Biochemistry and Biophysics Reports最新文献_第8页

Endogenous Plant signals and human Health: Molecular mechanisms, ecological functions, and therapeutic Prospects 内源性植物信号与人类健康：分子机制、生态功能和治疗前景

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-27 DOI: 10.1016/j.bbrep.2025.102114

Esther Ugo Alum , David Chukwu Obasi , Jacinta Nnennaya Abba , Ugonna Cassandra Aniokete , Prince Nkemakolam Okoroh , Okechukwu Paul-Chima Ugwu , Daniel Ejim Uti

{"title":"Endogenous Plant signals and human Health: Molecular mechanisms, ecological functions, and therapeutic Prospects","authors":"Esther Ugo Alum , David Chukwu Obasi , Jacinta Nnennaya Abba , Ugonna Cassandra Aniokete , Prince Nkemakolam Okoroh , Okechukwu Paul-Chima Ugwu , Daniel Ejim Uti","doi":"10.1016/j.bbrep.2025.102114","DOIUrl":"10.1016/j.bbrep.2025.102114","url":null,"abstract":"<div><div>Endogenous plant signals, including phytohormones, secondary metabolites, and volatile organic compounds, play pivotal roles in plant growth, defense, and ecological interactions. Signals are crucial in plant responses to both biotic and abiotic stressors, as well as in the biosynthesis of therapeutic compounds. Jasmonic acid, salicylic acid, and ethylene are crucial signaling molecules that regulate internal and external communication, including herbivore defense and microbial interactions. Volatile organic compounds further enable plant–plant communication and ecological resilience. Increasing evidence links these signaling pathways to the production of compounds with antioxidant, anti-inflammatory, and anticancer properties in humans, bridging plant ecology with human health. This narrative review was conducted through integrative thematic synthesis of peer-reviewed literature published between 2015 and 2025, using databases such as PubMed, Scopus, and ScienceDirect. Articles were selected based on their relevance to the molecular mechanisms, ecological roles, and therapeutic implications of endogenous plant signals. Emphasis was placed on interdisciplinary studies spanning molecular biology, pharmacology, and systems ecology. This review explores recent advancements in plant signals' molecular and ecological roles, emphasizing their importance in sustainable agriculture, drug discovery, and functional foods. Signaling networks' complexity necessitates interdisciplinary strategies involving molecular biology, systems ecology, and pharmacology, which can be harnessed through biotechnology and systems-based research for therapeutic and ecological innovations.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102114"},"PeriodicalIF":2.3,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neoadjuvant PD-1 blockade induces the autophagy of immune cells: a new target for synergistic therapy of recurrent glioblastoma 新辅助PD-1阻断诱导免疫细胞自噬：复发性胶质母细胞瘤协同治疗的新靶点

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-27 DOI: 10.1016/j.bbrep.2025.102119

Zixue Xuan , Kai Wang , Qingxia Zhu , Ting Sun , Jinying Jiang , Zhongxiu Wu , Shuilian Zheng , Hongying Zhao

{"title":"Neoadjuvant PD-1 blockade induces the autophagy of immune cells: a new target for synergistic therapy of recurrent glioblastoma","authors":"Zixue Xuan , Kai Wang , Qingxia Zhu , Ting Sun , Jinying Jiang , Zhongxiu Wu , Shuilian Zheng , Hongying Zhao","doi":"10.1016/j.bbrep.2025.102119","DOIUrl":"10.1016/j.bbrep.2025.102119","url":null,"abstract":"<div><h3>Background</h3><div>Neoadjuvant PD-1 blockade may incidentally modulate autophagy in immune cells, which could contribute to drug resistance and tumor relapse. However, the specific immune cell subsets affected by neoadjuvant PD-1 blockade in terms of autophagy remain to be fully elucidated, as well as the drugs that might influence these processes.</div></div><div><h3>Methods</h3><div>Single-cell sequencing data from tissues of recurrent glioblastoma (GBM.rec) and GBM treated with neoadjuvant PD-1 blockade (GBM.PD1) were analyzed to investigate the changes in autophagy within immune cells in the GBM.PD1 group. Subsequently, the functional characteristics of subtypes regulated by membrane proteins were explored, and potential drugs targeting key immune cell subsets mediated by these proteins were identified.</div></div><div><h3>Results</h3><div>Neoadjuvant PD-1 blockade significantly increased the proportion of lymphoid cells with elevated autophagy. This elevated autophagy level was associated with specific ligand-receptor interactions in GBM, such as HLA-DRA–CD4. Immune cell subtypes, particularly those with both lymphoid and myeloid signatures (L + M cells, APOE + cells), exhibited strong associations with autophagy. These L + M cells demonstrated significantly more T cell-related interactions in the GBM.PD1 group, with notable receptor-ligand interactions like GZMA-F2R. Furthermore, ribavirin, which targets CXCL8 and IL6, was identified as a potential drug candidate for targeting L + M cells.</div></div><div><h3>Conclusion</h3><div>L + M cells may represent critical immune components involved in autophagy induced by neoadjuvant PD-1 blockade. The interactions between HLA-DRA and CD4, as well as between GZMA and F2R, are crucial for modulating immune responses. Moreover, ribavirin, targeting CXCL8 and IL6, has the potential to enhance the efficacy of neoadjuvant PD-1 blockade.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102119"},"PeriodicalIF":2.3,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesendodermal cells fail to contribute to heart formation following blastocyst injection 胚泡注射后，中胚层细胞不能促进心脏的形成

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-27 DOI: 10.1016/j.bbrep.2025.102120

Biyi Li, Chulan Kwon

{"title":"Mesendodermal cells fail to contribute to heart formation following blastocyst injection","authors":"Biyi Li, Chulan Kwon","doi":"10.1016/j.bbrep.2025.102120","DOIUrl":"10.1016/j.bbrep.2025.102120","url":null,"abstract":"<div><div>Blastocyst complementation offers an opportunity for generating transplantable whole organs from donor sources. Pluripotent stem cells (PSCs) have traditionally served as the primary donor cells due to their ability to differentiate into any type of body cell. However, the use of PSCs raises ethical concerns, particularly regarding their uncontrollable differentiation potential to undesired cell lineages such as brain and germline cells. To address this issue, various strategies have been explored, including the use of genetically modified PSCs with restricted lineage potential or lineage-specified progenitor cells as donors. In this study, we tested whether nascent mesendodermal cells (MECs), which appear during early gastrulation, can be used as donor cells. To do this, we induced Bry-GFP<sup>+</sup> MECs from mouse embryonic stem cells (ESCs) and introduced them into the blastocyst. While donor ESCs gave rise to various regions of embryos, including the heart, Bry-GFP<sup>+</sup> MECs failed to contribute to the host embryos. This finding suggests that MECs, despite being specified from PSCs within a few days, lack the capacity to assimilate into the developing embryo.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102120"},"PeriodicalIF":2.3,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of phosphodiesterase inhibitors on platelet function 磷酸二酯酶抑制剂对血小板功能的影响

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-26 DOI: 10.1016/j.bbrep.2025.102115

Ravi Hochuli, Valerie Dicenta, Zoi Laspa, Manuel Sigle, Tobias Harm, Tatsiana Castor, Anne-Katrin Rohlfing, Meinrad Paul Gawaz

{"title":"Effect of phosphodiesterase inhibitors on platelet function","authors":"Ravi Hochuli, Valerie Dicenta, Zoi Laspa, Manuel Sigle, Tobias Harm, Tatsiana Castor, Anne-Katrin Rohlfing, Meinrad Paul Gawaz","doi":"10.1016/j.bbrep.2025.102115","DOIUrl":"10.1016/j.bbrep.2025.102115","url":null,"abstract":"<div><div>Phosphodiesterase enzymes (PDEs) play a pivotal role in regulating platelet activity by modulating intracellular levels of cAMP and cGMP. Modulation of PDE-2, -3 and -5 activity by suitable inhibitors has been found to reduce platelet activity, and thus thrombus formation.</div><div>Our aim was to study Ibudilast effects on platelet activation, degranulation and aggregation. Therefore, we used the nonspecific PDE inhibitors IBMX as well as the PDE-5 inhibitor Sildenafil as controls. Platelet agonists collagen-related peptide (CRP-A), adenosine diphosphate (ADP) and thrombin receptor activator peptide (TRAP6) were used to induce distinct activation pathways. PDE inhibition was quantified by western blot analysis. Platelet activity was assessed using flow cytometry, light transmission aggregometry and in vitro thrombus formation.</div><div>Inhibition of all platelet PDEs by IBMX substantially reduced platelet activation and aggregation in response to all tested platelet agonists. Ibudilast preferentially inhibits PDE-3 in platelets. Ibudilast decreased platelet activation and aggregation induced by ADP and TRAP, but not CRP-A. Sildenafil alone induced no reduction in PDE activity, platelet activation or aggregation. However, the combination of Sildenafil and Ibudilast had an additive effect on platelet activation. Interestingly, all tested PDE inhibitors demonstrated a significant effect on platelet-dependent thrombus formation.</div><div>In conclusion, the effect of PDE inhibitors on platelet function is influenced by two primary factors: the pharmacological target of the inhibitor and the cAMP/cGMP interaction with the activation pathways induced. Platelet activation by ADP via P<sub>2</sub>Y<sub>12</sub> and TRAP via PAR1 showed a greater response to PDE inhibitors than platelet activation by CRP via GPVI.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102115"},"PeriodicalIF":2.3,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of Fc glycosylation on IgG susceptibility to hinge region chemical reduction: implications for the development of immunoassays Fc糖基化对IgG对铰链区化学还原的敏感性的影响：对免疫测定发展的影响

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-25 DOI: 10.1016/j.bbrep.2025.102112

Vanessa Susini, Silvia Ursino, Chiara Sanguinetti, Alice Botti, Laura Caponi, Maria Franzini

{"title":"The impact of Fc glycosylation on IgG susceptibility to hinge region chemical reduction: implications for the development of immunoassays","authors":"Vanessa Susini, Silvia Ursino, Chiara Sanguinetti, Alice Botti, Laura Caponi, Maria Franzini","doi":"10.1016/j.bbrep.2025.102112","DOIUrl":"10.1016/j.bbrep.2025.102112","url":null,"abstract":"<div><div>Antibodies are glycoproteins, and Fc glycosylation plays a critical structural role in maintaining the proper folding of the C<sub>H</sub>2 domain. Deglycosylated IgGs exhibit an open C<sub>H</sub>2 domain conformation that structurally affects also the neighboring hinge region. Selective reduction of disulfide bonds in this region using mild reducing agents generates monovalent thiol-containing IgGs (rIgGs), which can be immobilized on modified surfaces to orient the Fab fragment outward, enhancing antigen binding efficiency and assay sensitivity. This study investigates the effect of Fc glycosylation on IgG chemical reduction and its implications for in-house ELISA and commercial immunoassays. IgGs were enzymatically deglycosylated with Endo S and then reduced to rIgGs by 2-mercaptoethylamine. Deglycosylation and reduction efficiency were verified by non-reducing SDS-PAGE. Glycosylated and deglycosylated rIgGs were tested in in-house ELISA and commercial immunoassays. Results showed that deglycosylation significantly improves rIgG production, probably by increasing hinge-region accessibility to reducing agents. This led to a 20-fold increase in ELISA sensitivity compared to glycosylated rIgGs. Deglycosylation also mitigates batch-to-batch variability in IgG reduction, enabling consistent rIgG yields. These findings highlight the capability of deglycosylation to standardize rIgG production, broadening its applications in diagnostic immunoassays and biosensing technologies.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102112"},"PeriodicalIF":2.3,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144470849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive method of isolating proteins from serum, cerebrospinal fluid, and hippocampal neurons in rats for proteomic profiling using the LC-MS/MS platform 利用LC-MS/MS平台从大鼠血清、脑脊液和海马神经元中分离蛋白质进行蛋白质组学分析的综合方法

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-25 DOI: 10.1016/j.bbrep.2025.102113

Pratibha Sharma, Rajinder K. Dhamija

{"title":"A comprehensive method of isolating proteins from serum, cerebrospinal fluid, and hippocampal neurons in rats for proteomic profiling using the LC-MS/MS platform","authors":"Pratibha Sharma, Rajinder K. Dhamija","doi":"10.1016/j.bbrep.2025.102113","DOIUrl":"10.1016/j.bbrep.2025.102113","url":null,"abstract":"<div><div>Neurology research largely utilizes rat brains due to their structural and functional similarities to humans, making them valuable models for studying various neurological conditions. There is growing interest in investigating diseases such as Alzheimer's disease (AD), Parkinson's disease (PD), cognition, and other mental health-related disorders. This has created a need for a comprehensive, combined, and easy-to-follow method to isolate serum, cerebrospinal fluid (CSF), and hippocampal neurons. The hippocampus, responsible for learning and memory, is affected by various neurological and psychiatric disorders. However, obtaining samples like CSF and hippocampal neurons is challenging, especially from small animals like rats. Currently, there is no efficient method for isolating these samples altogether from a single animal, and its use in downstream applications has not been thoroughly tested. We have developed a comprehensive and streamlined method for isolating serum, CSF, and hippocampal neurons from a single animal, suitable for downstream applications such as proteomics and biomarker research. This method involves using high-speed centrifugation instruments and density gradient centrifugation, which are easy to follow. The isolated proteins were identified through mass spectrometry. Our method has been successfully tested for high-throughput applications with small sample volumes, demonstrating its clinical utility. With our simplified approach, proteins in serum, CSF, and neural cells can be studied simultaneously. The method achieves ease of use, cost-effectiveness, and reproducibility, thereby facilitating a better understanding of neurological disorders.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102113"},"PeriodicalIF":2.3,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144470850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caloric restriction mimetics chlorogenic acid and fisetin as potential autophagy inducers targeting ATG101 热量限制模拟绿原酸和非西汀作为靶向ATG101的潜在自噬诱导剂

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-24 DOI: 10.1016/j.bbrep.2025.102081

Apoorv Sharma , Indu Kumari , Asimul Islam , Hridayesh Prakash , Amresh Prakash , Vijay Kumar

{"title":"Caloric restriction mimetics chlorogenic acid and fisetin as potential autophagy inducers targeting ATG101","authors":"Apoorv Sharma , Indu Kumari , Asimul Islam , Hridayesh Prakash , Amresh Prakash , Vijay Kumar","doi":"10.1016/j.bbrep.2025.102081","DOIUrl":"10.1016/j.bbrep.2025.102081","url":null,"abstract":"<div><div>Autophagy is an important cytoprotective process impaired in neurodegenerative diseases such as Alzheimer's disease. The initiation process is mediated by the protein kinase Unc-51-like kinase 1 (ULK1) complex. ATG101, a cytosolic protein, plays a pivotal role in initiating autophagy as a component of the ULK complex in mammalian cells. It is important to understand the regulatory processes of individual autophagy components under different conditions for the development of therapeutic interventions. The caloric restriction mimetics (CRMs) such as chlorogenic acid (CGA) and fisetin mimic the healthy outcomes of caloric restriction without decreasing caloric consumption, constituting promising therapeutic candidates for neuroprotection. We explored the ATG101 interactions of CGA and fisetin in this work. Molecular docking and molecular dynamics (MD) simulations were used to investigate the interactions of these CRMs with ATG101, evaluating binding stability and dynamics. To confirm these interactions, we conducted quantitative real-time PCR (qRT-PCR) in differentiated SHSY5Y cells, analyzing the effect of CGA and fisetin on ATG101 gene expression. Our results indicated that fisetin forms a more stable complex with ATG101 compared to CGA. Yet, at the transcriptional level, both CRMs stimulate the mRNA level of ATG101. Therefore, these CRMs can be responsible for their potential as autophagy inducers. These findings offer significant insights into the molecular processes through which CRMs may improve neurodegenerative diseases by triggering autophagy.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102081"},"PeriodicalIF":2.3,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melphalan improves toxicity of arsenic trioxide in adult T-cell leukemia/lymphoma cells 美法兰改善三氧化二砷对成人t细胞白血病/淋巴瘤细胞的毒性

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-24 DOI: 10.1016/j.bbrep.2025.102109

Faeze Khodadadi , Mohammad Hadi Sadeghian , Zahra Delbari , Mohadese Kazemi , Hamide Koohpaykar , Fatemeh B. Rassouli

{"title":"Melphalan improves toxicity of arsenic trioxide in adult T-cell leukemia/lymphoma cells","authors":"Faeze Khodadadi , Mohammad Hadi Sadeghian , Zahra Delbari , Mohadese Kazemi , Hamide Koohpaykar , Fatemeh B. Rassouli","doi":"10.1016/j.bbrep.2025.102109","DOIUrl":"10.1016/j.bbrep.2025.102109","url":null,"abstract":"<div><div>Adult T-cell leukemia/lymphoma (ATLL) is a hematologic neoplasm with poor prognosis. Melphalan is an alkylating anti-cancer agent, and arsenic trioxide (ATO) is routine chemotherapy drug for ATLL with low response rate. Due to the significant challenge that chemoresistance poses in treating ATLL, we aimed to investigate the potential of melphalan to enhance the effects of ATO as a combinatorial treatment approach for ATLL.</div><div>MT-2 cells were exposed to different concentrations of melphalan and ATO and viability was evaluated by alamarBlue assay. Upon IC<sub>50</sub> determination, cells were treated with 0.5 μg/ml melphalan and 2 μM ATO for 72 h, and changes induced on the cell cycle were analyzed by PI staining and flow cytometry, while the expression of candidate genes was assessed by quantitative PCR. For <em>in silico</em> analysis, the expression of <em>ABCG2</em> was assessed in MT-2 cells and ATLL subtypes using GEO database, and molecular docking was performed to predict the interaction of melphalan with this drug transporter.</div><div>Melphalan enhanced the cytotoxicity of ATO up to 32.05 %, and caused accumulation of cells in the sub G<sub>1</sub> phase of the cell cycle. Besides, combination of melphalan and ATO induced considerable reduction in <em>c-MYC, BMI-1</em>, <em>CD44</em> and <em>NF-κB</em> (<em>REL-A</em>) expression. Volcano plots revealed the overexpression of <em>ABCG2</em> in MT-2 cells and acute and smoldering ATLL subtypes, and molecular docking indicated favorable affinity of melphalan with ABCG2. Current findings provide valuable insights into the mechanism of action of melphalan and highlight the importance of targeting drug transporters in improving chemotherapy efficacy in ATLL.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102109"},"PeriodicalIF":2.3,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural product-based inhibitors of quorum sensing: A novel approach to combat antibiotic resistance 基于天然产物的群体感应抑制剂：对抗抗生素耐药性的新方法

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-24 DOI: 10.1016/j.bbrep.2025.102111

Esther Ugo Alum , Bashar Haruna Gulumbe , Sylvester Chibueze Izah , Daniel Ejim Uti , Patrick Maduabuchi Aja , Ikechuku Okorie Igwenyi , Christian Emeka Offor

{"title":"Natural product-based inhibitors of quorum sensing: A novel approach to combat antibiotic resistance","authors":"Esther Ugo Alum , Bashar Haruna Gulumbe , Sylvester Chibueze Izah , Daniel Ejim Uti , Patrick Maduabuchi Aja , Ikechuku Okorie Igwenyi , Christian Emeka Offor","doi":"10.1016/j.bbrep.2025.102111","DOIUrl":"10.1016/j.bbrep.2025.102111","url":null,"abstract":"<div><div>The global rise of antibiotic resistance (AR) presents a critical threat to public health, prompting the search for innovative antimicrobial strategies. Quorum sensing (QS), a bacterial communication system that governs virulence, biofilm formation, and resistance, has emerged as a compelling non-lethal therapeutic target. This review explores the potential of natural product-based quorum sensing inhibitors (QSIs) derived from plants, microbes, and marine organisms. These compounds disrupt QS pathways through various mechanisms, including inhibition of signal synthesis, receptor antagonism, enzymatic degradation of signaling molecules, and suppression of QS-regulated gene expression. Particular emphasis is placed on the molecular targets and synergistic potential of natural QSIs when combined with conventional antibiotics to enhance efficacy and curb resistance development. This narrative review explores the diverse sources of natural QSIs, including plant-derived phytochemicals, microbial secondary metabolites, and marine bioactive compounds. In this study, a thorough literature search was conducted using databases such as PubMed, Scopus, Web of Science, and Google Scholar. Studies selected were those published between 2013 and 2025 in peer-reviewed journals. The manuscript also examines translational challenges such as poor bioavailability, bacterial adaptability, and regulatory barriers, alongside innovative strategies including nanoparticle-based delivery and combination therapies. Finally, clinical and industrial applications of QSIs are discussed, reinforcing their promise as sustainable tools for combating resistant infections and biofilm-associated diseases. This review underscores QS inhibition as a transformative approach in the ongoing battle against antibiotic resistance.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102111"},"PeriodicalIF":2.3,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The duplexity of insulin: The integrated bioinformatics analysis and machine learning identified key genes for type 2 diabetes 胰岛素的多重性：综合生物信息学分析和机器学习确定了2型糖尿病的关键基因

IF 2.3

Biochemistry and Biophysics Reports Pub Date : 2025-06-24 DOI: 10.1016/j.bbrep.2025.102099

Nan Gao , Xiteng Chen , Jun Yang , Yuanfeng Jiang , Shaochong Bu , Xiaomei Bai , Zhenyu Kou , Chunjun Li , Fang Tian

{"title":"The duplexity of insulin: The integrated bioinformatics analysis and machine learning identified key genes for type 2 diabetes","authors":"Nan Gao , Xiteng Chen , Jun Yang , Yuanfeng Jiang , Shaochong Bu , Xiaomei Bai , Zhenyu Kou , Chunjun Li , Fang Tian","doi":"10.1016/j.bbrep.2025.102099","DOIUrl":"10.1016/j.bbrep.2025.102099","url":null,"abstract":"<div><h3>Background</h3><div>Insulin therapy is still the most important treatment for T2DM, but the discussion about whether insulin brings more benefits or harms to T2DM patients has not stopped. Therefore, we used high-throughput RNA sequencing to investigate the role of insulin in T2DM and its molecular changes.</div></div><div><h3>Method</h3><div>We collected peripheral blood samples from 16 patients with T2DM, and performed RNA-seq on peripheral blood mononuclear cells. Bioinformatics analysis and machine learning were uesd to identify the key differential genes and transcription factor networks. In addition, we performed the flow cytometry and staining to observe ROS level and endothelial-monocyte adhesion in PBMCs of both groups.</div></div><div><h3>Results</h3><div>A total of 529 differential genes were identified by bioinformatics analysis. 8 genes were identified as key genes, among which IL-6 had high importance in the random forest model. In transcription factor analysis, IL-6, RETN, CTSG and ELANE have abundant transcriptional regulatory relationships. Flow cytometry showed that ROS production, phagocytosis, leukocyte adhesion in insulin treatment group were lower than that in non-insulin treatment group.</div></div><div><h3>Conclusion</h3><div>Insulin therapy is bidirectional, it can cause islet B cell damage and vascular complications, but also can reduce the level of inflammation and oxidative stress.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"43 ","pages":"Article 102099"},"PeriodicalIF":2.3,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0