Microscopy (Oxford, England)最新文献_第4页

Wire-tracking of bent electric cable using X-ray CT and deep active learning. 利用 X 射线 CT 和深度主动学习对弯曲电缆进行电线跟踪。

Microscopy (Oxford, England) Pub Date : 2024-12-05 DOI: 10.1093/jmicro/dfae028

Yutaka Hoshina, Takuma Yamamoto, Shigeaki Uemura

引用次数: 0

Correction to: Microstructural observation of casein micelles in milk by cryo-electron microscopy of vitreous sections (CEMOVIS). 更正：通过玻璃体切片冷冻电镜（CEMOVIS）观察牛奶中酪蛋白胶束的微观结构。

Microscopy (Oxford, England) Pub Date : 2024-11-09 DOI: 10.1093/jmicro/dfae053

引用次数: 0

Dose-efficient phase-contrast imaging of thick weak phase objects via OBF STEM using a pixelated detector. 利用像素化探测器，通过 OBF STEM 对厚的弱相位物体进行具有剂量效率的相位对比成像。

Microscopy (Oxford, England) Pub Date : 2024-11-07 DOI: 10.1093/jmicro/dfae051

Kousuke Ooe, Takehito Seki, Mitsuru Nogami, Yuichi Ikuhara, Naoya Shibata

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Improved Dopant Fraction Variance Estimation in Statistical ALCHEMI Based on Correct Error Propagation Rule. 基于正确的误差传播规则改进统计 ALCHEMI 中的掺杂分数方差估计。

Microscopy (Oxford, England) Pub Date : 2024-10-30 DOI: 10.1093/jmicro/dfae052

Akimitsu Ishizuka, Masahiro Ohtsuka, Shunsuke Muto

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Recent advancement and human tissue applications of volume electron microscopy. 体视电子显微镜的最新进展和人体组织应用。

Microscopy (Oxford, England) Pub Date : 2024-10-18 DOI: 10.1093/jmicro/dfae047

Makoto Abe, Nobuhiko Ohno

{"title":"Recent advancement and human tissue applications of volume electron microscopy.","authors":"Makoto Abe, Nobuhiko Ohno","doi":"10.1093/jmicro/dfae047","DOIUrl":"https://doi.org/10.1093/jmicro/dfae047","url":null,"abstract":"Structural observations are essential for the advancement of life science. Volume electron microscopy has recently realized remarkable progress in the three-dimensional analyses of biological specimens for elucidating complex ultrastructures in several fields of life science. The advancements in volume electron microscopy technologies have led to improvements, including higher resolution, more stability, and the ability to handle larger volumes. Although human applications of volume electron microscopy remain limited, the reported applications in various organs have already provided previously unrecognized features of human tissues and also novel insights of human diseases. Simultaneously, the application of volume electron microscopy to human studies faces challenges, including ethical and clinical hurdles, costs of data storage and analysis, and efficient and automated imaging methods for larger volume. Solutions including the use of residual clinical specimens and data analysis based on artificial intelligence would address those issues and establish the role of volume electron microscopy in human structural research. Future advancements in volume electron microscopy are anticipated to lead to transformative discoveries in basic research and clinical practice, deepening our understanding of human health and diseases for better diagnostic and therapeutic strategies.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acceptance characterization of electron detector in SEM using stainless steel sphere. 使用不锈钢球对扫描电子显微镜中的电子探测器进行验收表征。

Microscopy (Oxford, England) Pub Date : 2024-10-16 DOI: 10.1093/jmicro/dfae050

Takashi Sekiguchi, Yuanzhao Yao, Ryosuke Sonoda, Yasunari Sohda

{"title":"Acceptance characterization of electron detector in SEM using stainless steel sphere.","authors":"Takashi Sekiguchi, Yuanzhao Yao, Ryosuke Sonoda, Yasunari Sohda","doi":"10.1093/jmicro/dfae050","DOIUrl":"https://doi.org/10.1093/jmicro/dfae050","url":null,"abstract":"Although modern scanning electron microscope (SEM) possess several electron detectors, it is not clear what kind of information is contained in a SEM image taken by a certain detector. Especially the detectors installed in the objective lens are difficult to know their characters. Thus, we propose a simple method to assess the acceptance of electron detector using a stainless-steel sphere. After taking images under certain conditions, say electron beam energy, working distance etc., the image intensity of each pixel point, which is characterized by coordinate (θ, φ), is evaluated. The advantage of this method is the ease of implementation and the whole information of electron emission from the tilted surfaces is contained in the image. Using this information, the acceptance of the detector can be analyzed systematically. In this paper, the traditional Everhart-Thornley detector is analyzed with this method. It is demonstrated how the sphere image changes according to the measurement condition. The ET image quality is strongly governed by working distance but not so much by the electron beam energy. We propose an alternative method to avoid the ambiguity of working distance. Using a needle type specimen stage, the ET image does not vary so much with WD and the reliability of ET image significantly improves.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resolution Improvement of Differential Phase-Contrast Microscopy via Tilt-Series Acquisition for Environmental Cell Application. 通过倾斜序列采集提高环境细胞应用中的差分相位对比显微镜分辨率

Microscopy (Oxford, England) Pub Date : 2024-10-16 DOI: 10.1093/jmicro/dfae049

Kazutaka Mitsuishi, Fumiaki Ichihashi, Yoshio Takahashi, Katsuaki Nakazawa, Masaki Takeguchi, Ayako Hashimoto, Toshiaki Tanigaki

引用次数: 0

Anisotropic electronic structure study of MgB2C2 using soft X-ray emission 0spectroscopy microscopes. 利用软 X 射线发射光谱显微镜研究 MgB2C2 的各向异性电子结构。

Microscopy (Oxford, England) Pub Date : 2024-10-16 DOI: 10.1093/jmicro/dfae048

Yuki Hada, Masami Terauchi, Tomoya Saito, Yohei K Sato, Masaaki Baba, Masatoshi Takeda

{"title":"Anisotropic electronic structure study of MgB2C2 using soft X-ray emission 0spectroscopy microscopes.","authors":"Yuki Hada, Masami Terauchi, Tomoya Saito, Yohei K Sato, Masaaki Baba, Masatoshi Takeda","doi":"10.1093/jmicro/dfae048","DOIUrl":"https://doi.org/10.1093/jmicro/dfae048","url":null,"abstract":"The anisotropic electronic structure of MgB2C2 was studied using soft X-ray emission spectroscopy electron microscopes. MgB2C2 fragments were selected by examining C K-emission profiles. C and B K-emission and Mg L-emission spectra were obtained, revealing common and distinct structures that reflect the mixing of valence orbitals. Since the material is reported to have two-dimensional B-C honeycomb layers, the orientational dependence of these emission spectra was also examined. Experimental data were compared with theoretically calculated partial density of states of the valence bands of the material. The C K-emission profile showed an apparent orientational dependence, while the B K-emission exhibited minimal dependence. This difference originated from the different energy distributions of C-2pz and B-2pz components in the valence bands. The Mg L-emission intensity was very small, likely due to charge transfer from Mg atoms to B-N layers. The Mg L-emission profile showed a peak related to structures in C-K and B-K. An unexpected intensity was observed just above the valence bands, which also showed orientational dependence, possibly due to a small deviation from the ideal composition of Mg:B:C = 1:2:2.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple derivation of L-absorption spectra of 3d transition metal elements by the self-absorption effect observed in soft X-ray emission spectra. 通过软 X 射线发射光谱中观察到的自吸收效应简单推导出 3d 过渡金属元素的 L 吸收光谱。

Microscopy (Oxford, England) Pub Date : 2024-10-04 DOI: 10.1093/jmicro/dfae012

Masami Terauchi, Yohei K Sato, Takaomi D Yokoyama, Takanori Murano

引用次数: 0

Deciphering prefrontal circuits underlying stress and depression: exploring the potential of volume electron microscopy. 解密压力和抑郁的前额叶回路：探索容积电子显微镜的潜力

Microscopy (Oxford, England) Pub Date : 2024-10-04 DOI: 10.1093/jmicro/dfae036

Hirotaka Nagai

{"title":"Deciphering prefrontal circuits underlying stress and depression: exploring the potential of volume electron microscopy.","authors":"Hirotaka Nagai","doi":"10.1093/jmicro/dfae036","DOIUrl":"10.1093/jmicro/dfae036","url":null,"abstract":"Adapting to environmental changes and formulating behavioral strategies are central to the nervous system, with the prefrontal cortex being crucial. Chronic stress impacts this region, leading to disorders including major depression. This review discusses the roles for prefrontal cortex and the effects of stress, highlighting similarities and differences between human/primates and rodent brains. Notably, the rodent medial prefrontal cortex is analogous to the human subgenual anterior cingulate cortex in terms of emotional regulation, sharing similarities in cytoarchitecture and circuitry, while also performing cognitive functions similar to the human dorsolateral prefrontal cortex. It has been shown that chronic stress induces atrophic changes in the rodent mPFC, which mirrors the atrophy observed in the subgenual anterior cingulate cortex and dorsolateral prefrontal cortex of depression patients. However, the precise alterations in neural circuitry due to chronic stress are yet to be fully unraveled. The use of advanced imaging techniques, particularly volume electron microscopy, is emphasized as critical for the detailed examination of synaptic changes, providing a deeper understanding of stress and depression at the molecular, cellular and circuit levels. This approach offers invaluable insights into the alterations in neuronal circuits within the medial prefrontal cortex caused by chronic stress, significantly enriching our understanding of stress and depression pathologies.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":"391-404"},"PeriodicalIF":0.0,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141753477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0