Bioactive Materials最新文献_第10页

Single-cell RNA sequencing-guided engineering of mitochondrial therapies for intervertebral disc degeneration by regulating mtDNA/SPARC-STING signaling 单细胞RNA测序引导工程通过调节mtDNA/SPARC-STING信号治疗椎间盘退变的线粒体疗法

IF 18 1区医学

Bioactive Materials Pub Date : 2025-03-01 DOI: 10.1016/j.bioactmat.2025.02.036

Guoyu Yang , Chenpeng Dong , Zhaoxi Wu , Peng Wu , Cao Yang , Lanlan Li , Jianxiang Zhang , Xinghuo Wu

{"title":"Single-cell RNA sequencing-guided engineering of mitochondrial therapies for intervertebral disc degeneration by regulating mtDNA/SPARC-STING signaling","authors":"Guoyu Yang , Chenpeng Dong , Zhaoxi Wu , Peng Wu , Cao Yang , Lanlan Li , Jianxiang Zhang , Xinghuo Wu","doi":"10.1016/j.bioactmat.2025.02.036","DOIUrl":"10.1016/j.bioactmat.2025.02.036","url":null,"abstract":"<div><div>Intervertebral disc degeneration (IVDD) is a leading cause of discogenic low back pain, contributing significantly to global disability and economic burden. Current treatments provide only short-term pain relief without addressing the underlying pathogenesis. Herein we report engineering of biomimetic therapies for IVDD guided by single-cell RNA-sequencing data from human nucleus pulposus tissues, along with validation using animal models. In-depth analyses revealed the critical role of mitochondrial dysfunction in fibrotic phenotype polarization of nucleus pulposus cells (NPCs) during IVDD progression. Consequently, mitochondrial transplantation was proposed as a novel therapeutic strategy. Transplanted exogeneous mitochondria improved mitochondrial quality control in NPCs under pathological conditions, following endocytosis, separate distribution or fusion with endogenous mitochondria, and transfer to neighboring cells by tunneling nanotubes. Correspondingly, intradiscal mitochondrial transplantation significantly delayed puncture-induced IVDD progression in rats, demonstrating efficacy in maintaining mitochondrial homeostasis and alleviating pathological abnormalities. Furthermore, exogenous mitochondria were engineered with a bioactive, mitochondrial-targeting macromolecule to impart anti-oxidative and anti-inflammatory activities. The obtained multi-bioactive biotherapy exhibited significantly enhanced benefits in IVDD treatment, in terms of reversing IVDD progression and restoring structural integrity through the mtDNA/SPARC-STING signaling pathways. Overall, our engineered mitochondrial therapies hold great promise for treating IVDD and other musculoskeletal diseases linked to mitochondrial dysfunction.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 564-582"},"PeriodicalIF":18.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional magneto-electric and exosome-loaded hydrogel enhances neuronal differentiation and immunoregulation through remote non-invasive electrical stimulation for neurological recovery after spinal cord injury 多功能磁电和外泌体负载水凝胶通过远程无创电刺激促进脊髓损伤后神经系统恢复的神经元分化和免疫调节

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-28 DOI: 10.1016/j.bioactmat.2025.02.034

Wubo Liu , Qiang Liu , Zeqin Li , Chunjia Zhang , Zehui Li , Han Ke , Xin Xu , Xiaoxin Wang , Huayong Du , Zuliyaer Talifu , Yunzhu Pan , Xiaoxiong Wang , Jingyun Mao , Feng Gao , Degang Yang , Yan Yu , Xinyu Liu , Jianjun Li

{"title":"Multifunctional magneto-electric and exosome-loaded hydrogel enhances neuronal differentiation and immunoregulation through remote non-invasive electrical stimulation for neurological recovery after spinal cord injury","authors":"Wubo Liu , Qiang Liu , Zeqin Li , Chunjia Zhang , Zehui Li , Han Ke , Xin Xu , Xiaoxin Wang , Huayong Du , Zuliyaer Talifu , Yunzhu Pan , Xiaoxiong Wang , Jingyun Mao , Feng Gao , Degang Yang , Yan Yu , Xinyu Liu , Jianjun Li","doi":"10.1016/j.bioactmat.2025.02.034","DOIUrl":"10.1016/j.bioactmat.2025.02.034","url":null,"abstract":"<div><div>Intervention in the differentiation of neural stem cells (NSCs) is emerging as a highly promising approach for the treatment of spinal cord injury (SCI). However, NSCs at the injury site often suffer from low survival and uncontrolled differentiation. Whereas electrical stimulation has proven effective in regulating the fate of NSCs and promoting tissue repair, however, conventional electrical stimulation therapy has failed to be widely applied due to challenges such as invasiveness and technical complexity. To overcome these limitations, we developed a biomimetic magneto-electric hydrogel incorporating Fe<sub>3</sub>O<sub>4</sub>@BaTiO<sub>3</sub> core-shell nanoparticles and human umbilical mesenchymal stem cell exosomes (HUMSC-Exos) around the concept of constructing remote noninvasive electrical stimulation for the synergistic treatment of SCI. The Fe<sub>3</sub>O<sub>4</sub>@BaTiO<sub>3</sub> is activated by the peripheral magnetic field to generate electrical stimulation, which, in conjunction with the synergistic effects of HUMSC-Exos, significantly alleviates the early inflammatory response associated with SCI and enhances the regeneration of newborn neurons and axons, thereby creating favorable conditions for functional recovery post-SCI. Our findings indicate that applying this magneto-exosome hydrogel in a rat model of SCI leads to substantial functional recovery. This innovative combination represents a promising therapeutic strategy for SCI repair.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 510-528"},"PeriodicalIF":18.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special issue: Recent advances in immunotherapy and immunoengineering 特刊：免疫治疗和免疫工程的最新进展

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-28 DOI: 10.1016/j.bioactmat.2025.01.038

Gloria B. Kim , Quanyin Hu , Philipp C. Rommel

引用次数: 0

Innovative liquid embolic agents based on deep eutectic solvent: Rapid gelation in situ via solvent exchange with water for endovascular embolization 基于深度共熔溶剂的创新液体栓塞剂：通过溶剂与水交换，在原位快速凝胶化，用于血管内栓塞

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-28 DOI: 10.1016/j.bioactmat.2025.02.037

Yitong Zhou , Menghui Liu , Chuandong He , Jiayuan Lin , Yanlv Chen , Mingyu Yu , Yuhan Jiang , Xin Peng

{"title":"Innovative liquid embolic agents based on deep eutectic solvent: Rapid gelation in situ via solvent exchange with water for endovascular embolization","authors":"Yitong Zhou , Menghui Liu , Chuandong He , Jiayuan Lin , Yanlv Chen , Mingyu Yu , Yuhan Jiang , Xin Peng","doi":"10.1016/j.bioactmat.2025.02.037","DOIUrl":"10.1016/j.bioactmat.2025.02.037","url":null,"abstract":"<div><div>Current liquid embolic agents face several challenges, including poor biocompatibility and vascular recanalization. Herein, we propose an innovative liquid embolic agent composed of a coenzyme-based polymer (poly lipoic acid, PLA) and a biocompatible solvent (deep eutectic solvent, DES). The agent undergoes phase transformation to form a stable hydrogel in situ through solvent exchange with water, thereby enabling safe and effective embolization. First, DES is obtained by heating a mixture of choline chloride (ChCl) and glycerol (Gly). Subsequently, lipoic acid (LA) is incorporated into the DES and heated to produce the PLA/DES complex. Owing to the strong hydrogen bonding between the DES and PLA, the DES acts as a solvent while also inhibiting PLA depolymerization. Upon contact with blood, most of the DES exchange with water, whereas some amount of ChCl integrates within the PLA via strong hydrogen bonding. This hydrogen bonding not only prevents PLA depolymerization but also reinforces the PLA network, resulting in a stable PLA hydrogel rather than depolymerized LA monomers. Furthermore, liquid-metal (LM) nanoparticles are incorporated to fabricate radiopaque PLA/LM/DES. PLA/LM/DES shows better in vitro hemocompatibility and cytocompatibility, milder inflammatory response in a rat model, and more effective and safer embolization in a rabbit model than a commercial embolic agent (Onyx). Thus, this work provides an innovative liquid embolic agent and broadens the biomedical applications of DES.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 550-563"},"PeriodicalIF":18.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced biomaterials for targeting mature biofilms in periodontitis therapy 成熟生物膜靶向治疗牙周炎的先进生物材料

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-27 DOI: 10.1016/j.bioactmat.2025.02.026

Jiawen Tao , Yirong Sun , Guoliang Wang , Jingru Sun , Shujun Dong , Jianxun Ding

{"title":"Advanced biomaterials for targeting mature biofilms in periodontitis therapy","authors":"Jiawen Tao , Yirong Sun , Guoliang Wang , Jingru Sun , Shujun Dong , Jianxun Ding","doi":"10.1016/j.bioactmat.2025.02.026","DOIUrl":"10.1016/j.bioactmat.2025.02.026","url":null,"abstract":"<div><div>Periodontitis is a chronic inflammatory disease primarily caused by bacteria, leading to inflamed and bleeding gums, periodontal pocket formation, and bone loss. Affecting 70%–90% of adults over 65, periodontitis is a leading cause of tooth loss and significantly impacts quality of life. Standard treatments, including subgingival scraping and antibiotics, have limitations, and antibiotic resistance among periodontal pathogens is an increasing concern. Biofilms are barriers to drugs and immune responses, contributing to bacterial resistance and reducing antibiotic effectiveness. Due to their adjustable physicochemical properties, bioactive materials potentially eliminate bacterial biofilms, presenting a promising alternative for periodontitis therapy. In this review, the recent innovations in biomaterials for removing mature biofilms in periodontitis are examined, and their broader potential is discussed. Additionally, the compositions of bacterial biofilms, formation pathways, and intrinsic drug resistance mechanisms are discussed. Finally, the strategies for optimizing subgingival biofilm removal in periodontitis are highlighted, such as targeting biofilms-embedded bacteria, disrupting the extracellular polymeric substances, and utilizing combined approaches. A comprehensive understanding of the properties of biomaterials guides the rational design of highly targeted and effective therapies for periodontitis.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 474-492"},"PeriodicalIF":18.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metastructure and strain-defect engineered Cu-doped TiOx coating to enhance antibacterial sonodynamic therapy 元结构和应变缺陷工程cu掺杂TiOx涂层增强抗菌声动力治疗

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-27 DOI: 10.1016/j.bioactmat.2025.02.028

Songsong Wang , Ji Tan , Haifeng Zhang , Shiwei Guan , Yibo Zeng , Xiaoshuang Nie , Hongqin Zhu , Shi Qian , Xuanyong Liu

{"title":"Metastructure and strain-defect engineered Cu-doped TiOx coating to enhance antibacterial sonodynamic therapy","authors":"Songsong Wang , Ji Tan , Haifeng Zhang , Shiwei Guan , Yibo Zeng , Xiaoshuang Nie , Hongqin Zhu , Shi Qian , Xuanyong Liu","doi":"10.1016/j.bioactmat.2025.02.028","DOIUrl":"10.1016/j.bioactmat.2025.02.028","url":null,"abstract":"<div><div>Sonodynamic therapy (SDT) has attracted widespread attention in treatment of implant-associated infections, one of the key factors leading to implant failure. Nevertheless, constructing efficient ultrasound-triggered coatings on implant surfaces remains a challenge. Herein, an acoustic metastructure Cu-doped defective titanium oxide coating (Cu-TiO<sub><em>x</em></sub>) with lattice strain was constructed <em>in situ</em> on titanium implant to realize effective sonocatalysis. The redistribution of Cu atoms broke the pristine lattice of TiO<sub>2</sub> during the thermal reduction treatment to regulate its energy structure, which favored separation of electron-hole pairs generated by ultrasound radiation to enhance the sonocatalytic generation of reactive oxygen species. In addition, the acoustic metastructure enhanced the absorption of ultrasound by Cu-TiO<sub><em>x</em></sub> metastructure coating, which further promoted its sonocatalytic effect. Thus, Cu-TiO<sub><em>x</em></sub> metastructure coating could efficiently eliminate <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> infections under ultrasonic irradiation in 10 min. Besides, the osteogenic property of implant was significantly improved after infection clearance <em>in vivo</em>. This work provides a fresh perspective on the design of SDT biosurfaces based on metastructure and strain-defect engineering.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 458-473"},"PeriodicalIF":18.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A micro-metabolic rewiring assay for assessing hypoxia-associated cancer metabolic heterogeneity 用于评估缺氧相关癌症代谢异质性的微代谢重组试验

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-27 DOI: 10.1016/j.bioactmat.2025.02.030

Jeong Min Oh , Tianze Guo , Hydari Masuma Begum , Saci-Elodie Marty , Liang Sha , Cem Kilic , Hao Zhou , Yali Dou , Keyue Shen

{"title":"A micro-metabolic rewiring assay for assessing hypoxia-associated cancer metabolic heterogeneity","authors":"Jeong Min Oh , Tianze Guo , Hydari Masuma Begum , Saci-Elodie Marty , Liang Sha , Cem Kilic , Hao Zhou , Yali Dou , Keyue Shen","doi":"10.1016/j.bioactmat.2025.02.030","DOIUrl":"10.1016/j.bioactmat.2025.02.030","url":null,"abstract":"<div><div>Cancer metabolism plays an essential role in therapeutic resistance, where significant inter- and intra-tumoral heterogeneity exists. Hypoxia is a prominent driver of metabolic rewiring behaviors and drug responses. Recapitulating the hypoxic landscape in the tumor microenvironment thus offers unique insights into heterogeneity in metabolic rewiring and therapeutic responses, to inform better treatment strategies. There remains a lack of scalable tools that can readily interface with imaging platforms and resolve the heterogeneous behaviors in hypoxia-associated metabolic rewiring. Here we present a micro-metabolic rewiring (μMeRe) assay that provides the scalability and resolution needed to characterize the metabolic rewiring behaviors of different cancer cells in the context of hypoxic solid tumors. Our assay generates hypoxia through cellular metabolism without external gas controls, enabling the characterization of cell-specific intrinsic ability to drive hypoxia and undergo metabolic rewiring. We further developed quantitative metrics that measure the metabolic plasticity through phenotypes and gene expression. As a proof-of-concept, we evaluated the efficacy of a metabolism-targeting strategy in mitigating hypoxia- and metabolic rewiring-induced chemotherapeutic resistance. Our study and the scalable platform thus lay the foundation for designing more effective cancer treatments tailored toward specific metabolic rewiring behaviors.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 493-509"},"PeriodicalIF":18.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microenvironment-responsive coating for vascular stents to regulate coagulation-inflammation interaction and promote vascular recovery 血管支架微环境响应涂层调节凝-炎症相互作用，促进血管恢复

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-26 DOI: 10.1016/j.bioactmat.2025.02.031

Qiongjun Zhu , Zhezhe Chen , Dan'an Wang , Xiaolu Jiao , Yi Luan , Min Wang , Rifang Luo , Yunbing Wang , Guosheng Fu , Yanan Wang , Wenbin Zhang

{"title":"Microenvironment-responsive coating for vascular stents to regulate coagulation-inflammation interaction and promote vascular recovery","authors":"Qiongjun Zhu , Zhezhe Chen , Dan'an Wang , Xiaolu Jiao , Yi Luan , Min Wang , Rifang Luo , Yunbing Wang , Guosheng Fu , Yanan Wang , Wenbin Zhang","doi":"10.1016/j.bioactmat.2025.02.031","DOIUrl":"10.1016/j.bioactmat.2025.02.031","url":null,"abstract":"<div><div>Early coagulation-inflammation interaction and late in-stent restenosis undermine the efficacy of vascular stents after implantation. Targeting the interplay between inflammation and coagulation, and smooth muscle cell (SMC) proliferation, we presented a microenvironment-responsive coating designed to regulate tissue responses and vascular regeneration throughout the remodeling process. Coagulation was inhibited by incorporating anticoagulant tirofiban into the coating. MMP9-responsive nanoparticles embedded in the coating released salvianolic acid A to modulate inflammatory cell behavior and inhibit SMC dysfunction. By effectively interfering with clotting and inflammation, the coating suppressed platelet-fibrin interaction and formation of platelet-monocyte aggregates, thereby mitigating adverse effects on reendothelialization. Its ability to influence SMC proliferation and migration resulted in reduced intimal hyperplasia. Coated stents were shown to significantly regulate tissue regeneration, improve the vascular environment and even reduced the lipid content in the narrowed atherosclerotic vessels <em>in viv</em>o. This direct approach enhanced the vascular tissue regeneration after stent implantation, and offered promising insights for optimizing vascular stent design.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 443-457"},"PeriodicalIF":18.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Living joint prosthesis with in-situ tissue engineering for real-time and long-term osteoarticular reconstruction 基于原位组织工程的活体关节假体用于实时和长期的骨关节重建

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-26 DOI: 10.1016/j.bioactmat.2025.01.036

Wei Sun , Hongwei Wu , Yiyang Yan , Xianzhu Zhang , Xudong Yao , Rui Li , Jingyi Zuo , Wenyue Li , Hongwei Ouyang

{"title":"Living joint prosthesis with in-situ tissue engineering for real-time and long-term osteoarticular reconstruction","authors":"Wei Sun , Hongwei Wu , Yiyang Yan , Xianzhu Zhang , Xudong Yao , Rui Li , Jingyi Zuo , Wenyue Li , Hongwei Ouyang","doi":"10.1016/j.bioactmat.2025.01.036","DOIUrl":"10.1016/j.bioactmat.2025.01.036","url":null,"abstract":"<div><div>The reconstruction of large osteoarticular defects caused by tumor resection or severe trauma remains a clinical challenge. Current metal prostheses exhibit a lack of osteo-chondrogenic functionality and demonstrate poor integration with host tissues. This often results in complications such as abnormal bone absorption and prosthetic loosening, which may necessitate secondary revisions. Here, we propose a paradigm-shifting “living prosthesis” strategy that combines a customized 3D-printed hollow titanium humeral prosthesis with engineered bone marrow condensations presenting bone morphogenetic protein-2 (BMP-2) and transforming growth factor–β3 (TGF-β3) from encapsulated silk fibroin hydrogels. This innovative approach promotes <em>in situ</em> endochondral defect regeneration of the entire humeral head while simultaneously providing immediate mechanical support. In a rabbit model of total humerus resection, the designed “living prosthesis” achieved weight, macroscopic and microscopic morphologies that were comparable to those of undamaged native joints at 2 months post-implantation, with organized osteochondral tissues were regenerated both around and within the prosthesis. Notably, the “living prosthesis” displayed significantly higher osteo-integration than the blank metal prosthesis did, as evidenced by a 3-fold increase in bone ingrowth and a 2-fold increase in mechanical pull-out strength. Furthermore, the \"living prosthesis\" restored joint cartilage function, with rabbits exhibiting normal gait and weight-bearing capacity. The successful regeneration of fully functional humeral head tissue from a single implanted prosthesis represents technical advance in designing bioactive bone prosthesis, with promising implications for treating extreme-large osteochondral defects.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 431-442"},"PeriodicalIF":18.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ UNIversal Orthogonal Network (UNION) bioink deposition for direct delivery of corneal stromal stem cells to corneal wounds 原位通用正交网络（UNION）生物链接沉积用于直接递送角膜基质干细胞到角膜创面

IF 18 1区医学

Bioactive Materials Pub Date : 2025-02-24 DOI: 10.1016/j.bioactmat.2025.02.009

Lucia G. Brunel , Betty Cai , Sarah M. Hull , Uiyoung Han , Thitima Wungcharoen , Gabriella Maria Fernandes-Cunha , Youngyoon Amy Seo , Patrik K. Johansson , Sarah C. Heilshorn , David Myung

{"title":"In situ UNIversal Orthogonal Network (UNION) bioink deposition for direct delivery of corneal stromal stem cells to corneal wounds","authors":"Lucia G. Brunel , Betty Cai , Sarah M. Hull , Uiyoung Han , Thitima Wungcharoen , Gabriella Maria Fernandes-Cunha , Youngyoon Amy Seo , Patrik K. Johansson , Sarah C. Heilshorn , David Myung","doi":"10.1016/j.bioactmat.2025.02.009","DOIUrl":"10.1016/j.bioactmat.2025.02.009","url":null,"abstract":"<div><div>The scarcity of human donor corneal graft tissue worldwide available for corneal transplantation necessitates the development of alternative therapeutic strategies for treating patients with corneal blindness. Corneal stromal stem cells (CSSCs) have the potential to address this global shortage by allowing a single donor cornea to treat multiple patients. To directly deliver CSSCs to corneal defects within an engineered biomatrix, we developed a UNIversal Orthogonal Network (UNION) collagen bioink that crosslinks <em>in situ</em> with a bioorthogonal, covalent chemistry. This cell-gel therapy is optically transparent, stable against contraction forces exerted by CSSCs, and permissive to the efficient growth of corneal epithelial cells. Furthermore, CSSCs remain viable within the UNION collagen gel precursor solution under standard storage and transportation conditions. This approach promoted corneal transparency and re-epithelialization in a rabbit anterior lamellar keratoplasty model, indicating that the UNION collagen bioink serves effectively as an <em>in situ</em>-forming, suture-free therapy for delivering CSSCs to corneal wounds.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"48 ","pages":"Pages 414-430"},"PeriodicalIF":18.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0