A wide variety of techniques for a volume electron microscopy.

Abstract

Electron microscopy (EM) is known to be the only research equipment able to resolve the ultrastructure of cells, including intracellular organelles and synapses. Researchers studying the brain connectome have re-evaluated the value of EM. The development of new EM techniques and tools has been active in these two decades. In this review, based on these trends, currently available EM tools and recently developing new techniques are introduced.