Enhancement of Hypoxia Tolerance of Gibel Carp (Carassius auratus gibelio) via a Ferroporphyrin-Rich Diet.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hualiang Liang, Haifeng Mi, Kai Wang, Mingchun Ren, Lu Zhang, Dongyu Huang, Jiaze Gu
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引用次数: 0

Abstract

Gibel carp (Carassius auratus gibelio) were hypoxia stressed for 12 h after an 8-week FPR nutrient-enriched feeding experiment, which was to evaluate the role of FPR in hypoxic stress in gibel carp (Carassius auratus gibelio). The dissolved oxygen was reduced to a range of 0.6 ± 0.2 mg/L. Results showed that FPR supplementation could maintain the osmotic pressure equilibrium by improving the ion concentrations of plasma including Na+, Ca+ and K+, and Na+/K+-ATPase activity of liver. FPR supplementation could effectively enhance the antioxidant capacity by improving the levels of GPX, SOD, CAT, and GSH, and reduce the level of MDA. FPR supplementation could improve the core gene expressions of Nrf2 signalling pathway including nrf2, sod, ho-1, gpx, and cat. The high levels of FPR supplementation (0.04%) might had a negative effect on immunity. FPR supplementation could improve the expression levels of HIF-1 signalling pathway-related genes to adapt to hypoxia condition including hif-1α, epo, angpt1, vegf, et1, and tfr-1. These results also were supported by higher SR and number of gill mitochondria in FPR supplementation. In general, the appropriate FPR supplementation was 0.01% based on the results of this study and economic cost, which could heighten hypoxic adaptation and SR.

富铁卟啉饲料对异育银鲫缺氧耐受性的提高。
在为期8周的FPR富营养化饲喂试验后,对异育银鲫进行缺氧胁迫12 h,以评价FPR对异育银鲫缺氧应激的影响。溶解氧降至0.6±0.2 mg/L。结果表明,添加FPR可以通过提高血浆Na+、Ca+和K+离子浓度以及肝脏Na+/K+- atp酶活性来维持渗透压平衡。添加FPR可通过提高GPX、SOD、CAT和GSH水平,降低MDA水平,有效增强抗氧化能力。补充FPR可提高Nrf2信号通路核心基因Nrf2、sod、ho-1、gpx和cat的表达。高水平的FPR添加量(0.04%)可能对免疫产生负面影响。补充FPR可以提高HIF-1信号通路相关基因的表达水平,包括HIF-1 α、epo、angpt1、vegf、et1和tfr-1,以适应缺氧条件。这些结果也得到了FPR中较高的SR和鳃线粒体数量的支持。综合本研究结果和经济成本,适宜的FPR添加量为0.01%,可提高低氧适应能力和SR。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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