Analytical and quantitative cytology and histology最新文献_第9页

S100A8 cellular distribution in normal epithelium, hyperplasia, dysplasia and squamous cell carcinoma and its concentration in serum. S100A8在正常上皮、增生、不典型增生及鳞状细胞癌中的细胞分布及血清浓度。

Analytical and quantitative cytology and histology Pub Date : 2010-08-01

Oliver Driemel, Niko Escher, Günther Ernst, Christian Melle, Ferdinand von Eggeling

{"title":"S100A8 cellular distribution in normal epithelium, hyperplasia, dysplasia and squamous cell carcinoma and its concentration in serum.","authors":"Oliver Driemel, Niko Escher, Günther Ernst, Christian Melle, Ferdinand von Eggeling","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the function of S100A8, a member of the calcium-binding S100 protein family, in oral tumorigenesis. We analyzed its cellular distribution and its serum level in patients with squamous cell carcinoma and normal controls.Study design: We investigated the histopathologic features by tissue microarrays (TMAs) including 8 normal, 66 hyperplastic and dysplastic and 26 oral squamous cell carcinoma (OSCC) tissue cores. The serum level of S100A8 was measured by an enzyme-linked immunosorbent assay using 33 healthy volunteers, 20 patients with hyperproliferative lesions and 23 patients with OSCC.Results: The TMA analysis resulted in different findings. The strongest expression of S100A8 was found in severe dysplasias and carcinoma in situ. In tumor tissue an increased expression occurred only focally. In the normal tissue cores the epithelium showed a moderate reaction, but basal and parabasal cells were completely negative. The serum levels of S100A8 were marginally reduced in cancer patients. The expression between healthy controls and patients with hyperproliferative lesions displayed no difference.Conclusion: The expression of S100A8 is helpful only in the transition from severe dysplastic tissue to cancer.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 4","pages":"219-24"},"PeriodicalIF":0.0,"publicationDate":"2010-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29766140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Image cytometric validation of breast carcinoma markers (ER, HER2 and MIB-1) using tissue microarrays: rabbit monoclonal vs. FDA-approved antibodies. 使用组织微阵列对乳腺癌标志物(ER, HER2和MIB-1)的图像细胞术验证:兔单克隆与fda批准的抗体。

Analytical and quantitative cytology and histology Pub Date : 2010-08-01

Aziza Nassar, Christine M Norton, Diane Lawson, Cynthia Cohen

{"title":"Image cytometric validation of breast carcinoma markers (ER, HER2 and MIB-1) using tissue microarrays: rabbit monoclonal vs. FDA-approved antibodies.","authors":"Aziza Nassar, Christine M Norton, Diane Lawson, Cynthia Cohen","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To use the ACIS III (Dako, Carpinteria, California, U.S.A.) with tissue microarrays (TMAs) to compare rabbit monoclonal antibodies (RMab) for ER, HER2, and MIB-1 with FDA-approved monoclonal (FMab) and polyclonal (FPab) antibodies.Study design: TMAs of 43 breast cancers were used. Immunohistochemistry was performed using RMab (LabVision, Fremont, California, U.S.A.): ER (SP1; 1/100), HER2 (SP3; 1/100), and MIB-1 (SP6; 1/200). FMPab (Dako) used: ER (1D5; 1/50), HercepTest kit and MIB-1 (MIB-1; 1/160). The stained TMAs were quantitated visually and by image cytometry (ACIS III).Results: The overall agreement between RMab and FMab for ER using visual (98.45%) and image analysis (97.56%) was excellent, with a kappa level of 0.89 and 0.94, respectively. For HER2, the overall agreement between RMab and FPab was fair for visual (67.44%) and substantial (87.50%) for image analysis, with a kappa level of 0.32 and 0.72, respectively. For MIB-1, there was fair (64.29%) to poor (43.33%) agreement between MIB-1 RMab and FMab using visual and image analysis, with a kappa level of 0.47 and 0.16, respectively.Conclusion: RMabs for ER (SP1) and HER2 (SP3) are almost comparable to their counterpart, FDA antibodies; however, MIB-1 RMab (SP6) shows poor concordance with FMab in TMA. Image analysis shows a better concordance than visual quantitation assessment specifically for ER and HER2.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 4","pages":"192-200"},"PeriodicalIF":0.0,"publicationDate":"2010-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29766870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA damage in Mexican women with cervical dysplasia evaluated by comet assay. 用彗星测定法评估墨西哥宫颈发育不良妇女的DNA损伤。

Analytical and quantitative cytology and histology Pub Date : 2010-08-01

Elva I Cortés-Gutiérrez, Martha I Dávila-Rodríguez, Estela A Zamudio-González, Miguel E Aguado-Barrera, Javier Vargas-Villarreal, Ricardo M Cerda-Flores

引用次数: 0

Improved diagnostic accuracy of testicular biopsies for infertility through simplified criteria and a dedicated pathologist. 通过简化标准和专门的病理学家，提高了不孕症睾丸活检的诊断准确性。

Analytical and quantitative cytology and histology Pub Date : 2010-08-01

Hans Ivar Hanevik, Bjørn Logi Isfoss, Anette Bergh, Mette Friberg, Jarl A Kahn

{"title":"Improved diagnostic accuracy of testicular biopsies for infertility through simplified criteria and a dedicated pathologist.","authors":"Hans Ivar Hanevik, Bjørn Logi Isfoss, Anette Bergh, Mette Friberg, Jarl A Kahn","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To examine whether simplified histopathologic criteria and a dedicated pathologist could influence the diagnostic accuracy of testicular biopsy.Study design: Original reports from general pathologists on 99 consecutive testicular biopsies were retrospectively classified according to reported presence or absence of mature spermatids. A dedicated pathologist rediagnosed the material blindly according to the same criterion. The resulting data were compared with testicular sperm extraction (TESE) results from the in vitro fertilization laboratory.Results: General pathologists' diagnoses predicted TESE results with a positive predictive value (PPV) of 0.97 and a negative predictive value (NPV) of 0.78. The dedicated pathologist's diagnoses yielded a PPV of 1.0 and a significantly improved NPV of 0.96.Conclusion: Visualization of mature spermatids in a testicular biopsy is an excellent predictor of TESE results, especially in the hands of a dedicated pathologist. We therefore recommend simplified pathology reporting on testicular biopsies from azoospermic men and examination by a dedicated pathologist.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 4","pages":"214-8"},"PeriodicalIF":0.0,"publicationDate":"2010-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29766139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence in situ hybridization analysis and immunophenotyping of c-Kit/PDGFRA and Bcl-2 expression in gastrointestinal stromal tumors. 胃肠道间质瘤中c-Kit/PDGFRA和Bcl-2表达的荧光原位杂交分析和免疫表型分析。

Analytical and quantitative cytology and histology Pub Date : 2010-08-01

Marta Orsenigo, Silvia Brich, Carla Riva, Elena Conca, Rossella Bertulli, Palma Dileo, Alessandro Gronchi, Paolo G Casali, Marco A Pierotti, Elena Tamborini, Silvana Pilotti

{"title":"Fluorescence in situ hybridization analysis and immunophenotyping of c-Kit/PDGFRA and Bcl-2 expression in gastrointestinal stromal tumors.","authors":"Marta Orsenigo, Silvia Brich, Carla Riva, Elena Conca, Rossella Bertulli, Palma Dileo, Alessandro Gronchi, Paolo G Casali, Marco A Pierotti, Elena Tamborini, Silvana Pilotti","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate c-Kit/PDGFRA genomic alterations, KIT-PDGFRA coexpression in gastrointestinal stromal tumors (GISTs) and the role of Bcl-2.Study design: We analyzed 70 primary tumors, 6 recurrences, 4 metastases and 1 recurrence plus metastasis, all molecularly characterized. Alterations in gene copy number were detected by fluorescence in situ hybridization (FISH) and expression of KIT, PDGFRA and Bcl-2 by immunohistochemistry.Results: c-Kit/PDGFRA gene alterations affected 38% of all cases and 39% of primary tumors, with major changes accounting for 15% in both all the cases and primary tumors. Cytoplasmic KIT/PDGFRA coexpression was present in 96.5% of the c-Kit-mutated cases, 100% of the wt c-Kit/PDGFRA cases and 66.6% of the PDGFRA-mutated cases. Bcl-2 immunoreactivity was present in 70% of cases, with expression levels of +++ in 29%, ++ in 38% and + in 33%.Conclusion: FISH confirmed cytogenetic alterations in about 40% of primary GISTs at the onset. The high rate of c-Kit/PDGFRA coexpression suggests that both receptors are involved in oncogenicity and may affect imatinib efficacy. The assumption that Bcl-2 expression is supported by the KIT pathway and that its imatinib-mediated down-regulation contributes to autophagic cell death, although attractive, needs to be further confirmed.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 4","pages":"225-33"},"PeriodicalIF":0.0,"publicationDate":"2010-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29766141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical and experimental applications of botulinum toxins in humans: the prostate gland as example. 肉毒杆菌毒素在人体中的临床和实验应用:以前列腺为例。

Analytical and quantitative cytology and histology Pub Date : 2010-08-01

Andrea Benedetto Galosi

引用次数: 0

A pilot study on lipolytic effect of subcutaneous botulinum toxin injection in rabbits. 兔皮下注射肉毒毒素溶脂作用的初步研究。

Analytical and quantitative cytology and histology Pub Date : 2010-08-01

Mansooreh Bagheri, Behnam Moein Jahromi, Mandana Bagheri, Afshin Borhani Haghighi, Ali Noorafshan, Perikala Vijai Kumar, Gholamhossein Ranjbar Omrani

{"title":"A pilot study on lipolytic effect of subcutaneous botulinum toxin injection in rabbits.","authors":"Mansooreh Bagheri, Behnam Moein Jahromi, Mandana Bagheri, Afshin Borhani Haghighi, Ali Noorafshan, Perikala Vijai Kumar, Gholamhossein Ranjbar Omrani","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To determine whether botulinum toxin type A (BTX-A) exerts a lipolytic effect by interfering with acetylcholine transmission at the cholinergic parasympathetic nerve endings.Study design: Fifteen male rabbits were divided into 3 equal groups: 1 control group (A) and 2 case groups (B and C). The abdomens of all rabbits were divided into a 3 x 3-square grid. The groups received 9 subcutaneous injections of 0.9% normal saline, 1 U BTX-A (group B) and 2 U BTX-A (group C), respectively. Four weeks later the entire grid was excised from the abdominal area. Hematoxylin-eosin-stained tissue was used for stereologic analysis to estimate cell surface and volume in 100 randomly selected cells.Results: Gross thinning of subcutaneous fat and shattering and disappearance of fat globules were seen in both case groups. Fat cell volume was reduced by 65% in group B (p = 0.009) and 77% in group C (p = 0.009) compared to control animals. Fat cell surface also decreased by 51% in group B (p = 0.009) and 63% in group C rabbits (p = 0.009) compared to control animals.Conclusion: Our pilot animal study revealed a dose-dependent lipolytic effect of subcutaneous BTX-A injection.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 4","pages":"186-91"},"PeriodicalIF":0.0,"publicationDate":"2010-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29766869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunostaining for nucleophosmin in bone marrow trephine biopsy specimens in acute myeloid leukemias. 急性髓系白血病骨髓穿刺标本中核磷蛋白的免疫染色。

Analytical and quantitative cytology and histology Pub Date : 2010-08-01

Gaia Goteri, Antonio Zizzi, Simona Sabato, Antonello Costagliola, Daniela Stramazzotti, Corrado Rubini, Giancarlo Discepoli, Debora Capelli, Pietro Leoni

{"title":"Immunostaining for nucleophosmin in bone marrow trephine biopsy specimens in acute myeloid leukemias.","authors":"Gaia Goteri, Antonio Zizzi, Simona Sabato, Antonello Costagliola, Daniela Stramazzotti, Corrado Rubini, Giancarlo Discepoli, Debora Capelli, Pietro Leoni","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To evaluate the technicalfeasibility of nucleophosmin (NPM) staining and the problems of interpretations by pathologists in an academic regional hospital in Italy.Study design: Acute myeloid leukemia (AML) is a heterogeneous clonal disorder of hematopoietic progenitor cells that presents genetic abnormalities in several genes, including NPM. Mutations of the NPM gene occur in 35% of patients with AML with normal karyotype, causing cytoplasmic rather than nuclear localization of the protein. Because the NPM antibody recently became commercially available, we immunostained a series of diagnosed AML samples. We performed NPM immunostaining in 48 AML cases. NPM immunostaining was correlated with phenotypic and cytogenetic data.Results: Reactivity for NPM was exclusively nuclear in 31 cases (64.6%) and nuclear and cytoplasmic in 17 cases (35.4%). The distribution of NPM cytoplasmic staining was more frequently observed in cases with monocytic differentiation and with normal karyotype or with minor cytogenetic abnormalities (p < 0.05).Conclusion: NPM immunostaining is a feasible test, without problems of interpretation for pathologists, when the sections are optimally prepared and can be considered predictive of peculiar phenotypic and karyotype subtypes of AML, in addition to the well-known prognostic role.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 4","pages":"201-6"},"PeriodicalIF":0.0,"publicationDate":"2010-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29766871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphometric analysis in cytologic evaluation of papillary thyroid carcinoma. 形态计量学分析在甲状腺乳头状癌细胞学评价中的应用。

Analytical and quantitative cytology and histology Pub Date : 2010-08-01

Mehmet Kefeli, Ilkser Akpolat, Arzu Yildirim, Ahmet Tevfik Sunter, Bedri Kandemir

{"title":"Morphometric analysis in cytologic evaluation of papillary thyroid carcinoma.","authors":"Mehmet Kefeli, Ilkser Akpolat, Arzu Yildirim, Ahmet Tevfik Sunter, Bedri Kandemir","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To compare the morphometric features of papillary carcinomas with follicular neoplasias and benign lesions and to determine the potential role of nuclear morphometric features in their differential diagnosis.Study design: Morphometric features were investigated in the cytologic samples of 64 cases, including 27 benign lesions, 6 follicular neoplasias and 31 papillary carcinomas. We analyzed 6 morphometric parameters: nuclear area, nuclear perimeter, maximum diameter (MaxD), minimum diameter (MinD), form factor and ratio of MaxD/MinD.Results: There were no significant differences between the benign lesion and follicular neoplasia groups for any nuclear parameters. However, between benign lesions and papillary carcinomas, there were significant differences for all parameters, except for form factor. Between follicular neoplasms and papillary carcinomas, only the MaxD/MinD ratio was significantly different.Conclusion: The results of this study indicate that the ratio of MaxD/MinD is a distinct nuclear morphometric feature for distinguishing papillary carcinoma from other thyroid lesions, and it may be employed with other cytologic criteria in diagnosing problematic cases.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 4","pages":"234-8"},"PeriodicalIF":0.0,"publicationDate":"2010-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29766142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synovial sarcoma of the kidney: a report of 4 cases with pathologic appraisal and differential diagnostic review. 肾滑膜肉瘤:附4例病理鉴定及鉴别诊断复习报告。

Analytical and quantitative cytology and histology Pub Date : 2010-08-01

Yia Swam Tan, Lay Guat Ng, Sidney Kam-Hung Yip, Miah-Hiang Tay, Alvin Soon-Tiong Lim, Sim Leng Tien, Liang Cheng, Puay Hoon Tan

{"title":"Synovial sarcoma of the kidney: a report of 4 cases with pathologic appraisal and differential diagnostic review.","authors":"Yia Swam Tan, Lay Guat Ng, Sidney Kam-Hung Yip, Miah-Hiang Tay, Alvin Soon-Tiong Lim, Sim Leng Tien, Liang Cheng, Puay Hoon Tan","doi":"","DOIUrl":"","url":null,"abstract":"Background: Synovial sarcoma of the kidney is rare. It is clinicoradiologically indistinguishable from the more frequently encountered renal cell carcinoma. Histologically it needs to be differentiated from other spindle cell lesions occurring within the kidney, including a spectrum of benign to malignant tumors. Among malignant spindle cell tumors of the kidney, mimics of synovial sarcoma are sarcomatoid renal cell carcinoma, sarcomatoid urothelial carcinoma and other primary sarcomas, such as leiomyosarcoma and malignant fibrous histiocytoma.Cases: Four cases of synovial sarcoma originated in the kidney, with this report focusing on clinicopathologic and differential diagnostic features.Conclusion: The correct diagnosis of synovial sarcoma requires support by an immunohistochemical panel as well as adjunctive investigations like polymerase chain reaction and fluorescence in situ hybridization to determine the presence of the SYT-SSX fusion gene and translocation (X,18), respectively.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 4","pages":"239-45"},"PeriodicalIF":0.0,"publicationDate":"2010-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29766143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0