Microscopy (Oxford, England)最新文献

Impact of Atomic Thermal Motion on the Ti L2,3-edge Fine Structure. 原子热运动对Ti L2三棱精细结构的影响

IF 1.9

Microscopy (Oxford, England) Pub Date : 2025-10-07 DOI: 10.1093/jmicro/dfaf042

Mitsutaka Haruta, I-Ching Lin, Takashi Nemoto, Hiroki Kurata

{"title":"Impact of Atomic Thermal Motion on the Ti L2,3-edge Fine Structure.","authors":"Mitsutaka Haruta, I-Ching Lin, Takashi Nemoto, Hiroki Kurata","doi":"10.1093/jmicro/dfaf042","DOIUrl":"https://doi.org/10.1093/jmicro/dfaf042","url":null,"abstract":"This review examines the effects of thermal vibrations on core-level excitation spectra, with a particular emphasis on the Ti L 2,3-edge spectra of cubic perovskite-type titanium oxides (SrTiO3 and PbTiO3). Based on combining scanning transmission electron microscopy energy-loss near-edge structure analyses with cluster-type crystal-field multiplet calculations, the influence of atomic thermal vibrations on the fine structure of the Ti L 2,3-edge is investigated, and it is demonstrated that the thermal vibration of oxygen atoms in cubic SrTiO3 can be estimated from the spectrum by fitting experimental and theoretical results. The same approach was extended to cubic PbTiO3 such that isotropic thermal vibrations were identified that relate to the difference in the transition to a low-temperature tetragonal phase. Although the present technique does not directly resolve phonon modes, it treats thermal factors as adjustable parameters, enabling the identification of subtle vibrational features even in materials already widely studied. Further investigation of the relationship between thermal vibrations and the fine structure of core-loss spectra could assist in elucidating certain material properties. This review explores the effects of thermal vibrations on Ti L 2,3-edge spectra of cubic perovskite oxides (SrTiO3, PbTiO3). Combining STEM-ELNES with crystal-field multiplet calculations, it shows that oxygen thermal vibrations can be estimated from spectral fitting, revealing subtle vibrational features and their relation to phase transitions.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying Layer-Specific Thicknesses in Porcine Large Intestine Using X-ray Microscopy. 用x射线显微镜定量猪大肠的层特异性厚度。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2025-10-01 DOI: 10.1093/jmicro/dfaf041

Gregory Hirst, Zachary Ross, Adam Rose, North Graff, Kennedy Campbell, Paul Reynolds, Benjamin Terry

{"title":"Quantifying Layer-Specific Thicknesses in Porcine Large Intestine Using X-ray Microscopy.","authors":"Gregory Hirst, Zachary Ross, Adam Rose, North Graff, Kennedy Campbell, Paul Reynolds, Benjamin Terry","doi":"10.1093/jmicro/dfaf041","DOIUrl":"https://doi.org/10.1093/jmicro/dfaf041","url":null,"abstract":"Accurate quantification of the individual layers of the intestinal wall is essential for biomechanical modeling and the development of gastrointestinal medical devices. Traditional microscopy techniques, though widely used, are limited by their two-dimensional nature and potential for tissue distortion due to complex sample preparation. This study evaluates X-ray microscopy (XRM) as a non-destructive, three-dimensional alternative for measuring the thicknesses of the four major layers of porcine large intestinal tissue: serosa, muscularis externa, submucosa, and mucosa. Using the ZEISS Xradia 620 Versa, XRM scans were compared to standard light microscopy. XRM successfully visualized all four layers and yielded thickness measurements that were consistent with those obtained via standard microscopy, despite natural biological variability. Notably, XRM scans allowed for 3D reconstructions of tissue vasculature and did not need extensive preparation or staining. These findings establish XRM as a powerful and practical method for morphological analysis of soft tissues and offer the first reported absolute layer thicknesses for each layer of porcine large intestinal tissue which can be used in layer-specific constitutive biomechanical models. This study compares measurements of intestinal tissue layers obtained using X-ray microscopy to those from traditional light microscopy. The results show that X-ray microscopy provides comparable data while offering the advantages of 3D imaging and minimal tissue preparation. (Figure 2).","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145202399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent Probes for Visualizing ion Dynamics in Bacteria: Current Tools and Future Perspectives. 荧光探针在细菌离子动力学可视化：目前的工具和未来的展望。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2025-09-29 DOI: 10.1093/jmicro/dfaf040

Yusuke V Morimoto

引用次数: 0

Myosin-Ie drives ruffle-edge lamellipodia formation and motility in A549 invasive lung cancer cells. 肌球蛋白- ie驱动A549浸润性肺癌细胞褶边板足的形成和运动。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2025-09-26 DOI: 10.1093/jmicro/dfaf039

Haruka Morishita, Katsuhisa Kawai, Ayaka Noda, Youhei Egami, Nobukazu Araki

{"title":"Myosin-Ie drives ruffle-edge lamellipodia formation and motility in A549 invasive lung cancer cells.","authors":"Haruka Morishita, Katsuhisa Kawai, Ayaka Noda, Youhei Egami, Nobukazu Araki","doi":"10.1093/jmicro/dfaf039","DOIUrl":"https://doi.org/10.1093/jmicro/dfaf039","url":null,"abstract":"Lamellipodia are generally defined as thin, sheet-like cell protrusions that constitute the actin cytoskeleton-based motile apparatus, which promotes the movement of migrating cells. Recently, we identified a novel type of lamellipodia, termed ruffle-edge lamellipodia, which have α-actinin-4 (ACTN4)-enriched multilayer membrane folds at their leading edges in certain invasive cancer cell lines. In this study, the role of unconventional myosin-Ie (Myo1E) in ACTN4-enriched ruffle-edge lamellipodia was analyzed using live-cell, immunofluorescence, and scanning electron microscopy. Immunofluorescence microscopy for endogenous Myo1E and live-cell imaging of mApple-Myo1E expressing cells showed that Myo1E was localized to ACTN4-rich lamellipodia tips in A549 cells. The wound healing assay and live-cell movies showed that Myo1E siRNA knockdown significantly suppressed cell migration and ruffle-edge lamellipodia formation. Furthermore, scanning electron microscopy demonstrated that Myo1E knockdown significantly reduced ruffle-edge structures. These results suggest that Myo1E may play an important role not only in the motility of ruffle-edge lamellipodia but also in the construction of ruffle-edge structures, which are probably associated with cancer cell invasion and metastasis.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data-driven ELNES/XANES analysis: predicting spectra, unveiling structures and quantifying properties. 数据驱动的ELNES/XANES分析：预测光谱，揭示结构和量化属性。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2025-09-11 DOI: 10.1093/jmicro/dfaf038

Teruyasu Mizoguchi

{"title":"Data-driven ELNES/XANES analysis: predicting spectra, unveiling structures and quantifying properties.","authors":"Teruyasu Mizoguchi","doi":"10.1093/jmicro/dfaf038","DOIUrl":"https://doi.org/10.1093/jmicro/dfaf038","url":null,"abstract":"Core-loss spectroscopies using electrons and X-rays, such as electron energy loss near-edge structures (ELNES) and X-ray absorption near-edge structures (XANES), are indispensable tools for materials characterization and development. These techniques provide detailed insights into atomic environments, chemical bonding, and vibrational properties that underpin material functionality. Traditionally, ELNES/XANES analyses have relied on qualitative interpretation or comparisons with reference spectra obtained from experiments and/or simulations. Recent advances in data-driven approaches, however, have enabled more quantitative and predictive use of these spectra. This review highlights newly developed data-driven methodologies that extend far beyond conventional ELNES/XANES analysis. These approaches accelerate ELNES/XANES simulations, enable the extraction of radial distribution functions, and quantify multiple material properties directly from spectral data. To enhance the interpretability of machine learning (ML) predictions, sensitivity analysis is employed to elucidate the relationships between specific spectral features and target properties. The rapid growth of open materials databases, coupled with increasingly powerful ML models, has further fueled these developments. Together, these advances would point to a future in which automated, interpretable and scalable spectroscopy serves as a central driver for deeper understandings and accelerated materials discovery.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-low accelerating voltage scanning electron microscopy with multiple imaging detectors-imaging and analysis at the 'sweet spot' - secondary publication. 超低加速电压扫描电子显微镜与多个成像探测器-成像和分析在“甜蜜点”-二次出版。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2025-09-10 DOI: 10.1093/jmicro/dfaf032

引用次数: 0

Characterization of ultra-variable-pressure detector for secondary electrons in low vacuum SEM. 低真空扫描电镜中二次电子超变压探测器的表征。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2025-09-04 DOI: 10.1093/jmicro/dfaf037

Yuanzhao Yao, Ryosuke Sonoda, Yasunari Sohda, Takashi Sekiguchi

{"title":"Characterization of ultra-variable-pressure detector for secondary electrons in low vacuum SEM.","authors":"Yuanzhao Yao, Ryosuke Sonoda, Yasunari Sohda, Takashi Sekiguchi","doi":"10.1093/jmicro/dfaf037","DOIUrl":"https://doi.org/10.1093/jmicro/dfaf037","url":null,"abstract":"Scanning electron microscope (SEM) observation in low vacuum can overcome the issue of charge-up at the specimen surface, allowing for the observation of insulating samples without sample pretreatment. The ultra-variable-pressure detector (UVD) was developed as a secondary electron (SE) detector for the low vacuum observation in SEM. It works by collecting the light signal released from the collision between secondary electrons and gas molecules. In this study, we propose a simple method using a stainless steel (SUS) sphere to characterize the feature of UVD signal in low vacuum SEM, and compare it with the traditional Everhart-Thornley (E-T) detector in normal SEM. The UVD signal showed characteristic features, namely a two-round-peak feature in the profile, which is different from that of E-T detector. By experiment and simulation, we revealed that at higher vacuum levels (as a few Pa), SEs provide the primary contribution to the UVD signal, exhibiting a profile similar to that of the E-T signal. As the vacuum deteriorates, as 30 Pa, the main contribution to the UVD signal shifts from SEs to low energy backscattered electrons (BSE). Our finding indicates that by tuning the chamber pressure, we can vary the UVD image between SE and low energy BSE features.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144994664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and electron microscopic study of thick cross-striated linear fibrils in mammalian cell nuclei. 哺乳动物细胞核中粗横纹线状原纤维的检测与电镜研究。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2025-08-28 DOI: 10.1093/jmicro/dfaf036

Mark I Mosevitsky

{"title":"Detection and electron microscopic study of thick cross-striated linear fibrils in mammalian cell nuclei.","authors":"Mark I Mosevitsky","doi":"10.1093/jmicro/dfaf036","DOIUrl":"https://doi.org/10.1093/jmicro/dfaf036","url":null,"abstract":"Using an electron microscope, thick (30-100 nm wide), linear (not branched), cross-striated protein fibrils with an axial repeat of about 65 nm were detected in mammalian cell nuclei. These fibrils differ from the thin filaments of the nuclear matrix described in the literature. Therefore, in this work, the main efforts were aimed at demonstrating the nuclear origin of thick fibrils. Their presence in the material of nuclei destroyed by ultrasound, their contact with isolated nucleoli, and their presence in residual nuclei (nuclear matrix) are shown. Contacts of thick fibrils with both chromatin and the network of filaments of the nuclear matrix were observed. Thick fibrils, which are axial components of condensed chromosomes, are preserved during mitosis. It is likely that their contacts with chromatin and elements of the nuclear matrix are also preserved, ensuring the reproduction of the internal structure of the nuclei in daughter cells. Thick fibrils disintegrate in a medium with low ionic strength. Perhaps this is the reason for their absence in other authors' nuclear matrix preparations. In this work, the nuclei were isolated, and all experiments were carried out in a \"complete medium \"simulating the intranuclear salt content. In the cell nuclei, thick (30-100 nm in diameter) linear cross-striated (axial repeat approximately 65 nm) fibrils were detected and described using an electron microscope. They are presumably components of the nuclear matrix.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144981609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Progress in Electron Energy Loss Spectroscopy with Concurrent Spatial and Momentum Resolution. 同步空间和动量分辨率的电子能量损失谱研究进展。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2025-08-28 DOI: 10.1093/jmicro/dfaf035

Lan Song, Ruilin Mao, Peng Gao

{"title":"Recent Progress in Electron Energy Loss Spectroscopy with Concurrent Spatial and Momentum Resolution.","authors":"Lan Song, Ruilin Mao, Peng Gao","doi":"10.1093/jmicro/dfaf035","DOIUrl":"https://doi.org/10.1093/jmicro/dfaf035","url":null,"abstract":"Scanning transmission electron microscopy-electron energy loss spectroscopy (STEM-EELS) has emerged as a state-of-the-art characterization modality in materials science, undergoing transformative advancements over the past decade. Revolutionary developments in monochromator technology have pushed EELS energy resolution into the sub-10 meV regime, enabling investigations of low-energy excitations such as phonons, excitons, plasmons, and polaritons at nanometer and sub-nanometer scales, in addition to traditional core-loss spectroscopy. Besides to the high spatial resolution and high energy resolution, the coherent nature of STEM electron probes now allows momentum-resolved spectral information to be acquired, providing an ideal platform for correlating nanoscale structural features with functional properties at the nanometer and atomic level. This review surveys recent breakthroughs in STEM-EELS methodology, with particular emphasis on the four-dimensional electron energy loss spectroscopy (4D-EELS) technique, which simultaneously captures spectral information across spatial, momentum, and energy dimensions with unprecedented efficiency. We highlight landmark scientific discoveries enabled by this spontaneous spatial-momentum resolving capability, including phonon dispersion mapping, plasmon dispersion mapping, and magnon mapping. The review concludes with perspectives on future technical refinements, such as resolution enhancements, machine learning-driven data analytics, and in-situ characterization capabilities, and the potential of this technology to revolutionize interdisciplinary research in quantum materials and nanophotonics. This review methodically investigates recent breakthroughs in low-loss excitation studies using STEM-EELS with a primary focus on phonon dynamics. Furthermore, we introduce the recently developed 4D-EELS Technique adopting parallel acquisition of spectral information across spatial, momentum, and energy dimensions.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144981566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Encapsulation, vacuolation and phagocytosis of the opportunistic fungal pathogen Cryptococcus in the liver of an immunocompetent host. 机会性真菌病原体隐球菌在免疫能力强的宿主肝脏中的包封、空泡化和吞噬作用。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2025-08-01 DOI: 10.1093/jmicro/dfaf003

Chul Jong Yoon, Je Geun Chi, Ki Woo Kim

{"title":"Encapsulation, vacuolation and phagocytosis of the opportunistic fungal pathogen Cryptococcus in the liver of an immunocompetent host.","authors":"Chul Jong Yoon, Je Geun Chi, Ki Woo Kim","doi":"10.1093/jmicro/dfaf003","DOIUrl":"10.1093/jmicro/dfaf003","url":null,"abstract":"The cellular characteristics of the opportunistic fungal pathogen Cryptococcus species were investigated in the infected liver of an immunocompetent host using transmission electron microscopy (TEM). With no records of immunodeficiency, the 3-year-old female patient displayed a high-grade fever, lethargy and increasing jaundice. TEM analysis revealed the presence of round yeast cells in the patient's liver. These fungal yeast cells exhibited an array of cellular events in the host's liver: (i) the formation of polysaccharide capsules outside the cell wall, (ii) vacuolation in the cytoplasm and (iii) phagocytosis by Kupffer cells. The yeast cells were surrounded by electron-transparent polysaccharide capsules (approximately 5 μm thick). A series of yeast vacuolations were observed at different stages of cell development. As vacuoles occupied the cytoplasm of yeast cells, the polysaccharide capsules were thinner and more electron-dense than those of intact yeast cells. Certain yeast cells were phagocytosed by Kupffer cells through the budding scars or discontinued regions in the cell walls. These observations suggested that the patient was suffering from liver cryptococcosis. This study provides insights into the behavior of opportunistic fungal pathogens in the livers of immunocompetent patients.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":"286-292"},"PeriodicalIF":1.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0