Journal of experimental animal science最新文献_第8页

Phenotypic selection: a successful strategy to fix major genes of hypertension 表型选择:固定高血压主要基因的成功策略

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80032-2

P. Kovács, J. van den Brandt, I. Klöting

{"title":"Phenotypic selection: a successful strategy to fix major genes of hypertension","authors":"P. Kovács, J. van den Brandt, I. Klöting","doi":"10.1016/S0939-8600(00)80032-2","DOIUrl":"10.1016/S0939-8600(00)80032-2","url":null,"abstract":"<div>Spontaneously diabetic BB/OK rats are not genetically susceptible to develop diabetic complications as hypertension or nephropathy. Recently, we generated 5 congenic BB. SHR rat strains by transferring different chromosomal regions of the spontaneously hypertensive rat (SHR) onto the genetic background of BB/OK rats. Four out of 5 strains showed a weak increase of blood pressure (8 mmHg). This weak blood pressure effect indicated that the transferred regions fo not contain major genes for hypertension. That prompted us to choose the classical procedure of phenotypic selection to fix major genes causing hypertension in a BB/OK rat subline generated by cross of BB/OK and SHR and repeated backcrossing of animals with highest blood pressure onto normotensive BB/OK rats. After 7 backcrosses (N8), all backcross parents were genetically analysed with the aid of 259 microsatellites to identify loci causing blood pressure of 177 ± 10 mmHg in this BB/OK rat subline. The data revealed, that loci on chromosome 1, 14 and 18 were heterozygous until BC5, BC6 and BC7, respectively. Considering the relative stable high blood pressure during the backcross procedure, these loci might be of essential importance for the development of hypertension in the SHR.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 1","pages":"Pages 61-63"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80032-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84098639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic analysis of hypertension in the Dahl salt-sensitive rat 达尔盐敏感大鼠高血压的遗传分析

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80017-6

J.P. Rapp

引用次数: 0

New tools for the high throughput characterization of rat genomic DNA samples 大鼠基因组DNA样品高通量表征的新工具

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80024-3

M. Knoblauch , C. Gösele , H. Zimdahl , B. Hieke , H. Himmelbauer , L. Schalkwyk , K. Lindpaintner , D. Ganten , H. Lehrach

引用次数: 1

Ausschreibung 招标

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80041-3

引用次数: 0

Genetics of rat hypodactyly 大鼠指下畸形的遗传学

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80028-0

Drahomíra Křenová , Zuzana Jirsová , Vlasta Bílá , Rudolf Kašpárek , Michal Pravenec , Vladimír Křen

{"title":"Genetics of rat hypodactyly","authors":"Drahomíra Křenová , Zuzana Jirsová , Vlasta Bílá , Rudolf Kašpárek , Michal Pravenec , Vladimír Křen","doi":"10.1016/S0939-8600(00)80028-0","DOIUrl":"10.1016/S0939-8600(00)80028-0","url":null,"abstract":"<div>Rat hypodactyly was originally described by Moutier et al. (1973) as an autosomal recessive trait. The determining gene Hd has been recently mapped to rat chromosome 10 and is closely linked to the D10Rat31/32, D10Rat30, and Myh3 loci (Křenová et al. 1998). In homozygous state (Hd/Hd), there is a variable reduction in the number of fingers and metacarpals — metatarsals of front and hind feet in males and females. Moreover, there is a male sterility in homozygotes whereas male heterozygotes are fertile. The light and electron microscopic examination confirmed disorder of spermatogenesis, loosening and vacuolization of seminiferous epithelium accompanied with a significantly decreased number of germ cells in testes of homozygotes.In an intercross population (Wistar Hd×BN-Lx)F2, an independent segregation of the major genes Lx, coding for the PLS (polydactyly-luxate syndrome), and Hd — hypodactyly was found together with irregular interactions of Hd and Lx genes in double homozygotes (Hd/Hd, Lx/Lx). The variable phenotype manifestation of foot malformation in double homozygous animals indicated modifying influences of genes of the genetic background. In order to study more precisely the role of the determining major gene Hd as well as the role of the putative modifying genes in the development of the foot malformation and male sterility, we started the production of two congenic strains by introgressing the Hd mutant gene onto the genetic backgrounds of the BN and SHR inbred strains.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 1","pages":"Pages 47-50"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80028-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56856916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Polygenetic susceptibility and resistance to 4-nitroquinoline 1-oxide-induced tongue carcinomas in the rat 4-硝基喹啉1-氧化物诱导大鼠舌癌的多遗传易感性和抗性

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80034-6

Jun-ichi Tanuma , Motoo Kitano , Hayase Shisa , Hiroshi Hiai

{"title":"Polygenetic susceptibility and resistance to 4-nitroquinoline 1-oxide-induced tongue carcinomas in the rat","authors":"Jun-ichi Tanuma , Motoo Kitano , Hayase Shisa , Hiroshi Hiai","doi":"10.1016/S0939-8600(00)80034-6","DOIUrl":"10.1016/S0939-8600(00)80034-6","url":null,"abstract":"<div>Oral administration of 4-nitroquinoline 1-oxide (4NQO) to rats induced a high incidence of tongue carcinomas (TCs). The inbred Dark-Agouti (DA) strain of rats showed much higher susceptibility to 4NQO-induced TCs than the Wistar-Furth (WF) strain. Our previous study on crosses between the two strains postulated a semidominant susceptibility gene in DA and a semidominant resistance gene in WF rats. This hypothesis was confirmed by the genetic analysis of the back-crosses to either parent with PCR-based microsatellite assay. Using the number of TCS with >5 mm diameter as a quantitative parameter, we mapped a quantitative trait locus Stc1 (Susceptibility to TC) favouring TC development near the locus D19Mit9 on Chr. 19 with a peak LOD score of 6.08. Two other regions in Chr. 3 and Chr. 14 showed weak linkage for susceptibility, but were not statistically significant. On the other hand, another quantitative trait locus Rtc1 (Resistance to TC) providing resistance to TCs was mapped on Chr. 1 between the loci of D1Mit1 and D1Mit3 with a peak LOD score of 3.30. Quantitative parameters such as the number of tumours in the tongue or upper alimentary tract, the frequency of larger tumours and their maximum size were closely correlated and principally determined by Stc1 and Rtc1. Therefore the susceptibility to 4NQO-induced TCs in crosses between DA and WF is explained by the combinations of genotypes at these two loci. Possible candidate genes for Stc1 and Rtc1 are discussed.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 1","pages":"Pages 68-77"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80034-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Efficiency of intrathymic tolerance induction in various inbred rat strains: relationship with TH1/TH2 status of the recipient? 不同近交系大鼠胸腺内耐受诱导效率:与受体TH1/TH2状态的关系?

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80036-X

Hans-Peter Raué, Flip Klatter, Machteld Hylkema, Margaretha van der Deen, Herman Groen, Jennie Pater, Paul Nieuwenhuis, Auk Hardonk-Wubbena, Jan-Luuk Hillebrands, Jan Rozing

{"title":"Efficiency of intrathymic tolerance induction in various inbred rat strains: relationship with TH1/TH2 status of the recipient?","authors":"Hans-Peter Raué, Flip Klatter, Machteld Hylkema, Margaretha van der Deen, Herman Groen, Jennie Pater, Paul Nieuwenhuis, Auk Hardonk-Wubbena, Jan-Luuk Hillebrands, Jan Rozing","doi":"10.1016/S0939-8600(00)80036-X","DOIUrl":"10.1016/S0939-8600(00)80036-X","url":null,"abstract":"<div>The simultaneous transplantation and intrathymic tolerance induction (STITTI) protocol induces a longlasting state of functional tolerance in over 90% of AO (RT1u) recipients transplanted with a fully MHC-incompatible PVG (RT1c) cardiac allograft. Similar results are obtained when using LEWIS (RT11) rats as recipients of either PVG or DA (RT1avl) grafts. However, when STITTI is performed on PVG and BN (RT1n) as recipient animals receiving spleen cells intrathymically and a cardiac allograft from respectively AO and PVG rats, this procedure results in significantly shorter graft survival (MST PVG → BN 25 ± 9 days; AO → PVG 31 ± 8 days) as compared to the combinations using AO (MST PVG → AO > 236 ± 28 days) and LEWIS (MST PVG → LEW > 366 ± 51 days; DA → LEW > 123 ± 33 days) rats as recipients. Since both PVG and BN rats are relatively deficient in their ability to produce IFNγ and intrathymic IFNγ responses are very dominant upon intrathymic injection of alloantigens, it is argued that the inability to effectively induce a longlasting state of functional tolerance in BN and PVG rats using the STITTI protocol may be related to their decreased IFNγ-production potential.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"41 1","pages":"Pages 82-86"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0939-8600(00)80036-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56857359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

First announcement 第一次公告

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80040-1

引用次数: 0

The rat genetic and cytogenetic maps 大鼠遗传图谱和细胞遗传学图谱

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80025-5

Claude Szpirer , Josiane Szpirer , Pascale Vanvooren , Fadel Tissir , Johanna Kela , Francoise Lallemand , Barbara Hoebee , Jason S. Simon , George Koike , Howard J. Jacob , Eric S. Lander , Khalil Helou , Karin Klinga-Levan , Göran Levan

引用次数: 0

Determination of the mouse homologous region for the rat dmy locus 大鼠基因座小鼠同源区测定

Journal of experimental animal science Pub Date : 2000-03-01 DOI: 10.1016/S0939-8600(00)80026-7

Kazuhiro Kitada , Jean-Louis Guénet , Tadao Serikawa

引用次数: 2