The effects of space radiation on the transcriptome of heart right ventricle tissue.

IF 4.4 1区物理与天体物理 Q1 MULTIDISCIPLINARY SCIENCES

npj Microgravity Pub Date : 2025-07-21 DOI:10.1038/s41526-025-00506-8

Roksana Zakharyan, Siras Hakobyan, Agnieszka Brojakowska, Malik Bisserier, Shihong Zhang, Mary K Khlgatian, Amit Kumar Rai, Suren Davitavyan, Ani Stepanyan, Tamara Sirunyan, Gisane Khachatryan, Susmita Sahoo, Venkata Naga Srikanth Garikipati, Arsen Arakelyan, David A Goukassian

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Abstract

Deep space represents a challenging environment for human exploration and can be accompanied by harmful health-related risks. We aimed to assess the effect of simplified galactic cosmic ray simulated (simGCRsim) and gamma (γ) ionizing radiation (IR) on transcriptome changes in right ventricular (RV) tissue after a single low dose (0.5 Gy, 500 MeV/nucleon) full body exposure in C57BL/6J male and female mice. In females, no differentially expressed genes (DEGs) and only 2 upregulated genes in males exposed to γ-IR were revealed. In contrast, exposure to simGCRsim-IR resulted in 4 DEGs in females and 371 DEGs in males, suggesting longer-lasting and sex-biased DEGs after simGCRsim-IR. Overrepresentation analysis of DEGs in simGCRsim-IR males revealed significant enrichment in pathways related to muscle contraction, hypertrophic cardiomyopathy, oxytocin release, the regulation of cytoskeleton, and genes associated with Alzheimer's, Huntington's, and Parkinson's diseases. Our results suggested the RV transcriptome exhibits distinct responses after exposure based on both the IR and sex.

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空间辐射对心脏右心室组织转录组的影响。

深空是人类探索的一个具有挑战性的环境，并可能伴随着有害的健康风险。我们旨在评估简化银河宇宙射线模拟（simGCRsim）和γ (γ)电离辐射（IR）对C57BL/6J雄性和雌性小鼠单次低剂量（0.5 Gy， 500 MeV/核子）全身暴露后右心室（RV）组织转录组变化的影响。在雌性中，暴露于γ-IR的雄性中没有差异表达基因（DEGs），只有2个基因表达上调。相比之下，暴露于simGCRsim-IR后，女性的deg为4，男性为371，表明simGCRsim-IR后的deg持续时间更长，性别有偏倚。对simGCRsim-IR男性中deg的过度代表性分析显示，与肌肉收缩、肥厚性心肌病、催产素释放、细胞骨架调节以及与阿尔茨海默病、亨廷顿病和帕金森病相关的基因相关的通路显著富集。我们的研究结果表明，RV转录组在暴露后根据IR和性别表现出不同的反应。

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来源期刊

npj Microgravity Physics and Astronomy-Physics and Astronomy (miscellaneous)

CiteScore

7.30

自引率

7.80%

发文量

审稿时长

9 weeks

期刊介绍： A new open access, online-only, multidisciplinary research journal, npj Microgravity is dedicated to publishing the most important scientific advances in the life sciences, physical sciences, and engineering fields that are facilitated by spaceflight and analogue platforms.