Inactivated Cells and Metabolites of Saccharomyces boulardii Alleviate Inflammation Damage in Caco-2 Monolayer Cells and Mice with Ulcerative Colitis.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yuxin Jin, Zehui Niu, Menglin Feng, Huilian Che, Zhihong Liang
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引用次数: 0

Abstract

Saccharomyces boulardii (S. boulardii) has attracted widespread attention due to its antimicrobial and anti-inflammatory properties. In this study, we prepared postbiotics from the heat-inactivated cells (HIC) and cell-free supernatant (CFS) of S. boulardii, with the important component L-arginine (Arg) from the metabolic products included as one of the experimental groups. The results showed that in LPS-stimulated Caco-2 cells, HIC, CFS, and Arg protect intestinal epithelial barrier integrity by inhibiting the expression of TNF-α, IL-1β, and IL-6 while enhancing the expression of occludin and ZO-1 proteins. In dextran sulfate sodium (DSS)-induced colitis mice, HIC, CFS, and Arg alleviate symptoms such as weight loss and colonic damage while suppressing the upregulation of pro-inflammatory factors and the downregulation of tight junction proteins. Moreover, these postbiotics help restore the gut microbiota composition and functionality in colitis mice, with potentially superior regulatory effects compared to sulfasalazine (SASP). Overall, HIC and CFS protect the intestinal barrier function and improve DSS-induced colitis, supporting the development of functional food supplements.

博氏酵母菌的失活细胞和代谢物减轻溃疡性结肠炎小鼠Caco-2单层细胞的炎症损伤。
博拉迪Saccharomyces boulardii (S. boulardii)因其抗菌和抗炎的特性而受到广泛关注。本研究以博氏弧菌热灭活细胞(HIC)和无细胞上清(CFS)为原料制备后生物制剂,并将代谢产物中的重要成分l-精氨酸(Arg)作为实验组之一。结果表明,在lps刺激的Caco-2细胞中,HIC、CFS和Arg通过抑制TNF-α、IL-1β和IL-6的表达,同时增强occludin和ZO-1蛋白的表达,从而保护肠上皮屏障的完整性。在葡聚糖硫酸钠(DSS)诱导的结肠炎小鼠中,HIC、CFS和Arg可减轻体重减轻和结肠损伤等症状,同时抑制促炎因子的上调和紧密连接蛋白的下调。此外,这些后生物制剂有助于恢复结肠炎小鼠肠道微生物群的组成和功能,与磺胺吡啶(SASP)相比,具有潜在的优越调节作用。综上所述,HIC和CFS可保护肠道屏障功能,改善dss性结肠炎,支持功能性食品补充剂的开发。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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