Microscopy (Oxford, England)最新文献

Applications of Secondary and Backscattered Electron Spectroscopic Imaging and a Proposal for New SEM Image Nomenclature. 二次散射和背散射电子能谱成像的应用及新电子能谱图像命名的建议。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2026-10-21 DOI: 10.1093/jmicro/dfag037

Takashi Sekiguchi, Yuanzhao Yao

{"title":"Applications of Secondary and Backscattered Electron Spectroscopic Imaging and a Proposal for New SEM Image Nomenclature.","authors":"Takashi Sekiguchi, Yuanzhao Yao","doi":"10.1093/jmicro/dfag037","DOIUrl":"https://doi.org/10.1093/jmicro/dfag037","url":null,"abstract":"With the increasing diversity of SEM detectors today, classification based solely on secondary and backscattered electrons is no longer sufficient; there is a growing need to specify energy and emission direction when discussing image information. In particular, for detectors capable of energy discrimination, an appropriate method for classifying images is required. We propose a nomenclature for classifying images by explicitly indicating energy, emission direction, and detector position following the abbreviations \"SE\" and \"BE.\" Energy is displayed as three bands - A, B, and C- defined by the ends of the spectrum on a logarithmic axis, while emission direction is divided into regions 1, 2, and 3 in 30° increments from the zenith angle. Detectors are designated with \"o\" or \"d\" depending on whether they are circularly symmetric or directional with respect to the primary electron beam. For example, they are denoted as SE/A2o or BE/AB3d. Using this notation, we classify images obtained with low-pass secondary electron detectors (Fountain detector) and high-pass backscattered electron detectors, and discuss their characteristics.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149231336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological study of the anterior cruciate ligament and its tibial insertion in the human knee joint. 人膝关节前交叉韧带及其胫骨止点的形态学研究。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2026-10-19 DOI: 10.1093/jmicro/dfag036

Yutaro Shibuta, Tatsuo Shimada, Tsuguaki Hosoyama, Masashi Hirakawa, Nobuhiro Kaku

{"title":"Morphological study of the anterior cruciate ligament and its tibial insertion in the human knee joint.","authors":"Yutaro Shibuta, Tatsuo Shimada, Tsuguaki Hosoyama, Masashi Hirakawa, Nobuhiro Kaku","doi":"10.1093/jmicro/dfag036","DOIUrl":"https://doi.org/10.1093/jmicro/dfag036","url":null,"abstract":"Purpose: We investigated whether ligament-derived collagen fibrils traversing the cartilage/fibrocartilage region toward the tibial bone could be visualized using SEM.Methods: ACL specimens were obtained from four patients with ACL rupture (aged 16-49 years) and three older adults (aged 60-75 years) with osteoarthritis. Specimens were fixed and examined under light microscopy (LM; sections stained with light green and aldehyde fuchsin), transmission electron microscopy (TEM; modified tannic acid method), and scanning electron microscopy (SEM; NaOH-treated blocks).Results: The central portion of the ACL consisted of parallel-arranged collagen fibers, with thread-like elastic fibers stained by aldehyde fuchsin sparsely distributed among them. SEM following NaOH treatment revealed collagen fibrils (diameter ∼120-140 nm). Elastic fibers were also scattered among collagen bundles on SEM. On TEM, electron-dense structures consistent with elastic fibers (diameter ∼600 nm) were identified. At the tibial insertion, LM demonstrated that the ACL branched within the cartilage and attached to the tibia. SEM enabled discrimination between the bundled ACL collagen fibrils and the finer collagen fibrils (diameter ∼80 nm) surrounding chondrocytes.Conclusion: We histologically demonstrated that branches of the human ACL pass through the joint and attach to the tibia. Ligament-derived collagen fibrils were observed branching and descending through the cartilage/fibrocartilage region toward the tibial bone.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149225541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extending the Computable Depth Range in Electron Ptychography using Event-Converted Data. 利用事件转换数据扩展电子平面图的可计算深度范围。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2026-10-13 DOI: 10.1093/jmicro/dfag035

Kazutaka Mitsuishi, Masahiko Shimizu, Katsuaki Nakazawa, Masaki Takeguchi, Ayako Hashimoto, Toshiaki Tanigaki

引用次数: 0

Systematic study of chemical bonding states of lithium binary compounds from Li K-L emission spectra obtained by a VUV spectrometer mounted on a scanning electron microscope. 利用安装在扫描电子显微镜上的VUV光谱仪获得的Li K-L发射光谱，系统地研究了锂二元化合物的化学键态。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2026-10-01 DOI: 10.1093/jmicro/dfag017

Takanori Murano, Shogo Koshiya

引用次数: 0

Teriparatide reduces spinal neuroinflammatory phenotypes in ovariectomized rats revealed by an artificial intelligence-driven glial cell morphometry of bright-field tissue sections. 人工智能驱动的亮场组织切片胶质细胞形态测定显示，特利帕肽可减少卵巢切除大鼠的脊髓神经炎症表型。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2026-10-01 DOI: 10.1093/jmicro/dfag018

Shinnosuke Nishimoto, Haruhisa Watanabe, Marie Hoshi-Numahata, Atsuko Nakanishi-Kimura, Tomoya Tanaka, Aya Takakura, Ryoko Takao-Kawabata, Yoshiaki Sato, Tadahiro Iimura

{"title":"Teriparatide reduces spinal neuroinflammatory phenotypes in ovariectomized rats revealed by an artificial intelligence-driven glial cell morphometry of bright-field tissue sections.","authors":"Shinnosuke Nishimoto, Haruhisa Watanabe, Marie Hoshi-Numahata, Atsuko Nakanishi-Kimura, Tomoya Tanaka, Aya Takakura, Ryoko Takao-Kawabata, Yoshiaki Sato, Tadahiro Iimura","doi":"10.1093/jmicro/dfag018","DOIUrl":"10.1093/jmicro/dfag018","url":null,"abstract":"Osteoporosis frequently presents with lower back pain, often accompanied by hypersensitivity, even in the absence of vertebral fractures, suggesting the involvement of central neuroinflammatory mechanisms. The activation of spinal glia has been implicated as a key driver of pain. Clinical and preclinical studies have also shown that teriparatide (TPTD), a bone anabolic drug, alleviates osteoporotic pain and raises the possibility of anti-neuroinflammatory effects. We previously reported that TPTD suppresses neuroinflammatory microglial proliferation in the spinal dorsal horn of an ovariectomized (OVX) rat model of postmenopausal osteoporosis. To further substantiate these previous findings, this study morphometrically investigated neuroinflammatory alterations in the same patho-pharmacological setting by establishing an artificial intelligence (AI)-driven morphometric pipeline applied to 3,3'-diaminobenzidine (DAB)-stained bright-field images. OVX increased the number of microglia, induced process shortening and higher circularity and expanded glial fibrillary acidic protein (GFAP)-positive astrocytic areas. TPTD partially attenuated OVX-induced glial changes. These findings indicate that OVX induces spinal neuroinflammation involving both microglia and astrocytes and that TPTD mitigates these neuroinflammatory responses. Moreover, the combination of 2D bright-field imaging and AI-driven morphometry represents a practical, accessible approach that requires minimal specialized equipment, yet sensitively captures OVX- and TPTD-induced microglial alterations and enables phenotype-based classification.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":"377-389"},"PeriodicalIF":1.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13652547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147494742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Partial coherence of inelastically scattered electrons in transmission electron microscopy. 透射电镜中非弹性散射电子的部分相干性。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2026-10-01 DOI: 10.1093/jmicro/dfag020

Budhika Gehan Mendis

引用次数: 0

Structural optimization of model sample for high-resolution soft/hard interface analysis. 用于高分辨率软/硬界面分析的模型样品结构优化。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2026-10-01 DOI: 10.1093/jmicro/dfag015

Kaname Yoshida, Hsin-Hui Huang, Tomohiro Miyata, Yohei K Sato, Hiroshi Jinnai

引用次数: 0

Fluorescent protein-based probe architecture modulates rotational and translational diffusion readouts in polarization-dependent fluorescence correlation spectroscopy. 荧光蛋白为基础的探针结构调节旋转和平移扩散读数在偏振依赖的荧光相关光谱。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2026-10-01 DOI: 10.1093/jmicro/dfag021

Miho Hanaoka, Kenta Saito, Masahiko Kawagishi, Johtaro Yamamoto, Nori Nakai, Minori Iida, Keisuke Sato, Makoto Nakakido, Satoru Nagatoishi, Kouhei Tsumoto, Masataka Kinjo, Sumio Terada

{"title":"Fluorescent protein-based probe architecture modulates rotational and translational diffusion readouts in polarization-dependent fluorescence correlation spectroscopy.","authors":"Miho Hanaoka, Kenta Saito, Masahiko Kawagishi, Johtaro Yamamoto, Nori Nakai, Minori Iida, Keisuke Sato, Makoto Nakakido, Satoru Nagatoishi, Kouhei Tsumoto, Masataka Kinjo, Sumio Terada","doi":"10.1093/jmicro/dfag021","DOIUrl":"10.1093/jmicro/dfag021","url":null,"abstract":"Polarization-dependent fluorescence correlation spectroscopy (Pol-FCS) simultaneously measures translational and rotational diffusion of fluorescently labeled molecules and provides information on molecular size and shape. Rotational diffusion is, in principle, expected to provide even higher sensitivity than translational diffusion, which is routinely used in conventional FCS to detect binding of fluorescent protein (FP)-fused binders to their targets. However, when FPs are fused to binders via flexible linkers, local wobbling of the FP can decouple its rotational diffusion from that of the binding complex. Quantitative evaluation of this decoupling effect remains lacking. Here, leveraging our expertise from polarization-dependent orientation probes, POLArIS, we designed and compared two classes of probes, termed 'Rigid' and 'Flex', that differ in how tightly the FP is linked to the anti-ALFAtag nanobody, to evaluate how probe architecture affects Pol-FCS readouts. Using ALFAtag and the rod-like repeat protein DHR10 as a structurally anisotropic binding target, we varied probe binding geometry while keeping molecular weight nearly constant and measured rotational and translational diffusion. Flex probes exhibited shorter rotational diffusion times than Rigid probes when bound to the same targets, consistent with the expected local reorientation. Notably, translational diffusion times also showed architecture-dependent differences between Rigid and Flex probes. These results demonstrate that probe architecture is a key determinant of diffusion readouts in FP-based Pol-FCS and suggest practical probe design principles for sensitive reporters of intermolecular binding accompanied by changes in complex shape.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":"398-411"},"PeriodicalIF":1.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147640825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wide-field montage on transmission electron microscopy via network tele-microscopy enables quantitative analysis of mouse olfactory bulb neural circuits. 通过网络远程显微镜对透射电子显微镜进行宽视场蒙太奇，可以对小鼠嗅球神经回路进行定量分析。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2026-10-01 DOI: 10.1093/jmicro/dfag022

Haruyo Yamanishi, Emi Kiyokage, Satoshi Ichikawa, Kaoru Mitsuoka, Kazunori Toida

{"title":"Wide-field montage on transmission electron microscopy via network tele-microscopy enables quantitative analysis of mouse olfactory bulb neural circuits.","authors":"Haruyo Yamanishi, Emi Kiyokage, Satoshi Ichikawa, Kaoru Mitsuoka, Kazunori Toida","doi":"10.1093/jmicro/dfag022","DOIUrl":"10.1093/jmicro/dfag022","url":null,"abstract":"Transmission electron microscopy (TEM) provides nanometer-scale resolution, which is essential for ultrastructural analysis of biological tissue. However, its application for large tissue areas is limited by a restricted field of view and observer-dependent sampling. In this study, we present a workflow that combines wide-field montage TEM-via 'network tele-microscopy'-with correlative light and electron microscopy to enable large-area ultrastructural analysis while preserving synapse-level resolution. We demonstrate this approach in the glomerular layer and tyrosine hydroxylase-positive neurons in mouse olfactory bulb glomeruli, which exhibit dense, heterogeneous synaptic organization. Light and confocal laser scanning microscopy were first used for orientation in regions of interest, allowing the generation of continuous wide-field montage TEM datasets. This approach allowed systematic identification and quantification of synapses across an entire glomerulus while maintaining spatial relationships among ultrastructural elements. This study demonstrates a technically feasible platform for the development of TEM and the future integration of network tele-microscopy with computational methods.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":"412-424"},"PeriodicalIF":1.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147679155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low‑damage thinning of polymer specimens for transmission electron microscopy by gas cluster ion beam irradiation. 气体束离子束辐照用于透射电子显微镜的聚合物样品的低损伤减薄。

IF 1.9

Microscopy (Oxford, England) Pub Date : 2026-10-01 DOI: 10.1093/jmicro/dfag023

Shin Inamoto, Akiyo Yoshida, Mayumi Oto, Kazuhiro Matsuda, Yuji Otsuka

引用次数: 0