Journal of experimental animal science最新文献_第6页

Computerised animal house management system (CAMS) 电脑化动物舍管理系统(CAMS)

Journal of experimental animal science Pub Date : 2000-12-01 Epub Date: 2005-08-16 DOI: 10.1016/S0939-8600(01)80001-8

P. Hempel , R. Merx , M. Schulz , U. Zillmann

{"title":"Computerised animal house management system (CAMS)","authors":"P. Hempel , R. Merx , M. Schulz , U. Zillmann","doi":"10.1016/S0939-8600(01)80001-8","DOIUrl":"10.1016/S0939-8600(01)80001-8","url":null,"abstract":"<div>Data processing systems for the management of animal houses are available as individually tailored solutions to meet the specific requirements of different institutions in various sectors. After four years of use, a “proprietary system” (originally used by Boehringer/Mannheim), which was based on UNIFACE and ORACLE database software and consisted of a VAX station with eight terminals, was replaced by a PC-based multi-user system for administrative employees and animal technicians. The new system runs under the operating systems of Microsoft Windows 98 and Microsoft Windows NT and uses Microsoft Access 97 as the database software. A “multipurpose program system” was created in order to fulfil the following main functions: documentation and control of experimental plans and animal usage; registering changes in the number of animals available; controlling room use and animal storage areas; maintenance cost calculations for determining the cost of experiments and an evaluation of how animals are used and consumed.CAMS (Computerised Animal House Management System) has a centralised / decentralised structure and is a user-friendly, interactive system which can only be accessed by authorised groups of users. You do not need to be highly skilled with computers to master the system. It can generate daily and monthly reports and any necessary documentation. Furthermore, it complies with the revised Animal Protection Act (1998; annual report on number of animals used), facilitates a detailed analysis of housing and the associated costs and provides options for documenting different types of experimental data.For this reason, the system has become an increasingly important tool for the management of our own animal facilities and animal experiments as well as for external ones.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 4","pages":"Pages 149-158"},"PeriodicalIF":0.0,"publicationDate":"2000-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(01)80001-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The endocrine pancreas in the adult gray short-tailed opossum, Monodelphis domestica (Marsupialia) 成年灰短尾负鼠(有袋目)的内分泌胰腺

Journal of experimental animal science Pub Date : 2000-12-01 Epub Date: 2005-08-16 DOI: 10.1016/S0939-8600(01)80004-3

E. Engelke, I. Peukert-Adam, H. Gasse

{"title":"The endocrine pancreas in the adult gray short-tailed opossum, Monodelphis domestica (Marsupialia)","authors":"E. Engelke, I. Peukert-Adam, H. Gasse","doi":"10.1016/S0939-8600(01)80004-3","DOIUrl":"10.1016/S0939-8600(01)80004-3","url":null,"abstract":"<div>Monodelphis domestica, a South American marsupial proposed as a laboratory animal, presents some characteristics that are favourable for research on the development of pancreatic tissue. Therefore, it is important to know if Monodelphis domestica is similar to other mammals concerning the cellular composition of the pancreas and if the different regions of the organ share similar morphologic features. In the present study the pancreas of ten adult male or female Monodelphis domestica were examined: serial paraffin sections were stained immunohistochemically using polyclonal antibodies against insulin or glucagon. The size of the immunoreactive area was measured with a computer-based image-analysing system differentiating lobus dexter, corpus pancreatis, and lobus sinister.The islets' profiles varied in size and shape. The insulin-positive cells were mainly located in the centre of the islets, while the glucagon-positive cells were arranged at the islets' periphery. However, some glucagon-positive cells also occurred in the centre of most islets. On average, 0.66% of the entire pancreatic section area was insulin-positive, while 0.23% was glucagon-positive. Thus, the ratio between glucagon-positive and insulin-positive area was 1:2.9. Differences between the three pancreatic parts, lobus dexter, corpus pancreatis, and lobus sinister, were revealed. In general, the findings compared well with those of species that have a history as laboratory animals, i.e. mice, rats, cats, and dogs.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 4","pages":"Pages 176-184"},"PeriodicalIF":0.0,"publicationDate":"2000-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(01)80004-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The gamma-delta T-cell cytokine response to peritonitis - δ t细胞因子对腹膜炎的反应

Journal of experimental animal science Pub Date : 2000-12-01 Epub Date: 2005-08-16 DOI: 10.1016/S0939-8600(01)80002-X

Ramon Berguer , David Ferrick

引用次数: 0

MRI and biochemical investigation of the temporomandibular joint of juvenile pigs after sagittal advancement of the mandibulae 幼年猪下颌骨矢状突进后颞下颌关节的MRI和生化研究

Journal of experimental animal science Pub Date : 2000-12-01 Epub Date: 2005-08-16 DOI: 10.1016/S0939-8600(01)80003-1

T. Gedrange , K. Winnefeld , V. Hietschold , H. Schubert , W. Harzer

{"title":"MRI and biochemical investigation of the temporomandibular joint of juvenile pigs after sagittal advancement of the mandibulae","authors":"T. Gedrange , K. Winnefeld , V. Hietschold , H. Schubert , W. Harzer","doi":"10.1016/S0939-8600(01)80003-1","DOIUrl":"10.1016/S0939-8600(01)80003-1","url":null,"abstract":"<div>Orthodontic treatment leads to changes of the temporomandibular joint (TMJ). The extent and manner of alterations are not yet clarified completely. The non-invasive diagnostic methods do not provide any precise data about the extent of alterations. The aim of the present investigation was to analyse the TMJ cartilage after orthodontic treatment with non-invasive and invasive methods.For the present investigation, twelve 10-week-old pigs including six control animals were used. Treatment was carried out with build-ups on the molars for sagittal advancement of the mandibulae. Magnetic resonance imaging (MRI) and determination of the Ca++ and Mg++ content were carried out 4 weeks after implantation of the build-ups. Tissue samples for ion determination were taken from the posterior area of the TMJ (condyle and fossa). During condylar growth a significant increase in cartilage volume and decrease of Ca++ and Mg++ content was observed. The newly formed tissue had a water content of 80–90%. On MR images of animals with build-ups this area was visible as a bright zone with increased signal intensity.The determined stress potential of the condylar cartilage was reduced as a result of less functional stress in the posterior area accompanied by water intake. The newly formed cartilage matrix contained significantly less Ca++ and Mg++, and cannot be defined as permanently stable cartilage.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 4","pages":"Pages 168-175"},"PeriodicalIF":0.0,"publicationDate":"2000-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(01)80003-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Comparison of the postoperative analgesic effects of buprenorphine and piritramide following experimental orthopaedic surgery in sheep 丁丙诺啡与匹利胺在绵羊实验性骨科术后镇痛效果的比较

Journal of experimental animal science Pub Date : 2000-09-01 Epub Date: 2005-08-16 DOI: 10.1016/S0939-8600(00)80005-X

K.A. Otto , K.H.S. Steiner , F. Zailskas , B. Wippermann

{"title":"Comparison of the postoperative analgesic effects of buprenorphine and piritramide following experimental orthopaedic surgery in sheep","authors":"K.A. Otto , K.H.S. Steiner , F. Zailskas , B. Wippermann","doi":"10.1016/S0939-8600(00)80005-X","DOIUrl":"10.1016/S0939-8600(00)80005-X","url":null,"abstract":"<div>Summary<ul><li>•Introduction: Long-acting opioids may be needed for postoperative pain relief in sheep. Reports on the analgesic efficacy of the partial μ agonist buprenorphine, however, are controversial. Therefore, the aim of this study was to compare the effects of buprenorphine with the μ full agonist piritramide on clinical pain following orthopaedic surgery in sheep.</li><li>•Material and methods: Postoperative analgesia of intramuscular buprenorphine (0.01 mg/kg) and piritramide (0.57 mg/kg) were evaluated in a prospective, randomised and blinded study in 22 ewes submitted to an experimental tibia osteotomy. For evaluation of postoperative analgesia the time from extubation to first attempt to stand, to standing, to first food/water uptake, to first postoperative analgesic treatment, the number of animals treated per group, postoperative behaviour and lameness scores were compared.</li><li>•Results: The distribution of behavioural scores in standing/walking animals was significantly different (P = 0.018) between the two treatment groups. In the buprenorphine group behaviour scores ranged more in the lower score range while higher scores were recorded in the piritramide group. In both groups the same number of nine sheep required additional postoperative analgesic treatment at almost identical time intervals. No significant differences existed for the other variables.</li><li>•Conclusion: Buprenorphine and piritramide provided effective analgesia following tibia osteotomy in sheep with a slightly better quality in animals pre-treated with buprenorphine.</li></ul></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 3","pages":"Pages 133-143"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80005-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56855916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The chronic proliferative dermatitis mouse mutation (cpdm): mapping of the mutant gene locus 慢性增生性皮炎小鼠突变(cpdm):突变基因位点的定位

Journal of experimental animal science Pub Date : 2000-09-01 Epub Date: 2005-08-16 DOI: 10.1016/S0939-8600(00)80001-2

John P. Sundberg , Dawnalyn Boggess , Leonard D. Shultz , Remond J.A. Fijneman , Peter Demant , Harm Hogenesch , Gregory A. Cox

引用次数: 4

First announcement 第一次公告

Journal of experimental animal science Pub Date : 2000-09-01 Epub Date: 2005-08-16 DOI: 10.1016/S0939-8600(00)80006-1

引用次数: 0

Postoperative nutrition of Göttingen minipigs after 2/3 gastric resection Göttingen迷你猪2/3胃切除术后的术后营养

Journal of experimental animal science Pub Date : 2000-09-01 Epub Date: 2005-08-16 DOI: 10.1016/S0939-8600(00)80002-4

J. Metzger , R. Weilenmann , L. Degen , M. von Flüe

引用次数: 2

Ausschreibung 招标

Journal of experimental animal science Pub Date : 2000-09-01 Epub Date: 2005-08-16 DOI: 10.1016/S0939-8600(00)80007-3

引用次数: 0

Percutaneous liver core biopsy in rats: an alternative to minilaparotomy 大鼠经皮肝核心活检:小切口的替代方法

Journal of experimental animal science Pub Date : 2000-09-01 Epub Date: 2005-08-16 DOI: 10.1016/S0939-8600(00)80003-6

Gerald Drews , Kay Kohlhaw , Daniel Palmes , Petra Madaj-Sterba , Thomas Hartwig , Johann Hauss , Hans-Ullrich Spiegel

{"title":"Percutaneous liver core biopsy in rats: an alternative to minilaparotomy","authors":"Gerald Drews , Kay Kohlhaw , Daniel Palmes , Petra Madaj-Sterba , Thomas Hartwig , Johann Hauss , Hans-Ullrich Spiegel","doi":"10.1016/S0939-8600(00)80003-6","DOIUrl":"10.1016/S0939-8600(00)80003-6","url":null,"abstract":"<div>Sampling of rat hepatic tissue for histomorphological analysis is usually performed in two different ways: either by killing the animals or by minilaparotomy.In this study we describe a percutaneous core biopsy technique which has been used on day 7, 14 and 21 after allogeneic rat liver transplantation (DA → LEW) in order to examine grafts for rejection in different treatment groups. Fifty-two liver biopsies were performed in 24 animals using a 16-gauge intravenous cannula. Forty-five provided usable specimens which were sufficient for both light or electron microscopy and immunohistochemical analyses to determine the degree of graft rejection. In 7 cases (13.5%) sampling was unsuccessful, especially on day 21 after transplantation, as the plastic cannula could not penetrate the hardened tissue. In 3 animals (5.8%) puncture was immediately followed by death due to perforation of the diaphragm or ether intoxication.In conclusion, this technique is a reliable method for providing ample tissue samples from the rat liver with a low risk of complications.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 3","pages":"Pages 115-120"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80003-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56856348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1