Materials Science & Engineering C-Materials for Biological Applications最新文献

{"title":"Alkaline phosphatase-instructed self-assembling supramolecular glucosamine hydrogel for osteoarthritis treatment","authors":"Li Lin ,&nbsp;Wenyan Qiu ,&nbsp;Jing Li ,&nbsp;Lingjun Zeng ,&nbsp;Changqing Zheng ,&nbsp;Xin Zhou ,&nbsp;Zhihong Liu ,&nbsp;Na Liu ,&nbsp;Xiaomu Hu ,&nbsp;Wenjun Tian","doi":"10.1016/j.bioadv.2025.214451","DOIUrl":"10.1016/j.bioadv.2025.214451","url":null,"abstract":"<div><div>Osteoarthritis (OA) is the most prevalent musculoskeletal disorder, affecting hundreds of millions of people worldwide. Glucosamine (GlcN) has been shown to effectively reduce proteoglycan degradation, attenuate articular cartilage degeneration and joint space narrowing, and alleviate osteoarthritis-related pain. However, the use of GlcN as a long-term medication for alleviating osteoarthritis is limited by its short half-life. Herein, we employed an enzyme-instructed self-assembly (EISA) strategy to construct a prodrug molecule, TP-(P)-G, containing a glucosamine (GlcN) moiety, which forms the supramolecular hydrogel TP-G under alkaline phosphatase (ALP) catalysis for OA treatment. Physicochemical characterization demonstrated that the hydrogel self-assembles into structurally stable nanofibrous networks exhibiting optimal viscoelastic behavior. In vitro evaluation revealed TP-G potently upregulated proteoglycan production in chondrocytes—with no significant cytotoxicity observed at biologically relevant doses. Subsequent in vivo studies established that TP-(P)-G administration significantly reduced key inflammatory cytokine concentrations, attenuated cartilage degeneration, and ameliorated synovitis and gait impairment in rats. Micro-CT and histological examinations provided further evidence of the hydrogel's protective effects on cartilage matrix and the subchondral bone interface, indicating that TP-G helps maintain the structural integrity of the joint. Additionally, H&amp;E staining of major organs revealed no observable pathological abnormalities, confirming the hydrogel's excellent biocompatibility and systemic safety. Collectively, these findings establish ALP-instructed self-assembly of TP-(P)-G into TP-G hydrogel as a disease-modifying strategy for OA, offering strong anti-inflammatory properties and chondroprotective benefits that could open new avenues for clinical intervention.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214451"},"PeriodicalIF":6.0,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144829396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin delivery and release by using a dual cooperative encapsulation system constructed with ZIF-8 MOFs and silk fibroin/polycaprolactone nanofibers for accelerated wound healing","authors":"Mahbubur Rahman ,&nbsp;Mohashin Kabir ,&nbsp;Hailei Liu ,&nbsp;Li Zhang ,&nbsp;Shaojuan Chen ,&nbsp;Shaohua Wu","doi":"10.1016/j.bioadv.2025.214441","DOIUrl":"10.1016/j.bioadv.2025.214441","url":null,"abstract":"<div><div>The development and design of novel nanofibrous dressings (NFDs) with biomimetic structure and multifunctional properties through electrospinning technique have aroused significant interest in the disciplines of wound treatment. In this study, curcumin (Cur) was firstly incorporated into zeolitic imidazolate framework-8 (ZIF-8), to generate Cur@ZIF-8 nanoparticles, which displayed a homogeneous, consistent, and nearly similar rhombic dodecahedral structure with the mean diameter of ~190 nm. Then the Cur@ZIF-8 was loaded into the silk fibroin (SF)/polycaprolactone (PCL) hybrid nanofibers to obtain a NFD through an electrospinning strategy. The other three different NFDs, <em>i.e.</em>, SF/PCL, SF/PCL/ZIF-8, and SF/PCL/Cur, were fabricated as control groups. All the four different NFDs were found to exhibit uniform and bead-free morphology. Among them, the SF/PCL/Cur@ZIF-8 NFD exhibited superior mechanical properties, high porosity (83.8 ± 3.7 %), and water uptake (292.4 ± 9.5 %). Importantly, the SF/PCL/Cur@ZIF-8 NFD showed a controlled Cur release profile, fitting the Krosmeyer-Peppas model. The <em>in vitro</em> cell experiments indicated that the SF/PCL/Cur@ZIF-8 NFD obviously improved the proliferation and adhesion of human dermal fibroblasts (HDFs). Moreover, the SF/PCL/Cur@ZIF-8 NDF also exhibited great antibacterial activity, with the inhibition rate of 83.2 ± 1.7 % and 80.2 ± 4.7 % against <em>E. coli</em> and <em>S. aureus,</em> correspondingly. <em>In vivo,</em> the SF/PCL/Cur@ZIF-8 NFD not only significantly reduced the bleeding amount in a mouse liver hemorrhage model, but also obviously accelerated the wound healing (~99 %) by augmenting the collagen growth and re-epithelialization. The present study revealed that the SF/PCL/Cur@ZIF-8 NFD generated by a combination of metal-organic framework (MOF) synthesis and electrospinning showed significant promise for applications in wound treatment and skin tissue engineering.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214441"},"PeriodicalIF":6.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144800808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Properties investigation on 3D-printed polyetherketoneketone (PEKK) – a proper candidate for bone replacement","authors":"Shuyuan Liu ,&nbsp;Tianyu Shu ,&nbsp;Yabo Zhao ,&nbsp;Feng Zhao ,&nbsp;Facheng Song ,&nbsp;Xiaoru Hou ,&nbsp;Yongquan Zhang ,&nbsp;Chaozong Liu ,&nbsp;Shuguang Liu ,&nbsp;Changning Sun ,&nbsp;Dichen Li ,&nbsp;Lei Shi ,&nbsp;Ling Wang","doi":"10.1016/j.bioadv.2025.214442","DOIUrl":"10.1016/j.bioadv.2025.214442","url":null,"abstract":"<div><div>Polyetherketoneketone (PEKK) has emerged as a highly promising material for bone substitutes due to its superior mechanical properties, biocompatibility, and patient-specific design capabilities. This study systematically investigated the mechanical properties of fused filament fabrication (FFF) 3D-printed PEKK in various printing orientations and found that PEKK specimens exhibited three times the tensile strength and flexural strength in the <em>Z</em>-axis compared to polyetheretherketone (PEEK). Surface assessments revealed that PEKK surfaces had greater roughness and wettability than those of PEEK under the same 3D printing conditions. In vitro biological evaluations of cell morphology and proliferation on PEKK surfaces demonstrated superior cell adhesion and cell proliferation compared to those of PEEK. Moreover, animal tests were conducted to assess their osseointegration performance followed by CT scanning, histological studies, and push-out tests. The CT scanning and histological results agree well with each other on the better bone quality and quantity surrounding the PEKK implants than those of the PEEK implants, which was consistent with the higher pushing-out force achieved in PEKK implant than those of the PEEK implant. In conclusion, the FFF 3D-printed PEKK is an ideal bone substitute material with outstanding mechanical and biological properties.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214442"},"PeriodicalIF":6.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144809622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipoic acid-modified epirubicin liposomal system for tumor-targeted drug delivery and cardiotoxicity reduction","authors":"Yanyan Han ,&nbsp;Chang Peng ,&nbsp;Zhanhong Qiao ,&nbsp;Xuan Wang ,&nbsp;Jin Peng ,&nbsp;Jingwen Liu ,&nbsp;Ming Zhao ,&nbsp;Dongkai Wang ,&nbsp;Haiyang Hu ,&nbsp;Dawei Chen","doi":"10.1016/j.bioadv.2025.214439","DOIUrl":"10.1016/j.bioadv.2025.214439","url":null,"abstract":"<div><div>This study aimed to address the bottlenecks of low delivery efficiency and high cardiotoxicity of anthracyclines by constructing liposomes of epirubicin functionalized with lipoic acid (Epi Lip@LA). This system was endowed with dual functions of dynamic targeting and microenvironmental response through lipoic acid modification: the lipoic acid group in DSPE-PEG2000-LA enhanced the uptake efficiency of tumor cells through dynamic covalent bond-mediated targeted delivery, and the high concentration of glutathione (GSH) in the tumor microenvironment triggered the specific cleavage of disulfide bonds to achieve precise drug release. Meanwhile, lipoic acid cooperatively blocked myocardial oxidative stress and inflammatory injury by scavenging reactive oxygen species, maintaining mitochondrial membrane potential stability, and inhibiting NLRP3 inflammasome activation. <em>In vivo</em> studies demonstrated that this system significantly enhanced the anti-tumor efficacy while effectively alleviating pathological changes such as myocardial fibrosis, achieving dual optimization of drug delivery efficiency and cardiac safety. This work provides an innovative strategy for developing nanocarrier systems with both efficient tumor targeting and systemic protective functions.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214439"},"PeriodicalIF":6.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144781063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A patient-derived decellularized extracellular matrix hydrogel as a biomimetic scaffold for advanced 3D colorectal cancer modeling","authors":"Edoardo D'Angelo ,&nbsp;Asia Marangio ,&nbsp;Francesca Sensi ,&nbsp;Eleonora Zanrè ,&nbsp;Agostino Steffan ,&nbsp;Ombretta Repetto ,&nbsp;Riccardo Rampado ,&nbsp;Monica Giomo ,&nbsp;Matteo Fassan ,&nbsp;Gaya Spolverato ,&nbsp;Elisa Cimetta ,&nbsp;Marco Agostini","doi":"10.1016/j.bioadv.2025.214438","DOIUrl":"10.1016/j.bioadv.2025.214438","url":null,"abstract":"<div><div>Colorectal cancer (CRC) is among the most prevalent cancers globally and is associated with a high mortality rate, particularly in advanced stages. In the realm of drug discovery, the use of innovative and highly translational pre-clinical CRC models is essential. Currently, the most relevant in vitro tumor approaches are three dimensional (3D) models. However, most 3D models of solid tumors are based either on synthetic materials or animal-derived commercial hydrogels, which fail to accurately mimic the biology of native tissues and originate from non-human sources. In contrast, hydrogels derived from human decellularized extracellular matrix (ECM) retain signaling cues from native tissue and represent a bioactive mechanical structure that can foster tumor cell growth in a tissue-specific 3D in vitro environment. Here, we demonstrated that patient-derived decellularized colon ECM can be processed into a hydrogel, producing the CologEM. CologEM formulation process preserved key ECM proteins, such as collagens, glycosaminoglycans and secreted bioactive molecules belonging to the family of cytokine, chemokine, interleukin, growth factors and ECM-remodeling enzyme. CologEM displayed a fibrous ultrastructure with interconnected pores, with notable differences observed between 1 % and 3 % (w/v) CologEM. Both 1 % and 3 % CologEM showed good biocompatibility, with 3 % CologEM demonstrating a higher propensity to induce a mesenchymal phenotype and resistance to antitumor drugs. In conclusion, CologEM is a suitable scaffold for 3D CRC models as it replicates critical characteristics of the tumor microenvironment. This model holds promise for facilitating the discovery and development of chemotropic drugs for cancer treatment.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214438"},"PeriodicalIF":6.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beeswax-enriched tricalcium phosphate/hydroxyapatite/sodium alginate/thymol 3D-printed scaffolds for application in bone tissue engineering","authors":"Martinho J. Francisco ,&nbsp;Cátia S.D. Cabral ,&nbsp;Paula Ferreira ,&nbsp;Ilídio J. Correia ,&nbsp;André F. Moreira","doi":"10.1016/j.bioadv.2025.214440","DOIUrl":"10.1016/j.bioadv.2025.214440","url":null,"abstract":"<div><div>Tissue engineering, particularly bone tissue engineering (BTE), continues to pose significant challenges to modern medicine. In this work, a rapid prototyping technique was explored to create 3D scaffolds using a Fab@Home 3D-Plotter extruder. For that purpose, a novel composite mixture containing tricalcium phosphate (TCP), hydroxyapatite (HAp), sodium alginate (SA), beeswax (BW), and thymol (TM) was formulated. BW and TM resulted in 3D scaffolds with rougher surfaces and moderate hydrophilic profiles, properties crucial for mediating cell adhesion and proliferation. Moreover, the 3D scaffolds containing BW displayed a significant increase in compressive strength and Young modulus, being comparable to those exhibited by trabecular bone. TM loading prevented the establishment of <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> infections, inhibiting bacterial adhesion and proliferation at the scaffolds' surface. Additionally, the cytocompatibility of the scaffolds was confirmed over 21 days, with the adhesion and proliferation of Human osteoblasts (hOB) at the scaffold's surfaces. Simultaneously, calcium and phosphate ions accumulated at the scaffolds' surface, forming apatite crystals. Therefore, this improved composite mixture showed promising results for being applied in BTE, not only facilitating hOB cell adhesion and proliferation but also avoiding bacterial infection, addressing a critical challenge in implant-based therapies.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214440"},"PeriodicalIF":6.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart zwitterionic biodegradable hydrogel for sustained peptide delivery: Application to the neurotherapeutic peptide NX210c","authors":"Elise Rosson ,&nbsp;Eloise Thomas ,&nbsp;Jacqueline Sidi-Boumedine ,&nbsp;David Kryza ,&nbsp;Marie Couderc ,&nbsp;Thomas Brichart ,&nbsp;Alain Geloen ,&nbsp;Alexandra Montembault ,&nbsp;Laurent David ,&nbsp;François Lux ,&nbsp;Yann Godfrin ,&nbsp;Olivier Tillement","doi":"10.1016/j.bioadv.2025.214437","DOIUrl":"10.1016/j.bioadv.2025.214437","url":null,"abstract":"<div><div>Therapeutic peptides offer a promising balance between specificity, safety, and bioavailability. However, their clinical use is limited by poor <em>in vivo</em> stability and short half-life, requiring frequent administrations that can compromise patient compliance and therapeutic outcomes. NX210c, a synthetic cyclic dodecapeptide derived from the thrombospondin repeat motif of the sub-commissural organ-spondin shows neuroprotective effects, making it a promising candidate for the treatment of neurological disorders. However, its short half-life (~15 min) requires intravenous infusion highlighting the interest of an alternative delivery method to improve the quality of life for patients.</div><div>This study, report the preclinical development of an injectable, biodegradable chitosan-based hydrogel functionalized with the macrocyclic ligand DOTAGA, designed for the sustained subcutaneous release of NX210c. The hydrogel forms <em>in situ</em> under physiological conditions, <em>via</em> electrostatic and hydrogen bonding interactions. The solvent-free formulation is simple and clinically compatible. Hydrogel candidate were screened <em>in vitro</em> for injectability, gelation, peptide loading, and release kinetics. <em>In vivo</em> validation on rodents confirmed subcutaneous injectability, gelation ability, biocompatibility and biodegradability within few weeks. Pharmacokinetics studies demonstrated distinct profiles: intravenous injection led to peptide clearance within 1 h; free NX210c administered subcutaneously slightly prolonged its presence in the bloodstream, with complete elimination observed within approximately 3 h. The use of hydrogel, enables AUCs dozens of times higher, with peptide levels sustained for over 10 to 15 days. These results highlight the hydrogel's potential as a patient-friendly, clinically translatable platform for therapeutic peptides requiring sustained release. Future studies will focus on the evaluation of its therapeutic efficiency.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214437"},"PeriodicalIF":6.0,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic silk fibroin meshes for regenerative soft tissue repair: Multiscale evaluation of a spatiotemporally active scaffold","authors":"Natasha Bokhari ,&nbsp;Asif Ali ,&nbsp;Sadaf Nausheen ,&nbsp;Abida Yasmeen ,&nbsp;Muhammad Awais Anjum ,&nbsp;Faiza Sharif","doi":"10.1016/j.bioadv.2025.214435","DOIUrl":"10.1016/j.bioadv.2025.214435","url":null,"abstract":"<div><h3>Background</h3><div>To address traditional/commercial surgical meshes linked complications like fibrosis, seroma, and bacterial infections, this study highlights the benefits of using lightweight, large-pore, all natural material meshes for abdominal wall healing, soft tissue repair and regeneration.</div></div><div><h3>Method</h3><div>This study presents a first-of-its-kind approach combining hand-knitted silk fibroin (SF) meshes with spin-assisted dip-coated biopolymer–phytochemical composites for soft tissue repair. The multifunctional mesh fabricated via a sustainable crochet method- weft hand-knitted silk fibroin (SF) meshes was surface-functionalized with spin-assisted dip biopolymer–phytochemical coatings. 5 % natural extracts (LE and BE) replace antibiotics, providing antimicrobial, antioxidant, and anti-inflammatory effects without toxicity. Tailored 12 % PHBV, PLA, and PCL coatings enable controlled drug release and extended degradation aligned with tissue repair. These modified meshes were analyzed in four phases i.e. material characterization, in silico assessments, in vitro testing, and in vivo analysis.</div></div><div><h3>Results</h3><div>Among the variants, PHBV modified meshes as an efficient drug delivery system with reduced pore size and increased fiber diameter (623.9 ± 66.7 μm and 12.1 ± 2.9 μm respectively). Specifically PHBV-LE emerged as the most effective composite with release kinetics of 70.4 % showing intermediate release of LE mediating the potent antimicrobial character. The PHBV-LE variant was a superior candidate with pore size promoting fibroblast proliferation (121.5 %), effective cell attachment, wound closure (88.27 %), and highly significant gene upregulation of key wound-healing markers (<em>MMP3</em>, <em>FGF-1</em>, <em>TGFβ-1</em>). In vivo analysis in rat models (Dawley) showed accelerated tissue integration and collagen deposition, indicating effective tissue repair and regeneration by PHBV-LE.</div></div><div><h3>Conclusion</h3><div>These meshes exhibited excellent material, antibacterial, and wound-healing properties, with lightweight structure, optimal pore size, and efficient drug delivery. Among all biomimetic SF composites, PHBV-LE emulates superior native extracellular matrix features, serving as multifunctional platforms for soft tissue repair and in vitro therapeutic evaluation.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214435"},"PeriodicalIF":6.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure, performance and tissue repair evaluation of biodegradable core-sheath fiber patch loaded with curcumin nano-micelles","authors":"Fuxun Qi ,&nbsp;Shasha Zhang ,&nbsp;Mingyu Zhang ,&nbsp;Yiru Xu ,&nbsp;Deng-Guang Yu ,&nbsp;Lei Chu ,&nbsp;Chenghao Wu ,&nbsp;Xiaoyan Li ,&nbsp;Xinliang Chen","doi":"10.1016/j.bioadv.2025.214432","DOIUrl":"10.1016/j.bioadv.2025.214432","url":null,"abstract":"<div><div>In this paper, curcumin nanomicelles (Cur@M) were uniformly encapsulated in the core-sheath polylactic acid (PLA) fibers using coaxial electrospinning technology, and the structure and properties were adjusted by post-processing to develop a multifunctional tissue repair patch. The optimized patch demonstrated sustained curcumin release, hydrophilic surface, enhanced mechanical strength and predicable degradation. The patch had excellent biocompatibility and exhibited significant anti-inflammatory efficacy by polarizing pro-inflammatory M1 macrophages to anti-inflammatory M2 phenotypes while inhibiting the production of reactive oxygen species (ROS) and nitric oxide (NO). <em>In vivo</em> evaluation showed that the patch was able to promote the regeneration of defective tissue while providing biomechanical strength comparable to that of natural autologous tissue. The therapeutic mechanism involves M2 macrophage-mediated anti-inflammatory response and enhanced synthesis of type I collagen (COL1), which is critical for tissue repair. This multifunctional patch shows significant potential in biomedical applications, especially in pelvic floor reconstruction and soft tissue engineering.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214432"},"PeriodicalIF":6.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144757255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of collagen matrix deposition in 2D cell cultures: a comparative study of existing assays","authors":"Syuzanna Hambardzumyan ,&nbsp;Jennifer Y. Kasper ,&nbsp;Aránzazu del Campo","doi":"10.1016/j.bioadv.2025.214436","DOIUrl":"10.1016/j.bioadv.2025.214436","url":null,"abstract":"<div><div>Collagen matrix deposition is an important biomarker to predict the regenerative capacity of new biomaterials or the therapeutic potential of new drugs in collagen-associated diseases. Several methods for the quantification of matrix collagen in tissue samples are established, e.g., Picro-Sirius red assay, hydroxyproline assay, antibody-based assays, or the 3,4-DHPAA-based assay. These methods have been extended to quantify deposited collagen in <em>in vitro</em> cell culture models, although their applicability has been questioned due to the much lower concentration and eventually lower relative abundance of deposited collagen in cell cultures than in tissue. Here we compare the performance of the above-mentioned methods for the quantification of deposited matrix collagen in 2D cell cultures under different conditions: culture time, addition of collagen deposition-stimulating molecules, and post-culture processing step (decellularization). We show that the available methods can deliver accurate results within different experimental windows. We provide a comprehensive analysis of the relevant experimental parameters that influence the assay, and the sensitivity limits for the different methods, as well as the involved effort. In a comparative table, we provide guidance for the selection of the most appropriate collagen quantification assay for different culture conditions.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214436"},"PeriodicalIF":6.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144809623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}