Bioactive Materials最新文献_第7页

Advanced biomaterials for targeting mature biofilms in periodontitis therapy

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-27 DOI: 10.1016/j.bioactmat.2025.02.026

Jiawen Tao , Yirong Sun , Guoliang Wang , Jingru Sun , Shujun Dong , Jianxun Ding

{"title":"Advanced biomaterials for targeting mature biofilms in periodontitis therapy","authors":"Jiawen Tao , Yirong Sun , Guoliang Wang , Jingru Sun , Shujun Dong , Jianxun Ding","doi":"10.1016/j.bioactmat.2025.02.026","DOIUrl":"10.1016/j.bioactmat.2025.02.026","url":null,"abstract":"<div><div>Periodontitis is a chronic inflammatory disease primarily caused by bacteria, leading to inflamed and bleeding gums, periodontal pocket formation, and bone loss. Affecting 70%–90% of adults over 65, periodontitis is a leading cause of tooth loss and significantly impacts quality of life. Standard treatments, including subgingival scraping and antibiotics, have limitations, and antibiotic resistance among periodontal pathogens is an increasing concern. Biofilms are barriers to drugs and immune responses, contributing to bacterial resistance and reducing antibiotic effectiveness. Due to their adjustable physicochemical properties, bioactive materials potentially eliminate bacterial biofilms, presenting a promising alternative for periodontitis therapy. In this review, the recent innovations in biomaterials for removing mature biofilms in periodontitis are examined, and their broader potential is discussed. Additionally, the compositions of bacterial biofilms, formation pathways, and intrinsic drug resistance mechanisms are discussed. Finally, the strategies for optimizing subgingival biofilm removal in periodontitis are highlighted, such as targeting biofilms-embedded bacteria, disrupting the extracellular polymeric substances, and utilizing combined approaches. A comprehensive understanding of the properties of biomaterials guides the rational design of highly targeted and effective therapies for periodontitis.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 474-492"},"PeriodicalIF":18.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metastructure and strain-defect engineered Cu-doped TiOx coating to enhance antibacterial sonodynamic therapy

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-27 DOI: 10.1016/j.bioactmat.2025.02.028

Songsong Wang , Ji Tan , Haifeng Zhang , Shiwei Guan , Yibo Zeng , Xiaoshuang Nie , Hongqin Zhu , Shi Qian , Xuanyong Liu

{"title":"Metastructure and strain-defect engineered Cu-doped TiOx coating to enhance antibacterial sonodynamic therapy","authors":"Songsong Wang , Ji Tan , Haifeng Zhang , Shiwei Guan , Yibo Zeng , Xiaoshuang Nie , Hongqin Zhu , Shi Qian , Xuanyong Liu","doi":"10.1016/j.bioactmat.2025.02.028","DOIUrl":"10.1016/j.bioactmat.2025.02.028","url":null,"abstract":"<div><div>Sonodynamic therapy (SDT) has attracted widespread attention in treatment of implant-associated infections, one of the key factors leading to implant failure. Nevertheless, constructing efficient ultrasound-triggered coatings on implant surfaces remains a challenge. Herein, an acoustic metastructure Cu-doped defective titanium oxide coating (Cu-TiO<sub><em>x</em></sub>) with lattice strain was constructed <em>in situ</em> on titanium implant to realize effective sonocatalysis. The redistribution of Cu atoms broke the pristine lattice of TiO<sub>2</sub> during the thermal reduction treatment to regulate its energy structure, which favored separation of electron-hole pairs generated by ultrasound radiation to enhance the sonocatalytic generation of reactive oxygen species. In addition, the acoustic metastructure enhanced the absorption of ultrasound by Cu-TiO<sub><em>x</em></sub> metastructure coating, which further promoted its sonocatalytic effect. Thus, Cu-TiO<sub><em>x</em></sub> metastructure coating could efficiently eliminate <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> infections under ultrasonic irradiation in 10 min. Besides, the osteogenic property of implant was significantly improved after infection clearance <em>in vivo</em>. This work provides a fresh perspective on the design of SDT biosurfaces based on metastructure and strain-defect engineering.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 458-473"},"PeriodicalIF":18.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A micro-metabolic rewiring assay for assessing hypoxia-associated cancer metabolic heterogeneity

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-27 DOI: 10.1016/j.bioactmat.2025.02.030

Jeong Min Oh , Tianze Guo , Hydari Masuma Begum , Saci-Elodie Marty , Liang Sha , Cem Kilic , Hao Zhou , Yali Dou , Keyue Shen

{"title":"A micro-metabolic rewiring assay for assessing hypoxia-associated cancer metabolic heterogeneity","authors":"Jeong Min Oh , Tianze Guo , Hydari Masuma Begum , Saci-Elodie Marty , Liang Sha , Cem Kilic , Hao Zhou , Yali Dou , Keyue Shen","doi":"10.1016/j.bioactmat.2025.02.030","DOIUrl":"10.1016/j.bioactmat.2025.02.030","url":null,"abstract":"<div><div>Cancer metabolism plays an essential role in therapeutic resistance, where significant inter- and intra-tumoral heterogeneity exists. Hypoxia is a prominent driver of metabolic rewiring behaviors and drug responses. Recapitulating the hypoxic landscape in the tumor microenvironment thus offers unique insights into heterogeneity in metabolic rewiring and therapeutic responses, to inform better treatment strategies. There remains a lack of scalable tools that can readily interface with imaging platforms and resolve the heterogeneous behaviors in hypoxia-associated metabolic rewiring. Here we present a micro-metabolic rewiring (μMeRe) assay that provides the scalability and resolution needed to characterize the metabolic rewiring behaviors of different cancer cells in the context of hypoxic solid tumors. Our assay generates hypoxia through cellular metabolism without external gas controls, enabling the characterization of cell-specific intrinsic ability to drive hypoxia and undergo metabolic rewiring. We further developed quantitative metrics that measure the metabolic plasticity through phenotypes and gene expression. As a proof-of-concept, we evaluated the efficacy of a metabolism-targeting strategy in mitigating hypoxia- and metabolic rewiring-induced chemotherapeutic resistance. Our study and the scalable platform thus lay the foundation for designing more effective cancer treatments tailored toward specific metabolic rewiring behaviors.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 493-509"},"PeriodicalIF":18.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microenvironment-responsive coating for vascular stents to regulate coagulation-inflammation interaction and promote vascular recovery

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-26 DOI: 10.1016/j.bioactmat.2025.02.031

Qiongjun Zhu , Zhezhe Chen , Dan'an Wang , Xiaolu Jiao , Yi Luan , Min Wang , Rifang Luo , Yunbing Wang , Guosheng Fu , Yanan Wang , Wenbin Zhang

{"title":"Microenvironment-responsive coating for vascular stents to regulate coagulation-inflammation interaction and promote vascular recovery","authors":"Qiongjun Zhu , Zhezhe Chen , Dan'an Wang , Xiaolu Jiao , Yi Luan , Min Wang , Rifang Luo , Yunbing Wang , Guosheng Fu , Yanan Wang , Wenbin Zhang","doi":"10.1016/j.bioactmat.2025.02.031","DOIUrl":"10.1016/j.bioactmat.2025.02.031","url":null,"abstract":"<div><div>Early coagulation-inflammation interaction and late in-stent restenosis undermine the efficacy of vascular stents after implantation. Targeting the interplay between inflammation and coagulation, and smooth muscle cell (SMC) proliferation, we presented a microenvironment-responsive coating designed to regulate tissue responses and vascular regeneration throughout the remodeling process. Coagulation was inhibited by incorporating anticoagulant tirofiban into the coating. MMP9-responsive nanoparticles embedded in the coating released salvianolic acid A to modulate inflammatory cell behavior and inhibit SMC dysfunction. By effectively interfering with clotting and inflammation, the coating suppressed platelet-fibrin interaction and formation of platelet-monocyte aggregates, thereby mitigating adverse effects on reendothelialization. Its ability to influence SMC proliferation and migration resulted in reduced intimal hyperplasia. Coated stents were shown to significantly regulate tissue regeneration, improve the vascular environment and even reduced the lipid content in the narrowed atherosclerotic vessels <em>in viv</em>o. This direct approach enhanced the vascular tissue regeneration after stent implantation, and offered promising insights for optimizing vascular stent design.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 443-457"},"PeriodicalIF":18.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Living joint prosthesis with in-situ tissue engineering for real-time and long-term osteoarticular reconstruction

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-26 DOI: 10.1016/j.bioactmat.2025.01.036

Wei Sun , Hongwei Wu , Yiyang Yan , Xianzhu Zhang , Xudong Yao , Rui Li , Jingyi Zuo , Wenyue Li , Hongwei Ouyang

{"title":"Living joint prosthesis with in-situ tissue engineering for real-time and long-term osteoarticular reconstruction","authors":"Wei Sun , Hongwei Wu , Yiyang Yan , Xianzhu Zhang , Xudong Yao , Rui Li , Jingyi Zuo , Wenyue Li , Hongwei Ouyang","doi":"10.1016/j.bioactmat.2025.01.036","DOIUrl":"10.1016/j.bioactmat.2025.01.036","url":null,"abstract":"<div><div>The reconstruction of large osteoarticular defects caused by tumor resection or severe trauma remains a clinical challenge. Current metal prostheses exhibit a lack of osteo-chondrogenic functionality and demonstrate poor integration with host tissues. This often results in complications such as abnormal bone absorption and prosthetic loosening, which may necessitate secondary revisions. Here, we propose a paradigm-shifting “living prosthesis” strategy that combines a customized 3D-printed hollow titanium humeral prosthesis with engineered bone marrow condensations presenting bone morphogenetic protein-2 (BMP-2) and transforming growth factor–β3 (TGF-β3) from encapsulated silk fibroin hydrogels. This innovative approach promotes <em>in situ</em> endochondral defect regeneration of the entire humeral head while simultaneously providing immediate mechanical support. In a rabbit model of total humerus resection, the designed “living prosthesis” achieved weight, macroscopic and microscopic morphologies that were comparable to those of undamaged native joints at 2 months post-implantation, with organized osteochondral tissues were regenerated both around and within the prosthesis. Notably, the “living prosthesis” displayed significantly higher osteo-integration than the blank metal prosthesis did, as evidenced by a 3-fold increase in bone ingrowth and a 2-fold increase in mechanical pull-out strength. Furthermore, the \"living prosthesis\" restored joint cartilage function, with rabbits exhibiting normal gait and weight-bearing capacity. The successful regeneration of fully functional humeral head tissue from a single implanted prosthesis represents technical advance in designing bioactive bone prosthesis, with promising implications for treating extreme-large osteochondral defects.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 431-442"},"PeriodicalIF":18.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ UNIversal Orthogonal Network (UNION) bioink deposition for direct delivery of corneal stromal stem cells to corneal wounds

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-24 DOI: 10.1016/j.bioactmat.2025.02.009

Lucia G. Brunel , Betty Cai , Sarah M. Hull , Uiyoung Han , Thitima Wungcharoen , Gabriella Maria Fernandes-Cunha , Youngyoon Amy Seo , Patrik K. Johansson , Sarah C. Heilshorn , David Myung

{"title":"In situ UNIversal Orthogonal Network (UNION) bioink deposition for direct delivery of corneal stromal stem cells to corneal wounds","authors":"Lucia G. Brunel , Betty Cai , Sarah M. Hull , Uiyoung Han , Thitima Wungcharoen , Gabriella Maria Fernandes-Cunha , Youngyoon Amy Seo , Patrik K. Johansson , Sarah C. Heilshorn , David Myung","doi":"10.1016/j.bioactmat.2025.02.009","DOIUrl":"10.1016/j.bioactmat.2025.02.009","url":null,"abstract":"<div><div>The scarcity of human donor corneal graft tissue worldwide available for corneal transplantation necessitates the development of alternative therapeutic strategies for treating patients with corneal blindness. Corneal stromal stem cells (CSSCs) have the potential to address this global shortage by allowing a single donor cornea to treat multiple patients. To directly deliver CSSCs to corneal defects within an engineered biomatrix, we developed a UNIversal Orthogonal Network (UNION) collagen bioink that crosslinks <em>in situ</em> with a bioorthogonal, covalent chemistry. This cell-gel therapy is optically transparent, stable against contraction forces exerted by CSSCs, and permissive to the efficient growth of corneal epithelial cells. Furthermore, CSSCs remain viable within the UNION collagen gel precursor solution under standard storage and transportation conditions. This approach promoted corneal transparency and re-epithelialization in a rabbit anterior lamellar keratoplasty model, indicating that the UNION collagen bioink serves effectively as an <em>in situ</em>-forming, suture-free therapy for delivering CSSCs to corneal wounds.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 414-430"},"PeriodicalIF":18.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

4D printing polymeric biomaterials for adaptive tissue regeneration

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-22 DOI: 10.1016/j.bioactmat.2025.01.033

Zhe Wang , Duo Ma , Juan Liu , Shi Xu , Fang Qiu , Liqiu Hu , Yueming Liu , Changneng Ke , Changshun Ruan

引用次数: 0

Precision repair of zone-specific meniscal injuries using a tunable extracellular matrix-based hydrogel system

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-22 DOI: 10.1016/j.bioactmat.2025.02.013

Se-Hwan Lee , Zizhao Li , Ellen Y. Zhang , Dong Hwa Kim , Ziqi Huang , Yuna Heo , Sang Jin Lee , Hyun-Wook Kang , Jason A. Burdick , Robert L. Mauck , Su Chin Heo

{"title":"Precision repair of zone-specific meniscal injuries using a tunable extracellular matrix-based hydrogel system","authors":"Se-Hwan Lee , Zizhao Li , Ellen Y. Zhang , Dong Hwa Kim , Ziqi Huang , Yuna Heo , Sang Jin Lee , Hyun-Wook Kang , Jason A. Burdick , Robert L. Mauck , Su Chin Heo","doi":"10.1016/j.bioactmat.2025.02.013","DOIUrl":"10.1016/j.bioactmat.2025.02.013","url":null,"abstract":"<div><div>Meniscus injuries present significant therapeutic challenges due to their limited self-healing capacity and the diverse biological and mechanical properties across the tissue. Conventional repair strategies do not replicate the complex zonal characteristics within the meniscus, resulting in suboptimal outcomes. In this study, we introduce an innovative fetal/adult and stiffness-tunable meniscus decellularized extracellular matrix (DEM)-based hydrogel system designed for precision repair of heterogeneous, zonal-dependent meniscus injuries. By synthesizing fetal and adult DEM hydrogels, we identified distinct cellular responses, including that hydrogels with adult meniscus-derived DEM promote more fibrochondrogenic phenotypes. The incorporation of methacrylated hyaluronic acid (MeHA) further refined the mechanical properties and injectability of the DEM-based hydrogels. The combination of fetal and adult DEM with MeHA allowed for precise tuning of stiffness, influencing cell differentiation and closely mimicking native tissue environments. <em>In vivo</em> tests confirmed the biocompatibility of hydrogels and their integration with native meniscus tissues. Furthermore, advanced 3D bioprinting techniques enabled the fabrication of hybrid hydrogels with biomaterial and mechanical gradients, effectively emulating the zonal properties of meniscus tissue and enhancing cell integration. This study represents a significant advance in meniscus tissue engineering, providing a promising platform for customized regenerative therapies across a range of heterogeneous fibrous connective tissues.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 400-413"},"PeriodicalIF":18.0,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macrophage-targeting Antisenescence nanomedicine enables in-Situ NO induction for Gaseous and antioxidative atherosclerosis intervention

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-20 DOI: 10.1016/j.bioactmat.2025.02.025

Yuanyuan Peng , Wei Feng , Hui Huang , Yu Chen , Shaoling Yang

{"title":"Macrophage-targeting Antisenescence nanomedicine enables in-Situ NO induction for Gaseous and antioxidative atherosclerosis intervention","authors":"Yuanyuan Peng , Wei Feng , Hui Huang , Yu Chen , Shaoling Yang","doi":"10.1016/j.bioactmat.2025.02.025","DOIUrl":"10.1016/j.bioactmat.2025.02.025","url":null,"abstract":"<div><div>Senescent-endothelial cells significantly accelerate atherosclerosis progression, making the mitigation of cellular aging a promising strategy for treating the disease. Nitric oxide (NO), a low molecular weight and lipophilic gas, has been shown to penetrate cell membranes effectively and delay cell senescence. In this study, we designed and engineered osteopontin (OPN)-modified nanoliposomes (CZALO) that encapsulate L-arginine (L-Arg) and cerium-zirconium oxide nanoparticles (CZ NPs), which exhibit enzyme-like activities for targeted atherosclerosis treatment. Following inflammatory chemotaxis and OPN-mediated internalization by macrophages, CZ NPs released from CZALO nanoliposomes significantly scavenge reactive oxygen species, thereby inhibiting cholesterol uptake and promoting macrophage phenotypic transformation, resulting in both antioxidant and anti-inflammatory effects. Additionally, nitric oxide synthase (NOS) overexpressed in macrophages catalyzes L-Arg to produce NO, which is then selectively released in situ and diffuses into endothelial cells, exerting anti-aging effects by regulating senescence-associated secretory phenotype factor secretion, enhancing lysosomal function, alleviating cell cycle arrest, and reducing DNA damage. The antioxidant and anti-aging effects of CZALO nanoliposomes collectively alleviate atherosclerotic burden with minimal toxicity both <em>in vitro</em> and <em>in vivo</em>. This “two-birds-one-stone” nanotherapeutic offers a novel approach for regulating vascular microenvironment homeostasis and improving therapeutic efficiency in atherosclerosis treatment.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 294-312"},"PeriodicalIF":18.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D-printed PCL scaffolds combined with injectable sodium alginate/magnesium-doped mesoporous bioactive glass nanosphere hydrogel for meniscus regeneration: In vitro, In vivo, and multiomics-based therapeutic analyses

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-20 DOI: 10.1016/j.bioactmat.2025.02.016

Hao Li , Yongkang Yang , Tianze Gao , Runmeng Li , Chao Wang , Xue Wang , Tianyuan Zhao , Qinyu Tian , Zhixing Zhang , Ruiyang Zhang , Quanyi Guo , Zhiguo Yuan , Peifu Tang

{"title":"3D-printed PCL scaffolds combined with injectable sodium alginate/magnesium-doped mesoporous bioactive glass nanosphere hydrogel for meniscus regeneration: In vitro, In vivo, and multiomics-based therapeutic analyses","authors":"Hao Li , Yongkang Yang , Tianze Gao , Runmeng Li , Chao Wang , Xue Wang , Tianyuan Zhao , Qinyu Tian , Zhixing Zhang , Ruiyang Zhang , Quanyi Guo , Zhiguo Yuan , Peifu Tang","doi":"10.1016/j.bioactmat.2025.02.016","DOIUrl":"10.1016/j.bioactmat.2025.02.016","url":null,"abstract":"<div><div>Meniscal injury presents a formidable challenge and often leads to functional impairment and osteoarthritic progression. Meniscus tissue engineering (MTE) is a promising solution, as conventional strategies for modulating local immune responses and generating a conducive microenvironment for effective tissue repair are lacking. Recently, magnesium-containing bioactive glass nanospheres (Mg-BGNs) have shown promise in tissue regeneration. However, few studies have explored the ability of Mg-BGNs to promote meniscal regeneration. First, we verified the anti-inflammatory and fibrochondrogenic abilities of Mg-BGNs in vitro. A comprehensive in vivo evaluation of a rabbit critical-size meniscectomy model revealed that Mg-BGNs have multiple effects on meniscal reconstruction and effectively promote fibrochondrogenesis, collagen deposition, and cartilage protection. Multiomics analysis was subsequently performed to further explore the mechanism by which Mg-BGNs regulate the regenerative microenvironment. Mechanistically, Mg-BGNs first activate the TRPM7 ion channel through the PI3K/AKT signaling pathway to promote the cellular function of synovium-derived mesenchymal stem cells and then activate the PPARγ/NF-κB axis to modulate macrophage polarization and inflammatory reactions. We demonstrated that Mg<sup>2+</sup> is critical for the crosstalk among biomaterials, immune cells, and effector cells in Mg-BGN-mediated tissue regeneration. This study provides a theoretical basis for the application of Mg-BGNs as nanomedicines to achieve in situ tissue regeneration in complex intrajoint pathological microenvironments.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 313-335"},"PeriodicalIF":18.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0