Clinical performance of human papillomavirus E6, E7 mRNA flow cytometric assay compared to human papillomavirus DNA typing.

Analytical and quantitative cytology and histology Pub Date : 2011-12-01

Christine Kottaridi, Sotirios Tsiodras, Aris Spathis, Aikaterini Chranioti, Asimakis Pappas, Dimitrios Kassanos, Ioannis Panayiotides, Petros Karakitsos

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Abstract

Objective: To use flow cytometry to screen cervical samples for the overexpression of human papillomavirus (HPV) E6 and E7 mRNA and compare the performance of this assay with an HPV DNA array for the detection of high-grade cervical lesions.

Study design: Cervical samples were analyzed for HPV DNA by clinical arrays, and the overexpression of E6 and E7 viral oncogenes was monitored using an HPV mRNA detection kit that quantifies the intracellular HPV E6 and E7 mRNA on a cell-by-cell basis.

Results: HPV positivity increased with severity of histologic lesions. On the basis of histology-confirmed CIN 2+ cases the specificity of HPV assay was 73.9% (95% CI 66.07, 80.88), whereas it was 39.3% (95% CI 31.85, 47.1) for the DNA assay.

Conclusion: The HPV assay provides an early predictor of persistent HPV infection and may improve cervical cancer screening by increasing the specificity of detecting high-grade lesions.

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临床表现人乳头瘤病毒E6、E7 mRNA流式细胞测定比较人乳头瘤病毒DNA分型。

目的:利用流式细胞术筛查宫颈标本中人乳头瘤病毒(HPV) E6和E7 mRNA的过表达，并与HPV DNA阵列检测宫颈高级别病变的性能进行比较。研究设计:通过临床阵列分析宫颈样本的HPV DNA，并使用HPV mRNA检测试剂盒监测E6和E7病毒癌基因的过表达，该试剂盒以细胞为单位定量细胞内HPV E6和E7 mRNA。结果:HPV阳性随组织学病变严重程度增高。在组织学证实的CIN 2+病例中，HPV检测的特异性为73.9% (95% CI 66.07, 80.88)，而DNA检测的特异性为39.3% (95% CI 31.85, 47.1)。结论:HPV检测提供了持续HPV感染的早期预测因子，并可能通过增加检测高级别病变的特异性来改善宫颈癌筛查。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Analytical and quantitative cytology and histology

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