Materials Science & Engineering C-Materials for Biological Applications最新文献

{"title":"Functional replacement of fetal bovine serum by extracts from Galdieria sulphuraria in muscle cell culture","authors":"Svenja Hütker ,&nbsp;Hanna Wellkamp ,&nbsp;Verena Tellstroem ,&nbsp;Imke Lang ,&nbsp;Felicitas Berger","doi":"10.1016/j.bioadv.2025.214701","DOIUrl":"10.1016/j.bioadv.2025.214701","url":null,"abstract":"<div><div>The development of serum-free culture systems is a major challenge in the large-scale production of cultured meat, where myoblasts must be expanded and differentiated without the use of fetal bovine serum (FBS). FBS remains the gold standard in myoblast culture, yet it poses ethical, economic, and environmental concerns. In this study, we tested the hypothesis, that protein-rich extracts derived from the red microalga <em>Galdieria sulphuraria</em> can functionally replace FBS during proliferation and differentiation of murine C2C12 skeletal muscle myoblasts. Native algal protein extracts were prepared, heat-treated, and fractionated by ammonium sulfate precipitation. The effects of these extracts on cell viability, metabolic activity, proliferation kinetics and terminal myogenic differentiation were systematically assessed under serum-reduced and serum-free conditions. Crude extracts exhibited cytotoxicity, whereas heat-treated and fractionated extracts enhanced cell viability and metabolic activity during short-term culture. During long-term cultivation, gradual replacement of FBS with heat-treated algal extracts supported sustained myoblast growth, albeit with reduced proliferation rates, and preserved the capacity for differentiation into multinucleated, myosin heavy chain-positive myotubes. Comparative proteomic analysis revealed distinct protein profiles between crude and heat-treated extracts, suggesting that thermal processing removes inhibitory components while enriching bioactive protein fractions. Collectively, these results demonstrate that <em>G. sulphuraria</em>-derived protein extracts can support long-term myoblast proliferation and differentiation following appropriate adaption, highlighting their potential as a sustainable and animal-free alternative to serum-derived supplements in muscle cell culture systems.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214701"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevention of brain scarring during cranial reconstruction through a bioactive polymer coating","authors":"Sara Shakibania ,&nbsp;Daria Niewolik ,&nbsp;Taral Patel ,&nbsp;Małgorzata Bogusz ,&nbsp;Roman Turczyn ,&nbsp;Marcin Basiaga ,&nbsp;Katarzyna Jaszcz ,&nbsp;Barbara Bednarczyk-Cwynar ,&nbsp;Magdalena Skonieczna ,&nbsp;Katarzyna Krukiewicz","doi":"10.1016/j.bioadv.2025.214696","DOIUrl":"10.1016/j.bioadv.2025.214696","url":null,"abstract":"<div><div>Although cranioplasty is a long-established surgical procedure used to repair or reshape skull defects, it is not devoid of side effects arising from the formation of a scar tissue between dura, implant, and subcutaneous tissue. To minimize these complications, we developed a bioactive coating comprising poly(vinyl alcohol) (PVA) electrospun fibers loaded with a polyanhydride based on betulin disuccinate and a tricarboxylic derivative of poly(ethylene glycol) (PEG-DBB), suitable for the modification of the surface of titanium alloys. Although cell viability assays proved biocompatibility of fibers, PVA/PEG-DBB was found to decrease the adhesion of neuroblastoma cells by 84 % and the adhesion of fibroblast cells by 11 %, while increasing the confluency of osteosarcoma cells by 40 %. To assess the potential of the PVA/PEG-DBB fibrous coating, it was deposited onto the surface of Ti-6Al-4V alloy discs <em>via</em> electrospinning. <em>In vitro</em> bioactivity of samples was assessed <em>via</em> immersion in a simulated body fluid for 21 days, revealing facilitated formation of hydroxyapatite layer (Ca/P ratio of 1.74) in the presence of PVA/PEG-DBB (54.0 ± 6.5 % surface coverage, 11.7 ± 1.3 μm thickness), as confirmed by scanning electron microscopy and Raman spectroscopy. Adhesion strength of the coating to the substrate (between 1.7 and 2.7 N) was quantified using scratch testing under progressive loading, with critical load values determined to evaluate interfacial bonding integrity. By integrating biocompatibility, modulated adhesiveness, and mechanical resilience, PVA/PEG-DBB coating presents a promising approach for enhancing cranioplasty outcomes and minimizing adhesion-related complications.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214696"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel ROS switcher potentiates the type-I photodynamic effect of sodium zinc chlorophyllin against Pseudomonas aeruginosa in diabetic wounds","authors":"Guodong Wang ,&nbsp;Hongjie Chen ,&nbsp;Lusheng Wu ,&nbsp;Cai Yuan ,&nbsp;Mingdong Huang","doi":"10.1016/j.bioadv.2026.214721","DOIUrl":"10.1016/j.bioadv.2026.214721","url":null,"abstract":"<div><div>Skin wound infections present a serious threat to human health, with bacterial infections in diabetic wounds being particularly challenging due to impaired healing. Gram-negative bacteria are common pathogens in diabetic wound infections and often exhibit high resistance to conventional treatments, necessitating prolonged and costly therapies. Photodynamic antibacterial therapy (PACT) is regarded as a non-invasive and effective approach, with minimal risk of inducing resistance. Sodium zinc chlorophyllin (ZnChl), a water-soluble metalloporphyrin photosensitizer, offers multiple advantages such as low cost, high safety, and the ability to promote wound healing. However, its bactericidal efficacy against Gram-negative bacteria is limited under the hypoxic conditions typically found in diabetic wounds. Herein, we report a strategy to develop an efficient chlorophyll-based photosensitizer operating via an oxygen-independent Type I mechanism by combining ZnChl with inorganic salts (NaI, KBr, or KI). This approach significantly improved antibacterial performance, particularly with NaI or KI. Upon 630 nm light irradiation, low concentrations of KI (5 mM) markedly enhanced the bactericidal effect of ZnChl (50 μM), reducing the survival of three Gram-negative bacterial strains by 3 logs (99.9%). Interestingly, we further discovered that KI acts as a “reactive oxygen species (ROS) converter,” shifting the photodynamic mechanism of ZnChl from a mixed Type I/Type II mode to a dominant Type I mechanism, generating highly reactive hydroxyl radicals (<img>OH). Moreover, the ZnChl-KI combination demonstrated significant therapeutic efficacy in treating <em>Pseudomonas aeruginosa</em>-infected diabetic wounds without inducing apparent toxicity in rats. This work provides a foundation for developing efficient and economical Type I photosensitizers for the treatment of bacterial infections in diabetic wounds.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214721"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A four-pronged approach to unleash the regenerative potential of extracellular vesicles for bone regeneration: A systematic review of in vitro and in vivo studies","authors":"Shayan Salehy ,&nbsp;Hanieh Nokhbatolfoghahaei ,&nbsp;Arash Khojasteh","doi":"10.1016/j.bioadv.2025.214661","DOIUrl":"10.1016/j.bioadv.2025.214661","url":null,"abstract":"<div><div>The clinical application of Extracellular vesicles for bone regeneration faces several key challenges, including low production yield and potency, a lack of standardized isolation and characterization methods, and an incomplete understanding of their regenerative mechanisms, which render their clinical use ineffective. This systematic review addresses these limitations by elucidating regenerative mechanisms and identifying strategies to improve EV potency through an assessment of existing literature. A comprehensive search on MEDLINE, Scopus, and Web of Science yielded 133 articles, 72 (68 in vitro, 27 in vivo) of which met the inclusion criteria based on studies investigating methods to enhance EV bone regenerative potential. Data regarding EV source, affected cells and signaling pathways, study design, animal model, bone defect, administration route, and outcomes (bone formation and vascularization) were extracted and synthesized. Four distinct approaches were identified to improve EV bone regenerative potential at the cell source level: Targeted gene modification, chemical manipulation, Physical stimulation, and Culture-Based Optimization. While all included in vivo studies reported a significant increase in bone formation, none of the studies specified a method to eliminate bias (specifically selection bias, reporting bias, attrition bias, performance bias, and detection bias) in their studies; thus, any interpretations should be made with caution. Future studies should prioritize rigorous methodology centered around EV optimization, including targeted mechanisms for enhanced bone formation, and explore ways to improve clinical translatability. This systematic review was registered with <em>PROSPERO</em> (CDR42024511757).</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214661"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145864804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of osteogenic differentiation of boronized Ti6Al4V/HA composite involving TRIP13-PI3K/Akt signaling pathway","authors":"Yihong Chen ,&nbsp;Xiaojie Chen ,&nbsp;Boyu Liu ,&nbsp;Hengrong Xiong ,&nbsp;Zhiwei Peng ,&nbsp;Qian Peng","doi":"10.1016/j.bioadv.2026.214720","DOIUrl":"10.1016/j.bioadv.2026.214720","url":null,"abstract":"<div><div>Titanium and its alloys are widely used in dental implants owing to their excellent biocompatibility and corrosion resistance, despite their elastic moduli mismatch with bone and insufficient interfacial bioactivity which often restrict their osseointegration performance, particularly in the early stage after implantation. To address these limitations, a boronized Ti6Al4V/hydroxyapatite (HA) composite with improved structural and biological performance was developed. Scanning electron microscopy and atomic force microscopy confirmed a micro/nanostructured surface with increased roughness. RNA sequencing of osteoblasts cultured on the composite identified 683 upregulated and 838 downregulated genes relative to Ti6Al4V. Gene ontology enrichment revealed biological processes related to cell adhesion, extracellular matrix remodeling, and integrin-mediated signaling, whereas KEGG pathway analysis indicated activation of cell cycle, PI3K/Akt, and calcium signaling pathways. Gene set variation analysis further highlighted eight key upregulated genes-CYP1A1, CRLF2, HBEGF, IRAK2, DLL1, CYP1B1, BLOC1S5-TXNDC5, and TRIP13. Functional validation demonstrated that the TRIP13 expression correlated positively with osteogenic differentiation, in conjunction with activation of the PI3K/Akt signaling pathway. Collectively, these findings proved a TRIP13-associated transcriptional response linked to osteogenic regulation on the boronized Ti6Al4V/HA composite surface, offering a mechanistic insight into the design of bioactive titanium-based implants with improved osseointegration.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214720"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining cartilaginous microtissues primed under altered oxygen environments with melt electrowritten meshes to engineer scaled-up grafts","authors":"Nadia Rodriguez ,&nbsp;Inês F. Gonçalves ,&nbsp;Tom Hodgkinson ,&nbsp;Fergal J. O'Brien ,&nbsp;Daniel J. Kelly","doi":"10.1016/j.bioadv.2025.214680","DOIUrl":"10.1016/j.bioadv.2025.214680","url":null,"abstract":"<div><div>Current articular cartilage tissue engineering strategies fail to produce grafts that recapitulate the complex zonal composition and organisation of the native tissue. Developmental engineering strategies might overcome such limitations, leading to the generation of more biomimetic grafts by mimicking key steps of normal tissue development. One such approach leverages the capacity of stem/progenitor cells to self-organise into microtissues or organoids, which can be used as the building blocks of larger grafts. In this study, adult human bone marrow-derived mesenchymal stem/stromal cells (hMSCs) were used to engineer phenotypically distinct hyaline cartilage microtissues for the modular assembly of zonally defined grafts. Altered oxygen (O₂) levels, within the physiological range, were first explored to modulate the microtissue phenotype. Melt electrowritten (MEW) meshes were then used to guide the fusion of such microtissues into scaled-up grafts. Priming hMSC-derived microtissues at oxygen levels representative of different regions of the native tissue supported the development of distinct cartilaginous phenotypes. In addition, short-term exposure to 2 % O₂ significantly enhanced the deposition of glycosaminoglycans compared to exposure to 5 % O₂. Combining such microtissues with a supporting MEW mesh enabled the development of a larger graft with controlled geometry. In conclusion, this study highlights the potential of hMSC-derived cartilage microtissues, primed under altered oxygen environments, as building blocks for the biofabrication of articular cartilage grafts when combined with supporting MEW meshes.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214680"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145919006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted nanotheranostics mitigates radiation-induced fibrosis to promote immune infiltration and enhance radio-chemotherapy in pancreatic ductal adenocarcinoma","authors":"Arjun Sabu ,&nbsp;Ying-Chieh Yang ,&nbsp;Ritwick Ranjan Sarma ,&nbsp;Lakshminarayan Ramesan ,&nbsp;Yi-Hsuan Lin ,&nbsp;Hsin-Cheng Chiu","doi":"10.1016/j.bioadv.2025.214677","DOIUrl":"10.1016/j.bioadv.2025.214677","url":null,"abstract":"<div><div>Radiotherapy and chemotherapy exhibit limited clinical efficacy in pancreatic ductal adenocarcinoma (PDAC) due to its dense stromal desmoplasia, acting as a physical barrier that hinders drug penetration and immune cell infiltration while also promoting the formation of intratumoral radiation-induced fibrosis (RIF). In the present study, topoisomerase I inhibitor SN-38-loaded mesoporous silica-coated Bi₂O₃ nanoparticles (S-MBO NPs) were prepared and camouflaged with PDAC/red blood cell hybrid membranes loaded with the stromal reprogramming drug all-trans retinoic acid. The nanotherapeutics referred to as (S-MBO@A-RPCM NPs) were found capable of effectively overcoming the stromal barrier, mitigating RIF, and enhancing the radio/chemotherapeutic efficacy against PDAC. Both the <em>in vitro</em> and <em>in vivo</em> studies strongly confirmed the homotypic uptake and homologous targeting ability of S-MBO@A-RPCM NPs. The combined radiosensitizing effects of Bi₂O₃ and SN-38 resulted in enhanced DNA damage in UN-KC-6141 cells, decreased colony formation, and induction of immunogenic cell death by combined chemo/radiotherapy. Biodistribution data of S-MBO@A-RPCM NPs showed enhanced homologous targeting property toward PDAC, attributed to the hybrid membrane coating extracted from erythrocytes and UN-KC-6141 cancer cells on NPs. Importantly, S-MBO@A-RPCM NP treatment was found capable of deactivating the activated pancreatic stellate cells induced by radiation and TGF-β-thereby directly mitigating the fibrogenic activity characteristic of RIF <em>in vitro</em> and <em>in vivo</em>. Significantly reduced expression of α-SMA, collagen I, and fibronectin was observed after the treatment, accounting largely for the disruption of the stroma barrier, chronic inflammation signaling and tumor-stroma crosstalk, resulting in enhanced sensitization of UN-KC-6141 cells to SN-38 and radiation alongside improved immune infiltration. The <em>in vivo</em> data strongly signify that combining radiotherapy with the treatment of S-MBO@A-RPCM NPs exhibits superior tumor microenvironment reprogramming, attenuation of RIF and effective tumor growth inhibition in UN-KC-6141 tumor-bearing mice with minimal side effects.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214677"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145824127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel antimicrobial peptide AH-12 attenuates mitochondrial response to inflammatory stimuli and prevents periodontitis via antibacterial and anti-inflammatory effects","authors":"Yuanchen Wang ,&nbsp;Shuting Zhang ,&nbsp;Jinrong Sun ,&nbsp;Qian Zhang ,&nbsp;Xi Zhang","doi":"10.1016/j.bioadv.2026.214711","DOIUrl":"10.1016/j.bioadv.2026.214711","url":null,"abstract":"<div><div>Antibiotic-assisted periodontal therapy remains a primary clinical strategy, yet the rising prevalence of bacterial resistance severely compromises its therapeutic efficacy. To address this challenge, antimicrobial peptides (AMPs) have emerged as promising alternatives owing to their distinct antibacterial mechanisms. Here, we developed a novel antimicrobial peptide, AH-12, by systematically optimizing P-113—the minimal inhibitory fragment of human salivary Histatin 5—through N-terminal modification, amidation, and acetylation. <em>In vitro</em> evaluations demonstrated potent inhibitory effects of AH-12 against key oral pathogens (<em>Fusobacterium nucleatum</em>, <em>Porphyromonas gingivalis</em>, and <em>Streptococcus gordonii</em>), achieved via bacterial membrane lysis and suppression of FadA adhesin secretion in <em>F. nucleatum</em>. Beyond its antibacterial properties, AH-12 targeted inflammatory regulation by modulating mitochondrial function. Mitochondria, as early responders to inflammation, drive inflammatory cascades via Ca²⁺-dependent signaling. Remarkably, AH-12 stabilized mitochondrial Ca²⁺ levels, thereby attenuating mitochondrial reactive oxygen species (mtROS) overproduction and mitochondrial DNA (mtDNA) release, which collectively contributed to its robust anti-inflammatory effects. In order to minimize the loss of medication during administration and to better fit the shape of the periodontal pockets, a GelMA hydrogel (GelMA@AH-12) was engineered for controlled release applications. <em>In vivo</em> studies validated the outstanding efficacy of GelMA@AH-12, highlighting AH-12 as a transformative candidate for periodontitis treatment.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214711"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145978387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoplatform for renal ischemia-reperfusion injury repair: Modulating macrophage polarization, oxidative stress, and mitophagy","authors":"Mu He ,&nbsp;Ting Liu ,&nbsp;Hongchao Zhao ,&nbsp;Tianran Zheng ,&nbsp;Maonan Chen ,&nbsp;Hengcheng Zhu ,&nbsp;Kang Yang","doi":"10.1016/j.bioadv.2025.214697","DOIUrl":"10.1016/j.bioadv.2025.214697","url":null,"abstract":"<div><div>Acute kidney injury (AKI) is a common clinical condition characterized by high morbidity and mortality rates, with a notable lack of effective therapeutic drugs. Complex pathological processes—such as oxidative stress overload, aberrant macrophage polarization, mitochondrial dysfunction, and renal tubular epithelial cell apoptosis—contribute to the current absence of effective clinical treatments for AKI. Although mesoporous cerium dioxide nanospheres have been widely applied in various diseases due to their remarkable ROS-scavenging and drug-loading capabilities, their poor targeting ability limits their use in ischemia-reperfusion injury models. To address this, we developed a multifunctional nanoplatform RGD-CeO₂@Que. based on mesoporous hollow cerium dioxide (AhCeO₂). This system achieves targeted accumulation in injured kidneys by binding to integrin αvβ3 receptors, which are overexpressed under oxidative stress. Through the Nrf2/HO-1/GPX4/SOD1 pathway, it alleviates oxidative stress and reduces apoptosis. Moreover, the platform is loaded with the bioactive molecule quercetin to promote mitophagy in renal tubular epithelial cells (HK−2). In vivo studies demonstrated that RGD-CeO₂@Que. improves renal function, ameliorates pathological damage, and reduces inflammatory infiltration in AKI mice. In summary, this integrated nanoplatform combines multiple restorative mechanisms, offering a novel and targeted therapeutic strategy for AKI induced by renal ischemia-reperfusion injury.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214697"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amine functionalized TiO2 doped mesoporous bioactive glass coated curcumin infused 3d printed poly (L-lactic acid): A promising multifunctional scaffold with enhanced osteogenesis and tumour relapse inhibition characteristics","authors":"Shubham Pant ,&nbsp;Sravanthi Loganathan ,&nbsp;Ravi Babu Valapa","doi":"10.1016/j.bioadv.2026.214705","DOIUrl":"10.1016/j.bioadv.2026.214705","url":null,"abstract":"<div><div>The two main clinical problems with post-operative metastatic bone disease are high death rate and a notable local recurrence rate. When diseased bone sections are removed from patients, they suffer from significant bone defects that cannot be repaired on their own, and therefore require a support mechanism via the incorporation of scaffolds. In such cases, multifunctional scaffolds are deemed to be an appropriate option with promising potential as bone substitutes and limit the recurrence of post-operative tumour cells. This can be achieved via constructing a multi-functional scaffold incorporated with therapeutic compounds that helps in the prevention of tumour recurrence and improved surface characteristics for achieving enhanced osteogenesis. In the current work, a multi-functional scaffold is constructed via coating of amine functionalized Titanium dioxide (TiO₂) doped mesoporous bioactive glass (MBG) on 3D printed curcumin infused PLA support. The influence of curcumin incorporation towards inhibiting the growth of osteosarcoma cells and surface coating for enhanced osteogenesis of adipose-derived mesenchymal stem cells (AD-MSCs) is investigated. In this study, MBG is doped with TiO₂ and surface grafted with amine groups to facilitate coating on 3D printed PLA and curcumin infused PLA surface via EDC-NHS carbodiimide chemistry. As revealed by FESEM analysis, the bioactive coating was observed to induce <em>in-vitro</em> bioactivity on day 1 of SBF treatment. Most importantly, the release of curcumin shows antibacterial activity against gram-positive (∼90 %) and gram-negative bacterial strain (∼85 %) as well as cytotoxicity towards osteosarcoma cells lines (∼ 90 % cell apoptosis observed after 14 days of culture). The TiO₂ doped MBG promotes osteogenic differentiation, observed with a significant increase in calcium mineralization (<em>p</em> &lt; 0.0001) and Alkaline phosphatase activity (<em>p</em> &lt; 0.0001) compared to PLA scaffolds via mild release of reactive oxygen species, which facilitates production of antioxidant proteins and ultimately promotes upregulation of early and late osteogenic markers.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214705"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}