Analytical and quantitative cytology and histology最新文献_第8页

Fractal dimension of cervical intraepithelial lesions on cytology smear. 宫颈上皮内病变细胞学涂片的分形维数。

Analytical and quantitative cytology and histology Pub Date : 2010-12-01

Pranab Dey, Nidhi Sharma, Swapan Samanta

{"title":"Fractal dimension of cervical intraepithelial lesions on cytology smear.","authors":"Pranab Dey, Nidhi Sharma, Swapan Samanta","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To explore the role of fractal dimension (FD) in distinguishing normal and different grades of cervical intraepithelial lesions.Study design: We selected 15 cases each of normal samples and low-grade intraepithelial lesions (LSIL) and 14 cases of high-grade intraepithelial lesions (HSIL). All HSIL cases were histopathology proven. The LSIL diagnosis was verified by two cytopathologists. In each case at least 10 abnormal cells were selected in LSIL and HSIL cases and 10 normal cells in normal smears. A total of 202, 157, and 184 cells were selected from the normal, LSIL, and HSIL groups, respectively. The FD was measured in nucleus of each cell by box counting FD method with the help of appropriate software.Results: The mean FD of the normal, LSIL, and HSIL nucleus was 1.2425 +/- 0.09188, 1.3504 +/- 0.6766, and 1.4379 +/- 0.08830, respectively. An analysis of variance test showed significant difference ofFD among the three groups (p < 0.0001).Conclusion: FD may be used as an additional factor to distinguish the dysplastic cells in the cervical cytology smear, which may be useful in automated screening in future.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 6","pages":"320-2"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29784398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computer system for cell counting in selected brain tumors at Ki-67 immunohistochemical staining. 脑肿瘤Ki-67免疫组化染色细胞计数计算机系统。

Analytical and quantitative cytology and histology Pub Date : 2010-12-01

Tomasz Markiewicz, Bartlomiej Grala, Wojciech Kozlowski, Stanislaw Osowski

{"title":"Computer system for cell counting in selected brain tumors at Ki-67 immunohistochemical staining.","authors":"Tomasz Markiewicz, Bartlomiej Grala, Wojciech Kozlowski, Stanislaw Osowski","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To present a computerized system for cell counting in histopathologic slides of meningioma and oligodendroglioma stained immunohistochemically against Ki-67 antigen and discuss the variability of tumor cell numbers in the field of view of analyzed neoplasms to standardize tumor cellularity.Study design: A computer program using an algorithm based on mathematical morphology was developed to perform quantitative evaluation of slides. That solution was combined with the Support Vector Machine used for classification of cell immunoreactivity.Results: The mean number of cells in the analyzed field of view from patients with meningioma was 623. Of these, 95% were in the 386-781 cells range. In oligodendrogliomas the mean was 474 cells and all results were in the 204-736 range. The mean relative discrepancy between results of our system and human expert score was 8%.Conclusion: The proposed system appeared to be an efficient tool for supporting histopathologic diagnosis. The applied sequential thresholding simulated well the human process of cell recognition. Cellularity of the analyzed tumors did not show stability within the specimens from different patients. The results were also highly variable in different fields of view obtained from the same patient.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 6","pages":"323-32"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29784399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantificational and statistical analysis of the differences in centrosomal features of untreated lung cancer cells and normal cells. 未经治疗的肺癌细胞与正常细胞中心体特征差异的定量统计分析。

Analytical and quantitative cytology and histology Pub Date : 2010-10-01

Dansheng Song, Inna Fedorenko, Marianna Pensky, Wei Qian, Melvyn S Tockman, Tatyana A Zhukov

{"title":"Quantificational and statistical analysis of the differences in centrosomal features of untreated lung cancer cells and normal cells.","authors":"Dansheng Song, Inna Fedorenko, Marianna Pensky, Wei Qian, Melvyn S Tockman, Tatyana A Zhukov","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To distinguish untreated lung cancer cells from normal cells through quantitative analysis and statistical inference of centrosomal features extracted from cell images.Study design: Recent research indicates that human cancer cell development is accompanied by centrosomal abnormalities. For quantitative analysis of centrosome abnormalities, high-resolution images of normal and untreated cancer lung cells were acquired. After the images were preprocessed and segmented, 11 features were extracted. Correlations among the features were calculated to remove redundant features. Ten nonredundant features were selected for further analysis. The mean values of 10 centrosome features were compared between cancer and normal cells by the two-sample t-test; distributions of the 10 features of cancer and normal centrosomes were compared by the two-sample Kolmogorov-Smirnov test.Results: Both tests reject the null hypothesis; the means and distributions of features coincide for normal and cancer cells. The 10 centrosome features separate normal from cancer cells at the 5% significance level and show strong evidence that all 10 features exhibit major differences between normal and cancer cells.Conclusion: Centrosomes from untreated cancer and normal bronchial epithelial cells can be distinguished through objective measurement and quantitative analysis, suggesting a new approach for lung cancer detection, early diagnosis and prognosis.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":" ","pages":"280-90"},"PeriodicalIF":0.0,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40119096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mean nuclear volume in complex atypical hyperplasia and adenocarcinoma of the endometrium. 子宫内膜复杂非典型增生和腺癌的平均核体积。

Analytical and quantitative cytology and histology Pub Date : 2010-10-01

Jasleen Kaur, Pranab Dey

引用次数: 0

Computer-aided classification of centroblast cells in follicular lymphoma. 滤泡性淋巴瘤中成中心细胞的计算机辅助分类。

Analytical and quantitative cytology and histology Pub Date : 2010-10-01

Kamel Belkacem-Boussaid, Michael Pennell, Gerard Lozanski, Arwa Shana'ah, Metin Gurcan

{"title":"Computer-aided classification of centroblast cells in follicular lymphoma.","authors":"Kamel Belkacem-Boussaid, Michael Pennell, Gerard Lozanski, Arwa Shana'ah, Metin Gurcan","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To distinguish centroblast cells from non-centroblast cells using a novel automated method in follicular lymphoma cases and measure its performance on cases obtained by a consensus of six pathologists.Study design: Geometric and color texture features were used in the training and testing of the supervised quadratic discriminant analysis classifier. The technique was trained and tested on a data set composed of 218 centroblast images and 218 non-centroblast images. Computer performance was tested by measuring sensitivity and specificity among cells classified as centroblast and non-centroblast by consensus of six board-certified hematopathologists.Results: Automated classification distinguished centroblast from non-centroblast cells with a classification accuracy of 82.56% and sensitivity and specificity of 86.67% and 86.96%, respectively, when the approach was tested.Conclusion: The novelty of our approach is the identification of the centroblast cells with prior information and the introduction of the principal component analysis in the spectral domain to extract texture color features.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"32 5","pages":"254-60"},"PeriodicalIF":0.0,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3078581/pdf/nihms252602.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29828945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coexistence of bladder prostatic-type polyp and urothelial papilloma. 膀胱前列腺型息肉与尿路上皮乳头状瘤共存。

Analytical and quantitative cytology and histology Pub Date : 2010-10-01

Teresa Pusiol, Maria Grazia Zorzi, Irene Piscioli

引用次数: 0

Usefulness and limitations of alpha-methylacyl-CoA racemase expression in the hepatobiliary system and pancreas: a wide tissue microarray analysis of normal and neoplastic epithelia. -甲基酰基辅酶a消消酶在肝胆系统和胰腺中的表达的有效性和局限性:正常和肿瘤上皮的广泛组织微阵列分析。

Analytical and quantitative cytology and histology Pub Date : 2010-10-01

Ana Mozos, Manel Sole, Rosa Miquel, Ana Petit, Alfons Nadal, Pedro Luis Fernández

{"title":"Usefulness and limitations of alpha-methylacyl-CoA racemase expression in the hepatobiliary system and pancreas: a wide tissue microarray analysis of normal and neoplastic epithelia.","authors":"Ana Mozos, Manel Sole, Rosa Miquel, Ana Petit, Alfons Nadal, Pedro Luis Fernández","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To establish the frequency, significance and clinical utility of alpha-methylacyl-CoA racemase (AMACR) expression in the hepatobiliary system by performing a wide immunohistochemical screening of AMACR expression.Study design: A total of 204 neoplastic, preneoplastic and normal samples from the hepatobiliary and pancreatic system were immunohistochemically studied on tissue microarrays for AMACR expression and correlated with clinicopathologic parameters.Results: Hepatocellular carcinomas (HCC) showed intense AMACR expression, which was significantly stronger than in nonmalignant tissues and correlated with good differentiation but not with evolution. Weak AMACR expression was observed in cholangiocarcinomas and preneoplastic pancreatic lesions. HCC showed stronger expression than cholangiocarcinomas and ductal pancreatic adenocarcinomas.Discussion: AMACR is strongly expressed in some neoplastic and preneoplastic lesions of the hepatobiIiary system, mainly HCC, and may play a role in malignant transformation and be useful in the differential diagnosis.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":" ","pages":"261-8"},"PeriodicalIF":0.0,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40119094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of image resolution and noise on estimating the fractal dimension of tissue specimens. 图像分辨率和噪声对组织标本分形维数估计的影响。

Analytical and quantitative cytology and histology Pub Date : 2010-10-01

Vanessa Dixon, Mauro Tambasco

{"title":"Effects of image resolution and noise on estimating the fractal dimension of tissue specimens.","authors":"Vanessa Dixon, Mauro Tambasco","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the effects of imaging system noise and resolution on the ability to estimate and distinguish relative differences in the fractal dimension of tissue specimens.Study design: Mathematically derived test images of known fractal dimension mimicking the complexity of epithelial morphology were created. The box-counting method was used to compute fractal dimension. To study the effects of resolution on fractal dimension, the test images were convolved with Gaussian point spread functions (PSF), and effects of noise were studied by adding Poisson and Gaussian noise. Application of these findings was illustrated by measuring the resolution and noise for a typical optical microscope and digital camera (OMDC) system.Results: Poor spatial resolution reduces the fractal dimension and has an increased adverse effect on higher dimensions. Fractal dimension can be estimated within 7% of the true dimension, and relative differences of 0.1 between dimensions are distinguishable provided the PSF of an imaging system has a full-width-at-half-maximum < or = 4 pixels and the contrast-to-noise ratio > 15. These conditions were satisfied by our OMDC.Conclusion: Effects of noise and resolution from a typical OMDC system do not significantly inhibit the ability to estimate and distinguish relative differences in the fractal dimension of tissue specimens.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":" ","pages":"269-79"},"PeriodicalIF":0.0,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40119095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mature cystic teratoma of the appendix: a case report. 阑尾成熟囊性畸胎瘤1例。

Analytical and quantitative cytology and histology Pub Date : 2010-10-01

Gwendolyn J Godfrey, Rania Bakkar, Hanan Farghaly

引用次数: 0

Prognostic significance of p53 and KAI-1 expression in patients with laryngeal squamous cell carcinoma. p53和KAI-1表达在喉鳞癌患者中的预后意义。

Analytical and quantitative cytology and histology Pub Date : 2010-10-01

Massimo Re, Giuseppe Magliulo, Eleonora Salvolini, Monia Orciani, Federico Maria Gioacchini, Gaia Goteri, Corrado Rubini

{"title":"Prognostic significance of p53 and KAI-1 expression in patients with laryngeal squamous cell carcinoma.","authors":"Massimo Re, Giuseppe Magliulo, Eleonora Salvolini, Monia Orciani, Federico Maria Gioacchini, Gaia Goteri, Corrado Rubini","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To examine the prognostic significance of the immunohistochemical expression of p53 and KAI-1 oncoproteins in patients with laryngeal squamous cell carcinoma.Study design: A retrospective evaluation was developed on 82 patients with primary laryngeal squamous cell carcinoma treated by primary surgery. For the immunohistochemical evaluation, formalin-fixed and paraffin-embedded tissue blocks from resection were used. Clinicopathologic data were correlated with the staining results.Results: The results of the immunohistochemical examination showed a statistically significant relationship between down-regulation of KAI-1 protein expression, increase of the histologic grading, and primary tumors associated with lymph node metastases. On the other hand, no statistical correlation was evidenced between p53 up-regulation and clinicopathologic data or between the two markers.Conclusion: Our results suggest that KAI-1 may become an important biomarker that could help in selection of high-risk patients with laryngeal squamous cell carcinoma who may benefit from more aggressive therapy or chemoprevention.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":" ","pages":"247-53"},"PeriodicalIF":0.0,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40119093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0