黑曲霉纤维素酶抑菌、抗肿瘤和抗sars -cov-2性能的体外评价

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-06-13 DOI:10.1016/j.bbrep.2025.102080

Mahmoud S. Abdelnabi , Nouran H. Assar , Amal S. Shahat , Omnia Kutkat , Rehab Bahy

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引用次数: 0

摘要

微生物酶，特别是纤维素酶，广泛应用于工业生产过程；然而，它们的治疗潜力仍未被充分发掘。本研究研究了黑曲霉以椰子废料为底物生产的一种纤维素酶的抗菌、抗肿瘤和抗病毒特性。方法采用固态发酵法生产纤维素酶。测定了对金黄色葡萄球菌（ATCC 29737）、铜绿假单胞菌（ATCC 25619）和MRSA的抑菌活性。考察了纤维素酶与阿奇霉素的协同抗膜作用。采用MTT法、DPPH自由基清除活性、caspase-3活化和细胞因子谱（TNF-α、IL-6、IL-10）分析对MDA-MB-231乳腺癌细胞的细胞毒性。还检测了对SARS-CoV-2的抗病毒活性。结果所得纤维素酶活性为3.7 U/mL。没有观察到直接的杀菌效果，但与阿奇霉素显著协同减少生物膜生物量。该酶降低癌细胞活力，增加抗氧化（DPPH）活性，升高caspase-3水平，并通过降低TNF-α和IL-6而增加IL-10来调节细胞因子的表达。值得注意的是，该酶制剂对SARS-CoV-2表现出很强的抗病毒活性。结论黑曲霉纤维素酶具有抗生物膜、抗癌、免疫调节和抗病毒等生物学活性。这些发现表明微生物纤维素酶在治疗开发中的应用前景广阔。

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In vitro evaluation of antimicrobial, antitumor, and anti-SARS-cov-2 properties of cellulase enzyme produced by Aspergillus niger

Background

Microbial enzymes, particularly cellulases, are widely used in industrial processes; however, their therapeutic potential remains underexplored. This study investigates the antibacterial, antitumor, and antiviral properties of a cellulase enzyme produced by Aspergillus niger using coir waste as a substrate.

Methods

Cellulase production was carried out via solid-state fermentation. Antimicrobial activity was evaluated against Staphylococcus aureus (ATCC 29737), Pseudomonas aeruginosa (ATCC 25619), and MRSA. The synergistic antibiofilm effect of cellulase with azithromycin was assessed. Cytotoxicity against MDA-MB-231 breast cancer cells was analyzed using MTT assay, DPPH radical scavenging activity, caspase-3 activation, and cytokine profiling (TNF-α, IL-6, IL-10). Antiviral activity against SARS-CoV-2 was also examined.

Results

The produced cellulase exhibited an activity of 3.7 U/mL. No direct bactericidal effect was observed, but a significant synergistic reduction in biofilm biomass was noted with azithromycin. The enzyme reduced cancer cell viability, increased antioxidant (DPPH) activity, elevated caspase-3 levels, and modulated cytokine expression by decreasing TNF-α and IL-6 while increasing IL-10. Notably, the enzyme formulation demonstrated strong antiviral activity against SARS-CoV-2.

Conclusion

Cellulase derived from A. niger presents significant biological potential, including antibiofilm, anticancer, immunomodulatory, and antiviral properties. These findings suggest promising applications of microbial cellulase in therapeutic development.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.