npj Microgravity最新文献

Raman spectroscopy as a tool for assessing plant growth in space and on lunar regolith simulants. 拉曼光谱作为评估空间和月球风化模拟物上植物生长的工具。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-05-27 DOI: 10.1038/s41526-025-00479-8

Axell Rodriguez, Borja Barbero Barcenilla, Emily Hall, Ishan Kundel, Alexander Meyers, Sarah Wyatt, Dorothy Shippen, Dmitry Kurouski

引用次数: 0

Adoption of microfluidic MEA technology for electrophysiology of 3D neuronal networks exposed to suborbital conditions. 采用微流控MEA技术对亚眶环境下三维神经元网络进行电生理研究。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-05-27 DOI: 10.1038/s41526-025-00476-x

Andie E Padilla, Gobinath C, Candice Hovell, Jeremy Mares, Veerle Reumers, Twyman Clements, Jason Rextroat, Paul Gamble, Ben Lumpp, Binata Joddar

{"title":"Adoption of microfluidic MEA technology for electrophysiology of 3D neuronal networks exposed to suborbital conditions.","authors":"Andie E Padilla, Gobinath C, Candice Hovell, Jeremy Mares, Veerle Reumers, Twyman Clements, Jason Rextroat, Paul Gamble, Ben Lumpp, Binata Joddar","doi":"10.1038/s41526-025-00476-x","DOIUrl":"10.1038/s41526-025-00476-x","url":null,"abstract":"Studying neuronal cells in space reveals how microgravity affects brain function, gene expression, and cellular processes. This study details the preparation and validation of a 3D neuronal electrophysiology (EPHYS) sensing microfluidic biodevice used during a suborbital space flight. Initially, the device's function was tested with rat hippocampal neurons using EPHYS data collected via a microelectrode array (MEA). This system was later applied to human glutamatergic (Glu) neurons for eight days preceding a suborbital flight. A live-dead assay confirmed cell viability, and the system was integrated into a CubeLab to maintain a controlled environment. Two biological samples were flown, along with two control samples, to validate the EPHYS system. Results showed that human Glu-neurons exposed to microgravity exhibited altered expression of vesicular glutamate transporters (VGLUTs) while maintaining neuronal differentiation markers. The findings contribute to understanding neurological disorders, neuro-inflammation, and cognitive impacts of space travel, with broader applications for brain health research on Earth.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"20"},"PeriodicalIF":4.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survival strategies of Rhinocladiella similis in perchlorate-rich Mars like environments. 类似鼻枝菌在高氯酸盐丰富的火星环境中的生存策略。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-05-22 DOI: 10.1038/s41526-025-00475-y

Alef Dos Santos, Júnia Schultz, Felipe Oliveira Souza, Lucas Rodrigues Ribeiro, Thiago Verano Braga, Eduardo Jorge Pilau, Edson Rodrigues-Filho, Alexandre Soares Rosado

{"title":"Survival strategies of Rhinocladiella similis in perchlorate-rich Mars like environments.","authors":"Alef Dos Santos, Júnia Schultz, Felipe Oliveira Souza, Lucas Rodrigues Ribeiro, Thiago Verano Braga, Eduardo Jorge Pilau, Edson Rodrigues-Filho, Alexandre Soares Rosado","doi":"10.1038/s41526-025-00475-y","DOIUrl":"10.1038/s41526-025-00475-y","url":null,"abstract":"Studying the survival of terrestrial microorganisms under Martian conditions, particularly in the presence of perchlorates, provides crucial insights for astrobiology. This research investigates the resilience of the extremophile black fungus Rhinocladiella similis to magnesium perchlorate and UV-C radiation. Results show R. similis, known for its tolerance to acidic conditions, exhibits remarkable resistance to UV-C radiation combined with perchlorate, as well as to high concentrations of magnesium perchlorate, surpassing Exophiala sp. strain 15Lv1, a eukaryotic model organism for Mars-like conditions. Growth curve analyses revealed both strains can thrive in perchlorate concentrations mimicking Martian perchlorate-rich environments, with R. similis adapting better to higher concentrations. Morphological and protein production changes were investigated, and mass spectrometry identified perchlorate-induced proteins, advancing molecular understanding of potential microbial life on Mars. These findings advance knowledge of extremophile capabilities, contributing to the search for life beyond Earth and informing the design of future Martian rovers equipped for biosignature detection.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"18"},"PeriodicalIF":4.4,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The long-term stability of solid-state oral pharmaceuticals exposed to simulated intravehicular space radiation. 固体口服药物暴露于模拟舱内空间辐射的长期稳定性。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-05-17 DOI: 10.1038/s41526-025-00469-w

Ianik Plante, Vernie Daniels, Millennia Young, Ramona Gaza, Honglu Wu, John F Reichard

{"title":"The long-term stability of solid-state oral pharmaceuticals exposed to simulated intravehicular space radiation.","authors":"Ianik Plante, Vernie Daniels, Millennia Young, Ramona Gaza, Honglu Wu, John F Reichard","doi":"10.1038/s41526-025-00469-w","DOIUrl":"10.1038/s41526-025-00469-w","url":null,"abstract":"Pharmaceutical products brought for space missions must remain effective and safe throughout the mission. Previous NASA experiments suggest that radiation exposure could threaten drug stability during long-duration space missions. The Exploration Medical Capability (ExMC) Element has evaluated this possibility by exposing four medications to simulated Galactic Cosmic Radiation (GCRSim) at the NASA Space Radiation Laboratory followed by a three-year storage period. The solid oral drug products Acetaminophen, Amoxicillin, Ibuprofen, and Promethazine were used. Identical lots of each medication were assigned to four experimental groups: the non-irradiated Johnson Space Center control group, the non-irradiated traveling control group, the irradiation group I (GRSim, 0.5 Gy), and the irradiation group II (GCRSim, 1.0 Gy). Drug products were assessed for active pharmaceutical ingredient, degradation impurities, and dissolution 2, 18, and 34 months after irradiation. All samples show comparable degradation, revealing that GCR exposure does not facilitate the degradation of the drugs.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"17"},"PeriodicalIF":4.4,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144095769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The GENESTAR manual for biospecimen collection biobanking and omics data generation from commercial space missions. 生物标本收集、生物银行和商业太空任务组学数据生成的GENESTAR手册。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-05-14 DOI: 10.1038/s41526-025-00472-1

Aparna Krishnavajhala, Marie-Claude Gingras, Emmanuel Urquieta, Hsu Chao, Dilrukshi Bandaranaike, Yi Chen, Sravya Bhamidipati, Viktoriya Korchina, S Michelle Griffin, Michal M Masternak, Hannah Moreno, Javid Mohammed, Mullai Murugan, Jennifer E Posey, Jimmy H Wu, Donna Muzny, Richard A Gibbs, Harsha Doddapaneni

{"title":"The GENESTAR manual for biospecimen collection biobanking and omics data generation from commercial space missions.","authors":"Aparna Krishnavajhala, Marie-Claude Gingras, Emmanuel Urquieta, Hsu Chao, Dilrukshi Bandaranaike, Yi Chen, Sravya Bhamidipati, Viktoriya Korchina, S Michelle Griffin, Michal M Masternak, Hannah Moreno, Javid Mohammed, Mullai Murugan, Jennifer E Posey, Jimmy H Wu, Donna Muzny, Richard A Gibbs, Harsha Doddapaneni","doi":"10.1038/s41526-025-00472-1","DOIUrl":"https://doi.org/10.1038/s41526-025-00472-1","url":null,"abstract":"The surge in commercial and civilian spaceflight enables the systematic and longitudinal, large-scale biospecimen collection to understand the prospective effects of space travel on human health. The Genomics and Space Medicine (Space Omics) project at BCM-HGSC involves a comprehensive biospecimen collection plan from commercial/private space flight participants. The manuscript addresses the critical gaps in the biospecimen collection process including details of the informed consent process, a provision for subjects to obtain custom CLIA-WGS reports, a data dictionary and a LIMS enabled biobank. The manuscript also discusses the biospecimens collection, processing methodologies and nucleic acid suitability for Omics data generation. Results from Axiom-2 mission where, 339 biospecimens were collected using 'Genomic Evaluation of Space Travel and Research (GENESTAR)' manual, at two different sites, showed that 98% of the blood samples and 91.6% of the non-blood samples passed the QC requirements for Omics assays, underscoring the reliability and effectiveness of the GENESTAR manual.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"16"},"PeriodicalIF":4.4,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144082069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virtual reality-based sensory stimulation preferences at Amundsen-Scott South Pole station in Antarctica. 在南极洲阿蒙森-斯科特南极站基于虚拟现实的感官刺激偏好。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-05-11 DOI: 10.1038/s41526-025-00471-2

Stijn J J Thoolen, John-Michael Watson, Abigail M Fellows, Pooja P Bovard, Gary E Strangman, Jay C Buckey, Aleksandra S Stankovic

{"title":"Virtual reality-based sensory stimulation preferences at Amundsen-Scott South Pole station in Antarctica.","authors":"Stijn J J Thoolen, John-Michael Watson, Abigail M Fellows, Pooja P Bovard, Gary E Strangman, Jay C Buckey, Aleksandra S Stankovic","doi":"10.1038/s41526-025-00471-2","DOIUrl":"10.1038/s41526-025-00471-2","url":null,"abstract":"Virtual Reality (VR)-based sensory stimulation can provide relaxation and psychological restoration in isolated, confined and extreme conditions such as long-duration spaceflight, but it remains unclear which aspects of VR would be most beneficial. To investigate individual preferences for various VR attributes, 25 overwintering crew members at the Amundsen-Scott South Pole Station in Antarctica underwent 16 variations of VR stimulation at the end of their mission, with manipulations in delivery mode (VR vs. laptop), content (nature vs. city environments), duration (4 vs. 10 min), and sensory augmentation (with or without temperature cues). Data collection included pre- and post-intervention surveys on perceived quality (value, immersiveness, restorativeness) and mood, as well open-ended qualitative feedback. We found that VR was viable and restorative in a high-fidelity spaceflight analog. Although longer-lasting nature scenes were preferred overall, interindividual variation in preferences for sensory stimulation emphasizes the need for a personalized approach.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"15"},"PeriodicalIF":4.4,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144057170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brain and cerebrospinal fluid 3D center of mass shift after spaceflight. 航天飞行后脑和脑脊液三维质心偏移。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-05-08 DOI: 10.1038/s41526-025-00468-x

Katherine G Warthen, Stuart H Sater, Larry A Kramer, Khader M Hasan, Michael A Williams, Millennia Young, Brandon R Macias, Steven S Laurie, Bryn A Martin

{"title":"Brain and cerebrospinal fluid 3D center of mass shift after spaceflight.","authors":"Katherine G Warthen, Stuart H Sater, Larry A Kramer, Khader M Hasan, Michael A Williams, Millennia Young, Brandon R Macias, Steven S Laurie, Bryn A Martin","doi":"10.1038/s41526-025-00468-x","DOIUrl":"https://doi.org/10.1038/s41526-025-00468-x","url":null,"abstract":"A subset of long-duration spaceflight astronauts at the International Space Station has been documented to develop spaceflight associated neuro-ocular syndrome (SANS). Researchers have sought to understand SANS by quantification of ocular and brain structural changes thought to be associated with weightlessness induced headward fluid shift. Brain tissue shift and cerebrospinal fluid (CSF) redistribution has been observed as measured by MRI on return to Earth, and not fully quantified. To improve the understanding of this phenomenon, we developed and applied automated methods to quantify 3D center of mass shift within the skull of the extra-axial cerebrospinal fluid (eaCSF) and brain after long-duration spaceflight in astronauts (N = 13) and controls not exposed to microgravity (N = 10). 3D center of mass shift of brain tissue and CSF was computed based on registration of an individual skull segmentation at a baseline timepoint versus follow-up. 3D center of mass shift was quantified in the Gx, Gy, and Gz axis defined as -posterior/+anterior, -left/+right, -inferior/+superior, respectively. For astronauts, average MRI follow-up time pre- to post-flight was 697 ± 137 days (average flight duration = 179 ± 59 days with post-flight MRIs collected an average of 2.23 ± 1.64 days after return to Earth). For controls, average MRI follow-up time was 307 ± 19 days. For astronauts, a superior Gz shift in whole brain was present (+ 0.74 ± 0.28 mm, p < 0.0001) with a concomitant inferior Gz shift in eaCSF (-2.45 ± 0.99 mm, p < 0.0001). In the control cohort, brain tissue Gz shift (-0.082 ± 0.048 mm) and eaCSF Gz shift (0.096 ± 0.26 mm) were not statistically significant. Gy shift lacked significance in both controls and astronauts. These findings support that sustained exposure to weightlessness impacts the overall position of fluids and tissues within the skull.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"14"},"PeriodicalIF":4.4,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12058997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microscopy with microfluidics in microgravity using FlightScope. 使用FlightScope进行微重力下微流体显微镜观察。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-05-06 DOI: 10.1038/s41526-025-00470-3

Thomas Wareing, Alexander Stokes, Katrina E Crompton, Koren Murphy, Jack Dawson, Yusuf Furkan Ugurluoglu, Connor Richardson, Hongquan Li, Manu Prakash, Adam J M Wollman

引用次数: 0

Author Correction: Spaceflight causes strain-dependent gene expression changes in the kidneys of mice. 作者更正：太空飞行导致小鼠肾脏中菌株依赖性基因表达的变化。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-04-09 DOI: 10.1038/s41526-025-00467-y

Rebecca H Finch, Geraldine Vitry, Keith Siew, Stephen B Walsh, Afshin Beheshti, Gary Hardiman, Willian A da Silveira

引用次数: 0

Spaceflight causes strain-dependent gene expression changes in the kidneys of mice. 太空飞行导致小鼠肾脏中菌株依赖性基因表达的变化。

IF 4.4 1区物理与天体物理

npj Microgravity Pub Date : 2025-03-25 DOI: 10.1038/s41526-025-00465-0

Rebecca H Finch, Geraldine Vitry, Keith Siew, Stephen B Walsh, Afshin Beheshti, Gary Hardiman, Willian A da Silveira

{"title":"Spaceflight causes strain-dependent gene expression changes in the kidneys of mice.","authors":"Rebecca H Finch, Geraldine Vitry, Keith Siew, Stephen B Walsh, Afshin Beheshti, Gary Hardiman, Willian A da Silveira","doi":"10.1038/s41526-025-00465-0","DOIUrl":"10.1038/s41526-025-00465-0","url":null,"abstract":"Incidence of kidney stones in astronauts is a major risk factor associated with long-term missions, caused by increased blood calcium levels due to bone demineralisation triggered by microgravity and space radiation. Transcriptomic changes have been observed in tissues during spaceflight, including the kidney. We analysed kidney transcriptome patterns in two different strains of mice flown on the International Space Station, C57BL/6J and BALB/c. Here we show a link between spaceflight and transcriptome patterns associated with dysregulation of lipid and extracellular matrix metabolism and altered transforming growth factor-beta signalling. A stronger response was seen in C57BL/6J mice than BALB/c. Genetic differences in hyaluronan metabolism between strains may confer protection against extracellular matrix remodelling through the downregulation of epithelial-mesenchymal transition. We intend for our findings to contribute to the development of new countermeasures against kidney disease in astronauts and people here on Earth.","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"11"},"PeriodicalIF":4.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0