cc2d0d77db78ac06eb2c&pid=1-s2.0-S2405580824001560-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141960591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of the substrate preference of the sialidases, CpNanI, HpNanH, and BbSia2 towards 2-Aminobenzamide-labeled 3′-Sialyllactose, 6′-Sialyllactose, and Sialyllacto-N-tetraose-b","authors":"Madhu Lata ,&nbsp;T.N.C. Ramya","doi":"10.1016/j.bbrep.2024.101791","DOIUrl":"10.1016/j.bbrep.2024.101791","url":null,"abstract":"<div><p>Sialidases catalyze the removal of terminal sialic acids from sialylated biomolecules, and their substrate preference is frequently indicated in terms of the glycosidic linkages cleaved (α2-3, α2-6, and α2-8) without mention of the remaining sub-terminal reducing-end saccharide moieties. Many human gut commensal and pathogenic bacteria secrete sialidases to forage for sialic acids, which are then utilized as an energy source or assimilated into membrane/capsular structural components. Infant gut commensals similarly utilize sialylated human milk oligosaccharides containing different glycosidic linkages. Here, we have studied the preference of the bacterial sialidases, BbSia2 from <em>Bifidobacterium bifidum</em>, CpNanI from <em>Clostridium perfringens</em>, and HpNanH from <em>Glaesserella parasuis,</em> for the glycosidic linkages, Siaα2-3Gal, Siaα2-6Gal, and Siaα2-6GlcNAc, by employing 2-Aminobenzamide-labeled human milk oligosaccharides, 3′-Sialyllactose (3′-SL), 6′-Sialyllactose (6′-SL), and Sialyllacto-N-tetraose-b (LSTb), respectively, as proxies for these glycosidic linkages. BbSia2, <em>Cp</em>NanI, and HpNanH hydrolyzed these three oligosaccharides with the glycosidic linkage preferences, 3<strong>′</strong>-SL (Siaα2-3Gal) ≥ LSTb (Siaα2-6GlcNAc) ≥ 6<strong>′</strong>-SL (Siaα2-6Gal), 3<strong>′</strong>-SL (Siaα2-3Gal) ≥ 6<strong>′</strong>-SL (Siaα2-6Gal) &gt; LSTb (Siaα2-6GlcNAc), and 3′-SL (Siaα2-3Gal) ≥ 6′-SL (Siaα2-6Gal) &gt; LSTb (Siaα2-6GlcNAc), respectively. Our finding suggests that sub-terminal reducing-end saccharide moieties can profoundly influence the substrate preference of sialidases, and advocates for the characterization and indication of the substrate preference of sialidases in terms of both the glycosidic linkage and the sub-terminal reducing-end saccharide moiety.</p></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"39 ","pages":"Article 101791"},"PeriodicalIF":2.3,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405580824001559/pdfft?md5=a8867ffa7d9ca5cdc50a234153f56605&pid=1-s2.0-S2405580824001559-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141728916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}