Elva I Cortés-Gutiérrez, Martha I Dávila-Rodríguez, Estela A Zamudio-González, Miguel E Aguado-Barrera, Javier Vargas-Villarreal, Ricardo M Cerda-Flores
{"title":"用彗星测定法评估墨西哥宫颈发育不良妇女的DNA损伤。","authors":"Elva I Cortés-Gutiérrez, Martha I Dávila-Rodríguez, Estela A Zamudio-González, Miguel E Aguado-Barrera, Javier Vargas-Villarreal, Ricardo M Cerda-Flores","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the association between the progressive stage of cervical dysplasia and DNA damage by comet assay.</p><p><strong>Study design: </strong>A hospital-based, unmatched, case-control study was performed. DNA damage levels (none, low, medium and high) in the cervical epithelial cells of 31 women (10 with low grade squamous intraepithelial lesions [LSIL], 10 with high grade [HSIL] and 11 with no cervical lesion) were evaluated using the comet assay.</p><p><strong>Results: </strong>A significant increase in medium DNA damage was observed in women with HSIL (17 +/- 8.9) relative to that in the control women (9 +/- 6.1). A significant increase in high DNA damage was also observed in women with LSIL (23 +/- 15.4) or HSIL (32 +/- 13.1) relative to that in the control women (12 +/- 7.9).</p><p><strong>Conclusion: </strong>These findings confirm that the grade of a cervical lesion correlates with the degree of genomic instability.</p>","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 4","pages":"207-13"},"PeriodicalIF":0.0000,"publicationDate":"2010-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"DNA damage in Mexican women with cervical dysplasia evaluated by comet assay.\",\"authors\":\"Elva I Cortés-Gutiérrez, Martha I Dávila-Rodríguez, Estela A Zamudio-González, Miguel E Aguado-Barrera, Javier Vargas-Villarreal, Ricardo M Cerda-Flores\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the association between the progressive stage of cervical dysplasia and DNA damage by comet assay.</p><p><strong>Study design: </strong>A hospital-based, unmatched, case-control study was performed. DNA damage levels (none, low, medium and high) in the cervical epithelial cells of 31 women (10 with low grade squamous intraepithelial lesions [LSIL], 10 with high grade [HSIL] and 11 with no cervical lesion) were evaluated using the comet assay.</p><p><strong>Results: </strong>A significant increase in medium DNA damage was observed in women with HSIL (17 +/- 8.9) relative to that in the control women (9 +/- 6.1). A significant increase in high DNA damage was also observed in women with LSIL (23 +/- 15.4) or HSIL (32 +/- 13.1) relative to that in the control women (12 +/- 7.9).</p><p><strong>Conclusion: </strong>These findings confirm that the grade of a cervical lesion correlates with the degree of genomic instability.</p>\",\"PeriodicalId\":76995,\"journal\":{\"name\":\"Analytical and quantitative cytology and histology\",\"volume\":\"32 4\",\"pages\":\"207-13\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical and quantitative cytology and histology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and quantitative cytology and histology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
DNA damage in Mexican women with cervical dysplasia evaluated by comet assay.
Objective: To investigate the association between the progressive stage of cervical dysplasia and DNA damage by comet assay.
Study design: A hospital-based, unmatched, case-control study was performed. DNA damage levels (none, low, medium and high) in the cervical epithelial cells of 31 women (10 with low grade squamous intraepithelial lesions [LSIL], 10 with high grade [HSIL] and 11 with no cervical lesion) were evaluated using the comet assay.
Results: A significant increase in medium DNA damage was observed in women with HSIL (17 +/- 8.9) relative to that in the control women (9 +/- 6.1). A significant increase in high DNA damage was also observed in women with LSIL (23 +/- 15.4) or HSIL (32 +/- 13.1) relative to that in the control women (12 +/- 7.9).
Conclusion: These findings confirm that the grade of a cervical lesion correlates with the degree of genomic instability.
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