Determination of α2-macroglobulin concentrations in healthy rats of various ages and rats inoculated with turpentine oil by an enzyme-linked immunosorbent assay

Journal of experimental animal science Pub Date : 2002-01-01 DOI:10.1016/S0939-8600(02)80005-0

Seiya Inoue , Tomokazu Jinbo , Masao Shino , Koji Iguchi , Masanori Nomura , Katsuhito Kawato , Shizuo Yamamoto

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引用次数: 7

Abstract

An enzyme-linked immunosorbent assay (ELISA) for rat α2-macroglobulin (α2M) was developed, and the concentrations of α2M in sera in normal Crj:CD and Crj:WI rats as well as in turpentine oil injected Crj:WI rats were determined.

Anti-rat α2M serum was prepared by immunization of rabbits with the α2M fraction separated from rat acute phase serum using Sephacryl S-300. The specificity of the rabbit anti-rat α2M serum was confirmed by immunoelectrophoresis and Western blotting.

The α2M concentrations in 180 normal Crj:CD (90 males and 90 females) and 180 Crj:WI (90 males and 90 females) rats were 14.5–94.9 μg/ml (mean 36.8 ± 13.3) and 13.6–65.8 μg/ml (mean 34.9 ± 12.4), respectively. There were no significant differences between male and female Crj:CD and Crj:WI rats, respectively (p < 0.01). No significant age-related difference was found in Crj:CD or Crj:WI rats (p < 0.01). The α2M concentrations in sera from 25 Crj:WI rats 48 h after inoculation of turpentine oil were 440–6498 μg/ml (mean 2621 ± 1727).

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酶联免疫吸附法测定不同年龄健康大鼠和接种松节油大鼠体内α2巨球蛋白的浓度

采用酶联免疫吸附法(ELISA)检测大鼠α2-巨球蛋白(α2M)，测定正常Crj:CD、Crj:WI大鼠血清中α2M的含量以及注射松节油的Crj:WI大鼠血清中α2M的含量。用Sephacryl S-300从大鼠急性期血清中分离α2M部分，免疫兔制备抗大鼠α2M血清。兔抗大鼠α2M血清的特异性经免疫电泳和Western blotting证实。180只正常Crj:CD大鼠(公母各90只)和180只Crj:WI大鼠(公母各90只)α2M浓度分别为14.5 ~ 94.9 μg/ml(平均36.8±13.3)和13.6 ~ 65.8 μg/ml(平均34.9±12.4)。Crj:CD大鼠和Crj:WI大鼠雌雄间差异均无统计学意义(p <0.01)。Crj:CD和Crj:WI大鼠无明显年龄相关差异(p <0.01)。25只Crj:WI大鼠接种松节油48 h后血清α2M浓度为440 ~ 6498 μg/ml(平均2621±1727)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of experimental animal science

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