Bioactive Materials最新文献_第6页

Semi-synthetic fibrous fibrin composites promote 3D microvascular assembly, survival, and host integration of endothelial cells without mesenchymal cell support 半合成纤维蛋白复合材料促进三维微血管组装，存活，和宿主整合内皮细胞没有间充质细胞的支持

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-03 DOI: 10.1016/j.bioactmat.2025.02.029

Firaol S. Midekssa , Christopher D. Davidson , Megan E. Wieger , Jordan L. Kamen , Kaylin M. Hanna , Danica Kristen P. Jayco , Michael M. Hu , Nicole E. Friend , Andrew J. Putnam , Adam S. Helms , Ariella Shikanov , Brendon M. Baker

{"title":"Semi-synthetic fibrous fibrin composites promote 3D microvascular assembly, survival, and host integration of endothelial cells without mesenchymal cell support","authors":"Firaol S. Midekssa , Christopher D. Davidson , Megan E. Wieger , Jordan L. Kamen , Kaylin M. Hanna , Danica Kristen P. Jayco , Michael M. Hu , Nicole E. Friend , Andrew J. Putnam , Adam S. Helms , Ariella Shikanov , Brendon M. Baker","doi":"10.1016/j.bioactmat.2025.02.029","DOIUrl":"10.1016/j.bioactmat.2025.02.029","url":null,"abstract":"<div><div>Vasculogenic assembly of 3D capillary networks remains a promising approach to vascularizing tissue-engineered grafts, a significant outstanding challenge in tissue engineering and regenerative medicine. Current approaches for vasculogenic assembly rely on the inclusion of supporting mesenchymal cells alongside endothelial cells, co-encapsulated within vasculo-conducive materials such as low-density fibrin hydrogels. Here, we established a material-based approach to circumvent the need for supporting mesenchymal cells and report that the inclusion of synthetic matrix fibers in dense (>3 mg mL<sup>-1</sup>) 3D fibrin hydrogels can enhance vasculogenic assembly in endothelial cell monocultures. Surprisingly, we found that the addition of non-cell-adhesive synthetic matrix fibers compared to cell-adhesive synthetic fibers best encouraged vasculogenic assembly, proliferation, lumenogenesis, a vasculogenic transcriptional program, and additionally promoted cell-matrix interactions and intercellular force transmission. Implanting fiber-reinforced prevascularized constructs to assess graft-host vascular integration, we demonstrate additive effects of enhanced vascular network assembly during <em>in vitro</em> pre-culture, fiber-mediated improvements in endothelial cell survival and vascular maintenance post-implantation, and enhanced host cell infiltration that collectively enabled graft vessel integration with host circulation. This work establishes synthetic matrix fibers as an inexpensive alternative to sourcing and expanding secondary supporting cell types for the prevascularization of tissue constructs.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"49 ","pages":"Pages 652-669"},"PeriodicalIF":18.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotherapeutic strategies exploiting biological traits of cancer stem cells 利用肿瘤干细胞生物学特性的纳米治疗策略

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-03 DOI: 10.1016/j.bioactmat.2025.03.016

Hongyu Wang , Wenjing Zhang , Yun Sun , Xican Xu , Xiaoyang Chen , Kexu Zhao , Zhao Yang , Huiyu Liu

{"title":"Nanotherapeutic strategies exploiting biological traits of cancer stem cells","authors":"Hongyu Wang , Wenjing Zhang , Yun Sun , Xican Xu , Xiaoyang Chen , Kexu Zhao , Zhao Yang , Huiyu Liu","doi":"10.1016/j.bioactmat.2025.03.016","DOIUrl":"10.1016/j.bioactmat.2025.03.016","url":null,"abstract":"<div><div>Cancer stem cells (CSCs) represent a distinct subpopulation of cancer cells that orchestrate cancer initiation, progression, metastasis, and therapeutic resistance. Despite advances in conventional therapies, the persistence of CSCs remains a major obstacle to achieving cancer eradication. Nanomedicine-based approaches have emerged for precise CSC targeting and elimination, offering unique advantages in overcoming the limitations of traditional treatments. This review systematically analyzes recent developments in nanomedicine for CSC-targeted therapy, emphasizing innovative nanomaterial designs addressing CSC-specific challenges. We first provide a detailed examination of CSC biology, focusing on their surface markers, signaling networks, microenvironmental interactions, and metabolic signatures. On this basis, we critically evaluate cutting-edge nanomaterial engineering designed to exploit these CSC traits, including stimuli-responsive nanodrugs, nanocarriers for drug delivery, and multifunctional nanoplatforms capable of generating localized hyperthermia or reactive oxygen species. These sophisticated nanotherapeutic approaches enhance selectivity and efficacy in CSC elimination, potentially circumventing drug resistance and cancer recurrence. Finally, we present an in-depth analysis of current challenges in translating nanomedicine-based CSC-targeted therapies from bench to bedside, offering critical insights into future research directions and clinical implementation. This review aims to provide a comprehensive framework for understanding the intersection of nanomedicine and CSC biology, contributing to more effective cancer treatment modalities.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 61-94"},"PeriodicalIF":18.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suprachoroidal space-inducing hydrogel-forming microneedles (SI-HFMN): An innovative platform for drug delivery to the posterior segment of the eye 脉络膜上空间诱导水凝胶形成微针（SI-HFMN）：一种创新的药物输送到眼睛后段的平台

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-03 DOI: 10.1016/j.bioactmat.2025.03.024

Jaibyung Choi , Suhyeon Shim , Jiwoo Shin , Ahhyun Lee , Jaan Strang , Tobias Braun , Reto Naef , Hyungil Jung

{"title":"Suprachoroidal space-inducing hydrogel-forming microneedles (SI-HFMN): An innovative platform for drug delivery to the posterior segment of the eye","authors":"Jaibyung Choi , Suhyeon Shim , Jiwoo Shin , Ahhyun Lee , Jaan Strang , Tobias Braun , Reto Naef , Hyungil Jung","doi":"10.1016/j.bioactmat.2025.03.024","DOIUrl":"10.1016/j.bioactmat.2025.03.024","url":null,"abstract":"<div><div>The suprachoroidal space (SCS), which exists between the sclera and choroid, offers a promising delivery route to the posterior segment of the eye (PSE) and is integrated with hollow microneedles (HMNs) for minimally invasive delivery. However, HMNs are limited by backflow owing to their narrow channel. Therefore, this study proposes a biocompatible SCS-inducing hydrogel-forming microneedle (SI-HFMN) with a specially designed candlelit shape that swells to separate the sclera from the choroid. The induced SCS provides a route for delivering loaded drugs to the PSE upon application. The optimized formulation of 20 % (w/w) poly(methyl vinyl ether-alt-maleic acid) (PMVE/MA) crosslinked with 7.5 % (w/w) polyethylene glycol (PEG) possesses sufficient mechanical strength (5.1 ± 0.7 N) to penetrate both the sclera and swell by 356 ± 28 %, to mechanically stimulate SCS formation. The formulation also recorded a drug absorption amount of 101 ± 9 μg/mg of hydrogel. Furthermore, <em>in vitro</em> and <em>ex vivo</em> experiments demonstrated the ability of the SI-HFMN to deliver drugs to the PSE via the formed SCS. Thus, this system offers an innovative method for drug delivery to PSE by inducing SCS formation.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 47-60"},"PeriodicalIF":18.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mytilus edulis foot protein mimics for tailoring long-acting endothelium-mimicking anti-thrombotic surfaces Mytilus edulis足蛋白模拟物用于定制长效内皮模拟抗血栓表面

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-01 DOI: 10.1016/j.bioactmat.2025.03.019

Zeyu Du , Yuting Huang , Qing Ma , Wentai Zhang , Yan Fu , Nan Huang , Xin Li , Zhilu Yang , Wenjie Tian

{"title":"Mytilus edulis foot protein mimics for tailoring long-acting endothelium-mimicking anti-thrombotic surfaces","authors":"Zeyu Du , Yuting Huang , Qing Ma , Wentai Zhang , Yan Fu , Nan Huang , Xin Li , Zhilu Yang , Wenjie Tian","doi":"10.1016/j.bioactmat.2025.03.019","DOIUrl":"10.1016/j.bioactmat.2025.03.019","url":null,"abstract":"<div><div>Surfaces with enduring and superior antithrombotic properties are essential for long-term blood-contacting devices. While current surface engineering strategies integrating anticoagulants and antiplatelet agents show promise in mimicking the non-thrombogenic properties of the endothelium, their long-term effectiveness remains limited. Here, we report an easy-to-perform, dual-biomimetic surface engineering strategy for tailoring long-acting endothelium-mimicking anti-thrombotic surfaces. We first designed a <em>Mytilus edulis</em> foot protein-5 (Mefp-5) mimic rich in amine and clickable alkynyl groups to polymerize-deposit a chemical robust coating onto the surface through a mussel-inspired adhesion mechanism. Then, a clickable nitric oxide (NO, an antiplatelet agent)-generating enzyme and the anticoagulant heparin were sequentially co-grafted onto the chemical robust coatings via click chemistry and carbodiimide chemistry. Our results demonstrate that this engineered surface achieved an impressive NO catalytic release efficiency of up to 88 %, while heparin retained 86 % of its bioactivity even after one month of exposure to PBS containing NO donor. Both <em>in vitro</em> and <em>in vivo</em> experiments confirmed that this robust endothelium-mimicking coating substantially reduces thrombosis formation. Overall, our long-acting endothelium-mimicking anti-thrombotic coatings present a promising and feasible strategy to address thrombosis-related challenges associated with blood-contacting devices.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 1-13"},"PeriodicalIF":18.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclodextrins as multifunctional tools for advanced biomaterials in tissue repair and regeneration 环糊精作为先进生物材料在组织修复和再生中的多功能工具

IF 18 1区医学

Bioactive Materials Pub Date : 2025-03-27 DOI: 10.1016/j.bioactmat.2025.03.018

Yu Bin Lee , Mi-Lang Kyun , Young Ju Lee , Hye-Eun Shim , Kang Moo Huh , Sun-Woong Kang

{"title":"Cyclodextrins as multifunctional tools for advanced biomaterials in tissue repair and regeneration","authors":"Yu Bin Lee , Mi-Lang Kyun , Young Ju Lee , Hye-Eun Shim , Kang Moo Huh , Sun-Woong Kang","doi":"10.1016/j.bioactmat.2025.03.018","DOIUrl":"10.1016/j.bioactmat.2025.03.018","url":null,"abstract":"<div><div>Cyclodextrins (CDs), characterized by their unique cyclic oligosaccharide structure and exceptional capacity for molecular encapsulation through host-guest interactions, have garnered significant attention as versatile building blocks in advanced biomaterials. This review explores the application of CD-based biomaterials in tissue engineering and regenerative medicine, emphasizing their synthesis, physicochemical characterization, biocompatibility, and translational potential. Special emphasis is placed on the development of CD-modified hydrogels, their interaction with host-guest molecules, and their use in advanced therapeutic strategies. Additionally, we discuss various fabrication methods for CD-based biomaterials, including physical and covalent crosslinking, self-assembly, and enzymatic crosslinking, each providing unique properties suited for advanced therapeutic strategies. Safety considerations and potential regulatory challenges associated with these materials are also examined. By providing a comprehensive overview of recent advancements, this review aims to highlight the promising role of cyclodextrins as multifunctional tools in enhancing tissue repair and regeneration, paving the way for innovative therapeutic solutions in clinical settings.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"49 ","pages":"Pages 627-651"},"PeriodicalIF":18.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous mechanical-gradient hydrogel with on-demand distributed Mn2+/Mg-doped hydroxyapatite@Fe3O4 for functional osteochondral regeneration 连续机械梯度水凝胶，按需分布Mn2+/ mg掺杂hydroxyapatite@Fe3O4用于功能性骨软骨再生

IF 18 1区医学

Bioactive Materials Pub Date : 2025-03-27 DOI: 10.1016/j.bioactmat.2025.03.013

Junwei Xu , Yi Cui , Ping Li , Xuemei Sun , Zhiheng Chen , Jingxi Wang , Xuenan Gu , Xiaogang Wang , Yubo Fan

{"title":"Continuous mechanical-gradient hydrogel with on-demand distributed Mn2+/Mg-doped hydroxyapatite@Fe3O4 for functional osteochondral regeneration","authors":"Junwei Xu , Yi Cui , Ping Li , Xuemei Sun , Zhiheng Chen , Jingxi Wang , Xuenan Gu , Xiaogang Wang , Yubo Fan","doi":"10.1016/j.bioactmat.2025.03.013","DOIUrl":"10.1016/j.bioactmat.2025.03.013","url":null,"abstract":"<div><div>Traditional layered gradient scaffolds are susceptible to delamination owing to abrupt stress alterations, thereby rendering them inefficacious for the integrated repair of osteochondral defects. This study proposed a novel hydrogel possessing continuous magnetic-mechanical and multiple functional metal elements gradients. The establishment of these gradients within the hydrogel was accomplished by first applying a magnetic field to FMHM particles (Fe<sub>3</sub>O<sub>4</sub> deposited with Mg-doped hydroxyapatite (MgHA@Fe<sub>3</sub>O<sub>4</sub>) and grafted with γ-(methacryloyloxy) propyl trimethoxysilane) dispersed in poly (ethylene glycol) diacrylate/sodium alginate solution to create a gradient, followed by thermal polymerization to achieve the magnetic and mechanical gradients. Subsequently secondary crosslinking with Mn<sup>2+</sup> realized the gradient distribution of Mn<sup>2+</sup> which was reverse to the gradient of MgHA@Fe<sub>3</sub>O<sub>4</sub>. The on-demand gradient distributions of Mn<sup>2+</sup> and MgHA@Fe<sub>3</sub>O<sub>4</sub> enhanced cartilage and osteogenic differentiation of bone marrow-derived mesenchymal stem cells, respectively. The continuous gradient hydrogel attained remarkable repair effects on full-thickness osteochondral defects in rat knee joints. Its capacity to foster the growth of both cartilage and subchondral bone may be associated with the fact that the mechanical gradient modulated the gradient nuclear localization and expression of the mechanosensitive factor Yes-associated protein 1. With stiffness and magnetism gradients, along with the on-demand synergistic impacts of multi-gradient metal elements Mn-Fe/Mg/Ca, this hydrogel presents a prospective option for the regeneration of tissues/interface tissues exhibiting physiological gradients.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"49 ","pages":"Pages 608-626"},"PeriodicalIF":18.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of electrical and chemical cues with biodegradable scaffolds for large peripheral nerve defect regeneration 电和化学线索与生物可降解支架在大周围神经缺损再生中的协同作用

IF 18 1区医学

Bioactive Materials Pub Date : 2025-03-26 DOI: 10.1016/j.bioactmat.2025.03.017

Rosalie Bordett , Sama Abdulmalik , Allen Zennifer , Suranji Wijekoon , Sai Sadhananth Srinivasan , Ergin Coskun , Yeshavanth Kumar Banasavadi Siddegowda , Xiaojun Yu , Sangamesh G. Kumbar

{"title":"Synergistic effects of electrical and chemical cues with biodegradable scaffolds for large peripheral nerve defect regeneration","authors":"Rosalie Bordett , Sama Abdulmalik , Allen Zennifer , Suranji Wijekoon , Sai Sadhananth Srinivasan , Ergin Coskun , Yeshavanth Kumar Banasavadi Siddegowda , Xiaojun Yu , Sangamesh G. Kumbar","doi":"10.1016/j.bioactmat.2025.03.017","DOIUrl":"10.1016/j.bioactmat.2025.03.017","url":null,"abstract":"<div><div>Large-gap peripheral nerve injuries (PNI) are often treated with autografts, allografts, or synthetic grafts to facilitate nerve regeneration, but these options are often limited in their availability or functionality. To address these issues, we developed ionically conductive (IC) nerve guidance conduits (NGCs) of sufficient biodegradability, mechanical strength, and bioactivity to support large-gap nerve regeneration. These chitosan-based NGCs release 4-aminopyridine (4-AP) from embedded halloysite nanotubes, and the NGC's IC properties enable transcutaneous electrical stimulation (ES) without invasive electrodes. <em>In vitro</em>, we found scaffolds with ES+4-AP synergistically enhanced Schwann cell adhesion, proliferation, and neurotrophin secretion, significantly improving axonal growth and neurite extension. <em>In vivo</em>, these scaffolds in large-gap PNI boosted neurotrophin levels, myelination, nerve function, and muscle weight while promoting angiogenesis and reducing fibrosis. Upregulated Trk receptors and PI3K/Akt and MAPK pathway highlight the regenerative potential. This study advances understanding of ES-mediated regeneration and supports innovative strategies for nerve and musculoskeletal repair.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"49 ","pages":"Pages 586-607"},"PeriodicalIF":18.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Minimally invasive snakebite inspired microneedle delivery system for internal organs 微创蛇咬伤启发的内部器官微针输送系统

IF 18 1区医学

Bioactive Materials Pub Date : 2025-03-24 DOI: 10.1016/j.bioactmat.2025.03.004

Xuan Mei , Dashuai Zhu , Junlang Li , Ke Huang , Shiqi Hu , Malcolm Xing , Ke Cheng

{"title":"Minimally invasive snakebite inspired microneedle delivery system for internal organs","authors":"Xuan Mei , Dashuai Zhu , Junlang Li , Ke Huang , Shiqi Hu , Malcolm Xing , Ke Cheng","doi":"10.1016/j.bioactmat.2025.03.004","DOIUrl":"10.1016/j.bioactmat.2025.03.004","url":null,"abstract":"<div><div>Efficient distribution of therapeutics to the targeted site, particularly internal organs, is essential for their therapeutic success. Here, we developed a therapeutic delivery system targeting internal organs, which features a mechanism akin to a snake's jaw for grasping and deploying detachable microneedles (MNs) embedded with therapeutics. This solves the current challenges of delivering microneedle patches without open chest or abdominal wall surgery. We showed an example of this technology via delivering exosomes derived from mesenchymal stem cells (MSCs) directly to the heart's damaged regions via percutaneous minimally invasive surgery. The shell of MNs is fabricated from methacrylated hyaluronic acid (MeHA), which ensures mechanical strength for myocardium penetration, while the hyaluronic acid (HA) core allows a sustained release of exosomes. In a rat model of myocardial infarction (MI), the delivery of exosomes-loaded microneedles (XOs-MNs) resulted in angiomyogenesis and promoted cardiac function. The feasibility of this microneedle delivery method was also confirmed in a pig model. With its capability to encapsulate a wide range of therapeutic formulations, our system presents a versatile platform for the minimally invasive administration of treatments to internal organs.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"49 ","pages":"Pages 576-585"},"PeriodicalIF":18.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprinting of Alginate-Norbornene bioinks to create a versatile platform for kidney in vitro modeling 海藻酸盐-降冰片烯生物链接的生物打印为肾脏体外建模创造了一个多功能平台

IF 18 1区医学

Bioactive Materials Pub Date : 2025-03-22 DOI: 10.1016/j.bioactmat.2025.03.010

Francesca Perin , Anna Ricci , Sveva Fagiolino , Aleksandra Rak-Raszewska , Helen Kearney , Jopeth Ramis , Ivo Bereen , Matthew Baker , Devid Maniglio , Antonella Motta , Lorenzo Moroni , Carlos Mota

{"title":"Bioprinting of Alginate-Norbornene bioinks to create a versatile platform for kidney in vitro modeling","authors":"Francesca Perin , Anna Ricci , Sveva Fagiolino , Aleksandra Rak-Raszewska , Helen Kearney , Jopeth Ramis , Ivo Bereen , Matthew Baker , Devid Maniglio , Antonella Motta , Lorenzo Moroni , Carlos Mota","doi":"10.1016/j.bioactmat.2025.03.010","DOIUrl":"10.1016/j.bioactmat.2025.03.010","url":null,"abstract":"<div><div>Chronic kidney diseases affect a significant portion of the global population and their prevalence is expected to increase in the coming years. Advanced <em>in vitro</em> models are crucial for understanding disease onset and for improving drug testing. Emerging strategies have enhanced the accuracy of these models by incorporating 3D culture and perfusion systems. Notably, efforts have focused on modeling the nephron, particularly endothelialized and epithelialized tubular structures, with perfusion to simulate toxin exchange for nephrotoxicity testing. New approaches combining biomaterials with patient-derived kidney epithelial cells show promise for high-throughput personalized drug screening. However, these methods often rely on decellularized extracellular matrix materials, such as Matrigel® and collagen, which suffer from batch-to-batch variability. To address reproducibility issues, we used norbornene-functionalized alginate to produce peptide-functionalized thiol-ene crosslinked hydrogels. By varying the composition of crosslinkers and peptide functionalization, we tuned the cell interaction with the hydrogels. The rapid reaction kinetics enabled the bioprinting of cell-laden tubular structures using microfluidic bioprinting, without the need for ionic crosslinking, by adapting the printer with UV irradiation at the nozzle. The bioprinted fibers successfully formed monolayers, indicating their potential for creating advanced kidney <em>in vitro</em> models. Thiol-ene crosslinked hydrogels proved to be highly tunable and adaptable to microfluidic bioprinting, demonstrating significant promise for further application to create kidney <em>in vitro</em> models.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"49 ","pages":"Pages 550-563"},"PeriodicalIF":18.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Two decades of continuous progresses and breakthroughs in the field of bioactive ceramics and glasses driven by CICECO-hub scientists” [Bioact. Mater. 40 (2024) 104–147] “在CICECO-hub科学家的推动下，生物活性陶瓷和玻璃领域20年的不断进步和突破”[Bioact.]Mater. 40 (2024) 104-147]

IF 18 1区医学

Bioactive Materials Pub Date : 2025-03-22 DOI: 10.1016/j.bioactmat.2025.02.044

H.R. Fernandes , S. Kannan , M. Alam , G.E. Stan , A.C. Popa , R. Buczyński , P. Gołębiewski , J.M.F. Ferreira

引用次数: 0