Bioactive Materials最新文献

{"title":"Engineered nerve guide conduit enhances peripheral nerve regeneration by modulating the microenvironment through macrophage-triggered cascade reactions","authors":"Xindi Yang ,&nbsp;Xiao Liang ,&nbsp;Binyi Wang ,&nbsp;Xing Gao ,&nbsp;Wenqiang Yang ,&nbsp;Jiajun Li ,&nbsp;Huaan Cai ,&nbsp;Zan Tong ,&nbsp;Yun Chen","doi":"10.1016/j.bioactmat.2025.04.033","DOIUrl":"10.1016/j.bioactmat.2025.04.033","url":null,"abstract":"<div><div>Nerve regeneration after peripheral nerve injury (PNI) depends on exogenous and endogenous pro-healing signals in the microenvironment. Maintaining immune system homeostasis and remodeling the regenerative microenvironment are crucial prerequisites for promoting nerve regeneration. Here, a double-layer PSM/Gel-SC(IL-4) nerve guide conduit was fabricated by photocrosslinking poly (ethylene glycol) diacrylate (PEGDA) and methacrylic acid-sodium alginate (SAMA) hydrogel as the outer layer, with GelMA encapsulating IL-4 overexpressing Schwann cells as the inner layer. PSM/Gel-SC(IL-4) conduit enabled the sustained release of IL-4, inducing pro-healing macrophages polarization through JAK1/STAT6 pathway <em>in vitro</em>. The polarized macrophages further triggered the cellular cascade reactions, enhancing the pro-healing characteristics of endothelial cells and Schwann cells. In addition, PSM/Gel-SC(IL-4) conduit significantly promoted functional recovery and nerve regeneration in 12-mm rat sciatic nerve defect model, induced pro-healing macrophage polarization at the early stage and accelerated angiogenesis and myelination at the nerve regeneration stage. This study developed a novel immunomodulatory strategy to remodel the regenerative microenvironment, and explored the underlying cellular and molecular mechanism, demonstrating considerable therapeutic potential for long-distance nerve defect.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"51 ","pages":"Pages 46-69"},"PeriodicalIF":18.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From mechanism to applications: Advanced microneedles for clinical medicine","authors":"Yuqing Yang ,&nbsp;Haifu Sun ,&nbsp;Xiao Sun ,&nbsp;Yu Wang ,&nbsp;Fan Xu ,&nbsp;Wenyu Xia ,&nbsp;Liang Chen ,&nbsp;Manyi Li ,&nbsp;Tianke Yang ,&nbsp;Yusen Qiao ,&nbsp;Dechun Geng","doi":"10.1016/j.bioactmat.2025.04.025","DOIUrl":"10.1016/j.bioactmat.2025.04.025","url":null,"abstract":"<div><div>Advanced microneedles (MNs) are a series of complex and multifunctional MNs designed for a variety of clinical applications that are not only efficacious but also cost-effective, acceptable, and safe. In light of the potential limitations of conventional drug delivery, including low drug bioavailability and insufficient therapeutic efficiency, as well as the financial burden and discomfort associated with conventional detection, there is a thirst for alternatives. As a novel form of cargo delivery capable of penetrating tissue surface barriers easily, MNs avoid the various risks associated with traditional testing tools, and can achieve precise, on-demand, or continuous drug delivery. It has great potential to become an ideal clinical tool. Herein, an exhaustive review is proposed to summarize the designs and research progress of MNs. First, the designs and fabrication of it are introduced. Next, recent advances of applications in detection and therapy of MNs are systematically classified and discussed. Second, clinical trials of MNs in recent five years and commercial MNs are provided. Furthermore, this review discusses the limitations and challenges of MNs and speculates on their prospective evolution and applications in biomedicine. This review focuses on the future effective utilization of MNs and novel designs of innovative and effective clinical MNs devices.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"51 ","pages":"Pages 1-45"},"PeriodicalIF":18.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human induced pluripotent stem cell derived nanovesicles for cardiomyocyte protection and proliferation","authors":"Yuhua Wei ,&nbsp;Xiaoxiao Geng ,&nbsp;Qing You ,&nbsp;Yu Zhang ,&nbsp;Fangfang Cao ,&nbsp;Gunaseelan Narayanan ,&nbsp;Thanh Nguyen ,&nbsp;Xiaoyuan Chen ,&nbsp;Jianyi Zhang ,&nbsp;Lei Ye","doi":"10.1016/j.bioactmat.2025.04.017","DOIUrl":"10.1016/j.bioactmat.2025.04.017","url":null,"abstract":"<div><div>It remains a significant challenge to reactivate the cell cycle activity of adult mammalian cardiomyocytes (CMs). This study created a hypo-immunogenic human induced pluripotent stem cell (hiPSC) line using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing to knockout β2-microglobulin in hiPSCs (^B2MKOhiPSCs) for manufacturing nanovesicles (^B2MKOhiPSC-NVs). Approximately 9500 ^B2MKOhiPSC-NVs were produced from a single ^B2MKOhiPSC. Proteomic analyses indicated that, compared to ^B2MKOhiPSCs, the cargos of ^B2MKOhiPSC-NVs were enriched in spindle and chromosomal proteins, as well as proteins that regulate the cell cycle and scavenge reactive oxygen species (ROS). When administrated to hiPSCs derived CMs (hiPSC-CMs), ^B2MKOhiPSC-NVs reduced lactate dehydrogenase leakage and apoptosis in hypoxia-cultured hiPSC-CMs through activating the AKT pathway, protected hiPSC-CMs from H₂O₂-induced damage by ROS scavengers in the NV cargo, increased hiPSC-CM proliferation via the YAP pathway, and were hypoimmunogenic when co-cultured with human CD8⁺ T cells or delivered to mice. Furthermore, when ^B2MKOhiPSC-NVs or 0.9 % NaCl were intramyocardially injected into mice after cardiac ischemia/reperfusion injury, cardiac function and infarct size, assessed 4 weeks later, were significantly improved in the ^B2MKOhiPSC-NV group, with increased mouse CM survival and cell cycle activity. Thus, the proteins in the ^B2MKOhiPSC-NV cargos convergently activated the AKT pathway, scavenged ROS to protect CMs, and upregulated YAP signaling to induce CM cell cycle activity. Thus, ^B2MKOhiPSC-NVs hold great potential for cardiac protection and regeneration.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 585-602"},"PeriodicalIF":18.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-activated carrier-free nanoprodrugs enhanced universality and efficiency of solid tumor-targeting chemotherapy","authors":"Xiaodan Xu ,&nbsp;Guowei Wang ,&nbsp;Yijie Chen ,&nbsp;Peile Jin ,&nbsp;Jifan Chen ,&nbsp;Xia Fang ,&nbsp;Dequan Ye ,&nbsp;Pintong Huang","doi":"10.1016/j.bioactmat.2025.04.038","DOIUrl":"10.1016/j.bioactmat.2025.04.038","url":null,"abstract":"<div><div>The clinical outcome of chemotherapy for solid tumors is significantly restricted by adverse off-target side effects and heterogeneous microenvironments. Herein, we developed a series of ultrasound (US)-activated carrier-free self-assembled nanoprodrugs (PBSN38-OSs) to enhance universality and efficiency of tumor-targeting chemotherapy. The nanoprodrugs integrated reactive oxygen species (ROS)-responsive pinacol boronic ester-conjugated SN38 (PBSN38) and organic sonosensitizers (OSs). By screening the OSs library, six small molecules with strong binding ability with PBSN38 and high sonodynamic generation efficiency were identified. Then, various PBSN38-OSs nanoprodrugs with high drug-loading content and aqueous stability were fabricated using a facile nano-precipitation method. When exposed to US irradiation, PBSN38-OSs produced extensive ROS <em>in situ</em>, strongly disturbing the endogenous redox balance to overcome the heterogeneity of tumoral ROS content. They subsequently triggered the release of active SN38, thereby resulting in severe oxidative damage and microenvironment-independent cell apoptosis. The antitumor activity and biocompatibility of PBSN38-OSs were thoroughly examined <em>in vitro</em> and <em>in vivo</em>, and two optimal nanoprodrugs were screened, which exhibited potent therapeutical effects toward solid tumor models of colon adenocarcinoma, hepatocellular carcinoma, and pancreatic carcinoma. Overall, the versatile US-activated carrier-free nanoprodrugs could significantly minimize the side effects of chemo-drugs and improve the tumor-targeting chemotherapy efficacy in a spatial-controlled and microenvironment-independent manner, holding great prospects in further clinical translation.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 571-584"},"PeriodicalIF":18.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Angiogenesis in rheumatoid Arthritis: Pathological characterization, pathogenic mechanisms, and nano-targeted therapeutic strategies","authors":"Fang Zhao ,&nbsp;Zeyu Hu ,&nbsp;Gejing Li ,&nbsp;Min Liu ,&nbsp;Qiong Huang ,&nbsp;Kelong Ai ,&nbsp;Xiong Cai","doi":"10.1016/j.bioactmat.2025.04.026","DOIUrl":"10.1016/j.bioactmat.2025.04.026","url":null,"abstract":"<div><div>Angiogenesis is critical from early development through the progression of life-threatening diseases. In rheumatoid arthritis (RA), angiogenesis is markedly heightened and undergoes aberrant changes that exacerbate the progression of synovitis. However, the intricate mechanisms underlying these changes remain poorly understood. Despite the development of numerous anti-angiogenic agents, their clinical efficacy is often compromised by adverse effects and the emergence of adaptive resistance, leading to disease relapse or progression. Nanomedicine has gained significant attention owing to its excellent biocompatibility, precise biological targeting, and enhanced therapeutic outcomes. Anti-angiogenic nanoagents have shown transformative potential in treating cancer and retinal diseases. Nevertheless, a comprehensive review addressing the fundamental mechanisms of anti-angiogenic nanoagents in RA has yet to be undertaken. Herein, this review provides an in-depth description of the unique structural and functional aspects of pathological angiogenesis in RA and its negative consequences. The mechanisms of pro-angiogenic mediators contributing to RA angiogenesis are further explored. Subsequently, biological activities of nanomedicines for the treatment of RA are summarized. Finally, the cutting-edge developments in the anti-angiogenic nanoagents of RA engineered based on these mechanisms and bioactivities are outlined. A helpful introduction to anti-angiogenic strategies for treatment of RA, which may offer novel perspectives for the development of nanoagents, is opening a new horizon in the fight against RA.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 603-639"},"PeriodicalIF":18.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced sequential osteosarcoma therapy using a 3D-Printed bioceramic scaffold combined with 2D nanosheets via NIR-II photothermal-chemodynamic synergy","authors":"Guangyu Jian ,&nbsp;Si Wang ,&nbsp;Xinlu Wang ,&nbsp;Qinyi Lu ,&nbsp;Xingyu Zhu ,&nbsp;Shucheng Wan ,&nbsp;Shan Wang ,&nbsp;Dize Li ,&nbsp;Chao Wang ,&nbsp;Qingqing He ,&nbsp;Tao Chen ,&nbsp;Jinlin Song","doi":"10.1016/j.bioactmat.2025.04.029","DOIUrl":"10.1016/j.bioactmat.2025.04.029","url":null,"abstract":"<div><h3>Background</h3><div>Osteosarcoma (OS) is a malignant tumor originating from primitive mesenchymal cells, characterized by rapid metastasis, high invasiveness, and significant mortality. The primary challenges in OS management include the effective elimination of residual tumor cells to prevent recurrence and the repair of extensive bone defects caused by surgical intervention.</div></div><div><h3>Objective</h3><div>This study aims to develop an innovative biomimetic 3D-printed bioactive glass ceramic (BGC) scaffold modified with two-dimensional nanosheets to address both tumor ablation and bone tissue repair.</div></div><div><h3>Materials and methods</h3><div>The nanosheets were constructed via ellagic acid (EA) and ruthenium (Ru) coordination, leveraging the non-topological adhesion properties of catechol in EA to deposit the nanosheets onto the BGC scaffold (EARu-BGC). The therapeutic effects of EARu-BGC were evaluated <em>in vitro</em> and <em>in vivo</em>.</div></div><div><h3>Results</h3><div>EARu-BGC sequentially responds to the local microenvironment during OS treatment. During the tumor ablation phase, EARu-BGC induced ferroptosis through the synergistic effects of photothermal and chemodynamic therapy, achieving over 90 % tumor cell ablation and significantly inhibiting tumor volume and weight. In the bone tissue repair phase, EARu-BGC exhibited adaptive ROS scavenging and facilitated a pro-healing microenvironment, promoting osteogenic differentiation. The gradual degradation of the BGC scaffold provided essential minerals and space for new bone formation. In vivo experiments demonstrated that EARu-BGC significantly enhanced osteogenesis, increasing the trabecular number to 1.51 ± 0.15/mm and reducing trabecular separation to 1.50 ± 0.04 mm.</div></div><div><h3>Conclusion</h3><div>The EARu-BGC scaffold presents a promising multifunctional platform for OS treatment by effectively balancing antitumor efficacy with bone repair capabilities.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 540-555"},"PeriodicalIF":18.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microenvironment-responsive living hydrogel containing engineered probiotic for treatment of massive bone defects","authors":"Haoyu Fang ,&nbsp;Yanyi Wang ,&nbsp;Li Li ,&nbsp;Xiaotong Qin ,&nbsp;Daoyu Zhu ,&nbsp;Pei Liu ,&nbsp;Qianhao Yang ,&nbsp;Youshui Gao ,&nbsp;Zhongmin Shi ,&nbsp;Xin Ma ,&nbsp;Chao Zhong ,&nbsp;Yixuan Chen","doi":"10.1016/j.bioactmat.2025.04.020","DOIUrl":"10.1016/j.bioactmat.2025.04.020","url":null,"abstract":"<div><div>Self-activating and microenvironment-responsive biomaterials for tissue regeneration would address the escalating need for bone grafting, but remain challenging. The emergence of microbial living therapeutics offers vast potential in regenerative medicine, as genetically engineered probiotics possess efficient stimuli-responsiveness and tunable biological functions. Here, using elevated endogenous nitric oxide (NO) signals as a biological trigger in bone fracture injuries, a Living Responsive Regenerative Medicine (LRRM) strategy for <em>in situ</em> bone defect repair through real-time controlled release of bone morphogenetic protein-2 (BMP2) is proposed. The <em>Escherichia coli</em> Nissle 1917 (EcN) strain, genetically engineered to sense NO signals and correspondingly produce and secrete BMP2, was firstly encapsulated in gelatin methacryloyl (GelMA) microspheres and then embedded in a bulky hyaluronic acid methacryloyl (HAMA) hydrogel to form a living hydrogel device that circumvents immune attack and prevents bacterial leakage. <em>In vivo</em> multiple bone defect models demonstrated the efficacy of the living hydrogel in enhancing the maturation of bone callus, promoting neovascularization, and facilitating full-thickness bone union. Strategic incorporation of engineered probiotics and the bilayer-structured encapsulation system may emerge as an effective and microenvironment-responsive medicine approach for tissue regeneration.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 556-570"},"PeriodicalIF":18.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of vascularized liver microtissues recapitulates angiocrine-mediated hepatocytes maturation and enhances therapeutic efficacy for acute liver failure","authors":"Liuyang Zhu ,&nbsp;Sen Liu ,&nbsp;Zhuangzhuang Yang ,&nbsp;Long Yang ,&nbsp;Yueyue Yang ,&nbsp;Pinsheng Han ,&nbsp;Yu Miao ,&nbsp;Lei Lin ,&nbsp;Lilin Xu ,&nbsp;Yan Li ,&nbsp;Xinyue Li ,&nbsp;Libo Wang ,&nbsp;Tianyu Zhao ,&nbsp;Weiwei Wang ,&nbsp;Zilin Cui ,&nbsp;Ze Wang ,&nbsp;Deling Kong ,&nbsp;Zhongyang Shen ,&nbsp;Yamin Zhang","doi":"10.1016/j.bioactmat.2025.04.030","DOIUrl":"10.1016/j.bioactmat.2025.04.030","url":null,"abstract":"<div><div>Liver failure poses a significant challenge for millions of patients. The use of primary human hepatocytes and the engineering of liver organoids or liver tissue provide promising solutions to mitigate the shortage of donor organs. However, insufficient vascularization and functional immaturity remain major barriers impeding optimal functional recovery after transplantation. In this study, adult stem cells derived from human liver tissues were induced to form liver organoids, which were subsequently co-cultured with vascular organoids generated from human induced pluripotent stem cells in a defined ratio to create vascularized liver microtissues. This approach successfully established a complex vascular network analogous to that found in the liver, effectively recapitulating a more physiologically relevant liver architecture. Mechanistically, this configuration promoted the structural and secretory maturation of liver organoids through paracrine signaling from the vasculature. Following transplantation into the mesentery of mice, the vascularized liver microtissues rapidly established connections with the host vasculature and enhanced secretion of albumin into the bloodstream. Moreover, the transplantation of vascularized liver microtissues could effectively ameliorate liver injury and inflammatory responses, reduce apoptosis while promoting cell proliferation in CCl₄-induced acute liver failure mice. These findings provide a robust platform for investigating the interactions between vessels and liver, and have important implications for liver failure treatment in the field of regenerative medicine.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 525-539"},"PeriodicalIF":18.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing advanced computational approaches to design novel antimicrobial peptides against intracellular bacterial infections","authors":"Yanpeng Fang ,&nbsp;Duoyang Fan ,&nbsp;Bin Feng ,&nbsp;Yingli Zhu ,&nbsp;Ruyan Xie ,&nbsp;Xiaorong Tan ,&nbsp;Qianhui Liu ,&nbsp;Jie Dong ,&nbsp;Wenbin Zeng","doi":"10.1016/j.bioactmat.2025.04.016","DOIUrl":"10.1016/j.bioactmat.2025.04.016","url":null,"abstract":"<div><div>Intracellular bacterial infections pose a significant challenge to current therapeutic strategies due to the limited penetration of antibiotics through host cell membranes. This study presents a novel computational framework for efficiently screening candidate peptides against these infections. The proposed strategy comprehensively evaluates the essential properties for the clinical application of candidate peptides, including antimicrobial activity, permeation efficiency, and biocompatibility, while also taking into account the speed and reliability of the screening process. A combination of multiple AI-based activity prediction models allows for a thorough assessment of sequences in the cell-penetrating peptides (CPPs) database and quickly identifies candidate peptides with target properties. On this basis, the CPP microscopic dynamics research system was constructed. Exploration of the mechanism of action at the atomic level provides strong support for the discovery of promising candidate peptides. Promising candidates are subsequently validated through <em>in vitro</em> and <em>in vivo</em> experiments. Finally, Crot-1 was rapidly identified from the CPPsite 2.0 database. <strong>Crot-1</strong> effectively eradicated intracellular MRSA, demonstrating significantly greater efficacy than vancomycin. Moreover, it exhibited no apparent cytotoxicity to host cells, highlighting its potential for clinical application. This work offers a promising new avenue for developing novel antimicrobial materials to combat intracellular bacterial infections.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 510-524"},"PeriodicalIF":18.0,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservable, scalable and ready-to-use cell-laden patches for diabetic ulcer treatment","authors":"Bangrui Yu ,&nbsp;Lanlan Peng ,&nbsp;Wenjun Dang ,&nbsp;Ying Fu ,&nbsp;Zhijie Li ,&nbsp;Jinteng Feng ,&nbsp;Heng Zhao ,&nbsp;Tian Wang ,&nbsp;Feng Xu ,&nbsp;Martin L. Yarmush ,&nbsp;Haishui Huang","doi":"10.1016/j.bioactmat.2025.04.024","DOIUrl":"10.1016/j.bioactmat.2025.04.024","url":null,"abstract":"<div><div>Stem cell-laden hydrogel patches are promising candidates to treat chronic ulcers due to cells’ long-lasting and dynamic responses to wound microenvironment. However, their clinical translations are prohibited by the cryopreservation difficulty due to their weak mechanical strength and slow biotransport capability, and by the morphological mismatch between clinical ulcers and pre-fabricated patches. Here we report a stem cell-laden alginate-dopamine hydrogel patch that can be readily cryopreserved, processed, and scaled toward clinical usages. This cell-hydrogel patch not only maintains cell viability and structure integrity during cryopreservation, but also can be directly utilized without centrifugation or incubation post cryopreservation. In addition, this tissue-adhesive hydrogel patch enables close wound contact and fast cellular response, and its scalable and flexible structure enables assembly for large or irregularly shaped ulcers. Therefore, it accelerates ulcer healing and reduces scar formation <em>via</em> continuous, versatile, self-adjusting paracrine of imbedded, cryopreserved stem cells. These findings highlight its potential for scalable clinical applications in chronic wound management and pave the way for broader adoption of ready-to-use regenerative therapies.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 461-474"},"PeriodicalIF":18.0,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}