Immunoaffinity purification and quantitation of a rat protein similar to the human decidua-associated protein (hDP) 71

Abstract

The role of human decidua-associated protein (hDP) 71, copurified consistently with hDP 200, identified as rheumatoid factor, remains undetermined. The possibility of using a rat as an experimental model for the further research of hDp 71, was examined. A rat protein, similar to the hDp 71, was immunoaffinity purified using the same monoclonal antibody recognizing hDp 71. The protein was named rat decidua- associated protein (rDP) 71. The level of hDp 71 in extracts of endometrial epithelium and stroma, as well as in uterine washings, was measured throughout the oestrous cycle and on 5 consecutive days, starting the day after the rats mated. Moreover, the effect of oestrogen and progesterone on the level of hDp 71 was examined. The results demonstrate the oestrogen-dependent accumulation of rDP 71 in uterine lumen, and support the use of a rat as an experimental animal model to investigate the possible physiological role of this protein in the reproductive process.