Antioxidants最新文献

{"title":"Impact of Maternal High-Fat Diet on Offspring Cardiovascular-Kidney-Metabolic Health: Spotlight on Oxidative Stress.","authors":"Chien-Ning Hsu, Chih-Kuang Chen, Chih-Yao Hou, Yu-Wei Chen, Guo-Ping Chang-Chien, Shu-Fen Lin, You-Lin Tain","doi":"10.3390/antiox14091136","DOIUrl":"10.3390/antiox14091136","url":null,"abstract":"<p><p>Cardiovascular-kidney-metabolic syndrome (CKMS) encompasses interconnected cardiovascular, renal, and metabolic disorders, including obesity, hypertension, and type 2 diabetes. Oxidative stress is increasingly recognized as a central driver of this multi-organ dysfunction. Among maternal influences, exposure to a high-fat diet (HFD) during pregnancy and lactation consistently predisposes offspring to CKMS-related phenotypes in animal models. While oxidative stress is implicated as a key mediator, its precise role in developmental programming remains unclear, and comparing the differences in its role between overt CKMS and CKM programming is critical. Critical gaps include whether oxidative stress acts uniformly or in an organ- and time-specific manner, which signals initiate long-term redox alterations, and whether these effects are reversible. Furthermore, its interactions with other programming pathways-such as renin-angiotensin system activation, epigenetic dysregulation, gut microbiota imbalance, and altered nutrient sensing-remain insufficiently explored. This review uniquely highlights maternal HFD-induced oxidative stress as a mechanistic axis of CKMS programming and delineates unresolved questions that limit translation. By integrating evidence across organ systems and proposing priorities for multi-organ profiling, refined models, and longitudinal human studies, we outline a forward-looking agenda for the field. Ultimately, clarifying how maternal HFD and oxidative stress shape offspring CKMS risk is essential to inform targeted antioxidant strategies to reduce the intergenerational transmission of CKMS risk.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Dual Role of NOX4 in Cardiovascular Diseases: Driver of Oxidative Stress and Mediator of Adaptive Remodeling.","authors":"Pauline Labbé, Eric Thorin, Nathalie Thorin-Trescases","doi":"10.3390/antiox14091137","DOIUrl":"10.3390/antiox14091137","url":null,"abstract":"<p><p>NADPH oxidase 4 (NOX4) plays a crucial role in regulating cardiac function and pathology through its involvement in oxidative stress, fibrosis, and maladaptive remodeling. Studies have demonstrated that NOX4 is upregulated in response to various cardiovascular stressors, including heart failure, myocardial infarction, arrhythmias, and diabetes. This upregulation contributes to detrimental processes like fibrosis, hypertrophy, and inflammation, which are hallmarks of cardiovascular diseases. Inhibition or knockout of NOX4 has shown promise in mitigating these pathological changes, suggesting that NOX4 represents a potential therapeutic target for treating heart disease. However, NOX4's role is not entirely negative. It also plays a protective role in the heart, supporting myocardial remodeling and angiogenesis and regulating cardiac energy metabolism. Its constitutive ROS production and ability to respond to environmental cues like hypoxia help maintain cellular homeostasis and facilitate adaptive responses to stress. The impact of NOX4 on cardiac health depends not only on its expression level but also on the nature of the stress, the duration of activation, and the balance between protective signaling and oxidative injury. Collectively, the findings suggest that NOX4 functions as a redox sensor, modulating cellular responses to fluctuations in oxidative stress by signaling the need to re-establish redox homeostasis. The ultimate impact of cardiac NOX4 activity, whether protective or deleterious, is highly context-dependent and should not be evaluated through a singular interpretative framework. In conclusion, NOX4 is a dual-function enzyme that can both exacerbate and protect against cardiac pathology, making it a promising, though complex, therapeutic target for various cardiovascular diseases.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen Gas Mitigates Acute Hypoxia-Induced Oxidative and Inflammatory Brain Injuries in Medaka (<i>Oryzias latipes</i>).","authors":"Eriko Sato, Naohiro Shimamura, Chikako Saiki, Katsuhisa Sunada, Nobuhiko Miwa, Li Xiao","doi":"10.3390/antiox14091130","DOIUrl":"10.3390/antiox14091130","url":null,"abstract":"<p><p>Hypoxia-induced oxidative stress and inflammation in the brain are critical contributors to neurological disorders. Hydrogen gas has emerged as a therapeutic agent with potent antioxidant and anti-inflammatory properties. In this study, we evaluated the protective effects of hydrogen against acute hypoxia-induced brain injuries in medaka. Fish were exposed to hypoxia and then recovered in water bubbled with air, hydrogen, or ozone. LOX-1 hypoxia probe imaging and HIF-1α immunostaining showed persistent tissue hypoxia in the air and ozone groups, which was significantly reduced by hydrogen treatment. Histological analysis revealed extensive vascular congestion in the midbrain after hypoxia, which was markedly alleviated by hydrogen. TUNEL assay demonstrated that hydrogen suppressed hypoxia-induced neuronal apoptosis. Immunohistochemistry and ELISA showed elevated levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and proinflammatory markers (COX-2, IL-6, TNF-α) in the brains of air- and ozone-treated fish; these increases were significantly attenuated by hydrogen. ORAC assay confirmed that hydrogen restored brain antioxidant capacity. Behavioral analysis further demonstrated that hydrogen treatment improved locomotor activity and stabilized respiratory function. These results indicate that hydrogen protects medaka against hypoxia-induced oxidative and inflammatory injuries and may represent a promising therapeutic strategy for hypoxia-related neurological disorders.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovary Metal Toxicity Remediation by Agro-Food Waste: Evidence for a Regulatory Mechanism of Oxidative Stress by Banana (<i>Musa cavendish</i>) Peel Extract.","authors":"Boma F Eddie-Amadi, Rubina Vangone, Valeria Guerretti, Harrison A Ozoani, Kenneth O Okolo, Dokubo Awolayeofori, Tamuno-Boma Odinga-Israel, Kpobari W Nkpaa, Emidio M Sivieri, Orish E Orisakwe, Giulia Guerriero","doi":"10.3390/antiox14091129","DOIUrl":"10.3390/antiox14091129","url":null,"abstract":"<p><p>Banana (<i>Musa cavendish</i>) peel, usually discarded as waste, is a polyphenol-rich source with antioxidant and chelating properties. This study evaluated its ability to mitigate ovarian toxicity induced by a heavy metal mixture (HMM) consisting of Hg, Mn, Pb, and Al in female rats. Animals received the HMM with or without banana peel extract at 200, 400, and 800 mg/kg dosages for 60 days. Co-treatment dose-dependently reduced ovarian metal accumulation, attenuated oxidative and nitrosative stress (MDA, NO), restored antioxidant enzyme activities (SOD, CAT, GSH, GPx), and modulated pro-inflammatory (IL-6, TNF-α), apoptotic (Caspase-3), and transcriptional factors (NF-κB, Nrf2). The gonadal endocrine profile also improved gonadotropins (FSH, LH), prolactin (PRL), and progesterone (P), which were normalized at the medium dose (400 mg/kg), and demonstrated a clear dose-related effect. Histological examination further revealed that this dose most effectively improved ovarian tissue. GC-MS analysis identified bioactive compounds including resveratrol, proanthocyanidins, and anthocyanidins, supporting both antioxidant and chelating actions. These findings demonstrate that banana peel extract exerts a dual, dose-dependent protective role in the gonad, limiting metal burden while enhancing redox defenses, and highlight its translational potential as a sustainable agro-food waste product in reproductive toxicology.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications of Antioxidant Nanoparticles in Immune-Mediated Inflammatory Diseases.","authors":"Hong-Wei Shi, Bo-Cheng Yang, Yun-Qing Ren, Yi Xue","doi":"10.3390/antiox14091128","DOIUrl":"10.3390/antiox14091128","url":null,"abstract":"<p><p>Immune-mediated inflammatory diseases (IMIDs) encompass a wide range of disorders, including autoimmune, acute, and chronic inflammatory conditions, which are often characterized by immune dysregulation and excessive oxidative stress. Oxidative stress plays a pivotal role in the initiation and progression of these diseases by promoting tissue damage and sustaining inflammation. However, conventional antioxidant therapies are limited by poor bioavailability, inadequate targeting, and short-lived efficacy. In recent years, nano-antioxidants have emerged as a promising therapeutic approach due to their enhanced stability, targeted delivery capabilities, and multifunctional therapeutic effects. This review provides a comprehensive overview of recent advances in the application of nano-antioxidants in the treatment of IMIDs. Their therapeutic roles are categorized into three major groups: autoimmune diseases, acute inflammatory diseases, and chronic inflammatory diseases. In autoimmune disorders such as alopecia areata and multiple sclerosis, nano-antioxidants have demonstrated the ability to reduce oxidative damage, modulate immune responses, and alleviate clinical symptoms. In acute inflammatory conditions, including acute kidney injury and acute liver injury, these nanomaterials exert protective effects by scavenging ROS, mitigating tissue injury, and restoring organ function. In chronic inflammatory diseases such as inflammatory bowel disease and ulcerative colitis, nano-antioxidants contribute to maintaining mucosal integrity, suppressing chronic inflammation, and improving therapeutic outcomes through localized delivery and sustained release. In summary, nano-antioxidants represent a novel and promising therapeutic strategy for the management of IMIDs. Their unique physicochemical properties offer significant advantages over traditional treatments. Further research is needed to optimize their delivery platforms, evaluate long-term safety, and facilitate clinical translation.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging and Versatile Non-Mammalian Model Organisms for Studying the In Vivo Antioxidant Properties of Food-Derived Bioactive Compounds.","authors":"Alejandra Miranda-Carrazco, Verenice Torres-Salas, Rosy G Cruz-Monterrosa, Monzerrat Rosas-Espejel, Ildefonso Guerrero-Encinas, Javier N González-González, Luis Quihui-Cota, Andrea M Liceaga, José E Aguilar-Toalá","doi":"10.3390/antiox14091127","DOIUrl":"10.3390/antiox14091127","url":null,"abstract":"<p><p>In recent years, there has been increased attention to exploring non-mammalian model organisms to study the antioxidant properties of bioactive compounds. These models include both unicellular organisms, such as <i>Escherichia coli</i> and <i>Saccharomyces cerevisiae,</i> and multicellular organisms, such as <i>Caenorhabditis elegans</i>, <i>Drosophila melanogaster</i>, and <i>Danio rerio</i>. In particular, multicellular models have emerged as promising systems due to their ease of establishing systems and maintenance, short duration of experiments, ease of genetic manipulation and genome-wide screening, availability as off-the-shelf models, safety, and cost-effectiveness. Notably, these organisms share a high degree of gene homology with humans, ranging from 65% to 84%, which positions them as powerful platforms for investigating human disease mechanisms. These advantages make them attractive candidates for investigating the potential health benefits of various bioactive compounds before resorting to mammalian models. This review delves into the rationale for utilizing these emerging non-mammalian model organisms during preliminary stages of research, emphasizing their distinct advantages over traditional mammalian models. It also highlights their significant contributions to advancing our understanding of the antioxidant mechanisms of bioactive compounds, shedding light on their potential therapeutic implications for human health. By leveraging these models, researchers can efficiently screen and validate bioactive compounds, laying a robust foundation for subsequent translational studies in mammalian systems.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Superoxide Dismutase Gene Family in Chili Pepper (<i>Capsicum annuum</i> L.): Molecular Characterization and Involvement in Redox Regulation Under Chilling Stress.","authors":"Seo Hyeon Ban, Chae Eun Song, Seung Hee Eom, Tae Kyung Hyun","doi":"10.3390/antiox14091131","DOIUrl":"10.3390/antiox14091131","url":null,"abstract":"<p><p>Chilling stress is a major abiotic factor that limits chili pepper (<i>Capsicum annuum</i> L.) cultivation by disrupting redox homeostasis, thereby impairing growth and fruit productivity. Superoxide dismutases (SODs), which catalyze the conversion of superoxide radicals into hydrogen peroxide and oxygen, serve as key components of the plant antioxidant defense system. However, the SOD gene family in chili pepper has not been comprehensively characterized. Therefore, this study aimed to characterize the SOD gene family in chili pepper and investigate their responses to chilling stress. We identified nine putative <i>CaSOD</i> genes and classified them into CZSOD, FeSOD, and MnSOD clades based on phylogenetic relationships and conserved domain architecture. Bioinformatic analyses revealed variation in physicochemical properties and predicted subcellular localizations, suggesting functional diversification. Transcriptome profiling indicated tissue-specific expression, with several <i>CaSODs</i> preferentially expressed in fruits and floral buds, while qRT-PCR analysis demonstrated that six <i>CaSODs</i> were transcriptionally induced under chilling stress. Functional validation in <i>Nicotiana benthamiana</i> leaves showed that transient expression of four selected <i>CaSODs</i> significantly enhanced SOD activity in an isoform-specific manner. Future studies should validate these genes across diverse chili pepper cultivars under field conditions and assess their potential for integration into breeding programs. Collectively, these findings provide new insights into the molecular and functional diversity of CaSODs, highlight their role in maintaining redox balance under chilling stress, and provide useful genetic resources for breeding stress-tolerant chili pepper and related crops.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time to Reset: The Interplay Between Circadian Rhythms and Redox Homeostasis in Skeletal Muscle Ageing and Systemic Health.","authors":"Elizabeth Sutton, Vanja Pekovic-Vaughan","doi":"10.3390/antiox14091132","DOIUrl":"10.3390/antiox14091132","url":null,"abstract":"<p><p>Skeletal muscle plays vital roles in locomotion, metabolic regulation and endocrine signalling. Critically, it undergoes structural and functional decline with age, leading to a progressive loss of muscle mass and strength (sarcopenia) and contributing to a systemic loss of tissue resilience to stressors of multiple tissue systems (frailty). Emerging evidence implicates misalignments in both the circadian molecular clock and redox homeostasis as major drivers of age-related skeletal muscle deterioration. The circadian molecular clock, through core clock components such as BMAL1 and CLOCK, orchestrates rhythmic gene, protein and myokine expression impacting diurnal regulation of skeletal muscle structure and metabolism, mitochondrial function, antioxidant defence, extracellular matrix organisation and systemic inter-tissue communication. In parallel, the master redox regulator, NRF2, maintains cellular antioxidant defence, tissue stress resistance and mitochondrial health. Disruption of either system impairs skeletal muscle contractility, metabolism, and regenerative capacity as well as systemic homeostasis. Notably, NRF2-mediated redox signalling is clock-regulated and, in turn, affects circadian clock regulation. Both systems are responsive to external cues such as exercise and hormones, yet studies do not consistently include circadian timing or biological sex as key methodological variables. Given that circadian regulation shifts with age and differs between sexes, aligning exercise interventions with one's own chronotype may enhance health benefits, reduce adverse side effects, and overcome anabolic resistance with ageing. This review highlights the essential interplay between circadian and redox systems in skeletal muscle homeostasis and systemic health and argues for incorporating personalised chrono-redox approaches and sex-specific considerations into future experimental research and clinical studies, aiming to improve functional outcomes in age-related sarcopenia and broader age-related metabolic and musculoskeletal conditions.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recovery of SIRT3-SOD2 Axis and Mitophagy by Short-Term Calorie Restriction in Old Rat Soleus Skeletal Muscle.","authors":"Rosa Di Lorenzo, Anna Picca, Guglielmina Chimienti, Christiaan Leeuwenburgh, Vito Pesce, Angela Maria Serena Lezza","doi":"10.3390/antiox14091125","DOIUrl":"10.3390/antiox14091125","url":null,"abstract":"<p><p>Age-related mitochondrial dysfunction is involved in the progressive loss of mass and strength of skeletal muscle with aging. The effects of a short-term calorie restriction (ST-CR) were assessed in the oxidative skeletal soleus muscle (Sol) from 27-month-old rats and compared with those of a CR in combination with resveratrol (RSV) (ST-CR + RSV). PGC-1α and PRXIII proteins showed a marked decrease in both ST-CR and ST-CR + RSV rats. The SIRT3 protein presented a very relevant increase in both ST groups. ST-CR and ST-CR + RSV elicited a marked increase in SOD2 protein amount and activity. ST-CR and ST-CR + RSV led to recovery of the SIRT3-SOD2 axis as a fast/early response. ST-CR and ST-CR + RSV did not affect the MFN2 protein, whereas both treatments induced a relevant increase in DRP1 protein. ST-CR and ST-CR + RSV induced a decrease in Parkin protein, suggestive of rescued mitophagy, leading to the elimination of dysfunctional mitochondria. Such a response likely enhanced the fission-mediated elimination of mitochondria, supported by the marked increase in DRP1. MtDNA copy number and TFAM protein were not changed by any ST treatment. The mtDNA oxidative damage level was strongly increased by both ST treatments. All the effects elicited by ST-CR and ST-CR + RSV were specific to the oxidative type fibers.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466821/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Addition of Marigold Extract to the Diet Improved the Performance of Laying Hens in the Late Laying Period by Increasing Their Antioxidant Capacity, Lipid Metabolism, and Microbial Composition.","authors":"Qiyue Yang, Keying Zhang, Jianping Wang, Shiping Bai, Qiufeng Zeng, Huanwei Peng, Yadong Mu, Yue Xuan, Shanshan Li, Xuemei Ding","doi":"10.3390/antiox14091126","DOIUrl":"10.3390/antiox14091126","url":null,"abstract":"<p><p>The decrease in the production performance of laying hens during the later laying stage can be attributed to multiple factors, chief among them being oxidative stress and disrupted lipid metabolism. Quercetagetin, the active component of marigold extract, is a flavonoid whose polyhydroxy structure has greater antioxidant capacity than other flavonoids. In this study, we determined whether adding marigold extract to the diet can improve the antioxidant capacity and lipid metabolism of late-laying hens to increase their performance. In total, 800 <i>Lohmann</i> laying hens (45 weeks old) were randomly assigned to five treatment groups, each consisting of eight replicates (20 hens per replicate). Throughout the experiment, which lasted 24 weeks, the hens were fed diets containing 0, 200, 400, 600, or 800 mg/kg marigold extract. The results of the study showed that the addition of marigold extract to the feed significantly increased the egg production rate and qualified egg rate and reduced the feed-to-egg ratio; it also reduced the levels of oxidized products in eggs, serum, and liver, and improved the antioxidant capacity of the organism. Mechanistic studies showed that marigold extract could activate the <i>Keap</i>1-<i>Nrf</i>2 signaling pathway and up-regulate the gene expression of <i>CAT</i>, <i>SOD</i>, <i>GPX</i>, and <i>Nrf</i>2. In addition, marigold extract increased HDL and VLDL content in the liver, decreased TC and LDL content, and alteration of the cecal microbial composition. In conclusion, marigold extract showed good application value and potential as a safe and effective antioxidant additive in the late laying stage of laying hens.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 9","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}