Materials Science & Engineering C-Materials for Biological Applications最新文献

{"title":"Cuttlebone inspired “S”-grooved topological structures facilitate osteogenic differentiation through the Rap1-ERK pathway","authors":"Ying Sun ,&nbsp;Yining Cai ,&nbsp;Shanshan Xu ,&nbsp;Ningning Zhai ,&nbsp;Zirui Wang ,&nbsp;Zongpu Qiu ,&nbsp;Shuying Han ,&nbsp;Yuanqing Wei ,&nbsp;Rui Liu ,&nbsp;Hao Wu","doi":"10.1016/j.bioadv.2025.214431","DOIUrl":"10.1016/j.bioadv.2025.214431","url":null,"abstract":"<div><div>While the regulatory roles of biomaterial topology in bone regeneration are recognized, the specific osteogenic mechanisms driven by distinct morphological features, particularly without biochemical cues, remain poorly understood. This study aimed to independently elucidate the osteogenic potential and underlying mechanisms solely attributable to the distinctive “S”-grooved topology derived from cuttlebone. Inspired by cuttlebone, we successfully fabricated a biomimetic polycaprolactone (PCL) membranes sheet with “S”-grooved topology using liquid silicone mold replication combined with PCL recasting techniques. Comprehensive characterization (SEM, CLSM) confirmed precise replication of the native “S”-grooved morphology (width: 100 ± 30 μm, depth: 20 ± 6 μm). In vitro studies revealed that these grooves promoted directional adhesion and proliferation of rat bone marrow mesenchymal stem cells (rBMSCs) via contact guidance, as evidenced by cytoskeleton alignment. Furthermore, the “S”-grooved topology significantly enhanced osteogenic differentiation of mouse pre-osteoblasts (MC3T3-E1), demonstrated by increased alkaline phosphatase (ALP) activity and mineralized nodule formation. Mechanistic investigations using RT-qPCR, Western blot, and dimethyl labeling-based quantitative proteomics on rBMSCs identified key involvement of the Rap1-ERK signaling pathway. Specifically, the “S”-grooved topology upregulated Rap1 expression and enhanced ERK phosphorylation, leading to increased expression of osteogenic markers (e.g., Runx2, OPN). These findings demonstrate that the biomimetic “S”-grooved topology alone, through contact guidance and activation of the Rap1-ERK mechanotransduction pathway, significantly enhances osteogenic differentiation, providing a foundation for designing topologically optimized biomaterials for bone regeneration biomimetic PCL membranes sheet with the “S”-grooved topology.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214431"},"PeriodicalIF":5.5,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COL4A2 drives ECM remodeling and stiffness increasing to promote breast cancer metastasis via YAP signaling pathway in dECM induced models","authors":"Jing Peng ,&nbsp;Weilai Zhu ,&nbsp;Chi Zhang ,&nbsp;Shuishui Yin ,&nbsp;Jie Ye ,&nbsp;Haijiao Mao ,&nbsp;Mei Li ,&nbsp;Jiyuan Zhao","doi":"10.1016/j.bioadv.2025.214430","DOIUrl":"10.1016/j.bioadv.2025.214430","url":null,"abstract":"<div><div>Mechanical stress significantly increases during tumor progression. Accumulated research focuses on mechanical transduction, due to the great therapeutic difficulties brought by the mechanical changes. Extracellular matrix (ECM) serves as the key tissue microenvironment providing mechanical cues for tumor cells. However, the mechanism of tumor ECM assembly, stiffness and the resulting cellular mechanical response were rarely reported. Here, decellularized ECM (dECM) models from low-metastatic and metastatic breast cancer tissues via in situ tumor implantation of mammary fat pad in immunodeficient mice were generated to simulate the tumor microenvironment. Wavy fiber structure, finer fibers, but higher stiffness were revealed in the metastatic dECM. Elevated expression of type IV collagen (COL IV) was correlated with the enhanced cell migration and the higher ECM stiffness due to the increased crosslinking of collagen fibers. Further analysis identified COL4A2 (a subunit of COLIV) as a key protein involved in this process. Virus infection of tumor cells led to a decrease in COL4A2 specificity in the dECM in situ, accompanied by the decreased ECM stiffness, the inhibition of cell migration in vitro, and the reduction of metastasis in vivo. Additionally, the increased ECM stiffness caused by the high content of COL4A2 in dECM scaffolds activated YAP1 expression, which might be a potential mechanism. Therefore, the promotion of the stiffness in the basement membrane by COL4A2 via collagen fiber cross-linking might be a key mechanical target for breast cancer metastasis. The targeting ECM mechanics could offer a new strategy to inhibit tumor progression.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214430"},"PeriodicalIF":5.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of a decellularized lung ECM-based bioink for bioprinting and fabricating a lung model","authors":"Nuraina Anisa Dahlan ,&nbsp;Kim Lam R. Chiok ,&nbsp;Xavier L. Tabil ,&nbsp;Xiaoman Duan ,&nbsp;Arinjay Banerjee ,&nbsp;Neeraj Dhar ,&nbsp;Xiongbiao Chen","doi":"10.1016/j.bioadv.2025.214428","DOIUrl":"10.1016/j.bioadv.2025.214428","url":null,"abstract":"<div><div>The construction of three-dimensional (3D) <em>in vitro</em> lung tissue models mimicking the physiological structure of the native lung poses a huge challenge in tissue engineering. While advances in bioprinting technology has made fabrication of 3D lung models feasible, the bioinks and printed constructs often fall short in achieving desired mechanical and biological properties. Toward this, we aimed to develop a novel bioink and use it to print and characterize <em>in vitro</em> 3D lung models with living cells. We generated porcine lung extracellular matrix (LdECM) which was then strategically combined with other hydrogels – alginate, carboxymethylcellulose (CMC), and collagen, to synthesize novel bioinks. The printability, mechanical and biological properties of the synthesized bioinks was characterized. We also characterized the rheological properties and identified the bioink composition – 3 % <em>w</em>/<em>v</em> alginate, 0.5 % w/v CMC, 0.5 mg/mL collagen Type 1 and 1 % <em>v</em>/v porcine LdECM was appropriate for bioprinting. To fabricate 3D lung models, we strategically designed and printed constructs featuring spatially organized patterns of MRC-5 human lung fibroblasts and A549-ACE2 human lung epithelial cells along with a cup-shaped structure to confine epithelial cells. Our results demonstrated that the bioinks with viscosities between 60 and 90 Pa.s were appropriate, which resulted in high printing resolution of cell-laden constructs and excellent cell viability. The bioprinted lung constructs also exhibited an elastic modulus of 2–4 kPa comparable to the stiffness of native lung tissues. Our findings establish a foundation for developing lung-specific 3D bioprinted models to address the growing global prevalence of respiratory diseases and for advancing preclinical therapeutic testing.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214428"},"PeriodicalIF":5.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of 3D and 2D cell-culturing methods in hair follicle spheroid morphogenesis and drug responsiveness","authors":"Justin J.Y. Tan ,&nbsp;Win Lwin Thuya ,&nbsp;Hongyu Zhu ,&nbsp;Jessie G. Kristo ,&nbsp;John E. Common ,&nbsp;Chunyong Wu ,&nbsp;Paul C.L. Ho ,&nbsp;Lifeng Kang","doi":"10.1016/j.bioadv.2025.214423","DOIUrl":"10.1016/j.bioadv.2025.214423","url":null,"abstract":"<div><div>Three-dimensional (3D) cell-culturing methods have usually been considered superior to two-dimensional (2D) culturing for in-vitro tissue formation intended for tissue engineering and drug research applications, including hair follicle (HF) development. However, cellular interactions within 3D cultures are generally more complex and therefore, may require further investigation. Apart from grafting in-vitro cultured (2D and 3D) dermal papilla cells directly onto the skin of animals to study the impact of 3D culturing on hair inductivity, molecular studies remain lacking in the understanding of how 2D and 3D culturing methods influence the morphogenesis of early stage HF models. The proposition that 3D cultures is always superior to 2D cultures for mimicking HF at its early developmental stage remains unknown. Therefore, this study aimed to investigate the influence of 3D and 2D culturing methods on the morphogenesis of HFs. 3D-cultured spheroids were assumed to exhibit greater expressions of HF-associated proteins and more expected drug-induced expression responses than 2D cultures. Dermal papilla cells and keratinocytes were cultured together in 2D and 3D cultures, where polyethylene glycol diacrylate microwell arrays were designed to provide the 3D culturing environment. Both 2D and 3D cultures were treated with either minoxidil or dihydrotestosterone (DHT) and the expressions of four hair proteins were analyzed.</div><div>The results showed that 3D cultures responded in more expected ways than 2D cultures when exposed to minoxidil, demonstrating a significant increase in trichohyalin (AE15, one of the 4 proteins) as expected, while 2D cultures exhibited a significant down-regulation. On the other hand, surprisingly, DHT treatment significantly reduced all protein expressions in 2D culture as expected, but did not significantly alter protein expression in 3D culture, suggesting that 2D cultures could respond better than 3D cultures in DHT treatment.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214423"},"PeriodicalIF":5.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Core-shell Zn/P-doped calcium silicate bioceramics with spatiotemporally regulated degradation and high-efficient oesteogensis for emergent bone trauma repair","authors":"Huiming Zhong ,&nbsp;Yan Xu ,&nbsp;Jiaqi Yang ,&nbsp;Zeshe Chen ,&nbsp;Min Cheng ,&nbsp;Jingqi Zheng ,&nbsp;Guangfeng Zhao ,&nbsp;Pengchao Guo ,&nbsp;Lei Zhang ,&nbsp;Zhongru Gou ,&nbsp;Guojing Yang ,&nbsp;Jian Shen","doi":"10.1016/j.bioadv.2025.214429","DOIUrl":"10.1016/j.bioadv.2025.214429","url":null,"abstract":"<div><div>Ca-silicate (CSi) bioceramics have garnered significant interest in bone tissue engineering but their multifunction and biodegradation are suboptimal for some emergent bone trauma conditions. Foreign ion doping and core-shell architectural design offer promising strategies to optimize osteogenic efficacy while precisely regulating degradation kinetics and biologically functional ion release. Herein we developed the core-shell porous bioceramics via coaxial nozzle system, featuring a P-doping wollastonite (CSi-P) core and Zn-doping wollastonite (CSi-Zn)/β-tricalcium phosphate (β-TCP) shells. A 10% porogens in the shell layer would enable controllable micropore architecture. In vitro studies demonstrated that Zn doping could finely tune the CSi-shell degradation rates, governing both physical dissolution and sustained release of bioactive ions. The CSi-based components further exhibited superior biomimetic remineralization capability in simulated body fluid. Meanwhile, both P12@Zn8 and P12@Zn12 exhibited remarkable antibacterial potential against Gram-positive bacteria (<em>S. aureus</em>). In vivo mandibular defect experiments revealed that the CSi-Zn granules outperformed β-TCP counterparts in bone repair at 10 and 16 weeks interval. Notably, the P12@Zn8 formulation achieved optimal degradation-osteogenesis coupling, exhibiting enhanced trabecular bone formation and complete repair within 16 weeks. This core-shell design strategically balances tunable degradation with spatiotemporal bioactivity, and may provide a solution to the problem of matching the absorption time of the materials with the bone regeneration time in clinical practice. Our findings highlight the potential of compositionally graded core-shell bioceramics as next-generation bioactive implants for emergent bone trauma regeneration and repair.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214429"},"PeriodicalIF":5.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Customized high-throughput microfluidic production of multifunctional porous microspheres for bone repair","authors":"Guangyu Hu ,&nbsp;Shuyun Zeng ,&nbsp;Tianao Shao ,&nbsp;Yong Li ,&nbsp;Yaoxian Han ,&nbsp;Jiawei Tu ,&nbsp;Jiahui Mao ,&nbsp;Yanbin Xiao ,&nbsp;Lei Zhang","doi":"10.1016/j.bioadv.2025.214426","DOIUrl":"10.1016/j.bioadv.2025.214426","url":null,"abstract":"<div><div>The development of bone repair materials has become a critical focus in addressing bone defects. Existing methods, such as autografts and allografts, are limited by issues like immune rejection and insufficient local anti-infection. Despite the promise of biocompatible scaffolds in drug and cell delivery, challenges persist in creating uniform, stable and injectable scaffolds with multifunctional properties for effective bone repair. Here, we present a customized coaxial microfluidic system designed for the high-throughput fabrication of PDTH porous microspheres (PDTH PMs). This system ensures precise control over microsphere size and structure, producing uniform, stable microspheres with interconnected pores that significantly enhance drug encapsulation efficiency. The PDTH PMs, composed of PLGA and functionalized with PDA and a hydrogel containing TOB and HA nanoparticles, demonstrate remarkable biocompatibility, ROS scavenging, and antibacterial properties. <em>In vitro</em>, these microspheres effectively promote BMSC proliferation, migration, and osteogenic differentiation. <em>In vivo</em>, using a rat calvarial bone defect model, PDTH PMs show significant improvement in bone regeneration, with increased bone mineral density and new bone formation. Biosafety <em>in vivo</em> assessments <em>via</em> histological examination and blood analysis confirm no adverse effects on major organs. Our work introduces a versatile method for bone defect repair, highlighting the potential of microfluidic-prepared multifunctional microspheres in bone regeneration strategies.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214426"},"PeriodicalIF":5.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosted breast cancer treatment with cell membrane-coated PLGA nanocarriers: Investigating the interactions with various cell types","authors":"Laís Ribovski ,&nbsp;Paula Maria Pincela Lins ,&nbsp;Bruna J. Moreira ,&nbsp;Luana C. Antonio ,&nbsp;Juliana Cancino-Bernardi ,&nbsp;Valtencir Zucolotto","doi":"10.1016/j.bioadv.2025.214420","DOIUrl":"10.1016/j.bioadv.2025.214420","url":null,"abstract":"<div><div>Nanomaterials inspired by nature and applied in the medical field have exhibited remarkable potential to diagnose and treat diseases. However, further improvements are required to enhance therapeutic effectiveness, particularly in terms of targeting. In this study, we formulated paclitaxel (PTX)-loaded poly lactic-<em>co</em>-glycolic (PLGA) nanocarriers (PLGA-PTX NCs) and coated with cancer cell membrane derived from MCF-7 breast cancer cells, mPLGA-PTX NCs. By leveraging the homotypic adhesion between cells, we enhance the treatment's effectiveness, which is associated with increased accumulation of mPLGA NCs in MCF-7 cells. Additionally, the cellular uptake of mPLGA NCs is investigated in cell types similar to MCF-7, which may also promote homotypic adhesion through specific adhesion molecules. This study includes A549 lung cancer cells, HDFn dermal fibroblasts, and MCF-10A non-tumorigenic breast cells. Our results show higher uptake for all cell lines, indicating homologous binding and common cell adhesion molecules, regardless of specificity. The treatment's efficacy, as evidenced by cellular metabolic activity, implies that both the percentage of NC-positive cells and uptake levels should be considered when evaluating therapeutic potency. All findings together emphasize the importance of thorough analysis of nanocarrier-cell interactions covering various cell types and understanding the nature of these interactions.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214420"},"PeriodicalIF":5.5,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A customizable micropatterned platform for osteosarcoma spheroid generation, imaging, and drug screening","authors":"Maria Veronica Lipreri ,&nbsp;Marilina Tamara Totaro ,&nbsp;Ilaria Raimondi ,&nbsp;Margherita Cortini ,&nbsp;Nicola Baldini ,&nbsp;Sofia Avnet","doi":"10.1016/j.bioadv.2025.214419","DOIUrl":"10.1016/j.bioadv.2025.214419","url":null,"abstract":"<div><div>Spheroids are three-dimensional cell clusters that serve as reliable in vitro models for cancer drug screening, mimicking tumor microarchitecture and chemoresistance. Despite their potential, current spheroid culture systems lack essential but highly challenging features, such as automatic real-time imaging, treatment response assessment, and detailed live image capturing without disturbing the spheroid structure. To address these challenges, we developed a custom culture dish with a micro-patterned agarose structure, fabricated from a 3D-printed mold. This innovative tool facilitates spheroid growth and immobilization, enabling automated high-throughput imaging and data collection. It allows the microscope objective to approach the spheroid closely (within micrometers) while it floats in the culture medium, in a mapped position. Furthermore, the platform is compatible with several imaging systems, including standard, confocal and dual-photon microscopy. We successfully demonstrated the effectiveness of our platform by culturing and treating osteosarcoma spheroids with different concentrations of a standard chemotherapeutic agent and by capturing confocal images of extracellular matrix antigens in live spheroids. Additionally, we showed that the platform is compatible with viability and metabolic assays (e.g. Alamar blue and acid phosphatase), with minimal reagent consumption and cost-effective, simultaneous imaging-based assays.</div><div>In conclusion, we propose an innovative platform for the study of tumor spheroids and other three-dimensional cellular structures, which allows tracking of size, shape, antigen expression, viability, and indirect monitoring of cell count over time. This advancement enhances our capacity to conduct in-depth investigations of cell behavior and therapeutic responses, contributing significantly to cancer research and drug development.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214419"},"PeriodicalIF":5.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring bioactive bone cement pastes: A promising solution for orthopedic surgeries","authors":"Afsaneh Jahani ,&nbsp;Mehrnoush Nakhaei ,&nbsp;Ali Moradi ,&nbsp;Mohammad Hossein Ebrahimzadeh ,&nbsp;Mahnaz sadat Mirbagheri ,&nbsp;Azar Gharib ,&nbsp;Fatemeh Koohzad ,&nbsp;Nafiseh Jirofti","doi":"10.1016/j.bioadv.2025.214416","DOIUrl":"10.1016/j.bioadv.2025.214416","url":null,"abstract":"<div><div>Bone cement is a biomaterial that has been used effectively in orthopedic surgery for almost 50 years. The cement hardens after injection in the defect site without needing any external agent. Bone cement is commonly used in implant fixation, joint replacement, trauma surgery, osteoporosis management, and antibiotic delivery. Since the bone anatomy of patients is different, the final hardening of bone cement in the body corrects the abnormalities and gaps between the implant and the bone. The most universal injectable bone cements are based on ceramic or polymer compounds. The chemical composition of ceramic cement is similar to that of bone and hardens by chemical reaction, while polymerization promotes hardening in polymer-based bone cement. Despite the significant progress of bone cement in orthopedic surgery, its limitations and accompanying considerations must be acknowledged. Long-term degradation potentially leads to loosening of the implant and an exothermic reaction during hardening, leading to tissue necrosis. To address these limitations various approaches have been continuously evaluated. The major objective of this review is to comprehensively evaluate ceramic and polymer-based bone cement as a promising approach in orthopedic surgery. Adequate knowledge can lead to the induction of strategies to improve the performance of bone cement.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214416"},"PeriodicalIF":5.5,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An early rapamycin-releasing nerve wrap with dual function for nerve regeneration and adhesion prevention","authors":"Naiyu Wang ,&nbsp;Shiqi Kang ,&nbsp;Qi Zeng ,&nbsp;Wu Xu ,&nbsp;Yuanyuan Zhao ,&nbsp;Xudong Shi ,&nbsp;Ruijun Li","doi":"10.1016/j.bioadv.2025.214411","DOIUrl":"10.1016/j.bioadv.2025.214411","url":null,"abstract":"<div><div>Limited ability of nerve regeneration and the formation of nerve adhesions result to poor outcomes of peripheral nerve injury (PNI) following surgical nerve repair. Currently, there is no efficient therapy for the improvement of functional recovery after surgical repair. Rapamycin (Rapa) has been found useful for promoting nerve regeneration through removing cellular debris at the early stages after PNI. However, its lower oral bioavailability and significant adverse effects limit its clinical application. In this study, we developed a biocompatible nerve wrap based on biocompatible monomethoxy poly (ethylene gly<em>co</em>l)-poly (lactic-<em>co</em>-glycolic acid) (mPEG-PLGA) for the local delivery of Rapa. The Rapa-mPEG-PLGA nerve wrap exhibited a rough surface and good hydrophilicity with strong mechanical strength. In vitro release studies revealed an initial rapid phase within the first 8 days, followed by a stable release about 10 days. The released Rapa remained its bioactivity to induce autophagy and promote cell migration. In a rat model of sciatic nerve transection and immediate repair, the implantation of Rapa-mPEG-PLGA nerve wrap significantly enhanced axonal regeneration and myelination. Furthermore, the nerve wraps effectively prevented the occurrence of nerve adhesions. Improved functional recovery was observed with Rapa-mPEG-PLGA membrane wrap post-surgery 4 weeks and 8 weeks. In summary, the Rapa-loaded mPEG-PLGA nerve wrap promoted nerve regeneration by releasing Rapa at the early stage after injury, and acted as a physical barrier to reduce nerve adhesions. This local dual-action strategy is a promising candidate for clinical translation in the treatment of PNI following surgical repair.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214411"},"PeriodicalIF":5.5,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}