npj Microgravity最新文献_第2页

The mechanosensitive channel ELKIN1 regulates cellular adaptations to simulated microgravity. 机械敏感通道ELKIN1调节细胞对模拟微重力的适应。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-03-16 DOI: 10.1038/s41526-025-00466-z

Daphne Wang, Giulia Silvani, Lioba Schroeter, Remi Brynn, Joshua Chou, Kate Poole

{"title":"The mechanosensitive channel ELKIN1 regulates cellular adaptations to simulated microgravity.","authors":"Daphne Wang, Giulia Silvani, Lioba Schroeter, Remi Brynn, Joshua Chou, Kate Poole","doi":"10.1038/s41526-025-00466-z","DOIUrl":"10.1038/s41526-025-00466-z","url":null,"abstract":"In conditions of microgravity the human body undergoes extensive alterations in physiological function. However, it has proven challenging to determine how these changes are mediated at the molecular and cellular level. Here, we investigated whether ELKIN1, a mechanically activated ion channel, regulates changes in cellular and molecular structures in conditions of simulated microgravity. Deletion of ELKIN1 inhibited the simulated microgravity-induced alterations of cellular structure and attachment. In addition, cells lacking ELKIN1 did not exhibit changes in focal adhesion structures and redistribution of the YAP1 transcription factor in response to simulated microgravity, consistent with wild type cells. Finally, melanoma cell invasion of a collagen gel, from organotypic spheroids, was reduced in simulated microgravity, in an ELKIN1 dependent manner. Thus, the force sensing molecule, ELKIN1, modulates the impact of microgravity at both the molecular and cellular levels, revealing one of the molecular mechanisms that underpins cellular adaptations to conditions of microgravity.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"10"},"PeriodicalIF":4.4,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRPML1 ion channel promotes HepaRG cell differentiation under simulated microgravity conditions. TRPML1离子通道促进模拟微重力条件下HepaRG细胞的分化。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-03-15 DOI: 10.1038/s41526-025-00461-4

Huancai Fan, Dongyuan Lü, Zheng Lu, Hangyu Li, Zijuan Qi, Shujin Sun, Dongshi Guan, Mian Long, Ming Gao, Sijin Liu

{"title":"TRPML1 ion channel promotes HepaRG cell differentiation under simulated microgravity conditions.","authors":"Huancai Fan, Dongyuan Lü, Zheng Lu, Hangyu Li, Zijuan Qi, Shujin Sun, Dongshi Guan, Mian Long, Ming Gao, Sijin Liu","doi":"10.1038/s41526-025-00461-4","DOIUrl":"10.1038/s41526-025-00461-4","url":null,"abstract":"Stem cell differentiation must be regulated by intricate and complex interactions between cells and their surrounding environment, ensuring normal organ and tissue morphology such as the liver1. Though it is well acknowledged that microgravity provides necessary mechanical force signals for cell fate2, how microgravity affects growth, differentiation, and communication is still largely unknown due to the lack of real experimental scenarios and reproducibility tools. Here, Rotating Flat Chamber (RFC) was used to simulate ground-based microgravity effects to study how microgravity effects affect the differentiation of HepaRG (hepatic progenitor cells) cells. Unexpectedly, the results show that RFC conditions could promote HepaRG cell differentiation which exhibited increased expression of Alpha-fetoprotein (AFP), albumin (ALB), and Recombinant Cytokeratin 18 (CK18). Through screening a series of mechanical receptors, the ion channel TRPML1 was critical for promoting the differentiation effect under RFC conditions. Once TRPML1 was activated by stimulated microgravity effects, the concentration of lysosomal calcium ions was increased to activate the Wnt/β-catenin signaling pathway, which finally led to enhanced cell differentiation of HepaRG cells. In addition, the cytoskeleton was remodeled under RFC conditions to influence the expression of PI (3,5) P2, which is the best-known activator of TRPML1. In summary, our findings have established a mechanism by which simulated microgravity promotes the differentiation of HepaRG cells through the TRPML1 signaling pathway, which provides a potential target for the regulation of hepatic stem/progenitor cell differentiation and embryonic liver development under real microgravity conditions.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"9"},"PeriodicalIF":4.4,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Management of corneal injuries in spaceflight and recommendations for planetary missions. 航天飞行中角膜损伤的处理和行星任务的建议。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-03-11 DOI: 10.1038/s41526-025-00462-3

Alex Suh, Joshua Ong, Ethan Waisberg, John Berdahl, Andrew G Lee

引用次数: 0

The utility of animal models to inform the next generation of human space exploration. 利用动物模型为下一代人类太空探索提供信息。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-02-22 DOI: 10.1038/s41526-025-00460-5

Isla Duporge, Talmo Pereira, Santiago Castiello de Obeso, Julius G Bright Ross, Stephen J Lee, Allyson G Hindle

引用次数: 0

Plyometric training increases thickness and volume of knee articular cartilage in mice. 增强训练增加小鼠膝关节软骨的厚度和体积。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-02-13 DOI: 10.1038/s41526-025-00458-z

Marco Chiaberge, Neelima Thottappillil, Anna-Maria Liphardt, Anderson Furlanetto, Dylan Odell, Christine Wang, Stephen Hope, Stephen Smee, Joseph Rehfus, Anja Niehoff, Mark Shelhamer, Colin Norman, Marc J Philippon, Johnny Huard, Aaron W James, Chen-Ming Fan

{"title":"Plyometric training increases thickness and volume of knee articular cartilage in mice.","authors":"Marco Chiaberge, Neelima Thottappillil, Anna-Maria Liphardt, Anderson Furlanetto, Dylan Odell, Christine Wang, Stephen Hope, Stephen Smee, Joseph Rehfus, Anja Niehoff, Mark Shelhamer, Colin Norman, Marc J Philippon, Johnny Huard, Aaron W James, Chen-Ming Fan","doi":"10.1038/s41526-025-00458-z","DOIUrl":"10.1038/s41526-025-00458-z","url":null,"abstract":"Degeneration and thinning of articular cartilage lead to osteoarthritis and may result from reduced joint loading during e.g. bed rest or as a result of microgravity during space flight. Anabolic physical exercises for cartilage are not well studied to date. We built an experimental apparatus for plyometric training with mice to test potential benefits of jumping for articular cartilage. The exercise group (JUMP) performed jump training for 9 weeks and was compared with sedentary mice (control, CON) and hindlimb-suspended (HLS) mice (to simulate reduced loading) for the same duration. Knee cartilage was assessed via 3-dimensional reconstruction of micro-CT scans and histology. We observed significant thinning and volume reduction of articular cartilage at the medial tibial-femoral point of contact in the HLS group. Clustering of chondrocytes was present in HLS. By contrast, the JUMP group showed both cartilage thickening and volume increase. We observed a similar trend on trabecular bone thickness and volume. Our results show that plyometric training can stimulate cartilage thickness and volume in mice. This suggests further investigation of this mode of exercise as a countermeasure to prevent cartilage atrophy in disuse scenarios such as long duration spaceflight, and for patients at risk of developing osteoarthritis.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"5"},"PeriodicalIF":4.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11825961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143416194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validated space radiation exposure predictions from earth to mars during Artemis-I. 验证了“青蒿- 1”期间从地球到火星的空间辐射暴露预测。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-02-11 DOI: 10.1038/s41526-025-00459-y

Tony C Slaba, Shirin Rahmanian, Stuart George, Diego Laramore, John W Norbury, Charles M Werneth, Cary Zeitlin

引用次数: 0

Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flight. 月球和火星重力改变免疫细胞与内皮细胞在抛物线飞行中的相互作用。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-02-03 DOI: 10.1038/s41526-024-00456-7

Yu Du, Bing Han, Katharina Biere, Nathalie Abdelmalek, Xinyu Shu, Chaoyang Song, Guangyao Chen, Ning Li, Marina Tuschen, Huan Wu, Shujin Sun, Alexander Choukér, Mian Long, Dominique Moser

{"title":"Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flight.","authors":"Yu Du, Bing Han, Katharina Biere, Nathalie Abdelmalek, Xinyu Shu, Chaoyang Song, Guangyao Chen, Ning Li, Marina Tuschen, Huan Wu, Shujin Sun, Alexander Choukér, Mian Long, Dominique Moser","doi":"10.1038/s41526-024-00456-7","DOIUrl":"10.1038/s41526-024-00456-7","url":null,"abstract":"Returning to the moon and traveling to Mars represent the main targets of human space exploration missions within the upcoming decades. Comparable to microgravity, partial gravity in these destinations is assumed to dysregulate immune functions, thereby threatening astronauts´ health. To investigate the impact of partial gravity on immune cell attachment to vessel endothelia, THP-1 cells and HUVEC cell layers were monitored in a flow chamber system during parabolic flight in lunar (0.16 g) or Martian (0.38 g) gravity. Focus was set on floating speed, cell adhesion, surface molecule expression and cytoskeletal reorganization under basal and TNF-induced inflammatory environment. Floating speed of THP-1 cells was increased in partial gravity, which was accompanied by a successively lower adhesion to the endothelial HUVEC cells. Expression levels of the adhesion markers Mac-1 on THP-1 cells as well as ICAM-1 on HUVECs were found elevated in lunar and Martian gravity, which was aggravated by TNF. Analysis of cytoskeletal organization in HUVECs revealed reduced intracellular F-actin microfilament networks and a stronger cell directionality with stress fiber alignment at cell borders in partial gravity, which was intensified by TNF. In summary, altered immune cell - endothelium interactions as quantified in partial gravity conditions show similarities to cellular behavior in microgravity. However, the different magnitudes of effects in dependence of gravitational level still need to be assessed in further investigations.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"4"},"PeriodicalIF":4.4,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11791073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simulated deep space exposure on seeds utilizing the MISSE flight facility. 利用MISSE飞行设施模拟种子的深空暴露。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-01-17 DOI: 10.1038/s41526-024-00451-y

Jeffrey T Richards, Todd E Mortenson, Cory J Spern, Timothy A Mousseau, Jennifer L Gooden, Lashelle E Spencer, Christina L Khodadad, Jason A Fischer, Alexander D Meyers, Chad K Papenfuhs, Jeffrey G Buell, Howard G Levine, Dinah I Dimapilis, Ye Zhang

{"title":"Simulated deep space exposure on seeds utilizing the MISSE flight facility.","authors":"Jeffrey T Richards, Todd E Mortenson, Cory J Spern, Timothy A Mousseau, Jennifer L Gooden, Lashelle E Spencer, Christina L Khodadad, Jason A Fischer, Alexander D Meyers, Chad K Papenfuhs, Jeffrey G Buell, Howard G Levine, Dinah I Dimapilis, Ye Zhang","doi":"10.1038/s41526-024-00451-y","DOIUrl":"10.1038/s41526-024-00451-y","url":null,"abstract":"The MISSE-Seed project was designed to investigate the effects of space exposure on seed quality and storage. The project tested the Multipurpose Materials International Space Station Experiment-Flight Facility (MISSE-FF) hardware as a platform for exposing biological samples to the space environment outside the International Space Station (ISS). Furthermore, it evaluated the capability of a newly designed passive sample containment canister as a suitable exposure unit for biological samples for preserving their vigor while exposing to the space environment to study multi-stressor effects. The experiment was launched to the ISS on Northrup Grumman (NG)-15. The exposure lasted eight months outside the ISS in the MISSE-FF at the Zenith position. The specimens consisted of eleven seed varieties. Temperature dataloggers and thermoluminescent dosimeters were included in each container to record environmental data. We presented here the hardware and experimental design, environmental profiles, and seed survival from post-flight germination tests.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"3"},"PeriodicalIF":4.4,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypergravity is more challenging than microgravity for the human sensorimotor system. 对于人类的感觉运动系统来说，超重力比微重力更具挑战性。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-01-10 DOI: 10.1038/s41526-024-00452-x

Loïc Chomienne, Patrick Sainton, Fabrice R Sarlegna, Lionel Bringoux

引用次数: 0

Space exploration and risk of Parkinson's disease: a perspective review. 空间探索与帕金森病风险：观点综述。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-01-03 DOI: 10.1038/s41526-024-00457-6

Nilufar Ali, Afshin Beheshti, Greg Hampikian

引用次数: 0