Regeneration potential of the submandibular gland after transplantation

S.G. Hakim , I. Lauer , H. Kosmehl , S.J. Zimmermann , P. Sieg
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Abstract

The aim of this study was to evaluate the regeneration potential of the submandibular gland and the associated morphological and functional impairment after a transient warm ischemia as a result of submandibular gland transfer in humans. 42 rabbits were used for the study. After 1.5 h of transient ischemia, submandibular glands were studied histologically in the following 14 days, one month, two and four months in 32 rabbits. Additionally, the glands were functionally evaluated after one and four months in eleven of these rabbits. Ten rabbits were used to establish the scintigraphic method. In the evaluation of the results unpublished data derived from a previous study by our group has also been considered. The Ki-67 index showed a significant increase of the proliferating rate reaching a peak after 14 days (p = 0.006) and still evident after four months involving mostly the terminal ductal system. There was only a slight increase in the tracer uptake of ischemic glands after one month and a more pronounced one after four months.

It was concluded that initial loss of acinar cells by 1.5 h of ischemia released a regeneration process which stabilised after 14 days but continued throughout the whole time of the study. These results correlate with clinical observation in patients with submandibular gland transfer for the treatment of keratoconjunctivitis sicca and explain the alternating secretory function observed within the four months following surgery. Scintigraphy has been found to play a limited role in the evaluation of the regeneration process.

颌下腺移植后的再生潜力
本研究的目的是评估人类颌下腺移植后短暂热缺血后颌下腺的再生潜力和相关的形态学和功能损伤。实验用42只家兔。32只兔短暂缺血1.5 h后,分别于14天、1个月、2个月和4个月对颌下腺进行组织学观察。此外,在1个月和4个月后,对其中11只兔子的腺体进行了功能评估。以10只家兔为实验对象,建立扫描法。在评估结果时,还考虑了我们小组先前研究中未发表的数据。Ki-67指数显示增殖率显著升高,在14天后达到峰值(p = 0.006), 4个月后仍明显,主要累及末端导管系统。一个月后,缺血腺体的示踪剂摄取仅略有增加,四个月后更明显。由此得出结论,在缺血1.5小时后,腺泡细胞的初始损失释放了一个再生过程,该过程在14天后稳定下来,并在整个研究期间持续进行。这些结果与临床观察的颌下腺转移治疗干燥性角膜结膜炎患者的相关,并解释了手术后4个月内观察到的交替分泌功能。人们发现闪烁成像在评价再生过程中起着有限的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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