npj Microgravity最新文献_第3页

Discoveries from human stem cell research in space that are relevant to advancing cellular therapies on Earth. 从太空人类干细胞研究中发现与推动地球细胞疗法有关的发现。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2024-08-21 DOI: 10.1038/s41526-024-00425-0

Fay Ghani, Abba C Zubair

引用次数: 0

Author Correction: Hydrogel mechanical properties in altered gravity. 作者更正：改变重力下的水凝胶机械特性。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2024-08-21 DOI: 10.1038/s41526-024-00426-z

Vanja Mišković, Immacolata Greco, Christophe Minetti, Francesca Cialdai, Monica Monici, Arianna Gazzi, Jeremiah Marcellino, Yarjan Abdul Samad, Lucia Gemma Delogu, Andrea C Ferrari, Carlo Saverio Iorio

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Functional activities essential for space exploration performed in partial gravity during parabolic flight. 在抛物线飞行过程中，在部分重力状态下进行空间探索所必需的功能活动。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2024-08-17 DOI: 10.1038/s41526-024-00422-3

Gilles Clément, Timothy R Macaulay, Austin Bollinger, Hannah Weiss, Scott J Wood

引用次数: 0

Simulated microgravity impairs human NK cell cytotoxic activity against space radiation-relevant leukemic cells. 模拟微重力会损害人类 NK 细胞对太空辐射相关白血病细胞的细胞毒活性。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2024-08-14 DOI: 10.1038/s41526-024-00424-1

Bradford M Kuhlman, Jonathan H Diaz, Trang Simon, Kimberly D Reeves, Stephen J Walker, Anthony Atala, Graça Almeida-Porada, Christopher D Porada

{"title":"Simulated microgravity impairs human NK cell cytotoxic activity against space radiation-relevant leukemic cells.","authors":"Bradford M Kuhlman, Jonathan H Diaz, Trang Simon, Kimberly D Reeves, Stephen J Walker, Anthony Atala, Graça Almeida-Porada, Christopher D Porada","doi":"10.1038/s41526-024-00424-1","DOIUrl":"10.1038/s41526-024-00424-1","url":null,"abstract":"Natural killer (NK) cells are an important first-line of defense against malignant cells. Because of the potential for increased cancer risk from astronaut exposure to space radiation, we determined whether microgravity present during spaceflight affects the body's defenses against leukemogenesis. Human NK cells were cultured for 48 h under normal gravity and simulated microgravity (sμG), and cytotoxicity against K-562 (CML) and MOLT-4 (T-ALL) cells was measured using standard methodology or under continuous sμG. This brief exposure to sμG markedly reduced NK cytotoxicity against both leukemias, and these deleterious effects were more pronounced in continuous sμG. RNA-seq performed on NK cells from two additional healthy donors provided insight into the mechanism(s) by which sμG reduced cytotoxicity. Given our prior report of space radiation-induced human T-ALL in vivo, the reduced cytotoxicity against MOLT-4 is striking and raises the possibility that μG may increase astronaut risk of leukemogenesis during prolonged missions beyond LEO.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"10 1","pages":"85"},"PeriodicalIF":4.4,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11324864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141983920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing a method for the cryopreservation of viable peripheral blood mononuclear cells in the International Space Station. 建立在国际空间站冷冻保存有活力的外周血单核细胞的方法。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2024-08-09 DOI: 10.1038/s41526-024-00423-2

Hiroto Ishii, Rin Endo, Sanae Hamanaka, Nobuyuki Hidaka, Maki Miyauchi, Naho Hagiwara, Takahisa Miyao, Tohru Yamamori, Tatsuya Aiba, Nobuko Akiyama, Taishin Akiyama

{"title":"Establishing a method for the cryopreservation of viable peripheral blood mononuclear cells in the International Space Station.","authors":"Hiroto Ishii, Rin Endo, Sanae Hamanaka, Nobuyuki Hidaka, Maki Miyauchi, Naho Hagiwara, Takahisa Miyao, Tohru Yamamori, Tatsuya Aiba, Nobuko Akiyama, Taishin Akiyama","doi":"10.1038/s41526-024-00423-2","DOIUrl":"10.1038/s41526-024-00423-2","url":null,"abstract":"The analysis of cells frozen within the International Space Station (ISS) will provide crucial insights into the impact of the space environment on cellular functions and properties. The objective of this study was to develop a method for cryopreserving blood cells under the specific constraints of the ISS. In a ground experiment, mouse blood was directly mixed with a cryoprotectant and gradually frozen at -80 °C. Thawing the frozen blood sample resulted in the successful recovery of viable mononuclear cells when using a mixed solution of dimethylsulfoxide and hydroxyethyl starch as a cryoprotectant. In addition, we developed new freezing cases to minimize storage space utilization within the ISS freezer. Finally, we confirmed the recovery of major mononuclear immune cell subsets from the cryopreserved blood cells through a high dimensional analysis of flow cytometric data using 13 cell surface markers. Consequently, this ground study lays the foundation for the cryopreservation of viable blood cells on the ISS, enabling their analysis upon return to Earth. The application of this method in ISS studies will contribute to understanding the impact of space environments on human cells. Moreover, this method may find application in the cryopreservation of blood cells in situations where research facilities are inadequate.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"10 1","pages":"84"},"PeriodicalIF":4.4,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11315897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141914569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogel mechanical properties in altered gravity. 改变重力条件下的水凝胶机械特性。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2024-08-08 DOI: 10.1038/s41526-024-00388-2

Vanja Mišković, Immacolata Greco, Christophe Minetti, Francesca Cialdai, Monica Monici, Arianna Gazzi, Jeremiah Marcellino, Yarjan Abdul Samad, Lucia Gemma Delogu, Andrea C Ferrari, Carlo Saverio Iorio

引用次数: 0

Light has a principal role in the Arabidopsis transcriptomic response to the spaceflight environment. 光在拟南芥转录组对太空飞行环境的反应中起着主要作用。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2024-08-06 DOI: 10.1038/s41526-024-00417-0

Mingqi Zhou, Robert J Ferl, Anna-Lisa Paul

{"title":"Light has a principal role in the Arabidopsis transcriptomic response to the spaceflight environment.","authors":"Mingqi Zhou, Robert J Ferl, Anna-Lisa Paul","doi":"10.1038/s41526-024-00417-0","DOIUrl":"10.1038/s41526-024-00417-0","url":null,"abstract":"The Characterizing Arabidopsis Root Attractions (CARA) spaceflight experiment provides comparative transcriptome analyses of plants grown in both light and dark conditions within the same spaceflight. CARA compared three genotypes of Arabidopsis grown in ambient light and in the dark on board the International Space Station (ISS); Col-0, Ws, and phyD, a phytochrome D mutant in the Col-0 background. In all genotypes, leaves responded to spaceflight with a higher number of differentially expressed genes (DEGs) than root tips, and each genotype displayed distinct light / dark transcriptomic patterns that were unique to the spaceflight environment. The Col-0 leaves exhibited a substantial dichotomy, with ten-times as many spaceflight DEGs exhibited in light-grown plants versus dark-grown plants. Although the total number of DEGs in phyD leaves is not very different from Col-0, phyD altered the manner in which light-grown leaves respond to spaceflight, and many genes associated with the physiological adaptation of Col-0 to spaceflight were not represented. This result is in contrast to root tips, where a previous CARA study showed that phyD substantially reduced the number of DEGs. There were few DEGs, but a series of space-altered gene categories, common to genotypes and lighting conditions. This commonality indicates that key spaceflight genes are associated with signal transduction for light, defense, and oxidative stress responses. However, these key signaling pathways enriched from DEGs showed opposite regulatory direction in response to spaceflight under light and dark conditions, suggesting a complex interaction between light as a signal, and light-signaling genes in acclimation to spaceflight.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"10 1","pages":"82"},"PeriodicalIF":4.4,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11303767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141898913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rotational and translational motions in a homogeneously cooling granular gas. 均匀冷却颗粒气体中的旋转和平移运动。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2024-07-31 DOI: 10.1038/s41526-024-00420-5

Torsten Trittel, Dmitry Puzyrev, Kirsten Harth, Ralf Stannarius

引用次数: 0

Growth and mineralization of fetal mouse long bones under microgravity and daily 1 g gravity exposure. 胎鼠长骨在微重力和每日 1 克重力暴露下的生长和矿化。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2024-07-27 DOI: 10.1038/s41526-024-00421-4

Jack J W A van Loon, Olga P Berezovska, Theodorus J M Bervoets, Dina Montufar-Solis, Cor M Semeins, Behrouz Zandieh-Doulabi, P Natalia V Rodionova, Jackie Duke, J Paul Veldhuijzen

{"title":"Growth and mineralization of fetal mouse long bones under microgravity and daily 1 g gravity exposure.","authors":"Jack J W A van Loon, Olga P Berezovska, Theodorus J M Bervoets, Dina Montufar-Solis, Cor M Semeins, Behrouz Zandieh-Doulabi, P Natalia V Rodionova, Jackie Duke, J Paul Veldhuijzen","doi":"10.1038/s41526-024-00421-4","DOIUrl":"10.1038/s41526-024-00421-4","url":null,"abstract":"In a previous Space Shuttle/Spacelab experiment (STS-42), we observed direct responses of isolated fetal mouse long bones to near weightlessness. This paper aimed to verify those results and study the effects of daily 1×g exposure during microgravity on the growth and mineralization of these bones. Two experiments were conducted: one on an American Space Shuttle mission (IML-2 on STS-65) and another on a Russian Bio-Cosmos flight (Bion-10 on Cosmos-2229). Despite differences in hardware, both used 17-day-old fetal mouse metatarsals cultured for 4 days. Results showed reduced proteoglycan content under microgravity compared to 1×g conditions, with no main differences in other cellular structures. While the overall metatarsal length was unaffected, the length increase of the mineralized diaphysis was significantly reduced under microgravity. Daily 1×g exposure for at least 6 h abolished the microgravity-induced reduction in cartilage mineralization, indicating the need for long-duration exposure to 1×g as an in-flight countermeasure using artificial gravity.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"10 1","pages":"80"},"PeriodicalIF":4.4,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11282293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141768076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing insights into microgravity induced muscle changes using Caenorhabditis elegans as a model organism. 以秀丽隐杆线虫为模式生物，推进对微重力诱发的肌肉变化的深入研究。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2024-07-26 DOI: 10.1038/s41526-024-00418-z

Laura J Beckett, Philip M Williams, Li Shean Toh, Volker Hessel, Lukas Gerstweiler, Ian Fisk, Luis Toronjo-Urquiza, Veeren M Chauhan

{"title":"Advancing insights into microgravity induced muscle changes using Caenorhabditis elegans as a model organism.","authors":"Laura J Beckett, Philip M Williams, Li Shean Toh, Volker Hessel, Lukas Gerstweiler, Ian Fisk, Luis Toronjo-Urquiza, Veeren M Chauhan","doi":"10.1038/s41526-024-00418-z","DOIUrl":"10.1038/s41526-024-00418-z","url":null,"abstract":"Spaceflight presents significant challenges to the physiological state of living organisms. This can be due to the microgravity environment experienced during long-term space missions, resulting in alterations in muscle structure and function, such as atrophy. However, a comprehensive understanding of the adaptive mechanisms of biological systems is required to devise potential solutions and therapeutic approaches for adapting to spaceflight conditions. This review examines the current understanding of the challenges posed by spaceflight on physiological changes, alterations in metabolism, dysregulation of pathways and the suitability and advantages of using the model organism Caenorhabditis elegans nematodes to study the effects of spaceflight. Research has shown that changes in the gene and protein composition of nematodes significantly occur across various larval stages and rearing environments, including both microgravity and Earth gravity settings, often mirroring changes observed in astronauts. Additionally, the review explores significant insights into the fundamental metabolic changes associated with muscle atrophy and growth, which could lead to the development of diagnostic biomarkers and innovative techniques to prevent and counteract muscle atrophy. These insights not only advance our understanding of microgravity-induced muscle atrophy but also lay the groundwork for the development of targeted interventions to mitigate its effects in the future.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"10 1","pages":"79"},"PeriodicalIF":4.4,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11282318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141768075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0