Analytical and quantitative cytology and histology最新文献_第4页

Discrimination between benign and malignant prostate biopsies using three-dimensional chromatin texture analysis by confocal laser scanning microscopy. 用共聚焦激光扫描显微镜三维染色质织构分析前列腺活检良恶性鉴别。

Analytical and quantitative cytology and histology Pub Date : 2011-10-01

André Huisman, Lennert S Ploeger, Hub F J Dullens, Trudy N Jonges, Paul J van Diest

{"title":"Discrimination between benign and malignant prostate biopsies using three-dimensional chromatin texture analysis by confocal laser scanning microscopy.","authors":"André Huisman, Lennert S Ploeger, Hub F J Dullens, Trudy N Jonges, Paul J van Diest","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To evaluate the clinical usefulness of computing three-dimensional (3-D) nuclear texture features on prostate biopsy specimens to discriminate among benign, prostatic intraepithelial neoplasia (PIN), and malignant specimens.Study design: Twelve prostate cancer biopsy specimens were selected, diagnosed as either benign (N = 4), PIN (N = 4), or malignant (N = 4). Sections 14 microm thick were stained. 3-D image stacks of selected benign and malignant areas were obtained by confocal laser scanning microscopy (CLSM) and analyzed off-line using in-house-developed software for 3-D semiautomated segmentation and calculation of texture features. The power of the 3-D texture features to discriminate among the pooled benign (N = 1,507), PIN (N = 673), and malignant nuclei (N = 1,251) was established by multivariate linear discriminant analysis.Results: A total of 68.8% of the benign nuclei, 77.2% of the PIN nuclei, and 78.5% of the malignant nuclei could be classified correctly after cross validation.Conclusion: Quantification of changes in the distribution of nuclear chromatin by means of 3-D texture feature computation on CLSM images allows discriminating most benign and malignant prostate nuclei, which could be useful in cases that are difficult to diagnose morphologically.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 5","pages":"265-70"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30631558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nasopharyngeal nonkeratinizing (undifferentiated) monstrous cell carcinoma. A case report. 鼻咽部非角化(未分化)畸形细胞癌。一份病例报告。

Analytical and quantitative cytology and histology Pub Date : 2011-10-01

Arzu Avci, Omer Günhan, Yildirim Karslioğlu, Bulent Celasun

引用次数: 0

Differential diagnosis of follicular cancer and follicular adenoma using an expert system based on a set of qualitative signs of cell atypia. 基于一组细胞异型性定性标志的专家系统对滤泡癌和滤泡腺瘤的鉴别诊断。

Analytical and quantitative cytology and histology Pub Date : 2011-10-01

Vladimir Kirillov, Olga Emeliyanova

{"title":"Differential diagnosis of follicular cancer and follicular adenoma using an expert system based on a set of qualitative signs of cell atypia.","authors":"Vladimir Kirillov, Olga Emeliyanova","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To determine the efficiency of the developed expert system based on a set of qualitative signs of cell atypia and their weighting coefficients in the differential diagnosis of follicular cancer and follicular adenoma of the thyroid gland.Study design: Sixty cytologic preparations of patients with histologic diagnosis of a follicular cancer and follicular adenoma were investigated.Results: Weighting coefficients for each sign of atypia for both forms of pathology have been calculated with the help of the deduced equation. This allowed creating an expert system by which the function of transforming qualitative signs of cell atypia to a quantitative form was realized. \"Strength reserve\" according to the diagnostic index value, coincidence of the verified diagnosis with the histologic conclusion, and its invariability for all 12 iterations testified to the reliability of an expert system. Preliminary trials showed the efficiency of an expert system for differentiating the nature of a thyroid follicular tumor to be 97.5%.Conclusion: The developed expert system allows high efficiency in making a differential diagnosis of thyroid follicular cancer and follicular adenoma.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 5","pages":"253-64"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30631557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined expression of p161NK4a and p27Kip1, but not p21WAF1, differentiates endocervical from endometrial adenocarcinoma. 联合表达p161NK4a和p27Kip1，但不表达p21WAF1，可区分宫颈内膜癌和子宫内膜腺癌。

Analytical and quantitative cytology and histology Pub Date : 2011-10-01

Farveen Marican Abu Backer, Nik Raihan Nik Mustapha, Nor Hayati Othman

{"title":"Combined expression of p161NK4a and p27Kip1, but not p21WAF1, differentiates endocervical from endometrial adenocarcinoma.","authors":"Farveen Marican Abu Backer, Nik Raihan Nik Mustapha, Nor Hayati Othman","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To differentiate endocervical adenocarcinoma (ECA) from endometrial adenocarcinoma (EMA) using p16INK4a, p21WAF1 and p27Kip1.Study design: The distinction between ECA and EMA can be problematic on small biopsies or in advanced tumor stage. Immunohistochemistry was done on paraffin-embedded tissue sections of hysterectomy specimens of all types of ECA and EMA. Protein expression was scored as 0 to 3 depending on percentage of positive tumor cells.Results: A total of 40 ECA and 92 EMA cases were examined. For ECA, 32 of 40 (80.0%) cases had score of 3 with p16INK4a expressions; 23 of 92 (25.0%) cases of EMA had similar scores. For p21WAF1 expressions, 28 of 40 (70.0%) ECA cases scored 3 and 72 of 82 (78.2%) of EMA had a similar score. p27Kip1 scored 3 in 17 of 40 (42.5%) ECA cases; only 14 of 92 (15.2%) cases of EMA cases had such a score. We observed significant higher expressions of p16INK4a (p < 0.001) and p27Kip1 (p = 0.001) in ECA than in EMA. Combined positive expression and combined negative expression of p16INK4a and p27Kip1 strongly differentiates ECA from EMA (< 0.0001).Conclusion: ECA could be strongly differentiated from EMA based on the expressions of combined p16INK4a and p27Kip1.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 5","pages":"283-8"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30631561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytogenetic abnormalities in exfoliated oral mucosal cells and their association with oral cancer. 脱落的口腔粘膜细胞的细胞遗传学异常及其与口腔癌的关系。

Analytical and quantitative cytology and histology Pub Date : 2011-10-01

Ana Carolina Amorim Pellicioli, Fernanda Visioli, Luciana Adolfo Ferreira, Chris Krebs Danilevicz, Vinicius Coelho Carrard, Pantelis Varvaki Rados

{"title":"Cytogenetic abnormalities in exfoliated oral mucosal cells and their association with oral cancer.","authors":"Ana Carolina Amorim Pellicioli, Fernanda Visioli, Luciana Adolfo Ferreira, Chris Krebs Danilevicz, Vinicius Coelho Carrard, Pantelis Varvaki Rados","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To assess cytogenetic abnormalities through quantification of micronuclei, broken eggs, and karyorrhexis, in cells from normal oral mucosa of individuals exposed to carcinogens (alcohol and tobacco) and adjacent to leukoplakias and squamous cell carcinomas.Study design: The sample was composed of 40 subjects aged > 30 years, divided into four groups: control, alcohol/tobacco, leukoplakia, and squamous cell carcinoma. For control and alcohol/tobacco groups, cells were collected from lower lip, tongue border, and floor of the mouth. For leukoplakia and squamous cell carcinoma groups, mucosa contralateral and adjacent to the lesions were analyzed. Cytologic smears were stained with the Feulgen reaction. A blind observer analyzed 1,000 cells per slide to quantify micronuclei, broken eggs, and karyorrhexis.Results: The leukoplakia group showed an increased number of micronuclei compared to controls (p = 0.0016) and the alcohol/tobacco group (p = 0.0048) and also increased broken eggs compared to the alcohol/tobacco group (p = 0.0172). Similarly, the carcinoma group presented more micronuclei compared to controls (p = 0.0462) and more broken eggs compared to the alcohol/tobacco group (p = 0.0104).Conclusion: The assessment of cytogenetic abnormalities micronuclei and broken eggs may be useful for monitoring individuals exposed to risk factors for developing oral cancer.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 5","pages":"271-6"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30631559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of MUC1 (Ma695) in noninvasive papillary urothelial neoplasm according to the 2004 World Health Organization classification of the noninvasive urothelial neoplasm. An immunologic tool for the pathologist? 根据2004年世界卫生组织对非侵袭性尿路上皮肿瘤的分类MUC1 (Ma695)在非侵袭性乳头状尿路上皮肿瘤中的表达。病理学家的免疫工具?

Analytical and quantitative cytology and histology Pub Date : 2011-10-01

Christian Garbar, Corinne Mascaux

{"title":"Expression of MUC1 (Ma695) in noninvasive papillary urothelial neoplasm according to the 2004 World Health Organization classification of the noninvasive urothelial neoplasm. An immunologic tool for the pathologist?","authors":"Christian Garbar, Corinne Mascaux","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To examine the association between MUC1 (Ma695) and noninvasive papillary urothelial neoplasm according to the 2004 World Health Organization classification.Study design: Histologic evaluation was performed in 46 patients with nontumoral benign bladder urothelium (n = 11), papillary urothelial neoplasm of low malignant potential (PUNLMP) (n = 14), low-grade papillary urothelial carcinoma (LgPUC) (n = 11), and noninvasive high-grade papillary urothelial carcinoma (HgPUC) (n = 10). Classical morphologic description and semiquantitative digital measurement were performed from immunohistochemical-stained slides using an anti-MUC1 antibody (Ma695).Results: Measures showed an obvious statistical difference between benign urothelium and LgPUC (p = 0.0004) or HgPUC (p = 0.0041). MUC1 is expressed less in PUNLMP than in LgPUC (p = 0.04). Benign urothelium and PUNLMP more often showed apical and superficial MUC1 expression. Basal cells with cytoplasmic and/or circumferential membrane positivity were more often observed in LgPUC and HgPUC (p < 0.001).Conclusion: MUC1 could be an interesting tool for the pathologist to differentiate between PUNLMP and LgPUC or HgPUC.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 5","pages":"277-82"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30631560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic therapy in cancer: perspective and paradoxes. 癌症的表观遗传治疗:观点和悖论。

Analytical and quantitative cytology and histology Pub Date : 2011-10-01

Alessandra Filosa, Andrea Fabiani

引用次数: 0

A decision support system for assisting fine needle aspiration diagnosis of thyroid malignancy. 辅助甲状腺恶性肿瘤细针穿刺诊断的决策支持系统。

Analytical and quantitative cytology and histology Pub Date : 2011-08-01

Emmanouil A Zoulias, Pantelis A Asvestas, George K Matsopoulos, Sofia Tseleni-Balafouta

{"title":"A decision support system for assisting fine needle aspiration diagnosis of thyroid malignancy.","authors":"Emmanouil A Zoulias, Pantelis A Asvestas, George K Matsopoulos, Sofia Tseleni-Balafouta","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To assist diagnosis of thyroid malignancy, implementing a decision support system (DSS) using fine needle aspiration biopsy (FNAB) data.Study design: The set of 2,035 thyroid smears contained 1,886 smears of nonmalignancy (class 1) and 150 smears of malignancy (class 2) verified histologically. For each smear, 67 medical features were considered by the expert, forming 2,036 feature vectors, which were fed into a DSS for discriminating between malignant and nonmalignant smears. The DSS comprised a feature selection and classification module using a combination of three classifiers, the artificial neural network, the support vector machines, and the k-nearest neighbor, under the majority vote procedure.Results: The overall classification accuracy of the DSS was 98.6%, marginally better than the FNAB (97.3%). The DSS had lower sensitivity (89.1%) and better specificity (99.4%) compared to the FNAB. Regarding the smears characterized as \"suspicious\" by FNAB, a significant improvement of overall accuracy was obtained by the proposed DSS system (84.6%) compared to the FNAB (50.0%).Conclusion: The proposed DSS provides significant improvement compared to FNAB regarding discrimination of smears characterized by an expert as \"suspicious,\" reducing the number of patients undergoing surgical procedures.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 4","pages":"215-22"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30191914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear morphology for the detection of alterations in bronchial cells from lung cancer: an attempt to improve sensitivity and specificity. 肺癌支气管细胞改变的核形态学检测:提高敏感性和特异性的尝试。

Analytical and quantitative cytology and histology Pub Date : 2011-08-01

Mélanie Fafin-Lefevre, Fabrice Morlais, Lydia Guittet, Bénédicte Clin, Guy Launoy, Françoise Galateau-Sallé, Benoît Plancoulaine, Paulette Herlin, Marc Letourneux

{"title":"Nuclear morphology for the detection of alterations in bronchial cells from lung cancer: an attempt to improve sensitivity and specificity.","authors":"Mélanie Fafin-Lefevre, Fabrice Morlais, Lydia Guittet, Bénédicte Clin, Guy Launoy, Françoise Galateau-Sallé, Benoît Plancoulaine, Paulette Herlin, Marc Letourneux","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To identify which morphologic or densitometric parameters are modified in cell nuclei from bronchopulmonary cancer based on 18 parameters involving shape, intensity, chromatin, texture, and DNA content and develop a bronchopulmonary cancer screening method relying on analysis of sputum sample cell nuclei.Study design: A total of 25 sputum samples from controls and 22 bronchial aspiration samples from patients presenting with bronchopulmonary cancer who were professionally exposed to cancer were used. After Feulgen staining, 18 morphologic and DNA content parameters were measured on cell nuclei, via image cytom- etry. A method was developed for analyzing distribution quantiles, compared with simply interpreting mean values, to characterize morphologic modifications in cell nuclei.Results: Distribution analysis of parameters enabled us to distinguish 13 of 18 parameters that demonstrated significant differences between controls and cancer cases. These parameters, used alone, enabled us to distinguish two population types, with both sensitivity and specificity > 70%. Three parameters offered 100% sensitivity and specificity. When mean values offered high sensitivity and specificity, comparable or higher sensitivity and specificity values were observed for at least one of the corresponding quantiles.Conclusion: Analysis of modification in morphologic parameters via distribution analysis proved promising for screening bronchopulmonary cancer from sputum.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 4","pages":"183-95"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30191910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidermal growth factor receptor status in stages of resected non-small cell lung cancer: implications for treatment with epidermal growth factor receptor inhibitors. 表皮生长因子受体在切除的非小细胞肺癌分期中的状态:表皮生长因子受体抑制剂治疗的意义。

Analytical and quantitative cytology and histology Pub Date : 2011-08-01

Rossana Berardi, Alfredo Santinelli, Alessandro Brunelli, Azzurra Onofri, Chiara Pierantoni, Mario Scartozzi, Eleonora Pisa, Daniela Stramazzotti, Paola Mazzanti, Lina Zuccatosta, Stefano Gasparini, Armando Sabbatini, Stefano Cascinu

{"title":"Epidermal growth factor receptor status in stages of resected non-small cell lung cancer: implications for treatment with epidermal growth factor receptor inhibitors.","authors":"Rossana Berardi, Alfredo Santinelli, Alessandro Brunelli, Azzurra Onofri, Chiara Pierantoni, Mario Scartozzi, Eleonora Pisa, Daniela Stramazzotti, Paola Mazzanti, Lina Zuccatosta, Stefano Gasparini, Armando Sabbatini, Stefano Cascinu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To verify whether epidermal growth factor receptor (EGFR) status could be considered a prognostic factor and assessment of it an effective tool for planning therapy in patients with non-small cell lung cancer (NSCLC).Study design: From 1996 to 2001, 439 patients underwent radical surgery for NSCLC at the Polytechnic University of the Marche Region. EGFR expression was detected as membranous and/or cytoplasmic staining of neoplastic cells with various intensity and was considered positive when > or = 1% of the tumor cells had membranous staining.Results: Samples from 423 patients were available for EGFR analysis. EGFR expression and a stronger intensity of staining were associated with a trend for a worse prognosis in the analysis of all of the patients. The subgroup analysis showed no prognostic significance in stages I and II but a significantly longer survival in patients with advanced disease (stage III and particularly N2) overexpressing EGFR.Conclusion: The results of our study, showing a significantly longer survival in patients with advanced disease (stage III, particularly N2) overexpressing EGFR, present a new perspective, both for prognostic evaluation of patients with radically resected NSCLC and for the management of adjuvant treatment also employing targeted therapy.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 4","pages":"196-204"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30191911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0