Bioactive Materials最新文献

{"title":"Progress in enzyme-powered micro/nanomotors in diagnostics and therapeutics","authors":"Jinpeng Zhao ,&nbsp;Banghui Wang ,&nbsp;Mingzhe Yan ,&nbsp;Yuxin Liu ,&nbsp;Ruizhe Zhao ,&nbsp;Xuezhe Wang ,&nbsp;Tianyi Shao ,&nbsp;Yifei Li ,&nbsp;Muhammad Imran ,&nbsp;Mingze Ji ,&nbsp;Hong Zhao ,&nbsp;Carlos F. Guimarães ,&nbsp;Guotai Li ,&nbsp;Qihui Zhou ,&nbsp;Rui L. Reis","doi":"10.1016/j.bioactmat.2024.12.022","DOIUrl":"10.1016/j.bioactmat.2024.12.022","url":null,"abstract":"<div><div>Enzyme-powered micro/nanomotors (EMNMs) represent cutting-edge research taking advantage of enzymes as biocatalysts to provide a driving force for micro/nanomotors. Up to now, EMNMs have been designed to be powered by catalase, urease, lipase, collagenase, compound enzymes, <em>etc</em>. They not only have good biocompatibility and biosafety but also possess the unique ability to utilize physiologically relevant fuel to achieve autonomous propulsion through <em>in vivo</em> catalytic reactions. This innovation has opened exciting possibilities for medical applications of EMNMs. Given the fact that the human body is naturally abundant with substrates available for enzymatic reactions, EMNMs can effectively exploit the complex microenvironment associated with diseases, enabling the diagnosis and treatment of various medical conditions. In this review, we first introduce different kinds of EMNMs applied in specific environments for the diagnosis and treatment of diseases, while highlighting their advancements for revolutionizing healthcare practices. Then, we address the challenges faced in this rapidly evolving field, and at last, the potential future development directions are discussed. As the potential of EMNMs becomes increasingly evident, continued research and exploration are essential to unlock their full capabilities and to ensure their successful integration into clinical applications.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 555-568"},"PeriodicalIF":18.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cancer vaccine designed from homologous ferritin-based fusion protein with enhanced DC-T cell crosstalk for durable adaptive immunity against tumors","authors":"Jun Wu ,&nbsp;Jing Liang ,&nbsp;Sichen Li ,&nbsp;Jinjin Lu ,&nbsp;Yi Li ,&nbsp;Bin Zhang ,&nbsp;Min Gao ,&nbsp;Juan Zhou ,&nbsp;Yan Zhang ,&nbsp;Jinghua Chen","doi":"10.1016/j.bioactmat.2024.12.029","DOIUrl":"10.1016/j.bioactmat.2024.12.029","url":null,"abstract":"<div><div>Peptide vaccines based on tumor antigens face the challenges of rapid clearance of peptides, low immunogenicity, and immune suppressive tumor microenvironment. However, the traditional solution mainly uses exogenous substances as adjuvants or carriers to enhance innate immune responses, but excessive inflammation can damage adaptive immunity. In the current study, we propose a straightforward novel nanovaccine strategy by employing homologous human ferritin light chain for minimized innate immunity and dendritic cell (DC) targeting, the cationic KALA peptide for enhanced cellular uptake, and suppressor of cytokine signaling 1 (SOCS1) siRNA for modulating DC activity. Upon fusing with the KALA peptide, this nanovaccine presents as a novel 40-mer cage structure, with highly enriched antigen peptides of proper size (25 nm) for targeted delivery to lymph nodes. The loading of SOCS1 siRNA onto the KALA peptide promoted DC maturation in tumor environment, leading to a 3-fold increase in antigen presentation compared to alum adjuvant. Moreover, it demonstrates remarkable efficacy in suppressing tumor progression and metastasis, together with prolonged survival. In addition, the nanovaccine stimulates up to 40 % memory T cells, thereby achieving sustained protection against tumor re-challenge. This unprecedented nanovaccine platform can ignite fresh interdisciplinary discussions on interactive strategies for future peptide vaccine development.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 516-530"},"PeriodicalIF":18.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual physiological responsive structural color hydrogel particles for wound repair","authors":"Li Wang ,&nbsp;Lu Fan ,&nbsp;Anne M. Filppula ,&nbsp;Yu Wang ,&nbsp;Feika Bian ,&nbsp;Luoran Shang ,&nbsp;Hongbo Zhang","doi":"10.1016/j.bioactmat.2025.01.002","DOIUrl":"10.1016/j.bioactmat.2025.01.002","url":null,"abstract":"<div><div>Hydrogel-based patches have demonstrated their values in diabetic wounds repair, particularly those intelligent dressings with continuous repair promoting and monitoring capabilities. Here, we propose a type of dual physiological responsive structural color particles for wound repair. The particles are composed of a hyaluronic acid methacryloyl (HAMA)-sodium alginate (Alg) inverse opal scaffold, filled with oxidized dextran (ODex)/quaternized chitosan (QCS) hydrogel. The photo-polymerized HAMA and ionically cross-linked Ca-Alg constitute to the dual-network hydrogel with stable structural color. Furthermore, the ODex/QCS hydrogel, combined with glucose oxidase (GOX), exhibits pH/glucose dual responsiveness. Moreover, antimmicrobial peptide (AMP) plus vascular endothelial growth factor (VEGF) are comprised within the GOX-doped ODex/QCS hydrogel. In the high-glucose wound environment, GOX catalyzes glucose to generate acidic products, triggering rapid release of AMP and VEGF. Importantly, this process also leads to structural color changes of the particles, offering significant potential for wound monitoring. It has been demonstrated that such particles greatly promote the healing progress of diabetic wound <em>in vivo</em>. These results indicate that the present dual responsive particles would find valuable applications in diabetic wounds repair and the associated areas.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 494-502"},"PeriodicalIF":18.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11760816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered bacillus subtilis enhances bone regeneration via immunoregulation and anti-Infection","authors":"Fang-Sheng Fu ,&nbsp;Huan-Huan Chen ,&nbsp;Yu Chen ,&nbsp;Ying Yuan ,&nbsp;Yong Zhao ,&nbsp;Aixi Yu ,&nbsp;Xian-Zheng Zhang","doi":"10.1016/j.bioactmat.2025.01.003","DOIUrl":"10.1016/j.bioactmat.2025.01.003","url":null,"abstract":"<div><div>Chronic osteomyelitis caused by implant infections is a common complication following orthopedic surgery. Preventing bacterial infection and simultaneously improving bone regeneration are the key for osteomyelitis. Current treatments include systemic antibiotics and multiple surgical interventions, but the strategies available for treatment are limited. In this study, a multifunctional engineered <em>Bacillus subtilis</em> (<em>B.</em> <em>sub</em>) hydrogel with sulfasalazine (SSZ) is developed to treat methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) infection and anti-inflammatory and promote bone regeneration. <em>B. sub</em> in alginate hydrogels protects <em>B. sub</em> from being cleared by the host immune system while allowing the release of its bioactive substances, including antibacterial peptides and anti-inflammatory agents such as SSZ. The results show that the engineered probiotic hydrogels exhibit excellent antibacterial efficacy against MRSA (97 %) and prevent the development of bacterial resistance. The antibacterial effect is primarily mediated through the secretion of bioactive peptides by <em>B. sub</em>, which not only inhibit MRSA growth but also reduce the likelihood of resistance development. Meanwhile, the probiotic hydrogel has a greater ability to induce M2 polarization of macrophages and promote angiogenesis, resulting in enhanced osteogenic differentiation in bone marrow mesenchymal stem cells (BMSCs) and thus enhancing bone regeneration. This engineered probiotic hydrogel offers a promising strategy by simultaneously combating bacterial infection and enhancing osteogenic differentiation for chronic osteomyelitis.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 503-515"},"PeriodicalIF":18.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11760808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bolstered bone regeneration by multiscale customized magnesium scaffolds with hierarchical structures and tempered degradation","authors":"Zehui Lv ,&nbsp;Bo Peng ,&nbsp;Yu Ye ,&nbsp;Haojing Xu ,&nbsp;Xuejie Cai ,&nbsp;Jinge Liu ,&nbsp;Jiabao Dai ,&nbsp;Yixin Bian ,&nbsp;Peng Wen ,&nbsp;Xisheng Weng","doi":"10.1016/j.bioactmat.2024.12.002","DOIUrl":"10.1016/j.bioactmat.2024.12.002","url":null,"abstract":"<div><div>Addressing irregular bone defects is a formidable clinical challenge, as traditional scaffolds frequently fail to meet the complex requirements of bone regeneration, resulting in suboptimal healing. This study introduces a novel 3D-printed magnesium scaffold with hierarchical structure (macro-, meso-, and nano-scales) and tempered degradation (microscale), intricately customized at multiple scales to bolster bone regeneration according to patient-specific needs. For the hierarchical structure, at the macroscale, it can feature anatomic geometries for seamless integration with the bone defect; The mesoscale pores are devised with optimized curvature and size, providing an adequate mechanical response as well as promoting cellular proliferation and vascularization, essential for natural bone mimicry; The nanoscale textured surface is enriched with a layered double hydroxide membrane, augmenting bioactivity and osteointegration. Moreover, microscale enhancements involve a dual-layer coating of high-temperature oxidized film and hydrotalcite, offering a robust shield against fast degradation. Eventually, this scaffold demonstrates superior geometrical characteristics, load-bearing capacity, and degradation performance, significantly outperforming traditional scaffolds based on in vitro and in vivo assessments, marking a breakthrough in repairing customized bone defects.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 457-475"},"PeriodicalIF":18.0,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11755084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanically regulated microcarriers with stem cell loading for skin photoaging therapy","authors":"Xiang Lin ,&nbsp;Anne M. Filppula ,&nbsp;Yuanjin Zhao ,&nbsp;Luoran Shang ,&nbsp;Hongbo Zhang","doi":"10.1016/j.bioactmat.2024.12.024","DOIUrl":"10.1016/j.bioactmat.2024.12.024","url":null,"abstract":"<div><div>Long-term exposure to ultraviolet radiation compromises skin structural integrity and results in disruption of normal physiological functions. Stem cells have gained attention in anti-photoaging, while controlling the tissue mechanical microenvironment of cell delivery sites is crucial for regulating cell fate and achieving optimal therapeutic performances. Here, we introduce a mechanically regulated human recombinant collagen (RHC) microcarrier generated through microfluidics, which is capable of modulating stem cell differentiation to treat photoaged skin. By controlling the cross-linking parameters, the mechanical properties of microcarriers could precisely tuned to optimize the stem cell differentiation. The microcarriers are surface functionalized with fibronectin (Fn)-platelet derived growth factor-BB (PDGF-BB) to facilitate adipose derived mesenchymal stem cells (Ad-MSCs) loading. In <em>in vivo</em> experiments, subcutaneous injection of stem cell loaded RHC microcarriers significantly reduced skin wrinkles after ultraviolet-injury, effectively promoted collagen synthesis, and increased vascular density. These encouraging results indicate that the present mechanically regulated microcarriers have great potential to deliver stem cells and regulate their differentiation for anti-photoaging treatments.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 448-456"},"PeriodicalIF":18.0,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen bonding-mediated phase-transition gelatin-based bioadhesives to regulate immune microenvironment for diabetic wound healing","authors":"Zhuoling Tian ,&nbsp;Ruoheng Gu ,&nbsp;Wenyue Xie ,&nbsp;Xing Su ,&nbsp;Zuoying Yuan ,&nbsp;Zhuo Wan ,&nbsp;Hao Wang ,&nbsp;Yaqian Liu ,&nbsp;Yuting Feng ,&nbsp;Xiaozhi Liu ,&nbsp;Jianyong Huang","doi":"10.1016/j.bioactmat.2024.12.014","DOIUrl":"10.1016/j.bioactmat.2024.12.014","url":null,"abstract":"<div><div>Gelatin-based biomaterials have emerged as promising candidates for bioadhesives due to their biodegradability and biocompatibility. However, they often face limitations due to the uncontrollable phase transition of gelatin, which is dominated by hydrogen bonds between peptide chains. Here, we developed controllable phase transition gelatin-based (CPTG) bioadhesives by regulating the dynamic balance of hydrogen bonds between the peptide chains using 2-hydroxyethylurea (HU) and punicalagin (PA). These CPTG bioadhesives exhibited significant enhancements in adhesion energy and injectability even at 4 °C compared to traditional gelatin bioadhesives. The developed bioadhesives could achieve self-reinforcing interfacial adhesion upon contact with moist wound tissues. This effect was attributed to HU diffusion, which disrupted the dynamic balance of hydrogen bonds and therefore induced a localized structural densification. This process was further facilitated by the presence of pyrogallol from PA. Furthermore, the CPTG bioadhesive could modulate the immune microenvironment, offering antibacterial, antioxidant, and immune-adjustable properties, thereby accelerating diabetic wound healing, as confirmed in a diabetic wound rat model. This proposed design strategy is not only crucial for developing controllable phase-transition bioadhesives for diverse applications, but also paves the way for broadening the potential applications of gelatin-based biomaterials.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 434-447"},"PeriodicalIF":18.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11755075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable thermosensitive antibiotic-laden chitosan hydrogel for regenerative endodontics","authors":"Alexandre Henrique dos Reis-Prado ,&nbsp;Maedeh Rahimnejad ,&nbsp;Renan Dal-Fabbro ,&nbsp;Priscila Toninatto Alves de Toledo ,&nbsp;Caroline Anselmi ,&nbsp;Pedro Henrique Chaves de Oliveira ,&nbsp;J. Christopher Fenno ,&nbsp;Luciano Tavares Angelo Cintra ,&nbsp;Francine Benetti ,&nbsp;Marco C. Bottino","doi":"10.1016/j.bioactmat.2024.12.026","DOIUrl":"10.1016/j.bioactmat.2024.12.026","url":null,"abstract":"<div><div>Injectable biomaterials, such as thermosensitive chitosan (CH)-based hydrogels, present a highly translational potential in dentistry due to their minimally invasive application, adaptability to irregular defects/shapes, and ability to carry therapeutic drugs. This work explores the incorporation of azithromycin (AZI) into thermosensitive CH hydrogels for use as an intracanal medication in regenerative endodontic procedures (REPs). The morphological and chemical characteristics of the hydrogel were assessed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and Fourier transform infrared spectroscopy (FTIR). The thermosensitivity, gelation kinetics, compressive strength, cytocompatibility, and antibacterial efficacy were evaluated according to well-established protocols. An <em>in vivo</em> model of periapical disease and evoked bleeding in rats' immature permanent teeth was performed to determine disinfection, tissue repair, and root formation. AZI was successfully incorporated into interconnected porous CH hydrogels, which retained their thermosensitivity. The mechanical and rheological findings indicated that adding AZI did not adversely affect the hydrogels’ strength and injectability. Incorporating 3 % and 5 % AZI into the hydrogels led to minimal cytotoxic effects compared to higher concentrations while enhancing the antibacterial response against endodontic bacteria. AZI-laden hydrogel significantly decreased <em>E. faecalis</em> biofilm compared to the controls. Regarding tissue response, the 3 % AZI-laden hydrogel improved mineralized tissue formation and vascularization compared to untreated teeth and those treated with double antibiotic paste. Our findings demonstrate that adding 3 % AZI into CH hydrogels ablates infection and supports neotissue formation <em>in vivo</em> when applied to a clinically relevant model of regenerative endodontics.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 406-422"},"PeriodicalIF":18.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"bFGF-Chitosan “brain glue” promotes functional recovery after cortical ischemic stroke","authors":"Jiao Mu ,&nbsp;Xiang Zou ,&nbsp;Xinjie Bao ,&nbsp;Zhaoyang Yang ,&nbsp;Peng Hao ,&nbsp;Hongmei Duan ,&nbsp;Wen Zhao ,&nbsp;Yudan Gao ,&nbsp;Jinting Wu ,&nbsp;Kun Miao ,&nbsp;Kwok-Fai So ,&nbsp;Liang Chen ,&nbsp;Ying Mao ,&nbsp;Xiaoguang Li","doi":"10.1016/j.bioactmat.2024.12.017","DOIUrl":"10.1016/j.bioactmat.2024.12.017","url":null,"abstract":"<div><div>The mammalian brain has an extremely limited ability to regenerate lost neurons and to recover function following ischemic stroke. A biomaterial strategy of slowly-releasing various regeneration-promoting factors to activate endogenous neurogenesis represents a safe and practical neuronal replacement therapy. In this study, basic fibroblast growth factor (bFGF)-Chitosan gel is injected into the stroke cavity. This approach promotes the proliferation of vascular endothelial cell, the formation of functional vascular network, and the final restoration of cerebral blood flow. Additionally, bFGF-Chitosan gel activates neural progenitor cells (NPCs) in the subventricular zone (SVZ), promotes the NPCs’ migration toward the stroke cavity and differentiation into mature neurons with diverse cell types (inhibitory gamma-aminobutyric acid neurons and excitatory glutamatergic neuron) and layer architecture (superficial cortex and deep cortex). These new-born neurons form functional synaptic connections with the host brain and reconstruct nascent neural networks. Furthermore, synaptogenesis in the stroke cavity and Nestin lineage cells respectively contribute to the improvement of sensorimotor function induced by bFGF-Chitosan gel after ischemic stroke. Lastly, bFGF-Chitosan gel inhibits microglia activation in the peri-infarct cortex. Our findings indicate that filling the stroke cavity with bFGF-Chitosan “brain glue” promotes angiogenesis, endogenous neurogenesis and synaptogenesis to restore function, offering innovative ideas and methods for the clinical treatment of ischemic stroke.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 386-405"},"PeriodicalIF":18.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11755050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bio-printed proteinaceous bioactive scaffold loaded with dual growth factor enhanced chondrogenesis and in situ cartilage regeneration","authors":"Prayas Chakma Shanto ,&nbsp;Seongsu Park ,&nbsp;Md Abdullah Al Fahad ,&nbsp;Myeongki Park ,&nbsp;Byong-Taek Lee","doi":"10.1016/j.bioactmat.2024.12.021","DOIUrl":"10.1016/j.bioactmat.2024.12.021","url":null,"abstract":"<div><div>Articular cartilage has a limited self-healing capacity, leading to joint degeneration and osteoarthritis over time. Therefore, bioactive scaffolds are gaining attention as a promising approach to regenerating and repairing damaged articular cartilage through tissue engineering. In this study, we reported on a novel 3D bio-printed proteinaceous bioactive scaffolds combined with natural porcine cancellous bone dECM, tempo-oxidized cellulose nanofiber (TOCN), and alginate carriers for TGF-β1, FGF-18, and ADSCs to repair cartilage defects. The characterization results demonstrate that the 3D scaffolds are physically stable and facilitate a controlled dual release of TGF-β1 and FGF-18. Moreover, the key biological proteins within the bioactive scaffold actively interact with the biological systems to create a favorable microenvironment for cartilage regeneration. Importantly, the <em>in vitro</em>, <em>in vivo</em>, and in silico simulation showed that the scaffolds promote stem cell recruitment, migration, proliferation, and ECM deposition, and synergistic effects of TGF-β1/FGF-18 with the bioactive scaffolds significantly regulate stem cell chondrogenesis by activating the PI3K/AKT and TGFβ1/Smad4 signaling pathways. After implantation, the proteinaceous bioactive scaffold led to the regeneration of mechanically robust, full-thickness cartilage tissue that closely resembles native cartilage. Thus, these findings may provide a promising approach for regulating stem cell chondrogenesis and treating in situ cartilage regeneration.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 365-385"},"PeriodicalIF":18.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}