Bioactive Materials最新文献

{"title":"3D printing of chitosan hydrogel reinforced with tubular nanoclay for hemostasis and infected wound healing","authors":"Xiangyu Chen ,&nbsp;Youquan Zhou ,&nbsp;Shuiqing Zhou ,&nbsp;Di Zhang ,&nbsp;Luying Zeng ,&nbsp;Changren Zhou ,&nbsp;Mingxian Liu","doi":"10.1016/j.bioactmat.2025.08.024","DOIUrl":"10.1016/j.bioactmat.2025.08.024","url":null,"abstract":"<div><div>Developing hydrogel dressings capable of rapid hemostasis and accommodating irregular wounds is crucial for infected wound healing. Customizable 3D-printed hydrogels, highly sought after for wound management, remain an ongoing challenge due to weak mechanical strength and low bioactivity. This work develops a nanoclay-enhanced, three-dimensional (3D) printed chitosan (CS) hydrogel for controlling bleeding and bacterial-infected wound healing. The hydrogen bonding and electrostatic attraction between halloysite clay nanotubes (HNTs) and CS endow the CS/HNTs ink with shear-thinning, excellent printability, high fidelity, and shape retention, allowing it to conform to irregular wound shapes. Incorporation of HNTs improved compressive/tensile strength and hemostatic performance of CS hydrogel. Furthermore, the antibacterial agent levofloxacin (Lev) was loaded into CS/HNTs hydrogel, which can be continuously released at the wound site to reduce inflammation and promote healing. In vitro experiments demonstrated that CS/HNTs/Lev hydrogel possesses favorable antibacterial, coagulation, blood compatibility, and cytocompatibility. Additionally, in vivo studies using infected mouse models indicated that the composite hydrogel exhibits rapid hemostatic ability, good antibacterial property, and enhanced wound healing efficacy. The 3D-printed CS hydrogel reinforced with nanoclay shows great potential as a hemostasis and wound healing dressing in clinical application.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"54 ","pages":"Pages 404-422"},"PeriodicalIF":18.0,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autograft-matching wireless bioelectronic conduits: Magneto-electric coupling enabled taurine metabolism activation for peripheral nerve repair","authors":"Anmin Wang ,&nbsp;Wenjing Song ,&nbsp;Wentai Guo ,&nbsp;Baolei Huang ,&nbsp;Xingqiang Zhu ,&nbsp;Hailin Zhang ,&nbsp;Wei Zhai ,&nbsp;Ye Tian ,&nbsp;Li Ren","doi":"10.1016/j.bioactmat.2025.08.023","DOIUrl":"10.1016/j.bioactmat.2025.08.023","url":null,"abstract":"<div><div>Peripheral nerve injuries remain a clinical challenge due to the limitations of autografts and unstable electrical signals in existing bioelectronic therapies. It is necessary to develop innovative strategies to achieve wireless, controllable peripheral neural regeneration (PNR). In this study, we developed a magneto-electric coupling-driven electroactive nerve guidance conduits (PCLG/AgNF NGCs) with a moving magnetic field (MMF) for PNR with wireless magneto-electric coupling electrical stimulation (MECES). PCLG/AgNF displayed high conductivity (25.48 ± 3.77 S/cm) and wireless controllability of generating electrical pulses (16.67 ± 0.47 μA to 475.7 ± 9.71 μA) with an MMF. The MECES produced by PCLG/AgNF with the MMF significantly promoted cell proliferation, cell migration, and upregulated the expression of β3-tubulin, neurofilament heavy chain and growth-associated protein 43, compared to PCLG/AgNF and MMF used individually. Mechanistically, we identified that PCLG/AgNF with the MMF activated the metabolism of taurine and hypotaurine corroborated by elevated intracellular taurine, which is crucial for MECES mediated repair processes. In a rat peripheral nerve defect model, the PCLG/AgNF NGCs with the MMF showed promising results in nerve regrowth, myelination, and functional recovery, performing comparably to autografts. This strategy offers PCLG/AgNF NGCs as a wireless, controllable, precision-enabled approach and provides novel insights for the effective PNR.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"54 ","pages":"Pages 387-403"},"PeriodicalIF":18.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel scaffold encapsulating MSC-Exos and ZIF-8 promotes bone regeneration via coordinating osteogenesis and immunomodulation","authors":"Yunhui Si ,&nbsp;Xiaorong Li ,&nbsp;Shuao Dong ,&nbsp;Xueqin Gao ,&nbsp;Yuetong Zhu ,&nbsp;Linzhen Mo ,&nbsp;Zhiwei Wang ,&nbsp;Shuhan Wang ,&nbsp;Chao Zhang","doi":"10.1016/j.bioactmat.2025.08.026","DOIUrl":"10.1016/j.bioactmat.2025.08.026","url":null,"abstract":"<div><div>The limited bone regeneration and suboptimal immune responses constitute the major challenges in repairing critical-sized bone defects. As an emerging therapeutic modality, mesenchymal stem cell-derived exosomes (MSC-Exos) exhibit promising application prospects in bone regeneration. In this study, the bone-functionalized MSC-Exos are loaded into GelMA hydrogel scaffolds modified with the bone immunomodulator Zeolitic Imidazolate Framework-8 (ZIF-8) for the repair of critical-sized bone defects. The prepared MSC-Exos/ZIF-8@GelMA composite hydrogel demonstrates excellent biocompatibility and favors the adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs). The sustained release of exosomes and zinc ions endows the composite hydrogel with synergistically enhanced bone regeneration, angiogenesis, and immunomodulation. Specifically, the microRNA-23a-3p within internalized MSC-Exos activates the AKT signaling pathway in BMSCs by targeting the PTEN node and up-regulates the expression of osteogenesis-related markers. Meanwhile, it is demonstrated for the first time that ZIF-8 inhibits the activation of the non-classical NF-κB pathway in RAW264.7 cells under simulated inflammatory conditions, thereby downregulating pro-inflammatory cytokine expression and inducing M2-type polarization. The rat cranial bone defect model demonstrates that the composite hydrogel significantly enhances new bone formation and angiogenesis <em>in vivo</em> and maintains a low level of inflammation. The design of a composite hydrogel featuring synergistic enhancement of osteogenesis and immunomodulation represents a novel strategy for developing bone tissue engineering scaffolds.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"54 ","pages":"Pages 329-351"},"PeriodicalIF":18.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Point-of-care treatment of acute skin wound by portable in-situ electrospinning nanofiber dressings with rapid hemostasis, anti-infection, and angiogenesis effects","authors":"Yechuan Deng ,&nbsp;Min Xing ,&nbsp;Yuanming Cao ,&nbsp;Kuicai Ye ,&nbsp;Jiayin Feng ,&nbsp;Shiwei Guan ,&nbsp;Linlin Zhao ,&nbsp;Wenhao Qian ,&nbsp;Jiajun Qiu ,&nbsp;Xuanyong Liu","doi":"10.1016/j.bioactmat.2025.08.020","DOIUrl":"10.1016/j.bioactmat.2025.08.020","url":null,"abstract":"<div><div>The increasing prevalence of acute traumatic injuries caused by traffic accidents, and natural disasters presents multifaceted challenges such as hemorrhages in irregular wounds and being susceptible to microbes. Herein, <em>in situ</em> point-of-care electrospun Zein/Polyvinylpyrrolidone nanofiber bioactive dressings coordinated with tannic acid-based copper nanoparticles (CTZP) are prepared. CTZP exhibits rapid hemostasis performance in rat tail amputation model. And <em>in vitro</em> blood coagulation experiment verifies that CTZP can achieve blood coagulation within 1 min. It has been found that CTZP can activate platelet through the expression of TBXAS1 via PI3K-Akt signaling pathway. Furthermore, the <em>in vitro</em> tube forming assay present that CTZP has an angiogenetic promotion effect. The qPCR result reveals that Zein/Polyvinylpyrrolidone (ZP) substrate can realize angiogenesis promotion by elevating the production of VEGF. Moreover, the addition of tannic acid-based copper (Cu@TA) nanoparticles further enhances VEGF promotion effect and synergistically upregulates the expression of eNOS by PI3K-Akt signaling pathway which is the same as the pathway in platelet activation. In addition, the <em>in vivo</em> immunohistochemistry results confirm the upregulation of VEGF and CD31 which are angiogenesis-related proteins. Besides, Cu@TA nanoparticles endow CTZP dressings with potent antibacterial activity through hydroxyl radical generation via Fenton-like reaction and copper ion release. Eventually, <em>in vivo</em> experiment using an <em>S. aureus</em>-infected rat wound model confirms CTZP's significant wound-healing efficacy. These findings advance the practical application of <em>in situ</em> electrospinning technology for acute trauma care, providing both theoretical and material insights for designing hemostatic, anti-infection, and angiogenetic wound dressings.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"54 ","pages":"Pages 371-386"},"PeriodicalIF":18.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spontaneous dynamic modulation of ionic/immune microenvironment on polyetheretherketone for sequential anti-infection and osseointegration","authors":"Junjie Zhou ,&nbsp;Yinghao Wu ,&nbsp;Ji Tan ,&nbsp;Yisi Liu ,&nbsp;Jibing He ,&nbsp;Bowen Zheng ,&nbsp;Xiaohan Ma ,&nbsp;Xianming Zhang ,&nbsp;Jiaxing Wang ,&nbsp;Shiwei Guan ,&nbsp;Xin Ma ,&nbsp;Xiaochun Peng ,&nbsp;Xuanyong Liu","doi":"10.1016/j.bioactmat.2025.08.001","DOIUrl":"10.1016/j.bioactmat.2025.08.001","url":null,"abstract":"<div><div>Initial implant-related infection and subsequent poor osseointegration are the main causes of implant placement failure. The extracellular microenvironment is an important mediator of behaviors of cell and bacteria; however, spontaneously dynamically regulating the microenvironment to match tissue integration processes remains a challenge. Here, we construct a multilayer film on polyetheretherketone (PEEK) surface with different inner and outer layers of magnesium oxide (MgO) degradation rates. This film can sequentially regulate surface ions and immune microenvironment to achieve sequential antibacteria and bone integration. In the early stage of bone implantation, the outer layer of MgO can rapidly degrade to produce a strong alkaline microenvironment and a large amount of magnesium (Mg) ions, disrupting the energy metabolism of adherent bacteria and inducing M1 polarization of macrophages to enhance their ability to engulf planktonic bacteria. In the later stage, the inner layer MgO can slowly release Mg ion for a long time, synergistically promoting the proliferation and differentiation of osteoblasts by directly stimulating osteoblasts and inducing M2 polarization of macrophages. The rat femoral implantation model confirms the good sequential immune-enhanced antibacteria and bone integration ability of the film <em>in vivo</em>. In addition, the film can control the polarization time of cells by adjusting the thickness of the outer layer to meet the needs of different scenarios. This study demonstrates that the synergistic induction of ion microenvironment and immune microenvironment is a promising and safe surface modification strategy for bone implants.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"54 ","pages":"Pages 311-328"},"PeriodicalIF":18.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sprayable hydrogel sponge for neurovascular microenvironment reconstruction and inflammation modulation in diabetic wound healing","authors":"Xiaozhen Zhou ,&nbsp;Pengchao Ma ,&nbsp;Yihao Liu ,&nbsp;Zhaojian Wang ,&nbsp;Shida Chen ,&nbsp;Zekun Cheng ,&nbsp;Songlu Tseng ,&nbsp;Hui Wu ,&nbsp;Mengdi Zhang ,&nbsp;Fengzhou Du ,&nbsp;Nanze Yu ,&nbsp;Xiao Long ,&nbsp;Jiuzuo Huang ,&nbsp;Xiumei Wang","doi":"10.1016/j.bioactmat.2025.08.008","DOIUrl":"10.1016/j.bioactmat.2025.08.008","url":null,"abstract":"<div><div>Diabetic wounds are characterized by chronic inflammation, vascular insufficiency, and peripheral neuropathy, which collectively disrupt the neurovascular microenvironment essential for coordinated tissue regeneration. However, strategies targeting neurovascular regeneration remain limited. Here, we developed a sprayable hydrogel sponge based on gelatin methacryloyl and methacrylamide-modified ε-poly-L-lysine (S-GPL), co-functionalized with VEGF-mimetic peptide (KLT) and BDNF-mimetic peptide (RGI). The sprayable format conforms to irregular wound geometries, while the pneumatic spraying technique generates high-pressure microbubbles that create a porous structure, thereby enhancing exudate absorption and sustained peptide release as a sponge dressing. Additionally, the incorporation of KLT and RGI facilitates the reconstruction of the neurovascular microenvironment. In vitro, KLT promoted endothelial cell maturation and cytokine secretion, whereas RGI enhanced Schwann cell activity. Notably, S-GPL^KLT/RGI facilitated intercellular interactions between RSCs and HUVECs, highlighting the cellular mechanisms underlying neurovascular communication. In a full-thickness diabetic wound model in rats, the hydrogel accelerated wound closure, re-epithelialization, and matrix remodeling. These effects were accompanied by enhanced neovascularization and axonal regeneration, along with the formation of a spatially organized neurovascular niche, as evidenced by CD31⁺ capillaries closely aligned with PGP9.5⁺ nerve fibers. Building upon the intrinsic anti-inflammatory properties of S-GPL, transcriptomic and immunohistochemical analyses further revealed that S-GPL^KLT/RGI treatment suppressed the IL-17 signaling pathway. However, the relationship between immunomodulation and neurovascular reconstruction warrants further investigation. Collectively, this study presents a sprayable antibacterial hydrogel that not only reconstructs the neurovascular microenvironment but also mitigates chronic inflammation, offering a clinically translatable strategy for diabetic wound management.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"54 ","pages":"Pages 352-370"},"PeriodicalIF":18.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional cell sheet model improves in vitro prediction accuracy of osteogenic potential for biodegradable magnesium-based metals","authors":"Liangwei Chen ,&nbsp;Guanxi Wu ,&nbsp;Siyu Liu ,&nbsp;Ziyu Yan ,&nbsp;Honglei Yue ,&nbsp;Jianhua Zhu ,&nbsp;Na Ge ,&nbsp;Yifei Wang ,&nbsp;Qingxiang Li ,&nbsp;Guanqi Liu ,&nbsp;Tingting Zhang ,&nbsp;Haowen Zheng ,&nbsp;Shaozhe Xin ,&nbsp;Guangyunhao Sun ,&nbsp;Chuanbin Guo ,&nbsp;Jianmin Han","doi":"10.1016/j.bioactmat.2025.08.013","DOIUrl":"10.1016/j.bioactmat.2025.08.013","url":null,"abstract":"<div><div>Biodegradable metals have been increasingly utilized clinically due to their biosafety and pro-osteogenic properties. However, conventional monolayer cell-based preclinical safety evaluation methods based on ISO10993-5 consistently indicate significant cytotoxicity that contradicts <em>in vivo</em> outcomes. In this study, we aimed to establish an <em>in vitro</em> evaluation model that better correlates with <em>in vivo</em> performance. Three-layer BMSC cell sheets were constructed using layer-by-layer assembly. Histological analyses revealed a stable three-dimensional structure with elevated cell-cell interaction proteins, including N-Cadherin, Fibronectin, and Vinculin, along with enhanced osteogenic potential. The cytotoxicity of 4N pure Mg was evaluated in both cell sheet and monolayer co-culture models. Flow cytometry showed higher Ki67 expression and lower ROS levels and apoptosis rate in cell sheets. ShRNA-mediated silencing of N-Cadherin in cell sheets significantly compromised their cytoprotective capacity against Mg metal-induced toxicity. Osteogenesis-related gene expression correlation analysis between <em>in vitro</em> co-culture models and <em>in vivo</em> femur implantation models was conducted using RNA-seq and qRT-PCR. Results showed that 4N pure Mg enhanced osteogenic genes (<em>BMP2R</em>, <em>RUNX2</em>, and <em>SP7</em>) in cell sheets, consistent with <em>in vivo</em> patterns but contrary to monolayer models. Various Mg-based metals (4N/5N Pure Mg, ZE21B, and WE43) were evaluated in cell sheet defect, monolayer defect, and cranial defect models. 5N Pure Mg, ZE21B, and WE43 promoted defect healing in both cranial defect and cell sheets, but showed no positive effect in monolayers. Collectively, cell sheet models correlated well with <em>in vivo</em> results, suggesting their potential as alternative <em>in vitro</em> evaluation models, thereby accelerating clinical translation of Mg-based biomaterials.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"54 ","pages":"Pages 291-310"},"PeriodicalIF":18.0,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jahn-Teller distortion-engineered self-propelled nanorobots for mitochondrial targeting and bioenergetic disruption in tumor therapy","authors":"Tianying Luo ,&nbsp;Mingyi Zhang ,&nbsp;Jia Xu ,&nbsp;Dechao Yuan ,&nbsp;Beibei Yin ,&nbsp;Yuzhou Zhu ,&nbsp;Shuang Yan ,&nbsp;Meng Pan ,&nbsp;Dong Mo ,&nbsp;Xicheng Li ,&nbsp;Xuyue Liang ,&nbsp;Zhaojuan Qin ,&nbsp;Hongxin Deng ,&nbsp;Zhiyong Qian","doi":"10.1016/j.bioactmat.2025.08.021","DOIUrl":"10.1016/j.bioactmat.2025.08.021","url":null,"abstract":"<div><div>Mitochondrial metabolism plays a pivotal role in tumor progression, yet effective therapeutic targeting remains constrained by limited tissue penetration and lack of spatiotemporal control. Herein, we present Jahn-Teller distortion-engineered, self-propelled nanorobots (IDP@Z@AP) that integrate catalytic oxygen generation, mitochondria-targeted drug delivery, and real-time 3D NIR-II photoacoustic (PA) imaging for precision tumor therapy. The nanorobots are fabricated by co-encapsulating a NIR-II photothermal agent (IR1048) and a mitochondria-targeting chemotherapeutic (DOX-TPP) within a ZIF-8 framework, followed by in situ anchoring of ultrasmall AuPt bimetallic nanozymes. Pt-induced Jahn-Teller distortion modulates the electronic structure of AuPt, enhancing glucose oxidase- and catalase-like activities. Under NIR-II laser irradiation, photothermal-enhanced cascade catalysis drives autonomous motion and catalyzes intratumoral O₂ generation, facilitating deep tumor infiltration. <em>In vitro</em> studies reveal efficient mitochondrial targeting, resulting in significant mitochondrial membrane depolarization, intracellular ATP depletion, and suppressed cell migration and invasion. <em>In vivo</em>, 3D NIR-II PA imaging enables noninvasive visualization of nanorobot biodistribution and real-time mapping of catalytic oxygen generation within tumor tissues. This nanorobotic platform effectively modulates tumor hypoxia and enhances chemotherapeutic delivery to mitochondria, ultimately achieving potent tumor suppression. The work offers a smart, catalytically driven, mitochondria-targeted strategy with real-time therapeutic feedback for subcellular-level cancer therapy.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"54 ","pages":"Pages 273-290"},"PeriodicalIF":18.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Front Cover (BAM V52.pdf)","authors":"","doi":"10.1016/S2452-199X(25)00379-2","DOIUrl":"10.1016/S2452-199X(25)00379-2","url":null,"abstract":"","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"52 ","pages":"Page OFC"},"PeriodicalIF":18.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano MgO loaded thermosensitive HPCH@HA hydrogel accelerates in situ bone repair through osteoimmunomodulation while enhancing angiogenesis and osteogenesis","authors":"Feifei Ni ,&nbsp;Longkang Chang ,&nbsp;Yizhong Peng ,&nbsp;Dongxu Li ,&nbsp;Dong Wu ,&nbsp;Kanglu Li ,&nbsp;Xin Zhang ,&nbsp;Xulin Jiang ,&nbsp;Zengwu Shao ,&nbsp;Yangyang Chen ,&nbsp;Hong Wang","doi":"10.1016/j.bioactmat.2025.08.007","DOIUrl":"10.1016/j.bioactmat.2025.08.007","url":null,"abstract":"<div><div>Bone defect repair is a complex physiological process, starting with early modulation by the inflammatory immune system, and involves multiple physiological events, including angiogenesis, osteogenic differentiation, and mineralization. Biomaterial can regulate inflammatory responses through relevant immune cells in the local immune microenvironment of the implant-bone interface which is a hot topic in the field of regenerative medicine. Currently, Mg²⁺ regulates immune cells in the bone microenvironment to promote osteogenesis and angiogenesis mainly focuses on macrophages,but there is relatively little research on T cells.At the same time, the effective delivery and release of Mg²⁺ remains a challenge. To address these issues, we designed a new thermosensitive hyaluronic acid-hydroxypropyl chitin hydrogel (HPCH@HA) that has good affinity for Mg²⁺ and can sustained release it. In vitro, nano MgO loaded complex hydrogels effectively induced macrophage polarization from M0 phenotype to M2 phenotype and simultaneously activate T lymphocytes which also promoted human adipose-derived stem cells (hADSCs) osteogenic differentiation and mineralization and human umbilical vein endothelial cells (HUVECs) angiogenesis. In vivo, at the early stage of repair, the composite hydrogel has a good repair effect on mouse skull critical defect. All these results show that our designed composite hydrogels can effectively regulate the immune microenvironment of bone tissue and promoting the formation of mature bone in large bone defects and supporting in situ bone regeneration without the use of exogenous cells or inducers. It's a promising candidate as immunomodulatory biomaterials for bone tissue engineering purposes.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"54 ","pages":"Pages 248-272"},"PeriodicalIF":18.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}