Materials Science & Engineering C-Materials for Biological Applications最新文献

{"title":"Bacteriostatic, silver-doped, zirconia-based thin coatings for temporary fixation devices tuning stem cells' expression of adhesion-relevant genes and proteins","authors":"Sara Ferraris ,&nbsp;Alessandro C. Scalia ,&nbsp;Mauro Nascimben ,&nbsp;Sergio Perero ,&nbsp;Lia Rimondini ,&nbsp;Silvia Spriano ,&nbsp;Andrea Cochis","doi":"10.1016/j.bioadv.2025.214360","DOIUrl":"10.1016/j.bioadv.2025.214360","url":null,"abstract":"<div><div>Temporary fixation devices must support bone healing, be easily removed without bone tissue overgrowth, and reduce the risk of infection. To match these needs, mechanically and chemically stable thin coatings, based on a zirconia matrix doped with silver (ZrO2-Ag), were sputtered on Ti6Al4V. Coatings with two silver concentrations were produced: a low (0.2 % at Ag) concentration (AL) for bacteriostatic effect and a high (0.5 % at Ag) concentration (AH) for antibacterial properties. Surfaces were characterized for silver content and release, mechanical adhesion, morphology, roughness, wettability, and surface zeta potential, reporting high stability and a continuous Ag release over 28 days. Direct cytocompatibility was shown for human mesenchymal stem cells (hMSC), while antibacterial properties were verified towards <em>Staphylococcus aureus</em>. Results revealed non-toxic and anti-adhesion effects of AL that were deeply investigated towards hMSC by a multi-omics approach. Transcriptomics revealed a down-regulation of cadherins- and integrins-related genes involved in the cell-to-cell and cell-to-substrate adhesion, whereas proteomics confirmed a reduced expression of adhesion proteins (Talin and Ras homolog family member A - RhoA). The OMICS profiles were matched by bioinformatics analysis, confirming a cluster of preserved biological functions strongly related to the cells' adhesion but not to apoptosis. Therefore, AL is a good candidate for bone temporary fixation devices, not interfering with bone healing (cytocompatible), avoiding bone adhesion on the implant surface, and being bacteriostatic.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214360"},"PeriodicalIF":5.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled release of amitriptyline via the transdermal route using SmartReservoirs and hydrogel-forming microneedles","authors":"Abraham M. Abraham ,&nbsp;Amitha Simon ,&nbsp;Qonita Kurnia Anjani ,&nbsp;Yueming Jiang ,&nbsp;Masoud Adhami ,&nbsp;Juan Domínguez-Robles ,&nbsp;Eneko Larrañeta ,&nbsp;Ryan F. Donnelly","doi":"10.1016/j.bioadv.2025.214361","DOIUrl":"10.1016/j.bioadv.2025.214361","url":null,"abstract":"<div><div>SmartReservoirs (SRs) are novel drug reservoirs made from a cellulose-based matrix for hydrogel-forming microneedles (HF-MNs). SRs can incorporate drug molecules within a cellulose-based matrix in amorphous form. This significantly improves the solubility of poorly soluble drugs, which enhances drug delivery by allowing for rapid dissolution and absorption once the dosage form is administered. In contrast to improving the solubility of hydrophobic drugs, SRs might be used to modify the amorphous/crystalline properties of hydrophilic drugs, thus leading to a controlled release profile. Hence, SRs hold a promise as drug reservoirs for hydrophilic drugs and has not yet been investigated for such drugs; this study explores the transdermal delivery of the hydrophilic model drug amitriptyline hydrochloride (AMT) using SRs. Furthermore, the effect of the cellulose-based matrix on drug loading and release profile was also tested using tissue paper-based (SR-T) and copier paper-based (SR-P) SmartReservoirs. The current research involves the fabrication of HF-MNs and two types of AMT-SRs. Subsequently, a comprehensive characterisation of the HF-MNs and SRs was conducted regarding their morphological features, mechanical and physicochemical properties, amorphous/crystallinity state, interaction with the cellulose matrix, drug distribution, drug loading capacity, and transdermal permeation efficiency. The findings of the study demonstrated that the active pharmaceutical ingredient (API) remained intact within the cellulose matrices of both SRs. The type of cellulose matrix employed had a major influence on the loading and release of the drug. The SR-P demonstrated a significantly enhanced drug loading and release profile compared to the SR-T. The drug content analysis of the SRs revealed that SR-T had approximately 4 mg/cm² of AMT, in comparison to SR-P, which had a concentration nearly double that amount. The results of the skin deposition and permeation studies were also consistent, indicating that SR-T combined with HF-MNs deposited approximately 75 μg and permeated &lt;150 μg (~5 % delivered), while SR-P combined with HF-MNs deposited approximately 128 μg and permeated &gt;500 μg of AMT into the skin (~9 % delivered). Ultimately, this work provides substantial evidence to support the use of SRs as a hydrophilic drug reservoir for HF-MNs.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214361"},"PeriodicalIF":5.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory hydrogel loaded with PD-L1-expressing exosomes reprograms macrophages and accelerates diabetic wound healing","authors":"Mingzhu Zhai ,&nbsp;Hongxi Tan ,&nbsp;Aohong Xu ,&nbsp;Benqing Wu ,&nbsp;Fang Xie ,&nbsp;Yuanzhi Lu ,&nbsp;Yi Zheng","doi":"10.1016/j.bioadv.2025.214362","DOIUrl":"10.1016/j.bioadv.2025.214362","url":null,"abstract":"<div><div>Diabetic foot ulcers (DFU), a severe complication of diabetes mellitus, present a global healthcare challenge due to high risks of limb amputation and mortality. This study developed a multifunctional hydrogel dressing, Gelatin Methacryloyl (GelMA) hydrogel loaded with interleukin-4 (IL-4)-engineered exosomes (Exos), designed to synergistically modulate immune responses and enhance angiogenesis for complete diabetic wound repair. The programmed death-ligand 1 (PD-L1)-enriched Exos were engineered <em>via</em> IL-4 overexpression in NIH3T3 fibroblasts. The GelMA hydrogel loaded NIH3T3 fibroblast-derived Exos with IL-4 overexpression (GelMA/Exos^IL-4 hydrogel) exhibited favorable physicochemical characteristics, including a three-dimensional porous microstructure, injectability, tissue adhesion, self-healing properties, and sustained moisture retention. <em>In vitro</em> evaluation demonstrated biocompatibility, sustained exosome release, and enhanced viability, migration, and tube formation of human umbilical vein endothelial cells (HUVECs). In a diabetic wound model, the hydrogel significantly accelerated wound closure, promoted re-epithelialization and angiogenesis, and skewed macrophages toward anti-inflammatory M2 polarization while suppressing T-cell proliferation. These findings highlight the dual immunomodulatory and pro-angiogenic efficacy of GelMA/Exos^IL-4 hydrogel, offering a promising therapeutic strategy for chronic wound management.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214362"},"PeriodicalIF":5.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic enhancement of desferrioxamine and strontium-doped hydroxyapatite via coaxial electrostatic spinning on osteogenic differentiation","authors":"Yongman Liu ,&nbsp;Yubin Wang ,&nbsp;Wenxin Meng ,&nbsp;Wenjing Zhang ,&nbsp;Shuo Feng ,&nbsp;Shangquan Wu","doi":"10.1016/j.bioadv.2025.214342","DOIUrl":"10.1016/j.bioadv.2025.214342","url":null,"abstract":"<div><div>Defective bone repair is a pressing issue in the field of bone regeneration due to donor shortages and the associated complications of bone grafting. Artificial bone repair materials offer more options and are attracting extensive attention from researchers. However, achieving efficient osteogenesis in clinical practice remains a challenge. Here, dual drug-releasing nanofibre membranes (NMs) for bone repair was fabricated by coaxial electrostatic spinning. Strontium-doped hydroxyapatite (SrHA) and desferrioxamine (DFO) were loaded into the shell and core of the NMs for slow and sustained release, respectively. Our results showed that the DFO/SrHA NMs significantly enhanced cell proliferation, angiogenesis and osteogenic differentiation of rat bone mesenchymal stem cells through the synergism of DFO and SrHA. This synergy results from the slow and sustained release of DFO and SrHA via NMs, which maintains the drug concentration in the effective range for a long time, ensuring long-term stable activation of the HIF-1 <span><math><mi>α</mi></math></span> and Wnt/<span><math><mi>β</mi></math></span>-catenin signalling pathways. Overall, the DFO/SrHA NMs provides a promising strategy for defective bone repair by enhancing osteogenic differentiation.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214342"},"PeriodicalIF":5.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel off-liver sinusoidal progenitor cells in supporting transplantable bioliver engineering","authors":"Jiejuan Lai ,&nbsp;Deyu Hu ,&nbsp;Min Yan ,&nbsp;Quanyu Chen ,&nbsp;Hongbo Shi ,&nbsp;Qi Yang ,&nbsp;Mei Ding ,&nbsp;Lu Li ,&nbsp;Hongyu Zhang ,&nbsp;Lianhua Bai","doi":"10.1016/j.bioadv.2025.214358","DOIUrl":"10.1016/j.bioadv.2025.214358","url":null,"abstract":"<div><div>Liver transplantation is the only curative option for liver failure, which is currently limited by organ shortages. Although liver tissue engineering (LTE) with a strategy of decellularization/recellularization opens a new window to overcome this limitation, its challenges include the lack of specific seed cell sources and appropriate methods to support recellularization. In this preliminary study, both in vitro and in vivo experiments were performed. In vitro, we used novel off-liver progenitors of liver sinusoidal endothelial cells (LSECs) isolated from bone marrow mesenchymal stem cells (NG2/_BMMSCs) and a method to establish transplantable biolivers in an advanced decellularized liver scaffold (rDLS). This partial liver scaffolds (rDLS) shows advantages over whole liver scaffolds (nDLS) and also maintains both microvascular network and naturally regenerative microenvironmental niche, enhancing effective recellularization of the NG2/BMMSCs compared to nDLS in reconstructing hepatic main architectures of endothelial, sinusoidal and biliary tree before seeding hepatic stem cells (MLpvNG2 and ratOv cells). The recellularized hepatic stem cells-mediated bioliver was subsequently induced in a 3D bioreactor-like system with three different conditioned media (CM1–CM3) for different culture durations. Importantly, the NG2/_BMMSC-lined rDLSs effectively supported the functional bioliver engineering. In vivo, compared with control recipient pigs that underwent ∼90 % hepatectomy only, the recipients subjected to the same hepatectomy and received the bioliver presented greater survival durations. Overall, this study presents a new technology for fabricating a NG2/_BMMSC-based transplantable bioliver that may become a promising treatment for liver failure.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214358"},"PeriodicalIF":5.5,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144139582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid formulation alginate dressing with sustained nitric oxide release: a dual-action approach for antimicrobial and angiogenic benefits","authors":"Yifan Zhang ,&nbsp;Jiafeng Tan ,&nbsp;Xiaoliang Tu ,&nbsp;Dengfeng Tan ,&nbsp;Lanqin Yu ,&nbsp;Yijian Xie ,&nbsp;Na Li ,&nbsp;Shuyun Zhang ,&nbsp;Lihua Li ,&nbsp;Changren Zhou","doi":"10.1016/j.bioadv.2025.214350","DOIUrl":"10.1016/j.bioadv.2025.214350","url":null,"abstract":"<div><div>Infected chronic wounds resulting from bacterial, viral, or other microbial invasion disrupt the normal wound-healing process, often prolonging recovery and requiring surgical intervention. A high nitric oxide (NO) concentration damages bacterial DNA and disrupts metabolic functions, indicating its effective antibacterial potential, whereas a low NO concentration exerts detectable provascularization activity. These properties render NO an effective treatment agent for infected chronic wounds. However, challenges remain in generating NO efficiently, controlling its dosage, and selecting an appropriate carrier for wound treatment. To overcome these problems, we synthesized S-nitrosoglutathione (GSNO) and thiolated alginate (SA-SH). GSNO was used as the NO source for continuous release. The content of the thiolate group in SA-SH was 158.10 ± 10.84 μmol/g. The GSNO and SA-SH mixture was quickly solidified in the presence of calcium ions to obtain GSNO/SA-SH hydrogel (SSG). This mixture is suitable for treating irregular wounds. In addition, early release of high-dose NO inhibits bacteria (first 72 h), whereas late release of low-dose NO promotes angiogenesis (72–144 h). Experiments conducted on a rat infection model showed that the release of NO reduced infection and effectively inhibited bacterial invasion of tissues. On the 14th day after surgery, the healing rate of the control group was 82 %, whereas that of the SSG group was 100 %. The degree of angiogenesis in the SSG group was 1.5 times that in the SA-SH group, indicating that NO accelerates angiogenesis. Thus, a rapid-forming, NO releasing dressing can effectively treat infected wounds.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214350"},"PeriodicalIF":5.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rab27a-mediated extracellular vesicle release drives astrocytic CSPG secretion and glial scarring in spinal cord injury","authors":"Nidhi Singh ,&nbsp;Zarna Pathak ,&nbsp;Hemant Kumar","doi":"10.1016/j.bioadv.2025.214357","DOIUrl":"10.1016/j.bioadv.2025.214357","url":null,"abstract":"<div><div>Traumatic spinal cord injury (SCI) prevents axonal regeneration by impairing neuronal function and causing glial scarring. Chondroitin sulfate proteoglycans (CSPGs) from astrocytes drive this process, yet the release mechanism, potentially involving extracellular vesicles (EVs), remains unclear. Rab27a releases EVs from multivesicular bodies (MVBs) by enabling their docking and fusion with the plasma membrane. We confirmed Rab27a expression, and its localization, subsequently, EV release was validated with CD9, Alix, and TSG101 markers. Rab27a-mediated EV release was confirmed in both Rab27a-induced and Rab27a-siRNA-treated cells. Rab27a-derived EVs inhibited neuronal cell growth, while Rab27a-siRNA EVs promoted neuronal growth. Our study also observed upregulated Rab27a expression in the rat contusion model of SCI. Further analysis showed increased CSPG expression in Rab27a-induced conditions via the Rho/ROCK pathway, with altered pAkt, and β-tubulin III, levels. Immunohistochemistry confirmed CSPG/Rab27a/GFAP and CSPG/CD9 co-localization in tissue sections, verifying that Rab27a mediates EV release containing CSPG from astrocytes. These findings suggest that Rab27a plays a crucial role in CSPG release via EVs and scar formation. Functional recovery was significantly improved with Rab27a-siRNA treatment, suggesting Rab27a as a potential target for astrocytic scar modulation in SCI. The study reveals the detailed mechanistic insight of Rab27a-dependent CSPG release via EVs for sub-acute scar formation in contusion SCI.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214357"},"PeriodicalIF":5.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A facilely prepared dual-crosslinking adhesive with enhanced adhesive strength for hemostasis and infected wound healing","authors":"Ziyuan Guo ,&nbsp;Zongliang Wang ,&nbsp;Peibiao Zhang","doi":"10.1016/j.bioadv.2025.214349","DOIUrl":"10.1016/j.bioadv.2025.214349","url":null,"abstract":"<div><div>Although biological adhesives have shown advantages in replacing traditional wound suturing techniques, there are still limitations in wound closure, hemostasis, and healing, including insufficient tissue adhesion, potential biological toxicity, and complex preparation processes. In this study, a facile route for preparing injectable dual-crosslinking multifunctional hydrogel adhesive (Gel/EN/FBTA) was developed. The Gel/EN/FBTA adhesive is a dynamic cross-linked network composed of tannic acid (TA), 3-formylphenylboronic acid (3-FPBA) and gelatin, which can provide a large number of bonding sites and strengthen the adhesive cohesion through energy dissipation. The amidation reaction inside gelatin can form stable rigid crosslinks and maintain the structure of the adhesive stably. The balance between adhesion and cohesion can be regulated by adjusting the chemical composition and crosslinking density of the dual-crosslinking network. Under this equilibrium condition, the adhesion strength of Gel/EN/FBTA2 hydrogel is 3 times that of commercial fibrin glue, which shows good hemostatic effects in rat liver injury, rat tail injury, and rabbit liver cross incision models. Furthermore, Gel/EN/FBTA2 hydrogel adhesive can effectively treat wound infection, reduce inflammation level, promote re-epithelialization, accelerate collagen deposition, and achieve the healing of infectious full-thickness wounds. This dual-network design paradigm provides a versatile strategy for developing next-generation bioadhesives with tailored mechanical and bioactive properties, demonstrating significant potential for non-compressible hemorrhage and infected wound management.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214349"},"PeriodicalIF":5.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printing of an anatomically shaped bone model inspired by vascularized tubular bone structure","authors":"Elham Ghobadi ,&nbsp;Zahra Yahay ,&nbsp;Nima Nouri ,&nbsp;Fereshteh Karamali ,&nbsp;Elahe Masaeli","doi":"10.1016/j.bioadv.2025.214348","DOIUrl":"10.1016/j.bioadv.2025.214348","url":null,"abstract":"<div><div>Establishing early blood vessel networks within newly formed bone is vital for its survival and the successful integration of engineered bone grafts in living organisms. To tackle this challenge, we designed bone scaffolds with integrated microchannels within three-dimensional (3D) tissue structures, inspired by the principles of bio-vascularization. Using 3D printing, these structures replicate the natural bone vascular network, encompassing Haversian and Volkmann's arteries, showing significant potential for regenerating large-scale bone defects. We designed and fabricated three groups of structures with microchannels oriented vertically (Model A), vertical-horizontal (Model B), and in a vertical-horizontal-radial-central arrangement (Model C). Microstructure imaging revealed that 3D printing facilitates the development of bio-inspired structures with hollow microchannels, closely mimicking the vascular network of natural bone. Mechanical testing showed compressive strengths of 51, 41, and 37 MPa for structures A, B, and C, respectively. Importantly, all microchannels remained unobstructed, facilitating the transport of a blood-like fluid within the structures. These structures supported effective co-culture of MG63 cells within the spongy region and human umbilical vein endothelial (hUVECs) along the channels, demonstrating their ability to support cellular organization and vascularization. Overall, our research offers a versatile framework for designing and evaluating innovative 3D bio-inspired scaffolds specifically engineered for vascularized bone engineering. Among designed models, model C with vertical-horizontal-radial-central arrangement stands out due to its unique features and cohesive vascular network, making it highly suitable for advanced applications in bone tissue engineering.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214348"},"PeriodicalIF":5.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic-engineering Prussian blue hydrogel microspheres for enhanced osteoarthritis antioxidant therapy","authors":"Wangrui Peng ,&nbsp;Jie Lan ,&nbsp;MeeiChyn Goh ,&nbsp;Meng Du ,&nbsp;Zhiyi Chen","doi":"10.1016/j.bioadv.2025.214345","DOIUrl":"10.1016/j.bioadv.2025.214345","url":null,"abstract":"<div><div>Osteoarthritis (OA), a degenerative joint disorder and leading cause of global disabilty, imposes substantial societal and familial burdens. Current antioxidant therapies for OA are hindered by poor targeting and transient efficacy, failing to address the excessive reactive oxygen species (ROS)-driven pathogenesis. Herein, we innovatively integrate Prussian blue (PB) nanozymes with alginate-hyaluronic acid (HA) hydrogel microspheres through microfluidic engineering, creating the injectable AlgHA@PB platform that synergizes dual therapeutic mechanisms: ROS scavenging and oxygen generation via PB nanozymes, and sustained intra-articular retention and mechanical compatibility enabled by the hydrogel microsphere architecture. In vitro studies demonstrated that AlgHA@PB scavenged all of intracellular ROS while continuously releasing oxygen within. In a rat OA model, AlgHA@PB exhibited prolonged joint retention and reduced cartilage degeneration. Critically, the microspheres demonstrated a stable friction coefficient, enabling smooth intra-articular motion without mechanical irritation. This study establishes AlgHA@PB as a multifunctional OA therapeutic platform that integrates antioxidative defense, anti-inflammatory action, and biomechanical compatibility. The microfluidic-engineered design ensures scalable production, aligning with clinical translation requirements.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214345"},"PeriodicalIF":5.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}