Analytical and quantitative cytology and histology最新文献_第10页

Sorafenib's inhibition of prostate cancer growth in transgenic adenocarcinoma mouse prostate mice and its differential effects on endothelial and pericyte growth during tumor angiogenesis. 索拉非尼对转基因腺癌小鼠前列腺癌生长的抑制作用及其对肿瘤血管生成过程中内皮细胞和周细胞生长的差异影响。

Analytical and quantitative cytology and histology Pub Date : 2010-06-01

Aldo V Bono, Tania Pannellini, Marcella Liberatore, Rodolfo Montironi, Sergio Cosciani Cunico, Liang Cheng, Francesco Sasso, Piero Musiani, Manuela Iezzi

{"title":"Sorafenib's inhibition of prostate cancer growth in transgenic adenocarcinoma mouse prostate mice and its differential effects on endothelial and pericyte growth during tumor angiogenesis.","authors":"Aldo V Bono, Tania Pannellini, Marcella Liberatore, Rodolfo Montironi, Sergio Cosciani Cunico, Liang Cheng, Francesco Sasso, Piero Musiani, Manuela Iezzi","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To evaluate Sorafenib's efficacy (60 mg/kg/d per os) in preventing the transformation of high grade prostate intraepithelial neoplasia (HGPIN) into adenocarcinoma (ADC) and in inhibiting the onset and progression of poorly differentiated carcinoma (PDC) in transgenic adenocarcinoma mouse prostate (TRAMP) mice.Study design: Forty-four TRAMP mice were randomly divided into 2 groups of 22 and assigned to daily treatment by gavage with vehicle only or Sorafenib from the 10th to the 26th week of age. At 26 weeks of age the mice were killed, and their genitourinary apparatus was removed and examined by histology, immunohistochemistry and confocal microscopy.Results: Sorafenib reduced HGPIN growth and progression to ADC and was probably also effective in PDC inhibition. The major effect of Sorafenib was on tumor angiogenesis. Interestingly a dissociation between endothelial cells and pericytes was noted in treated PDC since inhibition of pericyte recruitment was less complete than that of endothelial cells.Conclusion: Sorafenib's potent antiangiogenic action may be supposed to be exerted primarily by inhibiting endothelial proliferation and sprouting, whereas its inhibition of pericyte recruitment and maturation is less complete. These observations suggest that Sorafenib's effects could be improved by the joint employment of substances capable of interfering with the recruitment and organization of pericytes.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 3","pages":"136-45"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29182067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urachal urothelial carcinoma diagnosed at a radical prostatectomy operation: a case report. 在根治性前列腺切除术中诊断的尿管尿路上皮癌1例报告。

Analytical and quantitative cytology and histology Pub Date : 2010-06-01

Nalan Nese, Gonca Kesici, Murat Lekili, Aydin Isisag

{"title":"Urachal urothelial carcinoma diagnosed at a radical prostatectomy operation: a case report.","authors":"Nalan Nese, Gonca Kesici, Murat Lekili, Aydin Isisag","doi":"","DOIUrl":"","url":null,"abstract":"Background: Urachal carcinomas are rare tumors, and the majority of them are adenocarcinomas. Up to now, only 21 urachal urothelial carcinomas (UCas) have been reported. Here, we describe a case of high grade UCa arising from the urachal remnants.Case: A 66-year-old man presented with voiding difficulties. Prostate specific antigen (PSA) was 5.46 ng/mL. Prostatic adenocarcinoma (PCa) (Gleason score 6) was diagnosed by needle biopsies. After the diagnosis of high grade, muscle invasive UCa with intact mucosa on frozen examination of the dome of bladder wall during the radical prostatectomy operation (RPO), partial cystectomy was performed. Microscopically, among the tumoral islands, cystic structures lined by cells with a benign appearance, which are considered urachal remnants, were noted. Cytokeratin 7 and high-molecular-weight cytokeratin were strongly positive, PSA and carcinoembryonic antigen were negative. Radiotherapy was given for both UCa and PCa. Six months after the diagnosis, an undifferentiated tumor was detected in a bladder transurethral resection specimen; thus, chemotherapy was given. After 1 course of chemotherapy, the patient was doing well.Conclusion: The origin of urachal carcinomas is usually obscured as it is a highly invasive carcinoma. The patient presented here was diagnosed incidentally during RPO. Although the stage was advanced, the tumor was detected before urachal remnants were destroyed. The treatment choice for urachal carcinomas is cystectomy. Adjuvant chemotherapy and radiotherapy are controversial.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 3","pages":"174-7"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29183155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ploidy status of odontogenic tumors with epithelial components. 具有上皮成分的牙源性肿瘤的倍性状况。

Analytical and quantitative cytology and histology Pub Date : 2010-06-01

Umit Karacayli, Ayper Kaya, Yavuz S Aydintug, Omer Gunhan

引用次数: 0

Comparison between incidentally and clinically detected prostate cancer: implications for prostate cancer screening programs and focal therapy. 偶然和临床检测前列腺癌的比较:前列腺癌筛查方案和局灶治疗的意义。

Analytical and quantitative cytology and histology Pub Date : 2010-06-01

Roberta Mazzucchelli, Rodolfo Montironi, Doriana Morichetti, Marina Scarpelli, Antonio Lopez-Beltran, Liang Cheng

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Quantitative analysis in canine cutaneous mast cell tumors. 犬皮肤肥大细胞瘤的定量分析。

Analytical and quantitative cytology and histology Pub Date : 2010-06-01

Radostin Simeonov, Galina Simeonova

引用次数: 0

Fecal cytology in conjunction with immunofecal occult blood test for colorectal cancer screening. 粪便细胞学与免疫粪便潜血试验联合用于结直肠癌筛查。

Analytical and quantitative cytology and histology Pub Date : 2010-06-01

Jin-qiu Sheng, Shi-rong Li, Hui Su, Jian-sheng Li, Zi-qin Sun, Zi-tao Wu, Xia Wu, Chang-hong Xia, Jianyu Rao

{"title":"Fecal cytology in conjunction with immunofecal occult blood test for colorectal cancer screening.","authors":"Jin-qiu Sheng, Shi-rong Li, Hui Su, Jian-sheng Li, Zi-qin Sun, Zi-tao Wu, Xia Wu, Chang-hong Xia, Jianyu Rao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To develop a simple method to extract and analyze the cytomorphology of epithelial cells from fecal samples and to compare the efficacy of fecal cytology with the immunofecal occult blood test (IFOBT) in colorectal cancer screening.Study design: Fecal cytology and IFOBT were performed on fecal samples obtained from 41 patients with colorectal cancer; 34 patients with a small, single adenoma (<0.5 cm); and 20 without abnormality. The samples were obtained prior to colonoscopic examination. For fecal cytology, epithelial cells were exacted through filtration, centrifugation and cytocentrifugation and stained with hematoxylin-eosin prior to morphologic analysis.Results: Fecal cytology and IFOBT test had similar levels of sensitivity for detecting colorectal cancer (75.6% vs. 68.3%, respectively), but fecal cytology had higher specificity than IFOBT (100% as compared to 85.2%, respectively, p<0.05 by chi2 test). Seven of 41 colorectal cancer patients (17.1%) with negative IFOBT were positive by fecal cytology analysis. Combining fecal cytology with the IFOBT test in an either/or scenario significantly increased the sensitivity of IFOBT test to 92.68% for colorectal cancer detection (p<0.05 by chi2 test) without compromising the specificity.Conclusion: Fecal cytology augments the sensitivity of IFOBT in detecting colorectal cancers, and combining fecal cytology and IFOBT may provide an important simple and cost-effective alternative for colon cancer screening.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 3","pages":"131-5"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29182066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary comparison of tumor biologic factors in breast carcinomas from Australian and Chinese women. 澳大利亚和中国妇女乳腺癌肿瘤生物学因素的初步比较。

Analytical and quantitative cytology and histology Pub Date : 2010-06-01

Weiqiang Zheng, Jane E Armes, John Slavin, Yuzhao Zhu, Canrong Ni

{"title":"Preliminary comparison of tumor biologic factors in breast carcinomas from Australian and Chinese women.","authors":"Weiqiang Zheng, Jane E Armes, John Slavin, Yuzhao Zhu, Canrong Ni","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To compare the morphologic and immunohistochemical properties of breast carcinomas from Chinese and Australian women in order to define possible biologic differences between these carcinomas.Study design: Three hundred cases of breast carcinomas were assessed for histologic and immunophenotypic characteristics from the pathology archives of the Changhai and St. Vincent's Hospitals.Results: The Chinese women had proportionally more grade 2 and 3 tumors, whereas Australian women had a higher proportion of grade 1 tumor. There was a higher proportion of younger patients with a larger tumor and patients with lymph node involvement in the Chinese group as compared with Australian women. There was no difference in rate of estrogen receptor positive tumors between the 2 groups. p53 Expression was statistically more common with less cyclin D1 expression in Chinese as compared with Australian women.Conclusion: This study indicates that both inherent tumor biology and stage at presentation influence adversely affect the outcome of breast carcinoma in Chinese compared as with Australian women.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 3","pages":"155-60"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29183152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Importance of flow cytometric analysis of serous effusions in the diagnosis of hematopoietic neoplasms in patients with prior hematopoietic malignancies. 浆液流式细胞分析在既往恶性造血肿瘤患者中诊断造血肿瘤的重要性。

Analytical and quantitative cytology and histology Pub Date : 2010-06-01

Jabed Iqbal, Ting Liu, Beth Mapow, Vanlila K Swami, J Steve Hou

{"title":"Importance of flow cytometric analysis of serous effusions in the diagnosis of hematopoietic neoplasms in patients with prior hematopoietic malignancies.","authors":"Jabed Iqbal, Ting Liu, Beth Mapow, Vanlila K Swami, J Steve Hou","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To determine the criteria for the use of immunophenotyping by flow cytometry (FCM) in the diagnosis of hematopoietic lesions.Study design: A retrospective review of 89 consecutive body fluid specimens with concurrent FCM analysis during 2001 to 2006 was performed. The cytopathologic diagnosis was compared with the final diagnosis as modified by subsequent FCM.Results: The cytopathologic diagnosis was benign in 61 cases (69%), atypical in 20 cases (22%) and malignant in 8 cases (9%). In patients without any prior clinical history, FCM study was positive in 2 cases and negative in 49 cases. In these patients, the working cytopathologic diagnosis was modified from benign/atypical to malignant in 2 (11%) cases and atypical to benign in 11 (33%) cases. In patients with a prior clinical history, FCM was positive in 23 cases and negative in 15 cases.Conclusion: FCM studies were helpful in the cytopathologic diagnosis in 35% of body fluid specimens, permitting appropriate cancer staging and management. In the absence of a prior clinical history, immunophenotyping by FCM in body fluid specimens should be ordered after adequacy studies when there is cytologic atypia or a strong suspicion of malignancy on the cytopathologic diagnosis.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 3","pages":"161-5"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29183153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of fluorescence in situ hybridization and hybrid capture 2 analyses of cervical cytology specimens diagnosed as low grade squamous intraepithelial lesion for the detection of high grade cervical intraepithelial neoplasia. 对诊断为低级别鳞状上皮内病变的宫颈细胞学标本进行荧光原位杂交和杂交捕获2分析，以检测高级别宫颈上皮内瘤变。

Analytical and quantitative cytology and histology Pub Date : 2010-06-01

Jesse S Voss, Benjamin R Kipp, Michael B Campion, Irina A Sokolova, Michael R Henry, Kevin C Halling, Amy C Clayton

{"title":"Assessment of fluorescence in situ hybridization and hybrid capture 2 analyses of cervical cytology specimens diagnosed as low grade squamous intraepithelial lesion for the detection of high grade cervical intraepithelial neoplasia.","authors":"Jesse S Voss, Benjamin R Kipp, Michael B Campion, Irina A Sokolova, Michael R Henry, Kevin C Halling, Amy C Clayton","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To assess Hybrid Capture 2 (HC2) and fluorescence in situ hybridization (FISH) for the detection of cervical intraepithelial neoplasia 2 or worse (CIN 2+) in patients with a cytologic diagnosis of low grade squamous intraepithelial lesion (LSIL).Study design: Residual samples from 115 LSIL-diagnosed cervical cytology specimens were evaluated by high-risk human papillomavirus (HR-HPV) HC2 testing and FISH using biotin-labeled probes to HR-HPV and chromosomal probes to 3q26 (TERC) and 8q24 (CMYC). A cervical biopsy diagnosis of CIN 2+ was considered as evidence of high grade disease.Results: The positive and negative predictive values of HC2 and FISH for detecting patients with CIN 2+ were 32% vs. 37% and 100% vs. 93%, respectively. The sensitivities of HC2 and FISH for CIN 2+ were not significantly different (100% vs. 90%, p = 0.25), while the specificity of HC2 was significantly lower than that of FISH (28% vs. 48%, p=0.003). FISH diagnosed fewer specimens as positive as compared to HC2 (62% vs. 79%).Conclusion: These preliminary data suggest that FISH testing may be useful for determining which patients with LSIL are most likely to have CIN 2+ on clinical follow-up.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 3","pages":"121-30"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29182065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of N(G)-nitro-L-arginine methyl ester and metallothioneins on excess nitric oxide toxicity in trinitrobenzene sulfonic acid-induced rat colitis. N(G)-硝基- l -精氨酸甲酯和金属硫蛋白对三硝基苯磺酸诱导大鼠结肠炎一氧化氮过量毒性的保护作用。

Analytical and quantitative cytology and histology Pub Date : 2010-06-01

Yilmaz Altuner, Adnan Ayhanci, Kismet Civi, Hilmi Ozden, Derya Ustuner, M Cengiz Ustuner, Hulyam Kurt

{"title":"Protective effects of N(G)-nitro-L-arginine methyl ester and metallothioneins on excess nitric oxide toxicity in trinitrobenzene sulfonic acid-induced rat colitis.","authors":"Yilmaz Altuner, Adnan Ayhanci, Kismet Civi, Hilmi Ozden, Derya Ustuner, M Cengiz Ustuner, Hulyam Kurt","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To evaluate the protective effects of N(G)-nitro-L-arginine methyl ester (L-NAME) and metallothioneins on excess nitric oxide toxicity in trinitrobenzene sulfonic acid (TNBS)-induced rat colitis.Study design: In this study, 70 rats were assigned to 7 groups of controls, and colitis was induced with 120 mg/kg TNBS, 35 mg/kg L-NAME, and 1 and 2 mg/kg metallothionein 1 (MT1) and metallothionein 2 (MT2), respectively. A day after the administration of TNBS, L-NAME, MT1 and MT2 were given intraperitoneally for 3 days to the experimental groups. After the administration of TNBS, dissections of the rats in the L-NAME, MT1 and MT2 groups were performed at 3-day periods under ether anesthesia, and whole blood, bone marrow and colon were obtained.Results: On the third day, red and white blood cell values were increased, while platelet and bone marrow granule cells decreased in the L-NAME- and TNBS-induced group. On the third day, all the blood values increased in MT1 (1 and 2 mg/kg) and MT2 (1 and 2 mg/kg) in the TNBS-administered groups. Histologic findings were macroscopic score, ulcer, loss of mucous cells, crypt abscess, inflammatory cyst, mucosa atrophy, edema, vascular dilatation and induced nitric oxide synthetase, which increased in the descending colon in the colitis rats, while it was decreased rats given L-NAME, MT1 and MT2 administration.Conclusion: The results suggest that MT1 and MT2 are more effective in protecting against the toxic effects of excess nitric oxide as compared with L-NAME in the colitis rats.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 3","pages":"166-73"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29183154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0