Analytical and quantitative cytology and histology最新文献_第3页

Evaluation of peritoneal fluid hemosiderin-laden macrophages in biopsy-proven endometriosis. 活检证实的子宫内膜异位症腹膜液含铁血黄素巨噬细胞的评价。

Analytical and quantitative cytology and histology Pub Date : 2012-02-01

Mohamed Ali Bedaiwy, Javier Noriega, Mahmoud Abdel Aleem, Sajal Gupta, Ahmed M AbulHassan, Jennifer Brainard, Alaa M Ismail, Tommaso Falcone

{"title":"Evaluation of peritoneal fluid hemosiderin-laden macrophages in biopsy-proven endometriosis.","authors":"Mohamed Ali Bedaiwy, Javier Noriega, Mahmoud Abdel Aleem, Sajal Gupta, Ahmed M AbulHassan, Jennifer Brainard, Alaa M Ismail, Tommaso Falcone","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To evaluate peritoneal fluid hemosiderin-laden macrophages (H-LMs) in patients with endometriosis compared to controls.Study design: Consecutive series of 46 patients during a year undergoing laparoscopy for benign gynecologic conditions were included. The presence of H-LMs in peritoneal fluid was evaluated. We compared clinical factors in patients with or without endometriosis in respect to H-LM status. To assess the potential of H-LMs to diagnose endometriosis, the sensitivity and specificity were calculated.Results: Patients with endometriosis were significantly more likely to have positive H-LM test results than controls (p = 0.0013). The presence of H-LM has a low sensitivity of 52% but an acceptable specificity of 87% in diagnosing endometriosis. The presence of H-LMs was not related with any other of the clinical factors studied.Conclusion: The presence of specific findings of H-LMs related to endometriosis strongly suggests abnormalities in peritoneal iron metabolism.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"34 1","pages":"23-7"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30621928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aberrant connexin 43 and 26 expression in cervical dysplasia. 连接蛋白43和26在宫颈发育不良中的异常表达。

Analytical and quantitative cytology and histology Pub Date : 2012-02-01

Ian S Hagemann, Theresa L Pasha, Shelley A Roberts, Sabrina W Yum, Paul J Zhang

{"title":"Aberrant connexin 43 and 26 expression in cervical dysplasia.","authors":"Ian S Hagemann, Theresa L Pasha, Shelley A Roberts, Sabrina W Yum, Paul J Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To determine whether connexin (Cx) expression is altered in cervical dysplasia.Study design: Cx proteins form gap junctions and are expressed in squamous epithelia including ectocervix. We used multispectral imaging to perform a quantitative immunohistochemical survey of Cx43 Cx26 in 37 archival human cervical specimens.Results: Cx43 expression was very low in normal cervix (100%), but was increased in low-grade squamous intraepithelial lesions (LSILs) (64%), primarily in a parabasal distribution. High-grade squamous intraepithelial lesions (HSILs) showed weak full-thickness Cx43 staining (53%) or lacked Cx43 (47%). An aberrant increase in Cx43 expression was often (62%) present in histologically normal areas of specimens that elsewhere harbored dysplasia. Cx26 was highly expressed in the basal layer of normal ectocervix (100%). In LSIL, 57% showed a decrease in Cx26 and the rest showed no change relative to the normal pattern. In HSIL, Cx26 was expressed in the full thickness of the epithelium, at a high level in 80% of cases and a low level in the rest.Conclusion: Cx alteration is moderately consistent in cervical dysplasia, and for Cx43 can precede histologic changes. The resulting changes in Cx signaling may be important in the pathogenesis of cervical intraepithelial neoplasia.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"34 1","pages":"28-40"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30621929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cascaded learning vector quantizer neural networks for the discrimination of thyroid lesions. 用于甲状腺病变识别的级联学习向量量化神经网络。

Analytical and quantitative cytology and histology Pub Date : 2011-12-01

Alexandra Varlatzidou, Abraham Pouliakis, Magdalini Stamataki, Christos Meristoudis, Niki Margari, George Peros, John G Panayiotides, Petros Karakitsos

{"title":"Cascaded learning vector quantizer neural networks for the discrimination of thyroid lesions.","authors":"Alexandra Varlatzidou, Abraham Pouliakis, Magdalini Stamataki, Christos Meristoudis, Niki Margari, George Peros, John G Panayiotides, Petros Karakitsos","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate capability of combination of learning vector quantizer (LVQ) neural networks (NNs) in discrimination of benign from malignant thyroid lesions.Study design: The study included 335 liquid-based cytology, fine needle aspiration (FNA), Papanicolaou-stained specimens. All cases were compared to the histologic diagnosis. Features describing size, shape, and texture of -100 nuclei per case were extracted from cytologic images using a custom image analysis system. These features were used to classify each nucleus by LVQ type NNs. The nucleus classification results were used to classify individual lesions with a second LVQ NN. Cases were distributed by histologic diagnosis. Data from -50% from each category were used for training LVQ classifiers. Remaining data were used to test classifier performance. The system was used to discriminate to individual cellular level and individual patient level between benign and malignant nuclei.Results: Application of the proposed algorithm combining two LVQ NNs allows discrimination between benign and malignant cell nuclei and lesions.Conclusion: Results indicate that use of NNs, combined with image morphometry, can provide information on thyroid lesion malignancy potential. The system could improve FNA diagnostic accuracy of the thyroid gland, especially in follicular neoplasms suspicious for malignancy and in Hürthle cell tumors.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 6","pages":"323-34"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30621407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of an artificial neural network in the prognosis of chronic myeloid leukemia. 人工神经网络在慢性髓性白血病预后中的应用。

Analytical and quantitative cytology and histology Pub Date : 2011-12-01

Pranab Dey, Amit Lamba, Savita Kumari, Neelam Marwaha

{"title":"Application of an artificial neural network in the prognosis of chronic myeloid leukemia.","authors":"Pranab Dey, Amit Lamba, Savita Kumari, Neelam Marwaha","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To use a commercially available artificial neural network (ANN) software program to distinguish prognostically good and bad cases of chronic myeloid leukemia (CML).Study design: A total of 40 patients with CML who developed blast crisis or proceeded in the accelerated phase were selected. They formed two groups, group 1 and group 2, of 20 patients each, who developed accelerated phase or blast crisis within 18 months or 30 months, respectively, after the initial diagnosis of the chronic phase of CML. The detailed clinical, hematologic, and morphometric data were collected in all these cases. A suitable ANN software program was used to analyze these data.Results: All cases were randomly distributed automatically by the program into three groups: training set (28), validation set (4), and test set (8). In the test set, the ANN program successfully classified all group I and group II patients.Conclusion: We successfully used a commercially available ANN software program to develop a model able to classify prognostically good and bad cases of CML.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 6","pages":"335-9"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30621923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pelvic urothelial carcinoma with nested pattern of growth and an uncommon clinical presentation: a case report. 盆腔尿路上皮癌巢状生长和罕见的临床表现:1例报告。

Analytical and quantitative cytology and histology Pub Date : 2011-12-01

Sergio Tripodi, Bruno Jim Rocca, Maria Raffaella Ambrosio, Filippo Gentile, Marcella Cintorino

{"title":"Pelvic urothelial carcinoma with nested pattern of growth and an uncommon clinical presentation: a case report.","authors":"Sergio Tripodi, Bruno Jim Rocca, Maria Raffaella Ambrosio, Filippo Gentile, Marcella Cintorino","doi":"","DOIUrl":"","url":null,"abstract":"Background: Nested variant of urothelial carcinoma (NVUC) is a rare and often unrecognized urothelial neoplasia. Diagnosis is based on morphology only, and no immunohistochemical or cytogenetic differences from usual high-grade urothelial carcinomas have been reported.Case: We describe the case of a 49-year-old woman affected by hepatitis C virus presented with fever, discomfort, urgency, and hypertension. Computed tomography showed a sclerosing inflammatory process involving the connective and adipose tissue of the renal sinus. In the absence of renal or pelvic masses an underlying malignancy was excluded and renal abscess or tuberculosis was suspected. Accordingly, nephrectomy and proximal ureterectomy was performed. Grossly, calices, renal pelvis, and pyeloureteral junction appeared modestly dilated with whitish, thickened, and uneven mucosa. Microscopically, the subepithelial connective tissue, the fibromuscular layer, and the renal sinus fat were diffusely infiltrated by small nests of medium to large urothelial cells (p63 positive) with abundant eosinophylic cytoplasm and slightly atypical nuclei.Conclusion: On the basis of morphologic and immunohistochemical features, a diagnosis of NVUC was made. After surgery, the patient recovered from hypertension. Pelvic and upper urothelial tract NVUCs are uncommon, and to the best of our knowledge, this is the second case of NVUC with renal involvement.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 6","pages":"340-4"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30621924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical performance of human papillomavirus E6, E7 mRNA flow cytometric assay compared to human papillomavirus DNA typing. 临床表现人乳头瘤病毒E6、E7 mRNA流式细胞测定比较人乳头瘤病毒DNA分型。

Analytical and quantitative cytology and histology Pub Date : 2011-12-01

Christine Kottaridi, Sotirios Tsiodras, Aris Spathis, Aikaterini Chranioti, Asimakis Pappas, Dimitrios Kassanos, Ioannis Panayiotides, Petros Karakitsos

{"title":"Clinical performance of human papillomavirus E6, E7 mRNA flow cytometric assay compared to human papillomavirus DNA typing.","authors":"Christine Kottaridi, Sotirios Tsiodras, Aris Spathis, Aikaterini Chranioti, Asimakis Pappas, Dimitrios Kassanos, Ioannis Panayiotides, Petros Karakitsos","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To use flow cytometry to screen cervical samples for the overexpression of human papillomavirus (HPV) E6 and E7 mRNA and compare the performance of this assay with an HPV DNA array for the detection of high-grade cervical lesions.Study design: Cervical samples were analyzed for HPV DNA by clinical arrays, and the overexpression of E6 and E7 viral oncogenes was monitored using an HPV mRNA detection kit that quantifies the intracellular HPV E6 and E7 mRNA on a cell-by-cell basis.Results: HPV positivity increased with severity of histologic lesions. On the basis of histology-confirmed CIN 2+ cases the specificity of HPV assay was 73.9% (95% CI 66.07, 80.88), whereas it was 39.3% (95% CI 31.85, 47.1) for the DNA assay.Conclusion: The HPV assay provides an early predictor of persistent HPV infection and may improve cervical cancer screening by increasing the specificity of detecting high-grade lesions.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 6","pages":"305-10"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30621404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human papillomavirus E6/E7 mRNA testing has higher specificity than liquid-based DNA testing in the evaluation of cervical intraepithelial neoplasia. 人乳头瘤病毒E6/E7 mRNA检测在宫颈上皮内瘤变评价中比液体DNA检测具有更高的特异性。

Analytical and quantitative cytology and histology Pub Date : 2011-12-01

Jochen Möckel, Jens Quaas, Helga Meisel, Anne-Sophie Endres, Volker Schneider

{"title":"Human papillomavirus E6/E7 mRNA testing has higher specificity than liquid-based DNA testing in the evaluation of cervical intraepithelial neoplasia.","authors":"Jochen Möckel, Jens Quaas, Helga Meisel, Anne-Sophie Endres, Volker Schneider","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To examine the specificity of human papillomavirus (HPV) E6/E7 mRNA testing for intraepithelial precursor lesions and invasive carcinoma of the uterine cervix in 358 women and compare the results with those of the most widely used DNA technique.Study design: For HPV E6/E7 mRNA testing an amplification assay was used. For DNA determination a hybridization assay was applied. Both techniques were used simultaneously in patients with normal morphology (150), cervical intraepithelial neoplasia (173) and invasive carcinoma of the cervix (35).Results: HPV DNA positivity rates were significantly higher than E6/E7 mRNA in women with normal morphology (21-7%), cervical intraepithelial neoplasia (CIN) 1 and 2 (75-43%), and CIN 3 (93-63%). In invasive cervical carcinoma, both methods tested equally high (94% vs. 97%). Considering that E6/E7 up-regulation represents the initial step in cervical carcinogenesis, it can be assumed that this test allows a more specific detection of lesions with a potential for progression.Conclusion: HPV E6/E7 mRNA may serve as a more specific discriminator between transient cervical dysplasias and potentially progressive lesions. Accordingly, testing for high-risk HPV E6/E7 mRNA might reduce the psychologic burden associated with HPV-DNA testing.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 6","pages":"311-5"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30621405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular findings of nasopharyngeal carcinoma in a European population. 欧洲人群鼻咽癌的分子研究结果。

Analytical and quantitative cytology and histology Pub Date : 2011-12-01

María Teresa González Serrano, María José Ríos Moreno, Manuel de Miguel Rodríguez, Fernando Casco, Hugo Galera-Ruiz, María Teresa Vargas de los Monteros, Inmaculada Trigo Sánchez, Ricardo González-Cámpora, Hugo Galera-Davidson

{"title":"Molecular findings of nasopharyngeal carcinoma in a European population.","authors":"María Teresa González Serrano, María José Ríos Moreno, Manuel de Miguel Rodríguez, Fernando Casco, Hugo Galera-Ruiz, María Teresa Vargas de los Monteros, Inmaculada Trigo Sánchez, Ricardo González-Cámpora, Hugo Galera-Davidson","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To explore biomolecular characteristics of a group of patients with nasopharyngeal carcinoma from European (Spanish) hospitals, addressing the pathogenesis of the tumor and the response to treatment.Study design: Cyclin D1 and p16 expression were evaluated immunohistochemically in 33 tissue samples of nasopharyngeal carcinoma. CCDN1 gene amplification and p16 gene deletion were studied by fluorescence in situ hybridization. Patient clinical data were examined, and tissues were evaluated histologically using hematoxylin-eosin staining.Results: Cyclin D1 overexpression was found in 19 cases, and p16 expression was undetected in 30 cases. An association was observed between impaired p16 expression and cyclin D1 overexpression (p = 0.034). Eleven patients displayed p16 gene deletion and CCDN1 gene amplification.Conclusion: Cyclin D1 overexpression and CCDN1 amplification, loss of p16 expression and p16 deletion may be among the genetic alterations involved in the pathogenesis of nasopharyngeal carcinoma.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 6","pages":"316-22"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30621406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative microscopy in murine models of lung inflammation. 小鼠肺部炎症模型的定量显微镜观察。

Analytical and quantitative cytology and histology Pub Date : 2011-10-01

Joseph Soltzberg, Sarah Frischmann, Christiaan van Heeckeren, Nicole Brown, Arnold Caplan, Tracey L Bonfield

{"title":"Quantitative microscopy in murine models of lung inflammation.","authors":"Joseph Soltzberg, Sarah Frischmann, Christiaan van Heeckeren, Nicole Brown, Arnold Caplan, Tracey L Bonfield","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To develop a quantitative means to measure lung inflammation using the murine models of chronic asthma and cystic fibrosis (CF).Study design: Translational-based medicine often utilizes animal models to study new and innovative therapeutics. In asthma and CF, the animal models focus on airway inflammation and remodeling. The asthma model is based on hypersensitivity-induced airway disease, whereas the CF model focuses on the inflammatory response to infection with Pseudomonas aeruginosa. Qualitative measures of inflammation and lung pathophysiology introduce significant variability and difficulty in interpreting interventional outcomes. The highly sensitive and reproducible quantitative computational program interfaced with Image Pro Microscopy to monitor changes in lung inflammation and lung pathophysiology. The software interfaces with image microscopy and automates the lung section review process.Results: Results from this program recapitulated data obtained by manual point counting of inflammation, bronchoalveolar lavage differential, and histology. The data show a low coefficient of variation and high reproducibility between slides and sections.Conclusion: Utilization of this new microscopy program will enhance the quantitative means of establishing changes in lung structure and inflammation as a measure of therapeutic intervention with the ability of refining interpretation of in vivo models potentially short-circuiting translation into the clinical setting.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 5","pages":"245-52"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4195243/pdf/nihms-627926.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30631556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple flow cytometric protocol of CD4+/CD8+ lymphocyte ratio assessment in bronchoalveolar lavage fluids from patients with interstitial lung diseases. 肺间质性疾病患者支气管肺泡灌洗液CD4+/CD8+淋巴细胞比值的简单流式细胞术评估

Analytical and quantitative cytology and histology Pub Date : 2011-10-01

Adam Szpechcinski, Piotr Kopinski, Dorota Giedronowicz, Adriana Rozy, Paulina Jagus, Malgorzata Szolkowska, Joanna Chorostowska-Wynimko

{"title":"Simple flow cytometric protocol of CD4+/CD8+ lymphocyte ratio assessment in bronchoalveolar lavage fluids from patients with interstitial lung diseases.","authors":"Adam Szpechcinski, Piotr Kopinski, Dorota Giedronowicz, Adriana Rozy, Paulina Jagus, Malgorzata Szolkowska, Joanna Chorostowska-Wynimko","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To validate the fast and accurate flow cytometric (FCM) protocol using blood-standardized antibodies for alveolar lymphocyte subtyping with respect to standard immunocytochemistry (IC).Study design: FCM and IC were applied to immunophenotype T cell subsets in bronchoalveolar lavage (BAL) fluids from patients with interstitial lung diseases. Diagnostic BAL specimens from 50 patients with suspected sarcoidosis, idiopathic pulmonary fibrosis, and hypersensitivity pneumonitis were evaluated by both IC and FCM. In FCM, CD4+ and CD8+ T cells were identified by light scatter gating with CD3 selection using basic tricolor cytometer.Results: Relative amounts of CD4+, CD8+ T cells, and CD4+/CD8+ ratios demonstrated by the FCM showed excellent, significant correlations with IC results. FCM values did not differ significantly from IC results. However, the sensitivity and specificity of conventional IC staining were not sufficient to assess CD4+/ CD8+ ratio in most idiopathic pulmonary fibrosis cases. Additionally, performing IC immunophenotyping in BAL samples with low lymphocyte content introduced a remarkable error into CD4+/CD8+ ratio assessment.Conclusion: FCM allowed reliable, precise, and fast T-cell subset measurement in all BAL samples, overcoming the IC disadvantages. Our validated FCM protocol provides diagnostically relevant CD4+/CD8+ ratio determination by simple light scatter gating strategy with CD3 selection.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 5","pages":"289-96"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30631562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0