Journal of experimental animal science最新文献_第7页

Hemodynamic and electroencephalographic effects of epidural buprenorphine during orthopedic hindlimb surgery in sheep: A comparison with intramuscular buprenorphine and epidural saline 硬膜外丁丙诺啡对绵羊矫形后肢手术中血流动力学和脑电图的影响:与肌注丁丙诺啡和硬膜外生理盐水的比较

Journal of experimental animal science Pub Date : 2000-09-01 DOI: 10.1016/S0939-8600(00)80004-8

Klaus A. Otto , Thomas Gerich , Christhild Volmert

{"title":"Hemodynamic and electroencephalographic effects of epidural buprenorphine during orthopedic hindlimb surgery in sheep: A comparison with intramuscular buprenorphine and epidural saline","authors":"Klaus A. Otto , Thomas Gerich , Christhild Volmert","doi":"10.1016/S0939-8600(00)80004-8","DOIUrl":"10.1016/S0939-8600(00)80004-8","url":null,"abstract":"<div>Epidural opioids exert segmentally limited spinal analgesia by acting at dorsal horn neurons. While the spinal cord may be the predominant site of action for hydrophilic opioids such as morphine, initial supraspinal effects have been suggested for lipophilic opioids such as buprenorphine. In view of significant systemic effects, the value of epidural administration of lipophilic opioids in human patients has been questioned. Since epidural buprenorphine may be beneficial for hindlimb surgeries in sheep, intraoperative hemodynamic and central nervous effects were evaluated. In a prospective, randomized, and placebo-controlled study, 15 adult sheep anesthetized for cranial cruciate ligament reconstruction were treated with either epidural buprenorphine (5 μg/kg, n = 5), intramuscular buprenorphine (5 μg/kg, n = 5), or epidural saline (0.15 ml/kg, n = 5) preoperatively. Heart rate, arterial blood pressures and the electroencephalographic variables ϑ/δ ratio, α/δ ratio, β/δ ratio, median power frequency (MED), and 80% spectral edge frequency were recorded before and immediately after skin incision and during drilling a tunnel through the tibia bone. Arterial pressures after epidural buprenorphine were significantly lower compared with epidural saline but were quite similar to intramuscular buprenorphine before skin incision. Mean EEG ϑ/δ ratios and MED values following epidural and intramuscular buprenorphine were significantly lower compared with epidural saline but quite similar for the two buprenorphine groups during drilling. In conclusion, similar hemodynamic and electroencephalographic effects of epidural and intramuscular buprenorphine suggest systemic effects of epidural buprenorphine in sheep.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 3","pages":"Pages 121-132"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80004-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56856366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Loci of the immune system are implicated in diabetes frequency and age at onset of diabetes in BB rats 在BB大鼠中，免疫系统的基因座与糖尿病的发病频率和年龄有关

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80020-6

I. Klöting, P. Kovács

引用次数: 0

Wild rats (Rattus norvegicus) for mapping of disease-resistant genes 野生大鼠(褐家鼠)抗病基因定位

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80031-0

J. van den Brandt, P. Kovács, I. Klöting

{"title":"Wild rats (Rattus norvegicus) for mapping of disease-resistant genes","authors":"J. van den Brandt, P. Kovács, I. Klöting","doi":"10.1016/S0939-8600(00)80031-0","DOIUrl":"10.1016/S0939-8600(00)80031-0","url":null,"abstract":"<div>Wild rat representing a disease-resistant phenotype and genotype, was used in a crossing study with spontaneously hypertensive rat (SHR) to search for quantitative trait loci (QTL) affecting blood pressure. Therefore, one male wild rat was crossed with SHR females and F1 hybrids were transferred in a pathogen free environment by wet-hysterectomy and backcrossed onto hypertensive SHR rats resulting in first backcross hybrids (BC1). Considering that the F1 hybrids are not uniform, as are the cross hybrids of inbred rat strains, we selected 72 BC1 progeny of one F1 female, which were characterised for systolic blood pressure, measured by tail cuff method and were genetically analysed using 200 microsatellites covering the whole genome. We found suggestive linkage of blood pressure to region on chromosome 2 flanked by D2Mit8 and Fgg loci (lod score 2.3). In addition, possible interaction between genes on chromosomes 7 and 3, X and 3, 14 and 3, 13 and 11 was described, indicating that blood pressure development in the SHR might be the result of interacting genes.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 1","pages":"Pages 57-60"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80031-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A new rat model for insulin-dependent diabetes mellitus (Iddm) 胰岛素依赖型糖尿病(Iddm)大鼠模型的建立

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80019-X

C.-M. Prokop , D. Wedekind , S. Lenzen , M. Tiedge , M. Elsner , S. Lortz , A. Jörns , G. Klöppel , H.J. Hedrich

引用次数: 1

Derivation of SHR-chromosome 4 congenic sublines for fine genetic mapping of quantitative trait loci with major effects on insulin resistance and blood pressure 对胰岛素抵抗和血压有重要影响的数量性状位点进行精细遗传定位的shr - 4染色体同源亚群的推导

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80027-9

Michal Pravenec , Vladimir Křen , Drahomira Krenova , Vaclav Zidek , Jiaming Wang , Theodore W. Kurtz , Elizabeth St. Lezin

{"title":"Derivation of SHR-chromosome 4 congenic sublines for fine genetic mapping of quantitative trait loci with major effects on insulin resistance and blood pressure","authors":"Michal Pravenec , Vladimir Křen , Drahomira Krenova , Vaclav Zidek , Jiaming Wang , Theodore W. Kurtz , Elizabeth St. Lezin","doi":"10.1016/S0939-8600(00)80027-9","DOIUrl":"10.1016/S0939-8600(00)80027-9","url":null,"abstract":"<div>Recently, we have found that transfer of a segment of chromosome 4 between I16 and Npy markers from the Brown Norway (BN) rat into the spontaneously hypertensive rat (SHR) significantly attenuated both hypertension (measured by telemetry) and insulin resistance (measured as plasma insulin/glucose ratios before and after a high fructose diet) in the SHR progenitor strain. To map the putative quantitative trait loci (QTL) more precisely, we derived an (SHR×SHR.BN-chr.4)F2 population to search for recombinants that will enable us to produce congenic sublines. The F2 animals were genotyped in markers equally distributed along the interval of the chromosome 4 differential segment. Altogether, five new congenic sublines with overlapping segments of the differential chromosome 4 are being produced. New congenic sublines will enable us to test the hypothesis that insulin resistance and hypertension can be influenced by closely linked genes or perhaps even the same gene(s) on chromosome 4.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 1","pages":"Pages 44-46"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80027-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56856904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characterization of expressed RT1-B/D alleles 表达的RT1-B/D等位基因的特征

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80039-5

D. Wedekind, H.J. Hedrich

引用次数: 1

Alloantigen profile of graft vascular endothelial cells in rat heart allograft rejection: expression of MHC and non-MHC alloantigens 移植血管内皮细胞在大鼠心脏移植排斥反应中的异体抗原谱:MHC和非MHC异体抗原的表达

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80035-8

Adrian M. Duijvestijn, Josien G. Derhaag, Maria A.M. Vroomen, Harry van der Heijden, Peter J.C. van Breda Vriesman

{"title":"Alloantigen profile of graft vascular endothelial cells in rat heart allograft rejection: expression of MHC and non-MHC alloantigens","authors":"Adrian M. Duijvestijn, Josien G. Derhaag, Maria A.M. Vroomen, Harry van der Heijden, Peter J.C. van Breda Vriesman","doi":"10.1016/S0939-8600(00)80035-8","DOIUrl":"10.1016/S0939-8600(00)80035-8","url":null,"abstract":"<div>The alloantigen profile of vascular endothelial cells has been studied in a rat experimental model for MHC-mismatched and MHC-matched (non-MHC mismatched) allogeneic heart transplantation (TX). Hearts were studied for alloantibody deposition in the graft vasculature and the occurrence of vasculopathy. In addition reactivity of allosera with in vitro cultures of graft-type vascular endothelial cells was examined. Alloantibodies were seen deposited in the graft vasculature in MHC-mismatched as well as in MHC-matched TX. In MHC-matched hearts the most dominant vasculopathy consisted of early arteriosclerotic lesions. In vitro studies demonstrated that, in addition to MHC alloantigens, heart vascular endothelial cells express non-MHC alloantigens. These endothelial non-MHC alloantigens are constitutively expressed by heart endothelial cells, and expression is not influenced by cytokine stimulation. We conclude that humoral immune response against these endothelial non-MHC alloantigens may play an important role in development of graft vasculopathy and particularly of arteriosclerotic lesions.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 1","pages":"Pages 78-81"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80035-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of the structure and chromosomal assignment of CD1: an evidence for different evolutionary histories between classic CD1 and CD1D class genes CD1的结构和染色体分配的比较分析:经典CD1和CD1D类基因之间不同进化历史的证据

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80037-1

Akihiro Matsuura , Miyuki Kinebuchi , Shigeo Katabami , Chen Hong-Zhi , Kiyoshi Kasai , Shingo Ichimiya , Kazuhiko Yamada , Michihiro C. Yoshida , Masato Horie , Noriyuki Sato

{"title":"Comparative analysis of the structure and chromosomal assignment of CD1: an evidence for different evolutionary histories between classic CD1 and CD1D class genes","authors":"Akihiro Matsuura , Miyuki Kinebuchi , Shigeo Katabami , Chen Hong-Zhi , Kiyoshi Kasai , Shingo Ichimiya , Kazuhiko Yamada , Michihiro C. Yoshida , Masato Horie , Noriyuki Sato","doi":"10.1016/S0939-8600(00)80037-1","DOIUrl":"10.1016/S0939-8600(00)80037-1","url":null,"abstract":"<div>The non-MHC-encoded CD1 family has recently emerged as a novel antigen-presenting system that is distinct from MHC class I and class II molecules. In the present study, we determined the genomic structure of that rat CD1, and compared with those of other previously reported CD1 genes. Rat CD1 was extremely similar to mouse CD1 genes, especially to CD1D1. It is of interest that a tyrosine-based motif for endosomal localization, identified in the human CD1b cytoplasmic tail, was conserved in all CD1 molecules except for CD1a, that was encoded by a single short exon. Comparison of the overall exon-intron organization of CD1 genes revealed that the length of the introns was also characteristic to each of the two classes of CD1 genes; classic (CD1A, CD1B, CD1C and CD1E), and CD1D, which have been categorized by comparison of coding regions. These findings support a hypothesis that the two classes have different evolutionary histories. In contrast to the absence of the classic CD1 genes in rats and mice, the entire region of nonpolymorphic CD1D gene has been conserved through mammalian evolution. Furthermore, we determined chromosomal localization of rat CD1 gene using the fluorescence in situ hybridization method with several probes derived from genomic rat CD1 clones. Similar to human and mouse CD1, rat CD1 mapped outside the MHC loci despite the structural and functional resemblance to MHC. Conserved syntheny of chromosomal segments of RNO2 and MMU3 is implied.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 1","pages":"Pages 87-90"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80037-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Genetic control of arthritis in rats 大鼠关节炎的遗传控制

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80018-8

Rikard Holmdahl , Carina Vingsbo-Lundberg , Niklas Nordquist , Peter Olofsson , Mats Sundvall , Tore Saxne , Ulf Pettersson

{"title":"Genetic control of arthritis in rats","authors":"Rikard Holmdahl , Carina Vingsbo-Lundberg , Niklas Nordquist , Peter Olofsson , Mats Sundvall , Tore Saxne , Ulf Pettersson","doi":"10.1016/S0939-8600(00)80018-8","DOIUrl":"10.1016/S0939-8600(00)80018-8","url":null,"abstract":"<div>This study was specifically designed to analyse the genetic control of the chronic disease course for the development of arthritis. Arthritis models with a chronic erosive arthritis are collagen induced arthritis induced with homologous collagen in oil but also arthritis induced with certain non-immunogenic adjuvants such as pristane and avridine. In the presently described experiment we have used pristane induced arthritis. A single injection of 150 μl pristane induces severe chronic arthritis in DA rats. The disease mimics rheumatoid arthritis in many aspects such as the chronic disease course, an erosive inflammation of peripheral joints, symmetric involvement of the joints and the development of rheumatoid factors. To determine the genetic contribution we have used a number of inbred, recombinant inbred and congenic strains as well as specifically designed segregating crosses. An influence by the MHC region (designated Pia1 locus) on the chronic disease course was determined through the uses of MHC congenic LEW strains in which the RT1-f haplotype conferred highest susceptibility. To map genes outside of MHC we used an F2 cross between the highly susceptible DA and the resistant E3 strains. Loci exclusively associated with different phenotypes of the disease could be identified:<ul><li>Arthritis onset (Pia2 and Pia3).</li><li>Severity and joint erosions (Pia4).</li><li>Chronicity (Pia5 and Pia6) and Pia1 (determined from MHC congenic (strains)</li></ul>These findings demonstrates that a chronic self-perpetuative disease, mimicking rheumatoid arthritis, is controlled by different set of genes exclusively linked to different phases of the disease course such as arthritis onset, joint erosions, severity and chronicity.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 1","pages":"Pages 7-13"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80018-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56856422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microsatellite marker analysis of allelic imbalance in a rat fibrosarcoma model 大鼠纤维肉瘤模型中等位基因失衡的微卫星标记分析

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80022-X

Å. Sjöling, A. Walentinsson, G. Levan, K. Klinga-Levan

{"title":"Microsatellite marker analysis of allelic imbalance in a rat fibrosarcoma model","authors":"Å. Sjöling, A. Walentinsson, G. Levan, K. Klinga-Levan","doi":"10.1016/S0939-8600(00)80022-X","DOIUrl":"10.1016/S0939-8600(00)80022-X","url":null,"abstract":"<div>Fibrosarcomas were induced by subcutaneous injections of DMBA (7,12-dimethylbenz[a]-anthracene) in progeny from a cross between the inbred rat strains BN/Han and LE/Mol. Twenty-one of 34 treated animals (62%) developed fibrosarcomas at the injection site. The tumor DNA was analyzed for allelic imbalance that would be indicative of amplification or deletion of genetic material. In order to cover the entire length of the rat linkage map, including all 20 autosomes and the X chromosome, 215 polymorphic microsatellite markers were selected. The markers were grouped into panels of 6–12 markers, which could be analyzed simultaneously on polyacrylamide gels after fluorescent PCR amplification. Numerous cases of allelic imbalance were revealed using the panels on the fibrosarcoma material. Most of them involved markers situated on rat chromosome 1 and 2, suggesting that genes located on these chromosomes are important in sarcomagenesis. The development of microsatellite marker panels such as those described will greatly facilitate future analysis of rat tumors by genome-wide scanning.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 1","pages":"Pages 27-31"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80022-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56856733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1