Combined expression of p161NK4a and p27Kip1, but not p21WAF1, differentiates endocervical from endometrial adenocarcinoma.

Analytical and quantitative cytology and histology Pub Date : 2011-10-01

Farveen Marican Abu Backer, Nik Raihan Nik Mustapha, Nor Hayati Othman

{"title":"Combined expression of p161NK4a and p27Kip1, but not p21WAF1, differentiates endocervical from endometrial adenocarcinoma.","authors":"Farveen Marican Abu Backer, Nik Raihan Nik Mustapha, Nor Hayati Othman","doi":"","DOIUrl":null,"url":null,"abstract":"Objective: To differentiate endocervical adenocarcinoma (ECA) from endometrial adenocarcinoma (EMA) using p16INK4a, p21WAF1 and p27Kip1.Study design: The distinction between ECA and EMA can be problematic on small biopsies or in advanced tumor stage. Immunohistochemistry was done on paraffin-embedded tissue sections of hysterectomy specimens of all types of ECA and EMA. Protein expression was scored as 0 to 3 depending on percentage of positive tumor cells.Results: A total of 40 ECA and 92 EMA cases were examined. For ECA, 32 of 40 (80.0%) cases had score of 3 with p16INK4a expressions; 23 of 92 (25.0%) cases of EMA had similar scores. For p21WAF1 expressions, 28 of 40 (70.0%) ECA cases scored 3 and 72 of 82 (78.2%) of EMA had a similar score. p27Kip1 scored 3 in 17 of 40 (42.5%) ECA cases; only 14 of 92 (15.2%) cases of EMA cases had such a score. We observed significant higher expressions of p16INK4a (p < 0.001) and p27Kip1 (p = 0.001) in ECA than in EMA. Combined positive expression and combined negative expression of p16INK4a and p27Kip1 strongly differentiates ECA from EMA (< 0.0001).Conclusion: ECA could be strongly differentiated from EMA based on the expressions of combined p16INK4a and p27Kip1.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 5","pages":"283-8"},"PeriodicalIF":0.0000,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and quantitative cytology and histology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To differentiate endocervical adenocarcinoma (ECA) from endometrial adenocarcinoma (EMA) using p16INK4a, p21WAF1 and p27Kip1.

Study design: The distinction between ECA and EMA can be problematic on small biopsies or in advanced tumor stage. Immunohistochemistry was done on paraffin-embedded tissue sections of hysterectomy specimens of all types of ECA and EMA. Protein expression was scored as 0 to 3 depending on percentage of positive tumor cells.

Results: A total of 40 ECA and 92 EMA cases were examined. For ECA, 32 of 40 (80.0%) cases had score of 3 with p16INK4a expressions; 23 of 92 (25.0%) cases of EMA had similar scores. For p21WAF1 expressions, 28 of 40 (70.0%) ECA cases scored 3 and 72 of 82 (78.2%) of EMA had a similar score. p27Kip1 scored 3 in 17 of 40 (42.5%) ECA cases; only 14 of 92 (15.2%) cases of EMA cases had such a score. We observed significant higher expressions of p16INK4a (p < 0.001) and p27Kip1 (p = 0.001) in ECA than in EMA. Combined positive expression and combined negative expression of p16INK4a and p27Kip1 strongly differentiates ECA from EMA (< 0.0001).

Conclusion: ECA could be strongly differentiated from EMA based on the expressions of combined p16INK4a and p27Kip1.

本刊更多论文

联合表达p161NK4a和p27Kip1，但不表达p21WAF1，可区分宫颈内膜癌和子宫内膜腺癌。

目的:利用p16INK4a、p21WAF1和p27Kip1鉴别宫颈内膜腺癌(ECA)和子宫内膜腺癌(EMA)。研究设计:在小活检或肿瘤晚期，ECA和EMA的区别可能会有问题。对各类型ECA和EMA子宫切除术标本石蜡包埋组织切片进行免疫组化处理。根据阳性肿瘤细胞的百分比将蛋白表达评分为0到3。结果:共检查ECA 40例，EMA 92例。对于ECA, 40例患者中32例(80.0%)评分为3分，p16INK4a表达;92例EMA中有23例(25.0%)的评分相似。对于p21WAF1表达，40例ECA患者中有28例(70.0%)得分为3分，82例EMA患者中有72例(78.2%)得分相似。40例ECA患者中有17例(42.5%)p27Kip1评分为3;92例EMA中仅有14例(15.2%)有此评分。我们观察到p16INK4a (p < 0.001)和p27Kip1在ECA中的表达明显高于EMA。p16INK4a和p27Kip1的联合阳性表达和联合阴性表达将ECA与EMA区分开来(< 0.0001)。结论:基于p16INK4a和p27Kip1的联合表达，ECA可与EMA强分化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Analytical and quantitative cytology and histology

自引率

0.00%

发文量