Farveen Marican Abu Backer, Nik Raihan Nik Mustapha, Nor Hayati Othman
{"title":"联合表达p161NK4a和p27Kip1，但不表达p21WAF1，可区分宫颈内膜癌和子宫内膜腺癌。","authors":"Farveen Marican Abu Backer, Nik Raihan Nik Mustapha, Nor Hayati Othman","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To differentiate endocervical adenocarcinoma (ECA) from endometrial adenocarcinoma (EMA) using p16INK4a, p21WAF1 and p27Kip1.</p><p><strong>Study design: </strong>The distinction between ECA and EMA can be problematic on small biopsies or in advanced tumor stage. Immunohistochemistry was done on paraffin-embedded tissue sections of hysterectomy specimens of all types of ECA and EMA. Protein expression was scored as 0 to 3 depending on percentage of positive tumor cells.</p><p><strong>Results: </strong>A total of 40 ECA and 92 EMA cases were examined. For ECA, 32 of 40 (80.0%) cases had score of 3 with p16INK4a expressions; 23 of 92 (25.0%) cases of EMA had similar scores. For p21WAF1 expressions, 28 of 40 (70.0%) ECA cases scored 3 and 72 of 82 (78.2%) of EMA had a similar score. p27Kip1 scored 3 in 17 of 40 (42.5%) ECA cases; only 14 of 92 (15.2%) cases of EMA cases had such a score. We observed significant higher expressions of p16INK4a (p < 0.001) and p27Kip1 (p = 0.001) in ECA than in EMA. Combined positive expression and combined negative expression of p16INK4a and p27Kip1 strongly differentiates ECA from EMA (< 0.0001).</p><p><strong>Conclusion: </strong>ECA could be strongly differentiated from EMA based on the expressions of combined p16INK4a and p27Kip1.</p>","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 5","pages":"283-8"},"PeriodicalIF":0.0000,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Combined expression of p161NK4a and p27Kip1, but not p21WAF1, differentiates endocervical from endometrial adenocarcinoma.\",\"authors\":\"Farveen Marican Abu Backer, Nik Raihan Nik Mustapha, Nor Hayati Othman\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To differentiate endocervical adenocarcinoma (ECA) from endometrial adenocarcinoma (EMA) using p16INK4a, p21WAF1 and p27Kip1.</p><p><strong>Study design: </strong>The distinction between ECA and EMA can be problematic on small biopsies or in advanced tumor stage. Immunohistochemistry was done on paraffin-embedded tissue sections of hysterectomy specimens of all types of ECA and EMA. Protein expression was scored as 0 to 3 depending on percentage of positive tumor cells.</p><p><strong>Results: </strong>A total of 40 ECA and 92 EMA cases were examined. For ECA, 32 of 40 (80.0%) cases had score of 3 with p16INK4a expressions; 23 of 92 (25.0%) cases of EMA had similar scores. For p21WAF1 expressions, 28 of 40 (70.0%) ECA cases scored 3 and 72 of 82 (78.2%) of EMA had a similar score. p27Kip1 scored 3 in 17 of 40 (42.5%) ECA cases; only 14 of 92 (15.2%) cases of EMA cases had such a score. We observed significant higher expressions of p16INK4a (p < 0.001) and p27Kip1 (p = 0.001) in ECA than in EMA. Combined positive expression and combined negative expression of p16INK4a and p27Kip1 strongly differentiates ECA from EMA (< 0.0001).</p><p><strong>Conclusion: </strong>ECA could be strongly differentiated from EMA based on the expressions of combined p16INK4a and p27Kip1.</p>\",\"PeriodicalId\":76995,\"journal\":{\"name\":\"Analytical and quantitative cytology and histology\",\"volume\":\"33 5\",\"pages\":\"283-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical and quantitative cytology and histology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and quantitative cytology and histology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Combined expression of p161NK4a and p27Kip1, but not p21WAF1, differentiates endocervical from endometrial adenocarcinoma.
Objective: To differentiate endocervical adenocarcinoma (ECA) from endometrial adenocarcinoma (EMA) using p16INK4a, p21WAF1 and p27Kip1.
Study design: The distinction between ECA and EMA can be problematic on small biopsies or in advanced tumor stage. Immunohistochemistry was done on paraffin-embedded tissue sections of hysterectomy specimens of all types of ECA and EMA. Protein expression was scored as 0 to 3 depending on percentage of positive tumor cells.
Results: A total of 40 ECA and 92 EMA cases were examined. For ECA, 32 of 40 (80.0%) cases had score of 3 with p16INK4a expressions; 23 of 92 (25.0%) cases of EMA had similar scores. For p21WAF1 expressions, 28 of 40 (70.0%) ECA cases scored 3 and 72 of 82 (78.2%) of EMA had a similar score. p27Kip1 scored 3 in 17 of 40 (42.5%) ECA cases; only 14 of 92 (15.2%) cases of EMA cases had such a score. We observed significant higher expressions of p16INK4a (p < 0.001) and p27Kip1 (p = 0.001) in ECA than in EMA. Combined positive expression and combined negative expression of p16INK4a and p27Kip1 strongly differentiates ECA from EMA (< 0.0001).
Conclusion: ECA could be strongly differentiated from EMA based on the expressions of combined p16INK4a and p27Kip1.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
