Bioactive Materials最新文献

An engineered M2 macrophage-derived exosomes-loaded electrospun biomimetic periosteum promotes cell recruitment, immunoregulation, and angiogenesis in bone regeneration

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-05 DOI: 10.1016/j.bioactmat.2025.03.027

Zhuohao Wen , Shuyi Li , Yi Liu , Xueyan Liu , Huiguo Qiu , Yuejuan Che , Liming Bian , Miao Zhou

{"title":"An engineered M2 macrophage-derived exosomes-loaded electrospun biomimetic periosteum promotes cell recruitment, immunoregulation, and angiogenesis in bone regeneration","authors":"Zhuohao Wen , Shuyi Li , Yi Liu , Xueyan Liu , Huiguo Qiu , Yuejuan Che , Liming Bian , Miao Zhou","doi":"10.1016/j.bioactmat.2025.03.027","DOIUrl":"10.1016/j.bioactmat.2025.03.027","url":null,"abstract":"<div><div>The periosteum, a fibrous tissue membrane covering bone surfaces, is critical to osteogenesis and angiogenesis in bone reconstruction. Artificial periostea have been widely developed for bone defect repair, but most of these are lacking of periosteal bioactivity. Herein, a biomimetic periosteum (termed PEC-Apt-NP-Exo) is prepared based on an electrospun membrane combined with engineered exosomes (Exos). The electrospun membrane is fabricated using poly(ε-caprolactone) (core)-periosteal decellularized extracellular matrix (shell) fibers via coaxial electrospinning, to mimic the fibrous structure, mechanical property, and tissue microenvironment of natural periosteum. The engineered Exos derived from M2 macrophages are functionalized by surface modification of bone marrow mesenchymal stem cell (BMSC)-specific aptamers to further enhance cell recruitment, immunoregulation, and angiogenesis in bone healing. The engineered Exos are covalently bonded to the electrospun membrane, to achieve rich loading and long-term effects of Exos. <em>In vitro</em> experiments demonstrate that the biomimetic periosteum promotes BMSC migration and osteogenic differentiation via Rap1/PI3K/AKT signaling pathway, and enhances vascular endothelial growth factor secretion from BMSCs to facilitate angiogenesis. <em>In vivo</em> studies reveal that the biomimetic periosteum promotes new bone formation in large bone defect repair by inducing M2 macrophage polarization, endogenous BMSC recruitment, osteogenic differentiation, and vascularization. This research provides valuable insights into the development of a multifunctional biomimetic periosteum for bone regeneration.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 95-115"},"PeriodicalIF":18.0,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elytra-inspired zirconium phosphate nanonetwork: Toward high-quality osseointegration and physical-chemical-mechanical bond at the interface for zirconia-based dental materials

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-04 DOI: 10.1016/j.bioactmat.2025.03.028

Shuyi Wu , Yingyue Sun , Qihong Zhang , Wen Si , Peng Gao , Lei Lu , Zhennan Deng , Lihua Xu , Xinkun Shen , Jinsong Liu

{"title":"Elytra-inspired zirconium phosphate nanonetwork: Toward high-quality osseointegration and physical-chemical-mechanical bond at the interface for zirconia-based dental materials","authors":"Shuyi Wu , Yingyue Sun , Qihong Zhang , Wen Si , Peng Gao , Lei Lu , Zhennan Deng , Lihua Xu , Xinkun Shen , Jinsong Liu","doi":"10.1016/j.bioactmat.2025.03.028","DOIUrl":"10.1016/j.bioactmat.2025.03.028","url":null,"abstract":"<div><div>Yttria-stabilized zirconia (YSZ) is widely used in dental implants and prostheses due to its excellent aesthetic and restorative properties. However, its bio-inert surface limits early osseointegration and weakens bonding strengths with porcelain veneer/resin cement. Inspired by the structure of beetle elytra, this work proposes a novel strategy involving a self-assembled trabecular-honeycomb biomimetic zirconium phosphate (ZrP) nanonetwork to modify YSZ surfaces. This approach simultaneously enhances energy dissipation, interfacial bonding, and osseointegration. The pore size of ZrP nanonetwork was precisely controlled by adjusting reaction temperatures (120 °C and 160 °C) and phosphoric acid concentrations (1.0 wt% and 2.5 wt%). Compared to conventional YSZ, the ZrP nanonetworks achieved remarkable improvements in bond strength, showing increases of 111 % with porcelain veneer and 336 % with resin cement. These enhancements are attributed to multiscale physical-chemical-mechanical interactions, including micromechanical anchoring, chemical bonding via phosphate groups, and energy dissipation through topological optimization. <em>In vitro</em> studies demonstrated that large-pore-size nanonetworks promote osteogenic differentiation of osteoblasts and modulate macrophage polarization toward the M2 phenotype, fostering an immune environment conducive to bone regeneration. <em>In vivo</em> experiments further validated the superior osseointegration and bone regeneration capacities of the large-pore-size ZrP nanonetwork. Collectively, this biomimetic ZrP nanonetwork-modified YSZ, with its exceptional physical-chemical-mechanical bonding properties, osseointegration potential, and immune-modulating capabilities, represents a groundbreaking advancement in zirconia-based material for dental implants and prostheses.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 116-133"},"PeriodicalIF":18.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Janus piezoelectric adhesives regulate macrophage TRPV1/Ca2+/cAMP axis to stimulate tendon-to-bone healing by multi-omics analysis

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-04 DOI: 10.1016/j.bioactmat.2025.03.029

Moran Huang , Wan Li , Yaying Sun , Jize Dong , Chaojing Li , Henjie Jia , Yongjie Jiao , Lu Wang , Shanxing Zhang , Fujun Wang , Jiwu Chen

{"title":"Janus piezoelectric adhesives regulate macrophage TRPV1/Ca2+/cAMP axis to stimulate tendon-to-bone healing by multi-omics analysis","authors":"Moran Huang , Wan Li , Yaying Sun , Jize Dong , Chaojing Li , Henjie Jia , Yongjie Jiao , Lu Wang , Shanxing Zhang , Fujun Wang , Jiwu Chen","doi":"10.1016/j.bioactmat.2025.03.029","DOIUrl":"10.1016/j.bioactmat.2025.03.029","url":null,"abstract":"<div><div>Piezoelectric stimulation has garnered substantial interest as a promising strategy for tissue regeneration. However, studies investigating its impact on tendon-to-bone healing characterized by fibrocartilage remain scarce. Moreover, there are considerable technical challenges in achieving minimally invasive application of piezoelectric stimulation on the irregular tendon-to-bone interface. Herein, we developed Janus asymmetric piezoelectric adhesives by assembling adhesive hydrogel (GAN) and non-adhesive hydrogel (GM) on each side of piezoelectric poly (L-lactic acid) nanofiber. Piezoelectric adhesives exhibited superior anti-inflammatory effects both <em>in vitro</em> and <em>ex vivo</em>. Notably, the transient receptor potential (TRP) ion channels, a class of versatile signaling molecules, are closely associated with the regulation of inflammation. This study demonstrated that piezoelectric stimulation promoted Ca<sup>2+</sup> influx through the activation of transient receptor potential vanilloid 1 (TRPV1), further enhancing cAMP signaling pathway in macrophages by RNA sequencing. Additionally, <em>in vivo</em> proteomic analysis revealed Arachidonic acid metabolism and TNF-α signaling pathway downregulation and VEGF signaling pathway upregulation in a rat rotator cuff repair model. Piezoelectric adhesives ultimately achieved inflammation alleviation, angiogenesis enhancement, and fibrocartilage regeneration promotion, improving the biomechanical strength of the enthesis. This study elucidated the mechanism by which piezoelectric stimulation regulated tendon-to-bone healing through multi-omics analysis. The piezoelectric adhesives hold promise as a convenient and effective strategy for enhancing tendon-to-bone healing in clinical practice.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 134-151"},"PeriodicalIF":18.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multifunctional hydrogel loaded with magnesium-doped bioactive glass-induced vesicle clusters enhances diabetic wound healing by promoting intracellular delivery of extracellular vesicles

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-03 DOI: 10.1016/j.bioactmat.2025.03.025

Zetao Wang , Zhipeng Sun , Shuangli Zhu , Zhihao Qin , Xiaohong Yin , Yilin Ding , Huichang Gao , Xiaodong Cao

{"title":"A multifunctional hydrogel loaded with magnesium-doped bioactive glass-induced vesicle clusters enhances diabetic wound healing by promoting intracellular delivery of extracellular vesicles","authors":"Zetao Wang , Zhipeng Sun , Shuangli Zhu , Zhihao Qin , Xiaohong Yin , Yilin Ding , Huichang Gao , Xiaodong Cao","doi":"10.1016/j.bioactmat.2025.03.025","DOIUrl":"10.1016/j.bioactmat.2025.03.025","url":null,"abstract":"<div><div>The treatment of diabetic wounds (DWs) poses a significant medical challenge. Mesenchymal stem cell-derived small extracellular vesicles (sEVs) have demonstrated potential in accelerating healing by delivering growth factors and microRNAs. However, the rapid clearance by the circulatory system limits their concentration and bioavailability within cells. This study employed magnesium-doped bioactive glass (MgBG) to autonomously program sEVs into a vesicle cluster (EPPM), which was subsequently incorporated into a hydrogel to create a comprehensive repair system that enhanced the delivery of both sEVs and MgBG, thereby promoting rapid healing of diabetic wounds. This hydrogel exhibited excellent injectable, self-healing and bioadhesive properties, making it an ideal physical barrier for DWs. In addition, the hydrogels also possessed photoresponsive properties that facilitated their bactericidal activity. The released EPPM significantly increased the intracellular uptake and accumulation of sEVs, with approximately 8.2-fold enhancement in macrophages and 16.7-fold in endothelial cells. The EPPM clusters efficiently induce macrophage M2 polarization, reduce inflammatory responses at the wound site, and recruit cells, thereby promoting angiogenesis and collagen deposition. This integrated repair system provided a new platform for the comprehensive treatment of diabetic wounds.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 30-46"},"PeriodicalIF":18.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic-covalent hybrid hydrogels with cartilaginous immune microenvironment temporally regulating meniscus regeneration

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-03 DOI: 10.1016/j.bioactmat.2025.03.026

Zhanyu Chang , Xinyue Ran , Yaru Chu , Bohui Li , Zhenlin Fan , Genke Li , Dan Li , Wenjie Ren , Yujie Hua , Guangdong Zhou

{"title":"Dynamic-covalent hybrid hydrogels with cartilaginous immune microenvironment temporally regulating meniscus regeneration","authors":"Zhanyu Chang , Xinyue Ran , Yaru Chu , Bohui Li , Zhenlin Fan , Genke Li , Dan Li , Wenjie Ren , Yujie Hua , Guangdong Zhou","doi":"10.1016/j.bioactmat.2025.03.026","DOIUrl":"10.1016/j.bioactmat.2025.03.026","url":null,"abstract":"<div><div>Meniscus is a crescent-shaped fibrocartilage tissue for providing structural congruence and absorbing mechanical forces. Currently, the development of material-guided regeneration medicine strategy has emerged as a promising alternative for meniscus treatment. However, it often presents more complex pathological conditions of immune-inflammatory responses, and thus inevitably causes a harsh microenvironment that extremely hinders fibrocartilage regeneration. Therefore, there is an urgent need to develop bioactive materials to achieve cartilaginous immunomodulatory throughout the whole regenerative periods. In this study, we develop a novel dynamic-covalent hybrid (<strong>DCH</strong>) hydrogel with cartilaginous immune microenvironment (<strong>CIME</strong>) to temporally regulate meniscus regeneration. By combining dynamic boronic ester crosslinking and covalent photopolymerization reactions, <strong>DCH</strong> hydrogels exhibit favorable injectability, self-healing, and tissue adhesion properties for practical operation. Furthermore, <strong>CIME</strong> is successfully created by the introduction of a temporally on-demand regulatory system: naproxen anti-inflammatory drugs are preferentially released to regulate M1/M2 macrophage polarization through PI3K/Akt/mTOR signaling pathway at early stage, while TGFβ3/CTGF growth factors are on-demand released to promote fibrochondrogenic differentiation of stem cells in the post-regulatory microenvironment at later stage. Finally, <em>in vivo</em> experiments demonstrate the satisfactory repair of meniscus cartilage defects in rabbits by activating the endogenous repair of stem cells homing based on our established cartilaginous immunomodulatory strategy.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 14-29"},"PeriodicalIF":18.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Semi-synthetic fibrous fibrin composites promote 3D microvascular assembly, survival, and host integration of endothelial cells without mesenchymal cell support

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-03 DOI: 10.1016/j.bioactmat.2025.02.029

Firaol S. Midekssa , Christopher D. Davidson , Megan E. Wieger , Jordan L. Kamen , Kaylin M. Hanna , Danica Kristen P. Jayco , Michael M. Hu , Nicole E. Friend , Andrew J. Putnam , Adam S. Helms , Ariella Shikanov , Brendon M. Baker

{"title":"Semi-synthetic fibrous fibrin composites promote 3D microvascular assembly, survival, and host integration of endothelial cells without mesenchymal cell support","authors":"Firaol S. Midekssa , Christopher D. Davidson , Megan E. Wieger , Jordan L. Kamen , Kaylin M. Hanna , Danica Kristen P. Jayco , Michael M. Hu , Nicole E. Friend , Andrew J. Putnam , Adam S. Helms , Ariella Shikanov , Brendon M. Baker","doi":"10.1016/j.bioactmat.2025.02.029","DOIUrl":"10.1016/j.bioactmat.2025.02.029","url":null,"abstract":"<div><div>Vasculogenic assembly of 3D capillary networks remains a promising approach to vascularizing tissue-engineered grafts, a significant outstanding challenge in tissue engineering and regenerative medicine. Current approaches for vasculogenic assembly rely on the inclusion of supporting mesenchymal cells alongside endothelial cells, co-encapsulated within vasculo-conducive materials such as low-density fibrin hydrogels. Here, we established a material-based approach to circumvent the need for supporting mesenchymal cells and report that the inclusion of synthetic matrix fibers in dense (>3 mg mL<sup>-1</sup>) 3D fibrin hydrogels can enhance vasculogenic assembly in endothelial cell monocultures. Surprisingly, we found that the addition of non-cell-adhesive synthetic matrix fibers compared to cell-adhesive synthetic fibers best encouraged vasculogenic assembly, proliferation, lumenogenesis, a vasculogenic transcriptional program, and additionally promoted cell-matrix interactions and intercellular force transmission. Implanting fiber-reinforced prevascularized constructs to assess graft-host vascular integration, we demonstrate additive effects of enhanced vascular network assembly during <em>in vitro</em> pre-culture, fiber-mediated improvements in endothelial cell survival and vascular maintenance post-implantation, and enhanced host cell infiltration that collectively enabled graft vessel integration with host circulation. This work establishes synthetic matrix fibers as an inexpensive alternative to sourcing and expanding secondary supporting cell types for the prevascularization of tissue constructs.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"49 ","pages":"Pages 652-669"},"PeriodicalIF":18.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotherapeutic strategies exploiting biological traits of cancer stem cells

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-03 DOI: 10.1016/j.bioactmat.2025.03.016

Hongyu Wang , Wenjing Zhang , Yun Sun , Xican Xu , Xiaoyang Chen , Kexu Zhao , Zhao Yang , Huiyu Liu

{"title":"Nanotherapeutic strategies exploiting biological traits of cancer stem cells","authors":"Hongyu Wang , Wenjing Zhang , Yun Sun , Xican Xu , Xiaoyang Chen , Kexu Zhao , Zhao Yang , Huiyu Liu","doi":"10.1016/j.bioactmat.2025.03.016","DOIUrl":"10.1016/j.bioactmat.2025.03.016","url":null,"abstract":"<div><div>Cancer stem cells (CSCs) represent a distinct subpopulation of cancer cells that orchestrate cancer initiation, progression, metastasis, and therapeutic resistance. Despite advances in conventional therapies, the persistence of CSCs remains a major obstacle to achieving cancer eradication. Nanomedicine-based approaches have emerged for precise CSC targeting and elimination, offering unique advantages in overcoming the limitations of traditional treatments. This review systematically analyzes recent developments in nanomedicine for CSC-targeted therapy, emphasizing innovative nanomaterial designs addressing CSC-specific challenges. We first provide a detailed examination of CSC biology, focusing on their surface markers, signaling networks, microenvironmental interactions, and metabolic signatures. On this basis, we critically evaluate cutting-edge nanomaterial engineering designed to exploit these CSC traits, including stimuli-responsive nanodrugs, nanocarriers for drug delivery, and multifunctional nanoplatforms capable of generating localized hyperthermia or reactive oxygen species. These sophisticated nanotherapeutic approaches enhance selectivity and efficacy in CSC elimination, potentially circumventing drug resistance and cancer recurrence. Finally, we present an in-depth analysis of current challenges in translating nanomedicine-based CSC-targeted therapies from bench to bedside, offering critical insights into future research directions and clinical implementation. This review aims to provide a comprehensive framework for understanding the intersection of nanomedicine and CSC biology, contributing to more effective cancer treatment modalities.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 61-94"},"PeriodicalIF":18.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suprachoroidal space-inducing hydrogel-forming microneedles (SI-HFMN): An innovative platform for drug delivery to the posterior segment of the eye

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-03 DOI: 10.1016/j.bioactmat.2025.03.024

Jaibyung Choi , Suhyeon Shim , Jiwoo Shin , Ahhyun Lee , Jaan Strang , Tobias Braun , Reto Naef , Hyungil Jung

{"title":"Suprachoroidal space-inducing hydrogel-forming microneedles (SI-HFMN): An innovative platform for drug delivery to the posterior segment of the eye","authors":"Jaibyung Choi , Suhyeon Shim , Jiwoo Shin , Ahhyun Lee , Jaan Strang , Tobias Braun , Reto Naef , Hyungil Jung","doi":"10.1016/j.bioactmat.2025.03.024","DOIUrl":"10.1016/j.bioactmat.2025.03.024","url":null,"abstract":"<div><div>The suprachoroidal space (SCS), which exists between the sclera and choroid, offers a promising delivery route to the posterior segment of the eye (PSE) and is integrated with hollow microneedles (HMNs) for minimally invasive delivery. However, HMNs are limited by backflow owing to their narrow channel. Therefore, this study proposes a biocompatible SCS-inducing hydrogel-forming microneedle (SI-HFMN) with a specially designed candlelit shape that swells to separate the sclera from the choroid. The induced SCS provides a route for delivering loaded drugs to the PSE upon application. The optimized formulation of 20 % (w/w) poly(methyl vinyl ether-alt-maleic acid) (PMVE/MA) crosslinked with 7.5 % (w/w) polyethylene glycol (PEG) possesses sufficient mechanical strength (5.1 ± 0.7 N) to penetrate both the sclera and swell by 356 ± 28 %, to mechanically stimulate SCS formation. The formulation also recorded a drug absorption amount of 101 ± 9 μg/mg of hydrogel. Furthermore, <em>in vitro</em> and <em>ex vivo</em> experiments demonstrated the ability of the SI-HFMN to deliver drugs to the PSE via the formed SCS. Thus, this system offers an innovative method for drug delivery to PSE by inducing SCS formation.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 47-60"},"PeriodicalIF":18.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mytilus edulis foot protein mimics for tailoring long-acting endothelium-mimicking anti-thrombotic surfaces

IF 18 1区医学

Bioactive Materials Pub Date : 2025-04-01 DOI: 10.1016/j.bioactmat.2025.03.019

Zeyu Du , Yuting Huang , Qing Ma , Wentai Zhang , Yan Fu , Nan Huang , Xin Li , Zhilu Yang , Wenjie Tian

{"title":"Mytilus edulis foot protein mimics for tailoring long-acting endothelium-mimicking anti-thrombotic surfaces","authors":"Zeyu Du , Yuting Huang , Qing Ma , Wentai Zhang , Yan Fu , Nan Huang , Xin Li , Zhilu Yang , Wenjie Tian","doi":"10.1016/j.bioactmat.2025.03.019","DOIUrl":"10.1016/j.bioactmat.2025.03.019","url":null,"abstract":"<div><div>Surfaces with enduring and superior antithrombotic properties are essential for long-term blood-contacting devices. While current surface engineering strategies integrating anticoagulants and antiplatelet agents show promise in mimicking the non-thrombogenic properties of the endothelium, their long-term effectiveness remains limited. Here, we report an easy-to-perform, dual-biomimetic surface engineering strategy for tailoring long-acting endothelium-mimicking anti-thrombotic surfaces. We first designed a <em>Mytilus edulis</em> foot protein-5 (Mefp-5) mimic rich in amine and clickable alkynyl groups to polymerize-deposit a chemical robust coating onto the surface through a mussel-inspired adhesion mechanism. Then, a clickable nitric oxide (NO, an antiplatelet agent)-generating enzyme and the anticoagulant heparin were sequentially co-grafted onto the chemical robust coatings via click chemistry and carbodiimide chemistry. Our results demonstrate that this engineered surface achieved an impressive NO catalytic release efficiency of up to 88 %, while heparin retained 86 % of its bioactivity even after one month of exposure to PBS containing NO donor. Both <em>in vitro</em> and <em>in vivo</em> experiments confirmed that this robust endothelium-mimicking coating substantially reduces thrombosis formation. Overall, our long-acting endothelium-mimicking anti-thrombotic coatings present a promising and feasible strategy to address thrombosis-related challenges associated with blood-contacting devices.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"50 ","pages":"Pages 1-13"},"PeriodicalIF":18.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclodextrins as multifunctional tools for advanced biomaterials in tissue repair and regeneration

IF 18 1区医学

Bioactive Materials Pub Date : 2025-03-27 DOI: 10.1016/j.bioactmat.2025.03.018

Yu Bin Lee , Mi-Lang Kyun , Young Ju Lee , Hye-Eun Shim , Kang Moo Huh , Sun-Woong Kang

{"title":"Cyclodextrins as multifunctional tools for advanced biomaterials in tissue repair and regeneration","authors":"Yu Bin Lee , Mi-Lang Kyun , Young Ju Lee , Hye-Eun Shim , Kang Moo Huh , Sun-Woong Kang","doi":"10.1016/j.bioactmat.2025.03.018","DOIUrl":"10.1016/j.bioactmat.2025.03.018","url":null,"abstract":"<div><div>Cyclodextrins (CDs), characterized by their unique cyclic oligosaccharide structure and exceptional capacity for molecular encapsulation through host-guest interactions, have garnered significant attention as versatile building blocks in advanced biomaterials. This review explores the application of CD-based biomaterials in tissue engineering and regenerative medicine, emphasizing their synthesis, physicochemical characterization, biocompatibility, and translational potential. Special emphasis is placed on the development of CD-modified hydrogels, their interaction with host-guest molecules, and their use in advanced therapeutic strategies. Additionally, we discuss various fabrication methods for CD-based biomaterials, including physical and covalent crosslinking, self-assembly, and enzymatic crosslinking, each providing unique properties suited for advanced therapeutic strategies. Safety considerations and potential regulatory challenges associated with these materials are also examined. By providing a comprehensive overview of recent advancements, this review aims to highlight the promising role of cyclodextrins as multifunctional tools in enhancing tissue repair and regeneration, paving the way for innovative therapeutic solutions in clinical settings.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"49 ","pages":"Pages 627-651"},"PeriodicalIF":18.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0