Journal of experimental animal science最新文献_第5页

Regeneration potential of the submandibular gland after transplantation 颌下腺移植后的再生潜力

Journal of experimental animal science Pub Date : 2002-08-01 DOI: 10.1016/S0939-8600(02)80008-6

S.G. Hakim , I. Lauer , H. Kosmehl , S.J. Zimmermann , P. Sieg

{"title":"Regeneration potential of the submandibular gland after transplantation","authors":"S.G. Hakim , I. Lauer , H. Kosmehl , S.J. Zimmermann , P. Sieg","doi":"10.1016/S0939-8600(02)80008-6","DOIUrl":"10.1016/S0939-8600(02)80008-6","url":null,"abstract":"<div><p>The aim of this study was to evaluate the regeneration potential of the submandibular gland and the associated morphological and functional impairment after a transient warm ischemia as a result of submandibular gland transfer in humans. 42 rabbits were used for the study. After 1.5 h of transient ischemia, submandibular glands were studied histologically in the following 14 days, one month, two and four months in 32 rabbits. Additionally, the glands were functionally evaluated after one and four months in eleven of these rabbits. Ten rabbits were used to establish the scintigraphic method. In the evaluation of the results unpublished data derived from a previous study by our group has also been considered. The Ki-67 index showed a significant increase of the proliferating rate reaching a peak after 14 days (<em>p</em> = 0.006) and still evident after four months involving mostly the terminal ductal system. There was only a slight increase in the tracer uptake of ischemic glands after one month and a more pronounced one after four months.</p><p>It was concluded that initial loss of acinar cells by 1.5 h of ischemia released a regeneration process which stabilised after 14 days but continued throughout the whole time of the study. These results correlate with clinical observation in patients with submandibular gland transfer for the treatment of keratoconjunctivitis sicca and explain the alternating secretory function observed within the four months following surgery. Scintigraphy has been found to play a limited role in the evaluation of the regeneration process.</p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"42 2","pages":"Pages 57-64"},"PeriodicalIF":0.0,"publicationDate":"2002-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(02)80008-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of fibrin on the integration hydroxyapatite coating implants: experimental study in a rabbit model 纤维蛋白对羟基磷灰石涂层植入物影响的兔模型实验研究

Journal of experimental animal science Pub Date : 2002-08-01 DOI: 10.1016/S0939-8600(02)80012-8

M. Morales , R. Navarro , M. Almenara , J.M. Medina , C. Melian , C. Gutierrez

引用次数: 6

Fibrillation potentials in completely denervated rabbit tibialis anterior muscles 完全失神经兔胫骨前肌的纤颤电位

Journal of experimental animal science Pub Date : 2002-08-01 DOI: 10.1016/S0939-8600(02)80013-X

Tatjana Paternostro-Sluga , M. Nuhr , Ch. Kermer , Th. Lang , A. Sabbas , V. Fialka-Moser

{"title":"Fibrillation potentials in completely denervated rabbit tibialis anterior muscles","authors":"Tatjana Paternostro-Sluga , M. Nuhr , Ch. Kermer , Th. Lang , A. Sabbas , V. Fialka-Moser","doi":"10.1016/S0939-8600(02)80013-X","DOIUrl":"10.1016/S0939-8600(02)80013-X","url":null,"abstract":"<div><p>The aim of the study was to assess the quantitative expression and amplitude of fibrillation potentials after 4 months of complete denervation in New Zealand White rabbit tibialis anterior muscles and to compare methods to quantify fibrillation potentials.</p><p>Nine adult female New Zealand White rabbits with completely denervated tibialis anterior muscle due to resection of the peroneal nerve and muscle nerve muscle neurotization without reinnervation were evaluated. Axonal degeneration and muscle denervation were documented by histological examination. Needle electromyography was performed after 4 months of denervation. Quantitative expression of fibrillation potentials was assessed by recording the firing rate per second, grading according to Daube's score for the overall muscle and grading according to a modified score for each recording site. The highest amplitude of each recording site was measured peak to peak. Statistical evaluation was done descriptively and Wilxocon's signet-rank test was performed to compare firing rates between different grades.</p><p>Fifty-nine single recording sites were examined for denervated tibialis anterior muscle. The mean firing rate of FPs for all recording sites was 247.2 ± 96.7 pulses/s. Grading for the overall muscle according to Daube's score showed 3–4+ activity for all muscles. Grading for single recording sites according to the modified score showed 4+ activity in 45.8% of recording sites, 3+ activity in 44,1% and 2+ activity in 10.1%.</p><p>The study provides quantitative electrophysiological data of fibrillation potentials in completely denervated rabbit tibialis anterior muscle and presents methods of quantification.</p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"42 2","pages":"Pages 113-121"},"PeriodicalIF":0.0,"publicationDate":"2002-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(02)80013-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection of a high quality subpopulation of frozen thawed bovine spermatozoa by filtration in a Sephadex column. An assessment of functional effects of progesterone 通过Sephadex柱过滤，选择高质量的冷冻解冻牛精子亚群。黄体酮的功能作用评价

Journal of experimental animal science Pub Date : 2002-08-01 DOI: 10.1016/S0939-8600(02)80011-6

L.M. Vincenti, M. Fiol de Cuneo , R.D. Ruiz , A.C. Martini, A.A. Ponce

{"title":"Selection of a high quality subpopulation of frozen thawed bovine spermatozoa by filtration in a Sephadex column. An assessment of functional effects of progesterone","authors":"L.M. Vincenti, M. Fiol de Cuneo , R.D. Ruiz , A.C. Martini, A.A. Ponce","doi":"10.1016/S0939-8600(02)80011-6","DOIUrl":"10.1016/S0939-8600(02)80011-6","url":null,"abstract":"<div><p>It is accepted that cryopreservation exerts deleterious effects on functional characteristics of mammalian spermatozoa. Conventional procedures for processing frozen-thawed gametes, such as centrifugation, produce additional damage. In the present work, we investigated the efficacy of processing bovine cryopreserved semen by filtration in a Sephadex column (SF group) or by washing by centrifugation (100 g, 10 min, twice) (W group); the results obtained from both procedures were compared to untreated samples (C group). The effects of in vitro addition of progesterone (10 μM, 20 min) upon sperm functional activity were studied also. The evaluated sperm parameters were concentration, motility (progressive or non progressive cells), viability and acrosome reaction. They were measured at time 0 (immediately after processing) or after 4 h incubation in capacitating conditions. Sperm concentration was (× 10<sup>−6</sup>): 37.5 ± 5.4 in C, 8.3 ± 2.1 in W and 12.5 ± 2.9 in SF. The percentages of motile, progressive, viable or acrosome intact gametes were significantly higher in SF than in W or in C. in SF group, after 4 h incubation in capacitating conditions, progesterone increased significantly the population of acrosome reacted cells whereas this parameter was not modified when the cells were incubated in absence of heparin. Motility and viability were not modified by the hormone. We conclude that Sephadex filtration method is an adequate tool to obtain a subpopulation of spermatozoa with superior quality, as assessed by motility, viability and acrosomal integrity; besides, our results strongly support that, as in other species, progesterone would be a physiological inductor of acrosome reaction in bovine.</p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"42 2","pages":"Pages 93-101"},"PeriodicalIF":0.0,"publicationDate":"2002-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(02)80011-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56858123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Immunoaffinity purification and quantitation of a rat protein similar to the human decidua-associated protein (hDP) 71 类似于人蜕膜相关蛋白(hDP) 71的大鼠蛋白的免疫亲和纯化和定量

Journal of experimental animal science Pub Date : 2002-08-01 DOI: 10.1016/S0939-8600(02)80010-4

R. Halperin , E. Hadas , N. Shinnar , P.F. Kraicer , I. Bukovsky , D. Schneider

{"title":"Immunoaffinity purification and quantitation of a rat protein similar to the human decidua-associated protein (hDP) 71","authors":"R. Halperin , E. Hadas , N. Shinnar , P.F. Kraicer , I. Bukovsky , D. Schneider","doi":"10.1016/S0939-8600(02)80010-4","DOIUrl":"10.1016/S0939-8600(02)80010-4","url":null,"abstract":"<div><p>The role of human decidua-associated protein (hDP) 71, copurified consistently with hDP 200, identified as rheumatoid factor, remains undetermined. The possibility of using a rat as an experimental model for the further research of hDp 71, was examined. A rat protein, similar to the hDp 71, was immunoaffinity purified using the same monoclonal antibody recognizing hDp 71. The protein was named rat decidua- associated protein (rDP) 71. The level of hDp 71 in extracts of endometrial epithelium and stroma, as well as in uterine washings, was measured throughout the oestrous cycle and on 5 consecutive days, starting the day after the rats mated. Moreover, the effect of oestrogen and progesterone on the level of hDp 71 was examined. The results demonstrate the oestrogen-dependent accumulation of rDP 71 in uterine lumen, and support the use of a rat as an experimental animal model to investigate the possible physiological role of this protein in the reproductive process.</p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"42 2","pages":"Pages 84-92"},"PeriodicalIF":0.0,"publicationDate":"2002-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(02)80010-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56858066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of α2-macroglobulin concentrations in healthy rats of various ages and rats inoculated with turpentine oil by an enzyme-linked immunosorbent assay 酶联免疫吸附法测定不同年龄健康大鼠和接种松节油大鼠体内α2巨球蛋白的浓度

Journal of experimental animal science Pub Date : 2002-01-01 DOI: 10.1016/S0939-8600(02)80005-0

Seiya Inoue , Tomokazu Jinbo , Masao Shino , Koji Iguchi , Masanori Nomura , Katsuhito Kawato , Shizuo Yamamoto

{"title":"Determination of α2-macroglobulin concentrations in healthy rats of various ages and rats inoculated with turpentine oil by an enzyme-linked immunosorbent assay","authors":"Seiya Inoue , Tomokazu Jinbo , Masao Shino , Koji Iguchi , Masanori Nomura , Katsuhito Kawato , Shizuo Yamamoto","doi":"10.1016/S0939-8600(02)80005-0","DOIUrl":"10.1016/S0939-8600(02)80005-0","url":null,"abstract":"<div><p>An enzyme-linked immunosorbent assay (ELISA) for rat α2-macroglobulin (α2M) was developed, and the concentrations of α2M in sera in normal Crj:CD and Crj:WI rats as well as in turpentine oil injected Crj:WI rats were determined.</p><p>Anti-rat α2M serum was prepared by immunization of rabbits with the α2M fraction separated from rat acute phase serum using Sephacryl S-300. The specificity of the rabbit anti-rat α2M serum was confirmed by immunoelectrophoresis and Western blotting.</p><p>The α2M concentrations in 180 normal Crj:CD (90 males and 90 females) and 180 Crj:WI (90 males and 90 females) rats were 14.5–94.9 μg/ml (mean 36.8 ± 13.3) and 13.6–65.8 μg/ml (mean 34.9 ± 12.4), respectively. There were no significant differences between male and female Crj:CD and Crj:WI rats, respectively (p < 0.01). No significant age-related difference was found in Crj:CD or Crj:WI rats (p < 0.01). The α2M concentrations in sera from 25 Crj:WI rats 48 h after inoculation of turpentine oil were 440–6498 μg/ml (mean 2621 ± 1727).</p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"42 1","pages":"Pages 44-49"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(02)80005-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The forestomach of rats and mice, an effective device supporting digestive metabolism in muridae (review) 大鼠、小鼠前胃:一种有效的支持消化代谢的器官(综述)

Journal of experimental animal science Pub Date : 2002-01-01 DOI: 10.1016/S0939-8600(02)80002-5

Klaus Gärtner

{"title":"The forestomach of rats and mice, an effective device supporting digestive metabolism in muridae (review)","authors":"Klaus Gärtner","doi":"10.1016/S0939-8600(02)80002-5","DOIUrl":"10.1016/S0939-8600(02)80002-5","url":null,"abstract":"<div><p>The forestomach of rats and mice adapts the digestive regime of rats and mice from “food intake in bulks” to a “steady state digestion”. It stores well chewed and salivated food in amounts as needed for one to three hours or longer. The forestomach supplies its content for further digestion independently from its filling degree but in relation to the host's actual energy need during the light period or lipid metabolism during the dark period. Postprandial hyperglycemia and glycogen increase in the liver is lacking.</p><p>Animals ingest most of their daily food during darkness. Meal frequency increases while the amount of food stored in the forestomach is hardly influenced. However, extremely high food demand or limitation of feeding time leads to increased filling per meal of the forestomach by four times and more, but in relation to “eating for calories”.</p><p>The brain's appetite-regulating-network and signals which mechanically record the distension of the forestomach-stomach-cavity determine the ingested volume. In leptin-deficient <em>Lep<sup>ob</sup>/Lep<sup>ob</sup></em> mice the filling volume of the forestomach per meal increases up to five times during the night and twice during the day. The frequency of meals declines.</p><p>Emptying of the forestomach is controlled by a local feed back loop between duodenal reabsorption and the tension of the forestomach muscle fibres linked by humoral signals such as secretin and cholecystokinin.</p><p>During storage in the forestomach food pap is subjected to digestion of starch, fat and chitin, but none of cellulose or protein can be observed. Microorganisms are without significance for the forestomach's function as shown in germ-free animals. Lactobacillae dominate in the variety of organisms lodged there. They are authochthonous and attached to the surface of the wall as dense carpets. Their lactic acid may have preservation effect on the food stored.</p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"42 1","pages":"Pages 1-20"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(02)80002-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Total body electrical conductivity (TOBEC) measurements in Mongolian gerbils (Meriones unguiculatus) and golden hamsters (Mesocricetus auratus) 蒙古沙鼠(Meriones unguiculatus)和金仓鼠(Mesocricetus auratus)全身电导率测定

Journal of experimental animal science Pub Date : 2002-01-01 DOI: 10.1016/S0939-8600(02)80003-7

René Weinandy, Rolf Gattermann

{"title":"Total body electrical conductivity (TOBEC) measurements in Mongolian gerbils (Meriones unguiculatus) and golden hamsters (Mesocricetus auratus)","authors":"René Weinandy, Rolf Gattermann","doi":"10.1016/S0939-8600(02)80003-7","DOIUrl":"10.1016/S0939-8600(02)80003-7","url":null,"abstract":"<div><p>Body composition of adult Mongolian gerbils <em>(Meriones unguiculatus)</em> and golden hamsters <em>(Mesocricetus auratus)</em> was determined using the non-invasive measurement of the total body electrical conductivity (TOBEC). A regression model was developed based on direct chemical analysis of the carcass and calibration equations were defined to predict the fat-free mass (FFM) and the total body water (TBW) in these two species. The closest correlation was obtained by calculating a linear regression of FFM and TBW as a function of TOBEC: gerbils: FFM (g) = 0.20548 × TOBEC + 10.0224; TBW (g) = 0.14485 × TOBEC + 6.51824; golden hamsters: FFM (g) = 0.18043 × TOBEC + 18.5806; TBW (g) = 0.14817 × TOBEC + 4.50379. Based on these equations and the respective TOBEC values, the body composition was analysed. Male golden hamsters had a higher crude fat mass (CFM, obtained by substracting the FFM from the body mass) and a higher dried body mass (DBM, obtained by substracting the TBW from the body mass) than females, which had a higher percentage of FFM and TBW. In Mongolian gerbils, males had higher CFM, higher DBM and the absolute FFM and TBW was elevated. As in female golden hamsters, female gerbils had a higher percentage of FFM and TBW. Results are discussed in relation to the usefulness of this non-invasive technique, which allows repeated individual measurements of body composition. It helps to reduce the number of animals necessary when estimating body condition and welfare issues, male/female differences or for the evaluation of seasonal effects and housing conditions.</p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"42 1","pages":"Pages 21-30"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(02)80003-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Effects of a topical silver sulfadiazine polyurethane dressing (Mikacure) on wound healing in experimentally infected wounds in the pig. A pilot study 外用磺胺嘧啶银聚氨酯敷料(Mikacure)对实验性感染猪伤口愈合的影响。一项初步研究

Journal of experimental animal science Pub Date : 2002-01-01 DOI: 10.1016/S0939-8600(02)80004-9

D. Konrad , M. Tsunoda , K. Weber , S.J. Corney , L. Ullmann

引用次数: 7

Ausschreibung 招标

Journal of experimental animal science Pub Date : 2002-01-01 DOI: 10.1016/S0939-8600(02)80006-2

引用次数: 0