Analytical and quantitative cytology and histology最新文献_第2页

Differential diagnosis of follicular tumor by expert systems based on a set of quantitative features of thyrocyte nuclei and aggregates. 基于甲状腺细胞核和聚集体定量特征的专家系统对滤泡性肿瘤的鉴别诊断。

Analytical and quantitative cytology and histology Pub Date : 2012-04-01

Vladimir Kirillov, Olga Emeliyanova

{"title":"Differential diagnosis of follicular tumor by expert systems based on a set of quantitative features of thyrocyte nuclei and aggregates.","authors":"Vladimir Kirillov, Olga Emeliyanova","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To develop expert systems for classification of follicular thyroid tumor at a preoperative stage.Study design: Fine needle aspiration biopsy of the thyroid gland with a histologic conclusion of follicular cancer and follicular adenoma were the object of the morphometric study. General sample size was 4500 nuclei and 3000 aggregates.Results: Quantitative regularities of pathologic changes in thyrocyte nuclei and aggregates in follicular cancer and follicular adenoma were revealed. Threshold values and weighting coefficients of quantitative features of thyrocyte nuclei and aggregates characterizing cancer made the basis of the two expert systems. Expert systems included standard 2-D S-matrix containing threshold values of nuclei and aggregates in cancer and their weighting coefficients as well as 1-D scientific X-matrix designed for filling with quantitative features of the studied object. The diagnosis was verified by the value of a diagnostic index by means of comparing feature values in the corresponding elements of S- and X-matrices. After that, a diagnostic index was calculated taking into account the features' weighting coefficient.Conclusion: The developed expert systems based on a set of quantitative features of thyrocyte nuclei and aggregates will allow assessing the malignant potential of a follicular thyroid tumor at a preoperative stage.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"34 2","pages":"86-95"},"PeriodicalIF":0.0,"publicationDate":"2012-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30632998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prostate cancer gene 3 urine assay cutoff in diagnosis of prostate cancer: a validation study on an Italian patient population undergoing first and repeat biopsy. 前列腺癌基因3尿检测在前列腺癌诊断中的切断:一项对意大利患者进行首次和重复活检的验证研究。

Analytical and quantitative cytology and histology Pub Date : 2012-04-01

Enrico Bollito, Stefano De Luca, Matteo Cicilano, Roberto Passera, Susanna Grande, Carmen Maccagnano, Susanna Cappia, Angela Milillo, Francesco Montorsi, Roberto Mario Scarpa, Mauro Papotti, Donato Franco Randone

{"title":"Prostate cancer gene 3 urine assay cutoff in diagnosis of prostate cancer: a validation study on an Italian patient population undergoing first and repeat biopsy.","authors":"Enrico Bollito, Stefano De Luca, Matteo Cicilano, Roberto Passera, Susanna Grande, Carmen Maccagnano, Susanna Cappia, Angela Milillo, Francesco Montorsi, Roberto Mario Scarpa, Mauro Papotti, Donato Franco Randone","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To determine an optimal prostate cancer gene 3 (PCA3) cutoff in predicting prostate cancer in Italian patients undergoing first or repeat biopsy.Study design: In this observational multicenter study 1246 men with elevated prostate specific antigen (PSA) and negative digital rectal examination, with prostate biopsy after PCA3 assessment, were divided into two groups submitted to PCA3 testing before or after previous negative biopsies. Ideal PCA3 cutoff was identified using area under the curve of the receiver operating characteristic analysis. Various cutoff values were used to determine the best predictive score. Univariate and multivariate logistic regression models compared age, PSA, free-PSA, and PCA3 score to predict prostate cancer.Results: PCA3 cutoff 39-50 had the highest accuracy in the repeat biopsy group in which cutoff of 39 could have avoided 51.9% negative repeat biopsies, eventually missing 7.8% of cancers (all low risk); cutoff of 50 would have prevented 56.5% of negative repeat biopsies, missing 29 tumors (10.3%), 5 potentially aggressive. The PCA3 test performed poorly in the first biopsy group.Conclusion: We confirm the usefulness of PCA3 in Italian men with a previous negative biopsy. We achieved the best performance at a cutoff of 39. PCA3 did not perform better than PSA in non-biopsy-selected men.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"34 2","pages":"96-104"},"PeriodicalIF":0.0,"publicationDate":"2012-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30632999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative image analysis of gamma-H2AX foci induced by ionizing radiation applying open source programs. 应用开源程序定量分析电离辐射诱导的γ - h2ax聚焦。

Analytical and quantitative cytology and histology Pub Date : 2012-04-01

Jorge Ernesto González, Manual Lee, Joan Francesc Barquinero, Marco Valente, Sandrine Roch-Lefèvre, Omar García

{"title":"Quantitative image analysis of gamma-H2AX foci induced by ionizing radiation applying open source programs.","authors":"Jorge Ernesto González, Manual Lee, Joan Francesc Barquinero, Marco Valente, Sandrine Roch-Lefèvre, Omar García","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To test a CellProfiler pipeline for automated counting and characterization of gamma-H2AX foci in color images of human cultured cells.Study design: A431 cells were irradiated and stained for gamma-H2AX foci detection. Sets of color images were analyzed visually, and findings were compared with those using FociCounter and CellProfiler software.Results: The CellProfiler pipeline includes some proprieties not present in FociCounter, such as the automatic detection of nuclei, the detection of touching nuclei and the rejection of nuclei that touch the border of the image. The time required for manual operation is associated with the number of images analyzed visually or by FociCounter but not for the CellProfiler program. CellProfiler reduced manual operation time and is about 4 times faster than semiautomatic detection using FociCounter and 10 times faster than visual counting.Conclusion: We conclude that CellProfiler and FociCounter are reliable tools for measuring gamma-H2AX foci. However, CellProfiler overcomes the limitations of the FociCounter program and is able to detect nuclei automatically, saving considerable manual operation.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"34 2","pages":"66-71"},"PeriodicalIF":0.0,"publicationDate":"2012-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30632995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifractal spectrum differentiation of well-differentiated adenocarcinoma from complex atypical hyperplasia of the uterus. 子宫复杂非典型增生与高分化腺癌的多重分形谱鉴别。

Analytical and quantitative cytology and histology Pub Date : 2012-04-01

Adarsh Barwad, Pranab Dey

{"title":"Multifractal spectrum differentiation of well-differentiated adenocarcinoma from complex atypical hyperplasia of the uterus.","authors":"Adarsh Barwad, Pranab Dey","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To introduce a new field of multifractal spectrum in distinguishing between endometrial well-differentiated adenocarcinoma (WDAC) and complex atypical hyperplasia (CAH).Study design: Thirteen cases of CAH and 16 of WDAC were selected from radical hysterectomy specimens, and multifractal spectrum was measured from at least 4-5 representative digitized images of each case. The data were collected from f(alpha) vs. alpha curves. The values of alpha max, alpha min, and their difference delta alpha (alpha max-alpha min) were recorded and the data compared.Results: The mean +/- SD of alpha max, alpha min, and delta alpha of CAH were 2.36357 +/- 0.111623, 1.71357 +/- 0.032160, and 0.64214 +/- 0.094248, respectively. The mean +/- SD of alpha max, alpha min, and delta of WDAC were 2.50640 +/- 0.104545, 1.72100 +/- 0.036436, and 0.77620 +/- 0.108268, respectively. The mean of alpha max, alpha min, and delta alpha of WDAC were higher than in CAH. Mann-Whitney U test showed significant difference (p < 0.0001) of alpha max and delta alpja of WDAC and CAH.Conclusion: Multifractal dimension is significantly different in WDAC and CAH. The multifractal dimension is a new area in pathology. This study demonstrates the potential usefulness of multifractal analysis in histopathology.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"34 2","pages":"105-8"},"PeriodicalIF":0.0,"publicationDate":"2012-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30633000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative study of oral mucosa micronuclei in smokers and alcoholic smokers. 吸烟者与酗酒者口腔黏膜微核的比较研究。

Analytical and quantitative cytology and histology Pub Date : 2012-02-01

Lidiana Ubiña Oliveira, Celina Faig Lima, Miguel Angelo Castillo Salgado, Ivan Balducci, Janete Dias Almeida

{"title":"Comparative study of oral mucosa micronuclei in smokers and alcoholic smokers.","authors":"Lidiana Ubiña Oliveira, Celina Faig Lima, Miguel Angelo Castillo Salgado, Ivan Balducci, Janete Dias Almeida","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To compare the frequency of micronuclei and metanucleated anomalies in the oral mucosa of smokers, alcoholic smokers, and nonsmokers.Study design: Three groups were studied: group I, 15 smokers; group II, 16 alcoholic smokers; and group III, 20 nonsmokers. Three smears from the lateral left border of the tongue were processed for Feulgen staining. A minimum of 300 cells per participant were examined for the quantification of micronuclei and metanucleated anomalies.Results: The Kruskal-Wallis test showed no significant difference in the frequency of micronuclei (p = 0.602) or karyorrhexis (p = 0.114) among the three groups, but there was a significant difference in the frequency of broken eggs, binucleated cells, and karyolysis (p = 0.001). Spearman's correlation indicated an influence of the number of cigarettes per day on micronuclei frequency. Tobacco caused significant alterations in the exfoliative cytology (broken eggs, binucleated cells, and karyolysis) of chronic smokers, but not in the frequency of micronuclei or karyorrhexis, despite the observation of a larger absolute number of micronuclei in group II.Conclusion: The action of genotoxic agents (tobacco and alcohol) causes alterations in the frequency of micronuclei and metanucleated anomalies.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"34 1","pages":"9-14"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30621926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Karyometry of nuclear phenotypes in cutaneous squamous cell cancer. 皮肤鳞状细胞癌细胞核表型的核计量学研究。

Analytical and quantitative cytology and histology Pub Date : 2012-02-01

Peter H Bartels, Hubert G Bartels, David S Alberts, Michael Yozwiak, Anil R Prasad, Evan S Glazer, Robert S Krouse

{"title":"Karyometry of nuclear phenotypes in cutaneous squamous cell cancer.","authors":"Peter H Bartels, Hubert G Bartels, David S Alberts, Michael Yozwiak, Anil R Prasad, Evan S Glazer, Robert S Krouse","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To establish the karyometric characteristics of the two main nuclear phenotypes in cutaneous squamous cell cancer (cSCC) lesions.Study design: The clinical materials comprised 75 cases of cSCC, 38 with aggressive lesions and 37 with nonaggressive lesions. High-resolution images of 100 nuclei per case were recorded. Data were partitioned into four subgroups covering the range of lesion progression. Four discriminant functions were derived to distinguish aggressive from nonaggressive lesions. The most typical nuclei from the phenotype predominant in aggressive lesions and nonaggressive lesions were separated out by thresholding on the discriminant function score axes. For these homogeneous sets of nuclei the karyometric features were computed.Results: The nuclear populations in cSCC lesions are a very heterogeneous set. There are two axes of dispersion, along the line of lesion progression and between aggressive and nonaggressive lesions. The analysis faces the difficulty that lesions from both diagnostic categories contain nuclei of the same two phenotypes with the difference between categories consisting only of differences in proportion of the two phenotypes.Conclusion: The nuclei of the aggressive phenotype I and nonaggressive phenotype II have substantially different chromatin patterns and can be distinguished with > 90% correct recognition rate.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"34 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074412/pdf/nihms-586533.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30621925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of performance characteristics of five cell cycle-related immunohistochemical markers and human papillomavirus genotyping in the diagnosis of cervical squamous cell carcinoma precursor lesions. 五种细胞周期相关免疫组织化学标志物及人乳头瘤病毒基因分型在宫颈鳞状细胞癌前体病变诊断中的表现特点分析

Analytical and quantitative cytology and histology Pub Date : 2012-02-01

Pablo Conesa-Zamora, Javier Trujillo-Santos, Francisco Javier Orantes-Casado, Sebastián Ortiz-Reina, Daniel Torres-Moreno, Miguel Pérez-Guillermo

{"title":"Analysis of performance characteristics of five cell cycle-related immunohistochemical markers and human papillomavirus genotyping in the diagnosis of cervical squamous cell carcinoma precursor lesions.","authors":"Pablo Conesa-Zamora, Javier Trujillo-Santos, Francisco Javier Orantes-Casado, Sebastián Ortiz-Reina, Daniel Torres-Moreno, Miguel Pérez-Guillermo","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the performance values of a set of five immunohistochemical markers involved in cell cycle regulation as a potential aid in the differential diagnosis between squamous intraepithelial lesions (SILs) and normal or benign conditions of the uterine cervix.Study design: Results from immunohistochemical evaluation of p16, cyclin D1, p53, Ki67, and ProEx C markers and human papillomavirus genotyping were collected from a previous study performed on 37 normal or benign cervices, 39 low grade SILs and 73 high grade SILs. A multivariate analysis was used to examine the specific diagnostic value of each marker and to ascertain those most relevant for SIL diagnosis. For markers with good data fit, sensitivity, specificity, accuracy, area under the receiver operating characteristic curve, and integrated discrimination improvement, were calculated.Results: Among individual markers, ProEx C showed the best specificity; p16 displayed the highest sensitivity and area under receiver operating characteristic curve for SIL diagnosis. Integrated discrimination improvement demonstrated that the p16 plus ProEx C model has better discrimination capacity than p16 plus Ki67 or ProEx C plus Ki67.Conclusion: Use of ProEx C alone or in combination with p16 could provide useful information for SIL diagnosis.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"34 1","pages":"49-55"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30622935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormone receptors expression in phyllodes tumors of the breast. 乳腺叶状肿瘤中激素受体的表达。

Analytical and quantitative cytology and histology Pub Date : 2012-02-01

Yeong-Hui Kim, Ga-Eon Kim, Ji Shin Lee, Jae Hyuk Lee, Jong Hee Nam, Chan Choi, Min Ho Park, Jung Han Yoon

{"title":"Hormone receptors expression in phyllodes tumors of the breast.","authors":"Yeong-Hui Kim, Ga-Eon Kim, Ji Shin Lee, Jae Hyuk Lee, Jong Hee Nam, Chan Choi, Min Ho Park, Jung Han Yoon","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To ascertain the hormonal receptor profiles of the epithelial and stromal components of phyllodes tumors (PTs) and determine their relationship with stromal proliferation.Study design: Eighty-two PTs (50 benign, 22 borderline, and 10 malignant) were studied. Automated immunohistochemical staining for estrogen receptor (ER)-alpha and -beta, progesterone receptor (PR), androgen receptor (AR), and Ki-67 was performed using tissue microarray blocks, and their expression was assessed in both the stromal and epithelial components.Results: The epithelial component demonstrated the expression for ER-alpha (45.6%, 36 of 79), ER-beta (37.2%, 29 of 78), PR (91.1%, 72 of 79), and AR (10.1%, 8 of 79). The stromal component was positive for ER-beta (29.3%, 24 of 82) only. The epithelial expression of ER-beta was found to be significantly correlated with the epithelial expression of AR (r = 0.352, p = 0.002). No association was found between hormone receptor expression and PT tumor grade. Stromal Ki-67 expression was statistically correlated with epithelial ER-beta, epithelial AR, and stromal ER-beta expression.Conclusion: Epithelial and stromal ER-beta and epithelial AR expression in PTs was correlated with the proliferative rate in the stromal component. Immunohistochemical examination of ER-beta and AR may have some impact on the postoperative management of patients with PTs.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"34 1","pages":"41-8"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30621930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micronucleus scoring in urine cytology specimen by DNA-specific stain. dna特异性染色法在尿细胞学标本中的微核评分。

Analytical and quantitative cytology and histology Pub Date : 2012-02-01

Shelly Sharma, Pranab Dey, Uma Nahar Saikia, Raje Nijhawan

{"title":"Micronucleus scoring in urine cytology specimen by DNA-specific stain.","authors":"Shelly Sharma, Pranab Dey, Uma Nahar Saikia, Raje Nijhawan","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To evaluate the role of micronuclei (MN) scoring in urinary cytology of bladder transitional cell carcinoma (TCC).Study design: Eighty cases of freshly voided urine samples were used for scoring of MN using acridine orange (AO)-stained smears under fluorescence microscope and routine Papanicolaou-stained smears under light microscopy. MN score for cytologic lesions was compared.Results: In AO-stained slides, the mean MN scores were 0.171 +/- 0.395 and 1.489 +/- 1.871 in smears with \"no malignant cells seen\" and TCC cases, respectively. In Papanicolaou-stained slides the mean MN scores were 0.194 +/- 0.419 and 1.84 +/- 1.876 in smears with \"no malignant cells seen,\" and TCC cases, respectively. Independent sample t test showed significant difference of MN scoring in Papanicolaou-stain and AO stain in no malignancy vs. TCC. Pearson's correlation test showed strong positive correlation between mean MN score by AO and Papanicolaou stains and Pearson's correlation coefficient.Conclusion: MN score was significantly high in TCC cases, which may be due to chromosomal damage in bladder carcinoma. In difficult cases, MN may help distinguish benign from malignant cells in urine samples. Routine Papanicolaou stain may also be used for MN score because DNA-specific AO-stained smear gives almost similar results.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"34 1","pages":"56-60"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30622936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Angiogenetic pathway as a therapeutic target in renal cell carcinoma. 血管生成途径作为肾细胞癌的治疗靶点。

Analytical and quantitative cytology and histology Pub Date : 2012-02-01

Maristella Bianconi, Mario Scartozzi, Luca Faloppi, Alessandro Bittoni, Elena Maccaroni, Riccardo Giampieri, Michela Del Prete, Luciano Burattini, Stefano Cascinu

引用次数: 0